Mutagenetix > Incidental Mutation

Incidental Mutation 'R6856:Fam198b'

535239

Institutional Source

Beutler Lab

Gene Symbol

Fam198b

Ensembl Gene

ENSMUSG00000027955

Gene Name

family with sequence similarity 198, member B

Synonyms

2210419I08Rik, 1110032E23Rik, Ened

MMRRC Submission

044958-MU

Accession Numbers

NCBI RefSeq: NM_133187.3; MGI:1915909

Essential gene?

Probably non essential (E-score: 0.062) question?

Stock #

R6856 (G1)

Quality Score

213.009

Status

Not validated

Chromosome

Chromosomal Location

79884533-79946280 bp(+) (GRCm38)

Type of Mutation

intron

DNA Base Change (assembly)

G to T at 79886141 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000120603 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029567] [ENSMUST00000118853] [ENSMUST00000135021] [ENSMUST00000145992]

AlphaFold

Q3UPI1

Predicted Effect

probably benign
Transcript: ENSMUST00000029567

SMART Domains

Protein: ENSMUSP00000029567
Gene: ENSMUSG00000027955

Domain	Start	End	E-Value	Type
transmembrane domain	37	56	N/A	INTRINSIC
Pfam:FAM198	202	516	9.1e-156	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000118853

SMART Domains

Protein: ENSMUSP00000114093
Gene: ENSMUSG00000027955

Domain	Start	End	E-Value	Type
transmembrane domain	37	56	N/A	INTRINSIC
Pfam:FAM198	202	516	1.1e-155	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000135021

Predicted Effect

probably benign
Transcript: ENSMUST00000145992

SMART Domains

Protein: ENSMUSP00000120603
Gene: ENSMUSG00000027955

Domain	Start	End	E-Value	Type
Pfam:FAM198	1	51	5.4e-27	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

Allele List at MGI

All alleles(2) : Targeted(2)

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrl4	A	G	3: 151,500,118 (GRCm38)	M156V	probably benign	Het
Aire	A	T	10: 78,030,255 (GRCm38)	F546I	probably damaging	Het
Ankk1	T	C	9: 49,420,020 (GRCm38)	E230G	probably benign	Het
Anp32a	A	T	9: 62,372,115 (GRCm38)	K86N	possibly damaging	Het
Aqp4	T	C	18: 15,399,896 (GRCm38)	I47V	possibly damaging	Het
Arap3	A	G	18: 37,979,863 (GRCm38)	V1098A	possibly damaging	Het
Ascc3	T	A	10: 50,749,062 (GRCm38)	W1652R	probably damaging	Het
Atad2	T	A	15: 58,106,813 (GRCm38)	H464L	probably damaging	Het
Brca2	C	A	5: 150,540,208 (GRCm38)	H1146N	possibly damaging	Het
Capn9	G	A	8: 124,597,569 (GRCm38)	V203M	probably damaging	Het
Ccr6	T	A	17: 8,256,049 (GRCm38)	S29T	probably benign	Het
Cfap99	G	T	5: 34,310,217 (GRCm38)		probably null	Het
Cpt1c	C	T	7: 44,959,918 (GRCm38)	G716S	probably damaging	Het
Dhx29	A	T	13: 112,952,861 (GRCm38)	Q722L	probably benign	Het
Dmxl1	C	T	18: 49,852,288 (GRCm38)	R201*	probably null	Het
Dsg2	G	A	18: 20,601,802 (GRCm38)	G946S	probably damaging	Het
Erg	C	A	16: 95,368,651 (GRCm38)		probably null	Het
Fbxo32	G	A	15: 58,214,641 (GRCm38)		probably benign	Het
Glis1	T	G	4: 107,435,879 (GRCm38)	D66E	probably damaging	Het
Gm21671	A	G	5: 25,950,845 (GRCm38)	I167T	probably benign	Het
Grm6	A	T	11: 50,859,825 (GRCm38)	N605I	probably damaging	Het
Gtf3c6	T	C	10: 40,249,672 (GRCm38)	E183G	probably benign	Het
Herc1	A	G	9: 66,397,898 (GRCm38)	M861V	probably benign	Het
Igkv12-41	A	T	6: 69,858,529 (GRCm38)	S80T	probably damaging	Het
Kcnt2	T	C	1: 140,596,004 (GRCm38)	S1057P	probably damaging	Het
Krt36	T	G	11: 100,103,390 (GRCm38)	Q287P	probably damaging	Het
Ldhd	A	G	8: 111,630,274 (GRCm38)	S13P	probably benign	Het
Lmtk3	T	A	7: 45,794,297 (GRCm38)		probably benign	Het
Lrp2	A	T	2: 69,513,268 (GRCm38)	F916I	probably damaging	Het
Map4k1	A	T	7: 28,986,834 (GRCm38)	I92F	probably damaging	Het
Naa25	A	T	5: 121,438,804 (GRCm38)	K872M	probably damaging	Het
Nek3	C	A	8: 22,129,447 (GRCm38)	G443V	probably damaging	Het
Noxred1	T	C	12: 87,227,036 (GRCm38)	E77G	probably benign	Het
Nup210	G	A	6: 91,087,913 (GRCm38)	Q202*	probably null	Het
Olfr1065	G	T	2: 86,445,907 (GRCm38)	S25Y	probably benign	Het
Olfr646	A	T	7: 104,106,791 (GRCm38)	M171L	probably benign	Het
Pax3	A	G	1: 78,132,419 (GRCm38)	S201P	probably damaging	Het
Pcdhgb5	T	A	18: 37,733,404 (GRCm38)	Y751N	probably benign	Het
Pign	A	T	1: 105,553,895 (GRCm38)	L792*	probably null	Het
Pkd1	T	C	17: 24,573,493 (GRCm38)	F1385L	probably benign	Het
Plxnb2	A	C	15: 89,164,320 (GRCm38)	C629G	probably benign	Het
Prpsap2	A	T	11: 61,730,271 (GRCm38)	I328N	probably benign	Het
Prrc2c	A	G	1: 162,682,371 (GRCm38)	L2317P	probably damaging	Het
Ptgfrn	T	C	3: 101,045,446 (GRCm38)	D824G	probably damaging	Het
Ptpra	G	A	2: 130,519,381 (GRCm38)	S204N	probably damaging	Het
Pygm	G	T	19: 6,393,757 (GRCm38)	G583C	probably damaging	Het
Rap1a	A	G	3: 105,732,068 (GRCm38)	F92L	probably damaging	Het
Slmap	A	T	14: 26,430,092 (GRCm38)		probably null	Het
Spdye4c	A	G	2: 128,596,130 (GRCm38)		probably null	Het
Ssfa2	C	T	2: 79,657,705 (GRCm38)	R711C	probably damaging	Het
Stk11	C	A	10: 80,128,090 (GRCm38)	F97L	probably benign	Het
Tbc1d9b	G	A	11: 50,168,746 (GRCm38)	A992T	probably benign	Het
Tmem232	G	A	17: 65,450,310 (GRCm38)	T296M	possibly damaging	Het
Trim33	C	T	3: 103,352,049 (GRCm38)	T1018M	probably damaging	Het
Trpv2	A	C	11: 62,584,615 (GRCm38)	I285L	probably benign	Het
Usp46	A	G	5: 74,028,934 (GRCm38)		probably benign	Het
Vmn1r27	T	A	6: 58,215,447 (GRCm38)	M191L	possibly damaging	Het
Vwf	G	T	6: 125,642,150 (GRCm38)	E1264*	probably null	Het
Zfp109	A	T	7: 24,229,398 (GRCm38)	N195K	probably benign	Het
Zfp385b	T	A	2: 77,415,794 (GRCm38)	L208F	probably damaging	Het
Zfp839	T	A	12: 110,866,761 (GRCm38)	Y515*	probably null	Het

Other mutations in Fam198b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02952:Fam198b	APN	3	79,886,339 (GRCm38)	missense	probably damaging	1.00
P0015:Fam198b	UTSW	3	79,936,608 (GRCm38)	missense	probably damaging	1.00
PIT4362001:Fam198b	UTSW	3	79,886,939 (GRCm38)	missense	possibly damaging	0.78
R1519:Fam198b	UTSW	3	79,941,464 (GRCm38)	missense	possibly damaging	0.88
R1723:Fam198b	UTSW	3	79,936,663 (GRCm38)	missense	probably benign
R1782:Fam198b	UTSW	3	79,886,531 (GRCm38)	missense	possibly damaging	0.85
R3040:Fam198b	UTSW	3	79,887,125 (GRCm38)	missense	possibly damaging	0.95
R3840:Fam198b	UTSW	3	79,908,590 (GRCm38)	missense	probably benign	0.32
R4841:Fam198b	UTSW	3	79,936,605 (GRCm38)	missense	probably damaging	1.00
R4842:Fam198b	UTSW	3	79,936,605 (GRCm38)	missense	probably damaging	1.00
R4860:Fam198b	UTSW	3	79,936,674 (GRCm38)	nonsense	probably null
R4860:Fam198b	UTSW	3	79,936,674 (GRCm38)	nonsense	probably null
R5181:Fam198b	UTSW	3	79,886,311 (GRCm38)	missense	probably benign	0.08
R5266:Fam198b	UTSW	3	79,936,603 (GRCm38)	missense	probably damaging	0.96
R6353:Fam198b	UTSW	3	79,941,340 (GRCm38)	missense	probably damaging	1.00
R6698:Fam198b	UTSW	3	79,936,595 (GRCm38)	missense	probably damaging	0.97
R6927:Fam198b	UTSW	3	79,941,462 (GRCm38)	missense	probably damaging	1.00
R7025:Fam198b	UTSW	3	79,886,548 (GRCm38)	missense	probably damaging	1.00
R7189:Fam198b	UTSW	3	79,886,807 (GRCm38)	nonsense	probably null
R7434:Fam198b	UTSW	3	79,941,362 (GRCm38)	missense	probably damaging	1.00
R7557:Fam198b	UTSW	3	79,886,608 (GRCm38)	nonsense	probably null
R7780:Fam198b	UTSW	3	79,941,404 (GRCm38)	missense	probably damaging	1.00
R7891:Fam198b	UTSW	3	79,886,284 (GRCm38)	missense	probably benign	0.00
R8812:Fam198b	UTSW	3	79,908,771 (GRCm38)	missense	possibly damaging	0.82
R8841:Fam198b	UTSW	3	79,887,119 (GRCm38)	missense	probably benign	0.08
R8960:Fam198b	UTSW	3	79,886,986 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AAGGGTTACTCGCCTCTTTC -3'
(R):5'- ACTCCAAAGTTTACACACTCGTG -3'

Sequencing Primer
(F):5'- GTTGAGTTAGTGATTCAGTCAACTC -3'
(R):5'- TCGTGGGGCGCACAGAG -3'

Posted On

2018-09-12