Mutagenetix > Incidental Mutation

Incidental Mutation 'R6852:Fat1'

535031

Institutional Source

Beutler Lab

Gene Symbol

Fat1

Ensembl Gene

ENSMUSG00000070047

Gene Name

FAT atypical cadherin 1

Synonyms

mFat1, Fath, 2310038E12Rik

MMRRC Submission

045023-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6852 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

44935447-45052257 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 45035598 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 3472 (V3472A)

Ref Sequence

ENSEMBL: ENSMUSP00000149194 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098796] [ENSMUST00000189017] [ENSMUST00000191428] [ENSMUST00000215588]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000098796
AA Change: V3449A

PolyPhen 2 Score 0.295 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000096394
Gene: ENSMUSG00000070047
AA Change: V3449A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000189017
AA Change: V3449A

Predicted Effect

probably benign
Transcript: ENSMUST00000191428
AA Change: V3449A

PolyPhen 2 Score 0.295 (Sensitivity: 0.91; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000140596
Gene: ENSMUSG00000070047
AA Change: V3449A

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215588
AA Change: V3472A

PolyPhen 2 Score 0.460 (Sensitivity: 0.89; Specificity: 0.90)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit holoprosencephaly, anophthalmia, kidney defects and perinatal lethality. Mice homozygous for a hypomorphic allele exhibit altered shoulder girdle and facial musculature, retinal defects, abnormal inner earpatterning and kidney defects. [provided by MGI curators]

Allele List at MGI

All alleles(56) : Targeted, other(1) Gene trapped(55)

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	C	T	3: 138,065,169 (GRCm38)	Q40*	probably null	Het
1700034E13Rik	T	C	18: 52,663,633 (GRCm38)	V81A	probably damaging	Het
2310035C23Rik	T	C	1: 105,753,595 (GRCm38)	L1184P	probably damaging	Het
5330417C22Rik	T	C	3: 108,482,338 (GRCm38)	E203G	probably damaging	Het
9530053A07Rik	T	A	7: 28,147,135 (GRCm38)	I1084N	probably damaging	Het
A630089N07Rik	A	G	16: 98,065,655 (GRCm38)	I369T	probably benign	Het
Aatk	T	A	11: 120,010,468 (GRCm38)	Q977L	probably benign	Het
Abca4	A	T	3: 122,135,195 (GRCm38)	Q144H	probably damaging	Het
Ampd3	T	C	7: 110,802,546 (GRCm38)	F416L	probably benign	Het
Anks1b	A	G	10: 90,260,654 (GRCm38)	E425G	probably damaging	Het
Atm	A	G	9: 53,482,430 (GRCm38)	S1737P	possibly damaging	Het
Bsph1	T	A	7: 13,470,330 (GRCm38)		probably null	Het
Cabyr	C	T	18: 12,754,097 (GRCm38)	P262S	probably benign	Het
Cdc14a	G	A	3: 116,328,676 (GRCm38)	P214L	possibly damaging	Het
Cep128	C	A	12: 91,366,342 (GRCm38)		probably null	Het
Cfh	T	A	1: 140,147,749 (GRCm38)	R250S	probably damaging	Het
Ddx39	T	C	8: 83,723,017 (GRCm38)	I417T	probably benign	Het
Dgka	T	A	10: 128,722,539 (GRCm38)	K548M	probably damaging	Het
Efr3a	G	A	15: 65,829,830 (GRCm38)	V198I	probably benign	Het
Epg5	C	T	18: 78,012,891 (GRCm38)	T1760I	probably damaging	Het
Fbxo48	T	A	11: 16,953,402 (GRCm38)	N9K	probably benign	Het
Fer1l6	A	T	15: 58,594,878 (GRCm38)	H833L	probably damaging	Het
Foxn1	G	T	11: 78,360,960 (GRCm38)	Q482K	probably benign	Het
Fstl5	A	G	3: 76,707,855 (GRCm38)	D741G	probably damaging	Het
Gm8909	T	C	17: 36,168,073 (GRCm38)	N95D	possibly damaging	Het
Gmip	C	T	8: 69,817,991 (GRCm38)	Q55*	probably null	Het
Hapln2	G	A	3: 88,022,651 (GRCm38)	T306M	possibly damaging	Het
Kat6a	T	A	8: 22,938,660 (GRCm38)	S1344T	probably benign	Het
Kctd1	C	T	18: 14,986,344 (GRCm38)	D705N	possibly damaging	Het
Lhx9	ACC	ACCC	1: 138,841,806 (GRCm38)		probably null	Het
Micall2	G	T	5: 139,715,793 (GRCm38)	A482D	possibly damaging	Het
Mindy3	T	A	2: 12,419,252 (GRCm38)	M1L	possibly damaging	Het
Mtdh	A	G	15: 34,136,721 (GRCm38)	E352G	probably damaging	Het
Muc5ac	G	A	7: 141,816,907 (GRCm38)	V3219I	probably benign	Het
Myh4	A	G	11: 67,252,968 (GRCm38)		probably null	Het
Ncor1	A	G	11: 62,343,245 (GRCm38)	I377T	probably damaging	Het
Olfr1034	A	T	2: 86,046,604 (GRCm38)	I41F	probably benign	Het
Oog4	C	A	4: 143,439,109 (GRCm38)	C156F	possibly damaging	Het
Pcyt1a	T	C	16: 32,470,120 (GRCm38)	S260P	probably damaging	Het
Phf3	A	T	1: 30,804,630 (GRCm38)	F1749L	probably damaging	Het
Prickle2	T	C	6: 92,376,544 (GRCm38)	D647G	probably benign	Het
Raver2	T	C	4: 101,133,590 (GRCm38)	S387P	probably benign	Het
Rgs19	A	G	2: 181,689,148 (GRCm38)	S231P	possibly damaging	Het
Rpl8	G	T	15: 76,905,949 (GRCm38)	R198L	probably benign	Het
Sacs	T	A	14: 61,179,288 (GRCm38)	S77T	possibly damaging	Het
Samd14	G	A	11: 95,021,454 (GRCm38)	G219D	probably damaging	Het
Sec16a	C	T	2: 26,441,419 (GRCm38)	V195M	probably damaging	Het
Sesn2	C	T	4: 132,493,802 (GRCm38)	V453I	possibly damaging	Het
Slc46a3	A	T	5: 147,886,160 (GRCm38)	C291S	probably damaging	Het
Smarcal1	A	G	1: 72,591,173 (GRCm38)	T129A	possibly damaging	Het
Sorl1	T	A	9: 42,024,398 (GRCm38)	R1041W	possibly damaging	Het
Sptlc3	T	C	2: 139,566,586 (GRCm38)	I207T	probably benign	Het
Srgap3	T	C	6: 112,816,661 (GRCm38)	D118G	probably damaging	Het
Tas2r117	T	A	6: 132,802,929 (GRCm38)	V10D	probably benign	Het
Tbcd	A	G	11: 121,609,380 (GRCm38)	K1111E	probably benign	Het
Tor1aip1	A	T	1: 156,035,820 (GRCm38)	L139Q	probably damaging	Het
Trbv23	T	C	6: 41,216,247 (GRCm38)	V23A	probably damaging	Het
Ttll3	AAGTA	AAGTATAGTA	6: 113,399,159 (GRCm38)		probably null	Het
Ttll3	CAAAGTAA	CAAAGTAAAGTAA	6: 113,399,157 (GRCm38)		probably null	Het
Ttll3	GGCAAAG	GGCAAAGCAAAG	6: 113,399,155 (GRCm38)		probably null	Het
Vmn1r42	T	G	6: 89,844,787 (GRCm38)	T267P	possibly damaging	Het
Vmn2r53	C	A	7: 12,606,514 (GRCm38)	V11F	probably damaging	Het
Vmn2r67	T	C	7: 85,152,153 (GRCm38)	M192V	probably damaging	Het
Vmn2r78	T	C	7: 86,954,603 (GRCm38)	V663A	probably damaging	Het
Zdhhc1	C	T	8: 105,477,072 (GRCm38)	G156S	possibly damaging	Het
Zfp318	GAAGAA	GAAGAAAAAGAA	17: 46,412,533 (GRCm38)		probably benign	Het
Zfp318	AAGAAG	AAGAAGTAGAAG	17: 46,412,534 (GRCm38)		probably benign	Het
Zfp318	AGAAG	AGAAGAGGAAG	17: 46,412,538 (GRCm38)		probably benign	Het
Zfp560	A	G	9: 20,348,043 (GRCm38)	C508R	probably damaging	Het
Zfp654	G	A	16: 64,786,598 (GRCm38)	L414F	probably damaging	Het
Zfp872	A	T	9: 22,200,059 (GRCm38)	Y278F	probably damaging	Het
Zscan20	T	C	4: 128,589,722 (GRCm38)	S385G	probably damaging	Het

Other mutations in Fat1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Fat1	APN	8	45,024,602 (GRCm38)	missense	possibly damaging	0.93
IGL00157:Fat1	APN	8	44,951,670 (GRCm38)	missense	possibly damaging	0.96
IGL00481:Fat1	APN	8	45,050,940 (GRCm38)	missense	probably benign	0.18
IGL00983:Fat1	APN	8	45,033,390 (GRCm38)	missense	probably damaging	1.00
IGL01089:Fat1	APN	8	45,017,857 (GRCm38)	missense	probably damaging	1.00
IGL01135:Fat1	APN	8	45,024,840 (GRCm38)	missense	probably damaging	1.00
IGL01143:Fat1	APN	8	45,035,532 (GRCm38)	missense	possibly damaging	0.72
IGL01155:Fat1	APN	8	45,023,949 (GRCm38)	missense	probably damaging	1.00
IGL01376:Fat1	APN	8	45,026,841 (GRCm38)	missense	probably benign	0.00
IGL01411:Fat1	APN	8	45,026,800 (GRCm38)	missense	probably damaging	1.00
IGL01443:Fat1	APN	8	45,040,576 (GRCm38)	missense	probably damaging	1.00
IGL01453:Fat1	APN	8	45,051,270 (GRCm38)	missense	probably damaging	1.00
IGL01606:Fat1	APN	8	45,023,049 (GRCm38)	missense	probably benign	0.26
IGL01622:Fat1	APN	8	45,029,555 (GRCm38)	missense	possibly damaging	0.64
IGL01623:Fat1	APN	8	45,029,555 (GRCm38)	missense	possibly damaging	0.64
IGL01672:Fat1	APN	8	45,040,700 (GRCm38)	missense	probably benign	0.05
IGL01735:Fat1	APN	8	45,036,239 (GRCm38)	missense	probably benign	0.07
IGL01793:Fat1	APN	8	44,989,112 (GRCm38)	missense	probably benign
IGL01820:Fat1	APN	8	45,010,502 (GRCm38)	missense	probably damaging	1.00
IGL01969:Fat1	APN	8	44,952,599 (GRCm38)	missense	probably damaging	0.98
IGL02012:Fat1	APN	8	45,027,540 (GRCm38)	missense	possibly damaging	0.95
IGL02227:Fat1	APN	8	45,023,659 (GRCm38)	missense	probably damaging	1.00
IGL02256:Fat1	APN	8	44,950,332 (GRCm38)	missense	probably damaging	1.00
IGL02273:Fat1	APN	8	44,950,331 (GRCm38)	missense	probably damaging	1.00
IGL02317:Fat1	APN	8	45,025,818 (GRCm38)	missense	probably benign	0.33
IGL02324:Fat1	APN	8	45,040,556 (GRCm38)	missense	probably damaging	1.00
IGL02336:Fat1	APN	8	44,951,583 (GRCm38)	missense	probably benign	0.16
IGL02442:Fat1	APN	8	44,950,323 (GRCm38)	missense	probably benign	0.02
IGL02486:Fat1	APN	8	45,025,072 (GRCm38)	missense	probably benign	0.16
IGL02551:Fat1	APN	8	45,051,398 (GRCm38)	missense	probably damaging	1.00
IGL02617:Fat1	APN	8	45,035,591 (GRCm38)	missense	probably benign	0.31
IGL02698:Fat1	APN	8	45,023,164 (GRCm38)	missense	probably benign
IGL02885:Fat1	APN	8	44,989,167 (GRCm38)	missense	probably benign	0.01
IGL02904:Fat1	APN	8	45,040,682 (GRCm38)	missense	probably damaging	1.00
IGL02953:Fat1	APN	8	45,024,314 (GRCm38)	missense	probably damaging	1.00
IGL03108:Fat1	APN	8	45,023,614 (GRCm38)	missense	probably damaging	1.00
IGL03153:Fat1	APN	8	45,030,123 (GRCm38)	missense	possibly damaging	0.83
IGL03183:Fat1	APN	8	44,950,586 (GRCm38)	missense	probably damaging	0.99
IGL03327:Fat1	APN	8	44,950,468 (GRCm38)	missense	probably damaging	1.00
IGL03405:Fat1	APN	8	45,025,241 (GRCm38)	missense	probably damaging	1.00
Laggardly	UTSW	8	45,044,464 (GRCm38)	missense	probably damaging	1.00
R2257_fat1_465	UTSW	8	44,950,371 (GRCm38)	missense	probably damaging	1.00
Shrinkage	UTSW	8	45,018,037 (GRCm38)	missense	probably damaging	1.00
F5493:Fat1	UTSW	8	45,025,480 (GRCm38)	missense	probably damaging	0.99
G1citation:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
I2289:Fat1	UTSW	8	45,024,996 (GRCm38)	missense	probably benign	0.01
IGL02837:Fat1	UTSW	8	45,017,434 (GRCm38)	missense	probably benign	0.00
PIT4283001:Fat1	UTSW	8	45,037,207 (GRCm38)	missense	probably damaging	1.00
PIT4283001:Fat1	UTSW	8	45,029,540 (GRCm38)	missense	probably damaging	1.00
PIT4576001:Fat1	UTSW	8	45,024,645 (GRCm38)	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
R0078:Fat1	UTSW	8	44,953,299 (GRCm38)	missense	probably damaging	1.00
R0197:Fat1	UTSW	8	45,026,553 (GRCm38)	missense	probably benign	0.00
R0328:Fat1	UTSW	8	45,023,790 (GRCm38)	missense	probably benign	0.35
R0367:Fat1	UTSW	8	45,024,313 (GRCm38)	missense	probably damaging	1.00
R0371:Fat1	UTSW	8	44,951,892 (GRCm38)	missense	probably damaging	1.00
R0380:Fat1	UTSW	8	45,010,123 (GRCm38)	missense	probably damaging	0.97
R0389:Fat1	UTSW	8	44,950,348 (GRCm38)	missense	probably benign	0.00
R0433:Fat1	UTSW	8	45,024,649 (GRCm38)	missense	possibly damaging	0.51
R0456:Fat1	UTSW	8	45,029,534 (GRCm38)	missense	probably damaging	1.00
R0494:Fat1	UTSW	8	44,950,542 (GRCm38)	missense	probably damaging	1.00
R0506:Fat1	UTSW	8	45,022,951 (GRCm38)	missense	probably damaging	0.99
R0512:Fat1	UTSW	8	44,951,332 (GRCm38)	nonsense	probably null
R0624:Fat1	UTSW	8	45,051,168 (GRCm38)	missense	possibly damaging	0.46
R0701:Fat1	UTSW	8	45,026,553 (GRCm38)	missense	probably benign	0.00
R0723:Fat1	UTSW	8	45,026,749 (GRCm38)	missense	probably damaging	1.00
R0787:Fat1	UTSW	8	45,040,555 (GRCm38)	missense	probably damaging	1.00
R0788:Fat1	UTSW	8	45,023,983 (GRCm38)	missense	probably benign	0.27
R0862:Fat1	UTSW	8	45,018,037 (GRCm38)	missense	probably damaging	1.00
R0864:Fat1	UTSW	8	45,018,037 (GRCm38)	missense	probably damaging	1.00
R0907:Fat1	UTSW	8	45,026,598 (GRCm38)	missense	probably benign	0.08
R0962:Fat1	UTSW	8	45,033,326 (GRCm38)	splice site	probably benign
R1051:Fat1	UTSW	8	45,044,506 (GRCm38)	missense	probably damaging	1.00
R1156:Fat1	UTSW	8	45,039,890 (GRCm38)	missense	possibly damaging	0.94
R1237:Fat1	UTSW	8	45,044,279 (GRCm38)	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45,010,545 (GRCm38)	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45,010,545 (GRCm38)	missense	probably damaging	1.00
R1478:Fat1	UTSW	8	45,025,622 (GRCm38)	missense	probably damaging	0.99
R1482:Fat1	UTSW	8	44,953,244 (GRCm38)	missense	probably benign	0.04
R1496:Fat1	UTSW	8	45,033,390 (GRCm38)	missense	probably damaging	1.00
R1498:Fat1	UTSW	8	45,025,484 (GRCm38)	nonsense	probably null
R1508:Fat1	UTSW	8	45,026,862 (GRCm38)	missense	probably benign	0.01
R1577:Fat1	UTSW	8	45,023,383 (GRCm38)	missense	probably benign	0.30
R1646:Fat1	UTSW	8	45,018,042 (GRCm38)	missense	probably damaging	1.00
R1652:Fat1	UTSW	8	45,025,178 (GRCm38)	nonsense	probably null
R1656:Fat1	UTSW	8	45,025,530 (GRCm38)	nonsense	probably null
R1662:Fat1	UTSW	8	44,953,164 (GRCm38)	missense	probably benign	0.20
R1672:Fat1	UTSW	8	45,036,835 (GRCm38)	missense	probably damaging	1.00
R1704:Fat1	UTSW	8	45,025,576 (GRCm38)	missense	probably damaging	1.00
R1708:Fat1	UTSW	8	45,024,792 (GRCm38)	missense	probably damaging	1.00
R1710:Fat1	UTSW	8	45,010,482 (GRCm38)	missense	probably benign	0.00
R1812:Fat1	UTSW	8	45,036,803 (GRCm38)	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	45,038,349 (GRCm38)	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	44,953,304 (GRCm38)	missense	probably benign	0.01
R1883:Fat1	UTSW	8	45,051,147 (GRCm38)	missense	probably benign	0.17
R1893:Fat1	UTSW	8	45,023,856 (GRCm38)	missense	probably damaging	1.00
R1930:Fat1	UTSW	8	45,044,228 (GRCm38)	missense	possibly damaging	0.91
R1931:Fat1	UTSW	8	45,044,228 (GRCm38)	missense	possibly damaging	0.91
R1952:Fat1	UTSW	8	45,033,926 (GRCm38)	missense	probably benign	0.00
R1957:Fat1	UTSW	8	45,040,682 (GRCm38)	missense	probably damaging	1.00
R1999:Fat1	UTSW	8	44,952,393 (GRCm38)	missense	probably damaging	0.96
R2019:Fat1	UTSW	8	45,023,746 (GRCm38)	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45,026,704 (GRCm38)	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45,024,332 (GRCm38)	missense	probably damaging	1.00
R2117:Fat1	UTSW	8	45,037,463 (GRCm38)	missense	probably benign	0.33
R2196:Fat1	UTSW	8	45,024,646 (GRCm38)	missense	probably damaging	1.00
R2204:Fat1	UTSW	8	45,023,700 (GRCm38)	missense	probably damaging	1.00
R2256:Fat1	UTSW	8	44,950,371 (GRCm38)	missense	probably damaging	1.00
R2257:Fat1	UTSW	8	44,950,371 (GRCm38)	missense	probably damaging	1.00
R2409:Fat1	UTSW	8	45,040,530 (GRCm38)	splice site	probably benign
R2416:Fat1	UTSW	8	45,026,383 (GRCm38)	missense	probably damaging	1.00
R3021:Fat1	UTSW	8	45,044,011 (GRCm38)	missense	probably damaging	1.00
R3108:Fat1	UTSW	8	45,045,173 (GRCm38)	splice site	probably null
R3109:Fat1	UTSW	8	45,045,173 (GRCm38)	splice site	probably null
R3196:Fat1	UTSW	8	44,951,868 (GRCm38)	missense	probably benign	0.00
R3683:Fat1	UTSW	8	45,017,938 (GRCm38)	missense	probably benign
R3732:Fat1	UTSW	8	44,953,269 (GRCm38)	missense	possibly damaging	0.85
R3732:Fat1	UTSW	8	44,953,269 (GRCm38)	missense	possibly damaging	0.85
R3733:Fat1	UTSW	8	44,953,269 (GRCm38)	missense	possibly damaging	0.85
R3753:Fat1	UTSW	8	45,025,479 (GRCm38)	missense	probably damaging	0.97
R3905:Fat1	UTSW	8	45,023,035 (GRCm38)	missense	probably benign	0.00
R3907:Fat1	UTSW	8	45,023,035 (GRCm38)	missense	probably benign	0.00
R3908:Fat1	UTSW	8	45,023,035 (GRCm38)	missense	probably benign	0.00
R4060:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4061:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4062:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4063:Fat1	UTSW	8	45,025,481 (GRCm38)	missense	probably benign	0.09
R4078:Fat1	UTSW	8	44,989,122 (GRCm38)	missense	probably damaging	0.99
R4105:Fat1	UTSW	8	45,036,851 (GRCm38)	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45,050,944 (GRCm38)	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45,010,437 (GRCm38)	missense	probably damaging	1.00
R4161:Fat1	UTSW	8	45,036,787 (GRCm38)	missense	probably benign	0.00
R4364:Fat1	UTSW	8	44,952,962 (GRCm38)	missense	probably benign	0.01
R4394:Fat1	UTSW	8	44,952,346 (GRCm38)	missense	probably damaging	0.98
R4395:Fat1	UTSW	8	44,952,346 (GRCm38)	missense	probably damaging	0.98
R4396:Fat1	UTSW	8	44,952,346 (GRCm38)	missense	probably damaging	0.98
R4412:Fat1	UTSW	8	45,023,599 (GRCm38)	missense	probably damaging	0.99
R4542:Fat1	UTSW	8	45,041,894 (GRCm38)	missense	probably damaging	1.00
R4591:Fat1	UTSW	8	45,026,242 (GRCm38)	missense	probably benign
R4606:Fat1	UTSW	8	44,950,683 (GRCm38)	missense	possibly damaging	0.47
R4612:Fat1	UTSW	8	45,025,147 (GRCm38)	missense	probably damaging	1.00
R4730:Fat1	UTSW	8	45,033,477 (GRCm38)	missense	probably damaging	1.00
R4778:Fat1	UTSW	8	45,038,326 (GRCm38)	missense	probably benign	0.04
R4824:Fat1	UTSW	8	44,989,114 (GRCm38)	missense	probably damaging	1.00
R4829:Fat1	UTSW	8	45,036,162 (GRCm38)	missense	probably damaging	1.00
R4832:Fat1	UTSW	8	45,013,065 (GRCm38)	missense	possibly damaging	0.95
R4849:Fat1	UTSW	8	45,012,970 (GRCm38)	missense	probably benign	0.15
R4896:Fat1	UTSW	8	44,951,280 (GRCm38)	missense	possibly damaging	0.68
R4927:Fat1	UTSW	8	45,022,963 (GRCm38)	missense	probably damaging	0.96
R4941:Fat1	UTSW	8	45,036,275 (GRCm38)	missense	probably benign	0.00
R5011:Fat1	UTSW	8	45,031,263 (GRCm38)	critical splice acceptor site	probably null
R5040:Fat1	UTSW	8	45,023,380 (GRCm38)	missense	probably damaging	1.00
R5112:Fat1	UTSW	8	45,024,282 (GRCm38)	missense	probably damaging	1.00
R5151:Fat1	UTSW	8	44,951,814 (GRCm38)	missense	possibly damaging	0.74
R5161:Fat1	UTSW	8	44,952,512 (GRCm38)	missense	probably benign	0.00
R5162:Fat1	UTSW	8	45,025,809 (GRCm38)	missense	probably benign	0.02
R5353:Fat1	UTSW	8	45,036,131 (GRCm38)	missense	probably benign	0.13
R5425:Fat1	UTSW	8	45,025,885 (GRCm38)	missense	possibly damaging	0.64
R5458:Fat1	UTSW	8	45,013,053 (GRCm38)	missense	probably damaging	1.00
R5479:Fat1	UTSW	8	45,036,875 (GRCm38)	missense	possibly damaging	0.88
R5543:Fat1	UTSW	8	45,023,479 (GRCm38)	missense	probably damaging	0.99
R5569:Fat1	UTSW	8	45,039,836 (GRCm38)	missense	probably damaging	0.98
R5610:Fat1	UTSW	8	44,953,072 (GRCm38)	nonsense	probably null
R5734:Fat1	UTSW	8	45,051,209 (GRCm38)	missense	probably damaging	0.99
R5832:Fat1	UTSW	8	45,017,423 (GRCm38)	missense	possibly damaging	0.65
R5860:Fat1	UTSW	8	45,051,129 (GRCm38)	missense	probably benign
R5886:Fat1	UTSW	8	45,033,395 (GRCm38)	missense	probably damaging	1.00
R5886:Fat1	UTSW	8	45,027,681 (GRCm38)	critical splice donor site	probably null
R5919:Fat1	UTSW	8	45,026,873 (GRCm38)	critical splice donor site	probably null
R5930:Fat1	UTSW	8	45,044,036 (GRCm38)	missense	probably benign	0.10
R5960:Fat1	UTSW	8	45,033,368 (GRCm38)	missense	probably damaging	1.00
R5988:Fat1	UTSW	8	45,029,456 (GRCm38)	missense	probably benign	0.00
R6166:Fat1	UTSW	8	44,952,485 (GRCm38)	missense	probably damaging	1.00
R6184:Fat1	UTSW	8	44,953,392 (GRCm38)	missense	probably benign	0.00
R6208:Fat1	UTSW	8	45,027,613 (GRCm38)	missense	probably damaging	0.99
R6351:Fat1	UTSW	8	45,033,495 (GRCm38)	missense	probably damaging	1.00
R6391:Fat1	UTSW	8	44,952,342 (GRCm38)	missense	possibly damaging	0.69
R6701:Fat1	UTSW	8	44,950,681 (GRCm38)	missense	probably damaging	1.00
R6702:Fat1	UTSW	8	44,953,046 (GRCm38)	missense	probably benign	0.28
R6703:Fat1	UTSW	8	44,953,046 (GRCm38)	missense	probably benign	0.28
R6704:Fat1	UTSW	8	45,024,373 (GRCm38)	missense	probably damaging	1.00
R6822:Fat1	UTSW	8	45,026,404 (GRCm38)	missense	probably damaging	1.00
R6863:Fat1	UTSW	8	45,044,464 (GRCm38)	missense	probably damaging	1.00
R6885:Fat1	UTSW	8	44,952,452 (GRCm38)	missense	possibly damaging	0.94
R6912:Fat1	UTSW	8	45,051,023 (GRCm38)	missense	probably benign	0.00
R6927:Fat1	UTSW	8	45,024,495 (GRCm38)	missense	probably benign	0.41
R6964:Fat1	UTSW	8	45,043,945 (GRCm38)	missense	probably damaging	1.00
R7010:Fat1	UTSW	8	44,953,349 (GRCm38)	nonsense	probably null
R7062:Fat1	UTSW	8	44,950,216 (GRCm38)	start codon destroyed	probably null	0.99
R7063:Fat1	UTSW	8	45,040,775 (GRCm38)	missense	probably benign	0.09
R7071:Fat1	UTSW	8	44,989,108 (GRCm38)	missense	possibly damaging	0.67
R7117:Fat1	UTSW	8	45,031,468 (GRCm38)	missense	probably damaging	0.98
R7146:Fat1	UTSW	8	44,950,925 (GRCm38)	missense	probably benign
R7210:Fat1	UTSW	8	45,023,503 (GRCm38)	missense	probably damaging	1.00
R7227:Fat1	UTSW	8	45,010,609 (GRCm38)	missense	probably benign	0.08
R7270:Fat1	UTSW	8	45,037,438 (GRCm38)	missense	probably damaging	1.00
R7373:Fat1	UTSW	8	45,026,665 (GRCm38)	missense	probably damaging	1.00
R7390:Fat1	UTSW	8	44,952,474 (GRCm38)	missense	possibly damaging	0.81
R7465:Fat1	UTSW	8	45,044,152 (GRCm38)	missense	probably benign	0.35
R7476:Fat1	UTSW	8	45,031,274 (GRCm38)	missense	probably benign	0.01
R7483:Fat1	UTSW	8	45,023,160 (GRCm38)	missense	probably benign	0.13
R7484:Fat1	UTSW	8	45,036,184 (GRCm38)	missense	probably damaging	1.00
R7526:Fat1	UTSW	8	45,023,427 (GRCm38)	missense	probably damaging	1.00
R7549:Fat1	UTSW	8	44,988,994 (GRCm38)	missense	probably benign	0.01
R7554:Fat1	UTSW	8	45,037,165 (GRCm38)	missense	possibly damaging	0.88
R7620:Fat1	UTSW	8	45,009,850 (GRCm38)	missense	possibly damaging	0.95
R7652:Fat1	UTSW	8	44,953,299 (GRCm38)	missense	probably damaging	1.00
R7694:Fat1	UTSW	8	44,988,930 (GRCm38)	critical splice acceptor site	probably null
R7746:Fat1	UTSW	8	44,951,633 (GRCm38)	missense	probably damaging	0.96
R7762:Fat1	UTSW	8	45,037,337 (GRCm38)	missense	probably damaging	0.99
R7762:Fat1	UTSW	8	45,023,322 (GRCm38)	missense	probably damaging	1.00
R7782:Fat1	UTSW	8	44,950,911 (GRCm38)	missense	probably damaging	1.00
R7801:Fat1	UTSW	8	45,042,223 (GRCm38)	missense	probably damaging	1.00
R7807:Fat1	UTSW	8	45,041,973 (GRCm38)	missense	probably damaging	1.00
R7821:Fat1	UTSW	8	44,950,224 (GRCm38)	missense	probably benign
R7869:Fat1	UTSW	8	45,051,222 (GRCm38)	missense	probably benign	0.02
R8034:Fat1	UTSW	8	44,951,691 (GRCm38)	missense	probably benign	0.28
R8094:Fat1	UTSW	8	44,952,702 (GRCm38)	missense	probably damaging	0.98
R8111:Fat1	UTSW	8	45,026,058 (GRCm38)	missense	possibly damaging	0.94
R8220:Fat1	UTSW	8	45,039,956 (GRCm38)	missense	probably null
R8221:Fat1	UTSW	8	44,953,353 (GRCm38)	missense
R8233:Fat1	UTSW	8	44,952,018 (GRCm38)	missense
R8250:Fat1	UTSW	8	44,953,299 (GRCm38)	missense	probably damaging	1.00
R8279:Fat1	UTSW	8	45,030,347 (GRCm38)	critical splice donor site	probably null
R8726:Fat1	UTSW	8	45,024,169 (GRCm38)	missense	probably benign	0.23
R8875:Fat1	UTSW	8	45,040,563 (GRCm38)	missense	probably damaging	1.00
R8937:Fat1	UTSW	8	45,030,313 (GRCm38)	missense	probably damaging	1.00
R8950:Fat1	UTSW	8	45,023,121 (GRCm38)	missense	probably damaging	1.00
R8971:Fat1	UTSW	8	45,042,294 (GRCm38)	missense	probably damaging	1.00
R8976:Fat1	UTSW	8	45,031,295 (GRCm38)	missense	probably benign	0.02
R9000:Fat1	UTSW	8	45,044,550 (GRCm38)	nonsense	probably null
R9032:Fat1	UTSW	8	45,039,857 (GRCm38)	missense	probably benign	0.01
R9076:Fat1	UTSW	8	45,039,901 (GRCm38)	missense	probably damaging	1.00
R9083:Fat1	UTSW	8	45,038,299 (GRCm38)	missense	probably benign	0.00
R9083:Fat1	UTSW	8	45,013,090 (GRCm38)	missense	possibly damaging	0.76
R9103:Fat1	UTSW	8	44,951,813 (GRCm38)	missense	probably benign	0.38
R9124:Fat1	UTSW	8	45,025,027 (GRCm38)	missense	possibly damaging	0.48
R9124:Fat1	UTSW	8	44,950,326 (GRCm38)	missense	probably benign
R9128:Fat1	UTSW	8	45,009,841 (GRCm38)	missense	probably benign	0.14
R9148:Fat1	UTSW	8	44,952,645 (GRCm38)	missense	possibly damaging	0.81
R9162:Fat1	UTSW	8	44,951,315 (GRCm38)	missense	probably damaging	1.00
R9209:Fat1	UTSW	8	44,951,754 (GRCm38)	missense	possibly damaging	0.80
R9276:Fat1	UTSW	8	45,035,477 (GRCm38)	missense	probably damaging	0.99
R9303:Fat1	UTSW	8	45,010,461 (GRCm38)	missense	probably damaging	1.00
R9319:Fat1	UTSW	8	44,953,023 (GRCm38)	missense	probably damaging	1.00
R9392:Fat1	UTSW	8	45,023,191 (GRCm38)	missense	probably damaging	1.00
R9616:Fat1	UTSW	8	44,953,038 (GRCm38)	missense	probably damaging	0.99
R9712:Fat1	UTSW	8	45,017,380 (GRCm38)	missense	probably benign	0.05
R9756:Fat1	UTSW	8	45,043,937 (GRCm38)	missense	probably damaging	0.96
RF001:Fat1	UTSW	8	44,988,966 (GRCm38)	missense	probably benign	0.00
X0064:Fat1	UTSW	8	45,025,734 (GRCm38)	missense	possibly damaging	0.58
Z1088:Fat1	UTSW	8	45,023,807 (GRCm38)	missense	possibly damaging	0.88
Z1176:Fat1	UTSW	8	45,036,838 (GRCm38)	missense	probably damaging	1.00
Z1176:Fat1	UTSW	8	45,023,596 (GRCm38)	missense	possibly damaging	0.65
Z1176:Fat1	UTSW	8	44,950,598 (GRCm38)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CAAATGACGTCAGTGGTTCCC -3'
(R):5'- CCAAAGCACAGCATGGAGTG -3'

Sequencing Primer
(F):5'- CCGGTTGATTGGCACCATTCAG -3'
(R):5'- GAGGAATGCTGAGCTGAGTCCTAC -3'

Posted On

2018-09-12