Mutagenetix > Incidental Mutation

Incidental Mutation 'R6855:Lama1'

535223

Institutional Source

Beutler Lab

Gene Symbol

Lama1

Ensembl Gene

ENSMUSG00000032796

Gene Name

laminin, alpha 1

Synonyms

Lama

MMRRC Submission

045024-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6855 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67697265-67822645 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 67782155 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 1607 (Y1607C)

Ref Sequence

ENSEMBL: ENSMUSP00000043957 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035471]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000035471
AA Change: Y1607C

PolyPhen 2 Score 0.947 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000043957
Gene: ENSMUSG00000032796
AA Change: Y1607C

Domain	Start	End	E-Value	Type
low complexity region	3	22	N/A	INTRINSIC
LamNT	23	275	1.2e-131	SMART
EGF_Lam	277	331	1e-5	SMART
EGF_Lam	334	401	6.6e-6	SMART
EGF_Lam	404	458	9.11e-9	SMART
EGF_Lam	461	507	8.12e-6	SMART
LamB	570	702	2.09e-57	SMART
EGF_like	715	746	3.36e0	SMART
EGF_Lam	749	795	7.01e-10	SMART
EGF_Lam	798	853	3.59e-7	SMART
EGF_Lam	856	906	1.53e-10	SMART
EGF_Lam	909	955	1.13e-13	SMART
EGF_Lam	958	1002	1.36e-7	SMART
EGF_Lam	1005	1048	7.29e-8	SMART
EGF_like	1034	1082	4.83e1	SMART
EGF_Lam	1051	1094	1.67e-7	SMART
EGF_Lam	1097	1154	1.32e-5	SMART
LamB	1220	1352	8.7e-46	SMART
Pfam:Laminin_EGF	1367	1397	1.7e-6	PFAM
EGF_Lam	1410	1456	7.12e-11	SMART
EGF_Lam	1459	1513	3.25e-5	SMART
EGF_like	1497	1547	6.41e1	SMART
EGF_Lam	1516	1560	1.71e-13	SMART
Pfam:Laminin_I	1574	1838	1.7e-91	PFAM
low complexity region	2012	2031	N/A	INTRINSIC
low complexity region	2087	2098	N/A	INTRINSIC
LamG	2145	2287	3.66e-30	SMART
LamG	2332	2473	5.98e-35	SMART
LamG	2513	2661	1.11e-29	SMART
low complexity region	2695	2708	N/A	INTRINSIC
LamG	2743	2877	9.72e-35	SMART
LamG	2920	3056	4.63e-41	SMART

Meta Mutation Damage Score

0.3684

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.4%

Validation Efficiency

100% (57/57)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the alpha 1 subunits of laminin. The laminins are a family of extracellular matrix glycoproteins that have a heterotrimeric structure consisting of an alpha, beta and gamma chain. These proteins make up a major component of the basement membrane and have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Mutations in this gene may be associated with Poretti-Boltshauser syndrome. [provided by RefSeq, Sep 2014]
PHENOTYPE: Homozygous null mice display embryonic lethality before somite formation with impaired formation of Reichert's membrane. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aass	G	A	6: 23,114,845 (GRCm38)	S228F	probably damaging	Het
Cdh23	A	T	10: 60,306,122 (GRCm38)	M3062K	possibly damaging	Het
Cdh8	G	C	8: 99,190,217 (GRCm38)	S422C	probably damaging	Het
Col15a1	G	T	4: 47,245,544 (GRCm38)	K98N	probably damaging	Het
Cyp3a57	T	A	5: 145,372,566 (GRCm38)	L238H	probably damaging	Het
Dchs2	G	A	3: 83,348,194 (GRCm38)	R2399H	probably benign	Het
Dennd4c	A	C	4: 86,836,457 (GRCm38)	H1568P	probably benign	Het
Dkk2	T	G	3: 132,177,922 (GRCm38)	C194W	probably damaging	Het
Dnm2	C	T	9: 21,476,585 (GRCm38)	R369C	probably damaging	Het
Efcab7	T	G	4: 99,900,580 (GRCm38)	L308*	probably null	Het
Elp2	T	A	18: 24,606,877 (GRCm38)	N53K	possibly damaging	Het
Eml6	T	C	11: 29,751,381 (GRCm38)		probably null	Het
Erich3	G	T	3: 154,762,649 (GRCm38)	E913*	probably null	Het
Fam159b	T	C	13: 104,863,706 (GRCm38)	Q3R	probably benign	Het
Fam186a	T	A	15: 99,954,875 (GRCm38)	Y154F	unknown	Het
Fhl3	G	A	4: 124,707,522 (GRCm38)	D113N	probably benign	Het
Fryl	A	T	5: 73,059,500 (GRCm38)	M2149K	probably damaging	Het
Gbf1	T	C	19: 46,279,941 (GRCm38)	Y1251H	probably benign	Het
Ghitm	C	A	14: 37,125,902 (GRCm38)	G262W	probably null	Het
Gm14325	T	A	2: 177,832,842 (GRCm38)	H148L	probably damaging	Het
Grxcr1	C	A	5: 68,032,094 (GRCm38)	N69K	possibly damaging	Het
Kcnma1	A	G	14: 23,367,611 (GRCm38)	I846T	probably damaging	Het
Kdelc1	C	A	1: 44,110,827 (GRCm38)	E401*	probably null	Het
Lrp1b	G	C	2: 40,628,696 (GRCm38)	H175D	possibly damaging	Het
Lrrc2	T	A	9: 110,953,182 (GRCm38)		probably null	Het
Mpp3	A	G	11: 102,013,325 (GRCm38)	F291L	probably benign	Het
Nek7	C	T	1: 138,515,682 (GRCm38)	G178R	probably damaging	Het
Nfil3	T	A	13: 52,968,605 (GRCm38)	K88*	probably null	Het
Nit1	A	C	1: 171,343,562 (GRCm38)	V194G	probably damaging	Het
Nup210	T	C	6: 91,040,853 (GRCm38)	M1052V	probably benign	Het
Nup210l	A	G	3: 90,136,924 (GRCm38)	T511A	probably benign	Het
Nwd2	G	T	5: 63,804,451 (GRCm38)	M459I	probably benign	Het
Olfr1077-ps1	T	C	2: 86,526,169 (GRCm38)	N3D	probably benign	Het
Peg3	A	C	7: 6,708,798 (GRCm38)	S1142A	probably benign	Het
Phf3	T	A	1: 30,820,123 (GRCm38)	K959N	probably damaging	Het
Piezo2	C	T	18: 63,090,879 (GRCm38)		probably null	Het
Pigz	A	G	16: 31,945,218 (GRCm38)	I365V	possibly damaging	Het
Plec	T	A	15: 76,186,618 (GRCm38)	Q860L	probably damaging	Het
Ppp5c	T	G	7: 17,006,966 (GRCm38)	D359A	possibly damaging	Het
Pvr	C	T	7: 19,909,214 (GRCm38)	A365T	possibly damaging	Het
Pygm	G	T	19: 6,393,757 (GRCm38)	G583C	probably damaging	Het
Rasa3	A	T	8: 13,585,029 (GRCm38)	L430Q	probably damaging	Het
Rims2	T	G	15: 39,345,515 (GRCm38)	V198G	probably benign	Het
Rnf38	C	T	4: 44,149,224 (GRCm38)	R41H	probably damaging	Het
Rptn	G	A	3: 93,398,251 (GRCm38)	V964I	probably benign	Het
Rrp1b	A	G	17: 32,052,745 (GRCm38)	D263G	probably benign	Het
Sgip1	T	C	4: 102,962,376 (GRCm38)	C560R	probably damaging	Het
Sidt1	A	C	16: 44,245,343 (GRCm38)	I734S	probably null	Het
Ssfa2	C	T	2: 79,657,705 (GRCm38)	R711C	probably damaging	Het
Ssh1	G	T	5: 113,942,575 (GRCm38)	D931E	probably damaging	Het
Thumpd2	C	A	17: 81,044,170 (GRCm38)	R274I	probably damaging	Het
Tpi1	T	C	6: 124,812,463 (GRCm38)	D183G	probably damaging	Het
Trnt1	T	A	6: 106,777,922 (GRCm38)	Y205N	probably damaging	Het
Tsc22d2	G	A	3: 58,416,814 (GRCm38)	V376M	probably damaging	Het
Usp34	T	C	11: 23,452,569 (GRCm38)	V2454A	possibly damaging	Het
Uspl1	C	A	5: 149,187,845 (GRCm38)	S24Y	probably damaging	Het
Vmn1r58	A	C	7: 5,410,452 (GRCm38)	W260G	possibly damaging	Het
Vmn2r62	T	A	7: 42,788,988 (GRCm38)	L141F	probably benign	Het
Zfp868	A	C	8: 69,611,579 (GRCm38)	H368Q	probably damaging	Het

Other mutations in Lama1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Lama1	APN	17	67,815,928 (GRCm38)	missense	probably benign
IGL00336:Lama1	APN	17	67,813,948 (GRCm38)	missense	probably benign	0.07
IGL01066:Lama1	APN	17	67,743,326 (GRCm38)	missense	probably damaging	1.00
IGL01140:Lama1	APN	17	67,802,933 (GRCm38)	missense	probably benign	0.14
IGL01291:Lama1	APN	17	67,738,870 (GRCm38)	missense	probably damaging	1.00
IGL01296:Lama1	APN	17	67,745,051 (GRCm38)	missense	probably benign	0.27
IGL01317:Lama1	APN	17	67,818,701 (GRCm38)	missense	probably damaging	1.00
IGL01490:Lama1	APN	17	67,750,584 (GRCm38)	missense	possibly damaging	0.54
IGL01506:Lama1	APN	17	67,785,070 (GRCm38)	missense	probably benign	0.01
IGL01508:Lama1	APN	17	67,809,361 (GRCm38)	splice site	probably benign
IGL01522:Lama1	APN	17	67,752,774 (GRCm38)	splice site	probably benign
IGL01530:Lama1	APN	17	67,796,790 (GRCm38)	missense	probably benign	0.02
IGL01541:Lama1	APN	17	67,785,070 (GRCm38)	missense	probably benign	0.01
IGL01677:Lama1	APN	17	67,779,148 (GRCm38)	missense	probably benign	0.15
IGL01886:Lama1	APN	17	67,807,797 (GRCm38)	missense	probably benign	0.36
IGL01994:Lama1	APN	17	67,752,439 (GRCm38)	missense	probably benign	0.05
IGL02017:Lama1	APN	17	67,764,725 (GRCm38)	missense	probably benign	0.00
IGL02021:Lama1	APN	17	67,821,626 (GRCm38)	missense	probably damaging	1.00
IGL02026:Lama1	APN	17	67,809,292 (GRCm38)	missense	possibly damaging	0.82
IGL02044:Lama1	APN	17	67,811,490 (GRCm38)	missense	probably benign	0.01
IGL02120:Lama1	APN	17	67,716,789 (GRCm38)	missense	probably damaging	1.00
IGL02425:Lama1	APN	17	67,811,485 (GRCm38)	missense	probably benign	0.45
IGL02549:Lama1	APN	17	67,790,835 (GRCm38)	missense	possibly damaging	0.93
IGL02642:Lama1	APN	17	67,812,366 (GRCm38)	missense	probably benign	0.00
IGL02795:Lama1	APN	17	67,738,894 (GRCm38)	splice site	probably null
IGL02798:Lama1	APN	17	67,795,191 (GRCm38)	splice site	probably benign
IGL02863:Lama1	APN	17	67,804,536 (GRCm38)	missense	probably damaging	0.99
IGL02870:Lama1	APN	17	67,804,536 (GRCm38)	missense	probably damaging	0.99
IGL02876:Lama1	APN	17	67,750,692 (GRCm38)	critical splice donor site	probably null
IGL02885:Lama1	APN	17	67,804,536 (GRCm38)	missense	probably damaging	0.99
IGL02891:Lama1	APN	17	67,804,536 (GRCm38)	missense	probably damaging	0.99
IGL02978:Lama1	APN	17	67,786,081 (GRCm38)	nonsense	probably null
IGL03064:Lama1	APN	17	67,779,104 (GRCm38)	missense	probably benign	0.01
IGL03076:Lama1	APN	17	67,716,799 (GRCm38)	missense	possibly damaging	0.95
IGL03110:Lama1	APN	17	67,798,986 (GRCm38)	missense	probably benign	0.04
IGL03143:Lama1	APN	17	67,804,536 (GRCm38)	missense	probably damaging	0.99
IGL03159:Lama1	APN	17	67,804,536 (GRCm38)	missense	probably damaging	0.99
IGL03268:Lama1	APN	17	67,804,536 (GRCm38)	missense	probably damaging	0.99
ANU05:Lama1	UTSW	17	67,738,870 (GRCm38)	missense	probably damaging	1.00
PIT4472001:Lama1	UTSW	17	67,764,704 (GRCm38)	missense
R0047:Lama1	UTSW	17	67,795,186 (GRCm38)	splice site	probably benign
R0047:Lama1	UTSW	17	67,795,186 (GRCm38)	splice site	probably benign
R0050:Lama1	UTSW	17	67,782,056 (GRCm38)	missense	possibly damaging	0.66
R0096:Lama1	UTSW	17	67,805,413 (GRCm38)	missense	probably benign	0.12
R0096:Lama1	UTSW	17	67,805,413 (GRCm38)	missense	probably benign	0.12
R0111:Lama1	UTSW	17	67,737,498 (GRCm38)	missense	probably damaging	0.98
R0116:Lama1	UTSW	17	67,776,923 (GRCm38)	missense	probably benign	0.10
R0121:Lama1	UTSW	17	67,798,513 (GRCm38)	splice site	probably benign
R0278:Lama1	UTSW	17	67,810,183 (GRCm38)	missense	probably null	0.98
R0281:Lama1	UTSW	17	67,817,569 (GRCm38)	missense	probably damaging	1.00
R0312:Lama1	UTSW	17	67,775,851 (GRCm38)	missense	possibly damaging	0.45
R0419:Lama1	UTSW	17	67,791,610 (GRCm38)	critical splice donor site	probably null
R0512:Lama1	UTSW	17	67,779,134 (GRCm38)	missense	possibly damaging	0.67
R0514:Lama1	UTSW	17	67,764,698 (GRCm38)	missense	probably benign	0.40
R0562:Lama1	UTSW	17	67,815,959 (GRCm38)	missense	probably damaging	1.00
R0632:Lama1	UTSW	17	67,752,368 (GRCm38)	splice site	probably benign
R0645:Lama1	UTSW	17	67,773,712 (GRCm38)	missense	probably benign	0.01
R0712:Lama1	UTSW	17	67,779,042 (GRCm38)	splice site	probably null
R0763:Lama1	UTSW	17	67,772,818 (GRCm38)	missense	probably damaging	0.97
R0941:Lama1	UTSW	17	67,775,865 (GRCm38)	missense	probably benign	0.10
R1025:Lama1	UTSW	17	67,752,898 (GRCm38)	missense	probably benign	0.00
R1084:Lama1	UTSW	17	67,804,469 (GRCm38)	missense	probably benign	0.12
R1103:Lama1	UTSW	17	67,790,947 (GRCm38)	missense	probably damaging	0.98
R1420:Lama1	UTSW	17	67,790,947 (GRCm38)	missense	probably damaging	0.98
R1430:Lama1	UTSW	17	67,782,155 (GRCm38)	missense	possibly damaging	0.95
R1569:Lama1	UTSW	17	67,780,618 (GRCm38)	splice site	probably null
R1575:Lama1	UTSW	17	67,810,409 (GRCm38)	missense	possibly damaging	0.96
R1613:Lama1	UTSW	17	67,807,923 (GRCm38)	missense	probably benign	0.42
R1620:Lama1	UTSW	17	67,767,033 (GRCm38)	missense	probably benign	0.01
R1629:Lama1	UTSW	17	67,805,428 (GRCm38)	missense	probably benign	0.00
R1645:Lama1	UTSW	17	67,737,682 (GRCm38)	missense	probably benign	0.14
R1652:Lama1	UTSW	17	67,807,846 (GRCm38)	missense	probably damaging	0.97
R1674:Lama1	UTSW	17	67,791,244 (GRCm38)	missense	probably benign
R1678:Lama1	UTSW	17	67,810,155 (GRCm38)	missense	possibly damaging	0.56
R1710:Lama1	UTSW	17	67,753,791 (GRCm38)	missense	probably benign	0.00
R1712:Lama1	UTSW	17	67,717,186 (GRCm38)	missense	possibly damaging	0.95
R1737:Lama1	UTSW	17	67,802,921 (GRCm38)	missense	probably benign	0.36
R1757:Lama1	UTSW	17	67,697,383 (GRCm38)	missense	unknown
R1757:Lama1	UTSW	17	67,763,836 (GRCm38)	missense	probably benign	0.40
R1813:Lama1	UTSW	17	67,791,223 (GRCm38)	missense	probably benign
R1896:Lama1	UTSW	17	67,791,223 (GRCm38)	missense	probably benign
R1945:Lama1	UTSW	17	67,745,853 (GRCm38)	missense	probably benign	0.14
R2086:Lama1	UTSW	17	67,817,623 (GRCm38)	missense	probably damaging	1.00
R2149:Lama1	UTSW	17	67,773,865 (GRCm38)	missense	possibly damaging	0.95
R2178:Lama1	UTSW	17	67,769,515 (GRCm38)	missense	probably benign	0.07
R2183:Lama1	UTSW	17	67,791,009 (GRCm38)	missense	probably damaging	0.98
R2197:Lama1	UTSW	17	67,752,941 (GRCm38)	missense	probably benign	0.02
R2213:Lama1	UTSW	17	67,777,034 (GRCm38)	nonsense	probably null
R2260:Lama1	UTSW	17	67,737,507 (GRCm38)	missense	probably damaging	0.96
R2356:Lama1	UTSW	17	67,810,114 (GRCm38)	missense	probably damaging	1.00
R2420:Lama1	UTSW	17	67,750,553 (GRCm38)	missense	probably benign	0.00
R2421:Lama1	UTSW	17	67,750,553 (GRCm38)	missense	probably benign	0.00
R2422:Lama1	UTSW	17	67,750,553 (GRCm38)	missense	probably benign	0.00
R2424:Lama1	UTSW	17	67,798,665 (GRCm38)	missense	probably benign	0.09
R2442:Lama1	UTSW	17	67,768,317 (GRCm38)	missense	probably benign	0.04
R3147:Lama1	UTSW	17	67,737,658 (GRCm38)	missense	probably damaging	0.98
R3414:Lama1	UTSW	17	67,737,603 (GRCm38)	missense	probably damaging	1.00
R3683:Lama1	UTSW	17	67,768,333 (GRCm38)	missense	probably benign	0.40
R3820:Lama1	UTSW	17	67,779,046 (GRCm38)	splice site	probably null
R3821:Lama1	UTSW	17	67,779,046 (GRCm38)	splice site	probably null
R3822:Lama1	UTSW	17	67,779,046 (GRCm38)	splice site	probably null
R4012:Lama1	UTSW	17	67,812,373 (GRCm38)	nonsense	probably null
R4113:Lama1	UTSW	17	67,764,703 (GRCm38)	missense	probably benign	0.01
R4133:Lama1	UTSW	17	67,812,486 (GRCm38)	missense	probably damaging	1.00
R4133:Lama1	UTSW	17	67,750,655 (GRCm38)	missense	probably damaging	0.98
R4259:Lama1	UTSW	17	67,752,418 (GRCm38)	missense	possibly damaging	0.95
R4278:Lama1	UTSW	17	67,791,517 (GRCm38)	missense	probably null	0.00
R4321:Lama1	UTSW	17	67,771,083 (GRCm38)	missense	probably benign	0.03
R4374:Lama1	UTSW	17	67,804,518 (GRCm38)	missense	probably benign	0.00
R4386:Lama1	UTSW	17	67,773,712 (GRCm38)	missense	probably benign	0.01
R4463:Lama1	UTSW	17	67,761,700 (GRCm38)	missense	probably damaging	1.00
R4629:Lama1	UTSW	17	67,805,360 (GRCm38)	critical splice acceptor site	probably null
R4630:Lama1	UTSW	17	67,794,300 (GRCm38)	missense	probably benign	0.00
R4633:Lama1	UTSW	17	67,798,584 (GRCm38)	missense	probably damaging	0.96
R4668:Lama1	UTSW	17	67,752,434 (GRCm38)	missense	probably benign	0.27
R4684:Lama1	UTSW	17	67,773,778 (GRCm38)	missense	possibly damaging	0.88
R4745:Lama1	UTSW	17	67,738,780 (GRCm38)	missense	probably damaging	1.00
R4786:Lama1	UTSW	17	67,773,859 (GRCm38)	missense	possibly damaging	0.77
R4797:Lama1	UTSW	17	67,716,775 (GRCm38)	missense	probably benign	0.04
R4803:Lama1	UTSW	17	67,809,271 (GRCm38)	missense	probably damaging	1.00
R4925:Lama1	UTSW	17	67,794,314 (GRCm38)	missense	probably benign	0.02
R4939:Lama1	UTSW	17	67,737,475 (GRCm38)	missense	possibly damaging	0.91
R4952:Lama1	UTSW	17	67,767,566 (GRCm38)	critical splice donor site	probably null
R4975:Lama1	UTSW	17	67,738,834 (GRCm38)	missense	possibly damaging	0.95
R4977:Lama1	UTSW	17	67,737,682 (GRCm38)	missense	probably damaging	1.00
R5039:Lama1	UTSW	17	67,745,893 (GRCm38)	missense	possibly damaging	0.66
R5047:Lama1	UTSW	17	67,743,281 (GRCm38)	nonsense	probably null
R5195:Lama1	UTSW	17	67,764,800 (GRCm38)	missense	probably benign	0.13
R5230:Lama1	UTSW	17	67,745,083 (GRCm38)	nonsense	probably null
R5236:Lama1	UTSW	17	67,804,492 (GRCm38)	missense	probably benign	0.24
R5254:Lama1	UTSW	17	67,756,716 (GRCm38)	missense	probably benign	0.01
R5345:Lama1	UTSW	17	67,817,563 (GRCm38)	missense	probably benign
R5438:Lama1	UTSW	17	67,800,774 (GRCm38)	missense	possibly damaging	0.92
R5521:Lama1	UTSW	17	67,780,894 (GRCm38)	nonsense	probably null
R5568:Lama1	UTSW	17	67,768,298 (GRCm38)	critical splice acceptor site	probably null
R5645:Lama1	UTSW	17	67,802,948 (GRCm38)	missense	probably damaging	1.00
R5665:Lama1	UTSW	17	67,770,987 (GRCm38)	missense	probably damaging	1.00
R5727:Lama1	UTSW	17	67,815,224 (GRCm38)	missense	possibly damaging	0.81
R5757:Lama1	UTSW	17	67,738,787 (GRCm38)	missense	possibly damaging	0.59
R5795:Lama1	UTSW	17	67,796,727 (GRCm38)	missense	probably benign	0.02
R5857:Lama1	UTSW	17	67,807,843 (GRCm38)	missense	probably damaging	0.99
R5894:Lama1	UTSW	17	67,779,047 (GRCm38)	critical splice acceptor site	probably null
R5974:Lama1	UTSW	17	67,773,727 (GRCm38)	missense	probably benign	0.31
R6032:Lama1	UTSW	17	67,750,643 (GRCm38)	missense	probably benign	0.01
R6032:Lama1	UTSW	17	67,750,643 (GRCm38)	missense	probably benign	0.01
R6120:Lama1	UTSW	17	67,780,617 (GRCm38)	critical splice donor site	probably null
R6219:Lama1	UTSW	17	67,790,856 (GRCm38)	missense	probably benign	0.08
R6224:Lama1	UTSW	17	67,802,987 (GRCm38)	missense	possibly damaging	0.56
R6249:Lama1	UTSW	17	67,798,604 (GRCm38)	missense	probably benign
R6265:Lama1	UTSW	17	67,750,655 (GRCm38)	missense	probably damaging	0.98
R6276:Lama1	UTSW	17	67,784,088 (GRCm38)	splice site	probably null
R6284:Lama1	UTSW	17	67,810,096 (GRCm38)	missense	probably damaging	0.99
R6337:Lama1	UTSW	17	67,786,019 (GRCm38)	missense	probably benign	0.27
R6414:Lama1	UTSW	17	67,746,910 (GRCm38)	critical splice donor site	probably null
R6631:Lama1	UTSW	17	67,774,482 (GRCm38)	missense	probably benign	0.21
R6659:Lama1	UTSW	17	67,818,635 (GRCm38)	missense	probably damaging	1.00
R6660:Lama1	UTSW	17	67,804,500 (GRCm38)	missense	probably benign	0.05
R6677:Lama1	UTSW	17	67,795,233 (GRCm38)	missense	probably benign	0.14
R6763:Lama1	UTSW	17	67,746,873 (GRCm38)	missense	unknown
R6787:Lama1	UTSW	17	67,784,025 (GRCm38)	missense	unknown
R6831:Lama1	UTSW	17	67,756,754 (GRCm38)	missense	possibly damaging	0.89
R6910:Lama1	UTSW	17	67,791,464 (GRCm38)	missense	possibly damaging	0.60
R6934:Lama1	UTSW	17	67,774,543 (GRCm38)	missense	probably benign	0.04
R6945:Lama1	UTSW	17	67,813,866 (GRCm38)	missense
R6984:Lama1	UTSW	17	67,779,112 (GRCm38)	missense
R6989:Lama1	UTSW	17	67,753,758 (GRCm38)	missense
R6994:Lama1	UTSW	17	67,753,825 (GRCm38)	missense
R6995:Lama1	UTSW	17	67,753,825 (GRCm38)	missense
R7035:Lama1	UTSW	17	67,781,049 (GRCm38)	missense
R7133:Lama1	UTSW	17	67,782,146 (GRCm38)	missense
R7172:Lama1	UTSW	17	67,804,545 (GRCm38)	missense
R7197:Lama1	UTSW	17	67,737,705 (GRCm38)	nonsense	probably null
R7217:Lama1	UTSW	17	67,764,673 (GRCm38)	missense
R7229:Lama1	UTSW	17	67,752,446 (GRCm38)	missense
R7264:Lama1	UTSW	17	67,743,297 (GRCm38)	missense
R7311:Lama1	UTSW	17	67,767,385 (GRCm38)	missense
R7394:Lama1	UTSW	17	67,717,261 (GRCm38)	missense
R7419:Lama1	UTSW	17	67,717,174 (GRCm38)	missense
R7460:Lama1	UTSW	17	67,767,018 (GRCm38)	missense
R7492:Lama1	UTSW	17	67,817,651 (GRCm38)	missense
R7494:Lama1	UTSW	17	67,811,446 (GRCm38)	missense
R7552:Lama1	UTSW	17	67,737,667 (GRCm38)	missense
R7576:Lama1	UTSW	17	67,782,041 (GRCm38)	missense
R7583:Lama1	UTSW	17	67,761,621 (GRCm38)	missense
R7649:Lama1	UTSW	17	67,737,554 (GRCm38)	missense
R7663:Lama1	UTSW	17	67,780,880 (GRCm38)	missense
R7667:Lama1	UTSW	17	67,780,597 (GRCm38)	missense
R7688:Lama1	UTSW	17	67,761,628 (GRCm38)	missense
R7693:Lama1	UTSW	17	67,817,031 (GRCm38)	missense
R7748:Lama1	UTSW	17	67,750,590 (GRCm38)	missense
R7778:Lama1	UTSW	17	67,804,473 (GRCm38)	missense
R7824:Lama1	UTSW	17	67,804,473 (GRCm38)	missense
R7861:Lama1	UTSW	17	67,809,221 (GRCm38)	missense
R7884:Lama1	UTSW	17	67,769,435 (GRCm38)	missense
R8029:Lama1	UTSW	17	67,817,594 (GRCm38)	missense
R8078:Lama1	UTSW	17	67,791,294 (GRCm38)	missense
R8101:Lama1	UTSW	17	67,745,922 (GRCm38)	missense
R8313:Lama1	UTSW	17	67,750,520 (GRCm38)	missense
R8356:Lama1	UTSW	17	67,737,496 (GRCm38)	missense
R8366:Lama1	UTSW	17	67,818,704 (GRCm38)	missense
R8403:Lama1	UTSW	17	67,745,923 (GRCm38)	missense
R8456:Lama1	UTSW	17	67,737,496 (GRCm38)	missense
R8466:Lama1	UTSW	17	67,813,953 (GRCm38)	missense
R8678:Lama1	UTSW	17	67,817,103 (GRCm38)	missense
R8728:Lama1	UTSW	17	67,818,668 (GRCm38)	missense
R8796:Lama1	UTSW	17	67,810,151 (GRCm38)	missense
R8885:Lama1	UTSW	17	67,773,784 (GRCm38)	missense
R8893:Lama1	UTSW	17	67,805,372 (GRCm38)	missense
R8898:Lama1	UTSW	17	67,821,615 (GRCm38)	missense
R8909:Lama1	UTSW	17	67,772,741 (GRCm38)	missense
R9025:Lama1	UTSW	17	67,812,496 (GRCm38)	missense
R9045:Lama1	UTSW	17	67,753,843 (GRCm38)	missense
R9098:Lama1	UTSW	17	67,804,513 (GRCm38)	missense
R9114:Lama1	UTSW	17	67,821,674 (GRCm38)	missense
R9173:Lama1	UTSW	17	67,769,602 (GRCm38)	missense
R9190:Lama1	UTSW	17	67,804,519 (GRCm38)	missense
R9381:Lama1	UTSW	17	67,737,484 (GRCm38)	missense
R9429:Lama1	UTSW	17	67,811,454 (GRCm38)	missense
R9504:Lama1	UTSW	17	67,821,666 (GRCm38)	missense
R9558:Lama1	UTSW	17	67,817,009 (GRCm38)	missense
R9647:Lama1	UTSW	17	67,717,175 (GRCm38)	missense
R9651:Lama1	UTSW	17	67,794,220 (GRCm38)	missense
R9654:Lama1	UTSW	17	67,794,271 (GRCm38)	missense
R9710:Lama1	UTSW	17	67,822,409 (GRCm38)	missense
R9733:Lama1	UTSW	17	67,809,945 (GRCm38)	missense
RF001:Lama1	UTSW	17	67,752,902 (GRCm38)	missense
RF013:Lama1	UTSW	17	67,781,062 (GRCm38)	missense
V8831:Lama1	UTSW	17	67,752,883 (GRCm38)	missense	probably benign	0.00
X0024:Lama1	UTSW	17	67,738,888 (GRCm38)	missense	probably damaging	1.00
X0028:Lama1	UTSW	17	67,794,310 (GRCm38)	missense	probably benign	0.06
X0028:Lama1	UTSW	17	67,767,422 (GRCm38)	missense	probably benign	0.00
X0066:Lama1	UTSW	17	67,811,566 (GRCm38)	missense	probably damaging	1.00
Z1088:Lama1	UTSW	17	67,810,171 (GRCm38)	missense	probably damaging	1.00
Z1088:Lama1	UTSW	17	67,771,082 (GRCm38)	missense	probably benign	0.25
Z1088:Lama1	UTSW	17	67,752,883 (GRCm38)	missense	probably benign	0.00
Z1176:Lama1	UTSW	17	67,752,883 (GRCm38)	missense	probably benign	0.00
Z1177:Lama1	UTSW	17	67,752,883 (GRCm38)	missense	probably benign	0.00
Z1191:Lama1	UTSW	17	67,798,644 (GRCm38)	missense

Predicted Primers

PCR Primer
(F):5'- TTCTCCTTCCAATAAATACCATGGC -3'
(R):5'- AGGTTTCTGAGAAGCAACTCC -3'

Sequencing Primer
(F):5'- CTTGTGTAGCTCAGGGATAAAAGCC -3'
(R):5'- GGTTTCTGAGAAGCAACTCCATTAG -3'

Posted On

2018-09-12