Mutagenetix > Incidental Mutation

Incidental Mutation 'R6860:Slit1'

535484

Institutional Source

Beutler Lab

Gene Symbol

Slit1

Ensembl Gene

ENSMUSG00000025020

Gene Name

slit guidance ligand 1

Synonyms

Slil1

MMRRC Submission

045025-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6860 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

41588696-41732104 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 41605154 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 899 (M899T)

Ref Sequence

ENSEMBL: ENSMUSP00000128381 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025993] [ENSMUST00000166496] [ENSMUST00000169141]

AlphaFold

Q80TR4

Predicted Effect

probably benign
Transcript: ENSMUST00000025993
AA Change: M899T

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000025993
Gene: ENSMUSG00000025020
AA Change: M899T

Domain	Start	End	E-Value	Type
signal peptide	1	32	N/A	INTRINSIC
LRRNT	33	65	5.68e-9	SMART
LRR	59	83	4.58e1	SMART
LRR	84	107	4.7e0	SMART
LRR_TYP	108	131	1.95e-3	SMART
LRR	133	155	6.05e0	SMART
LRR	157	179	3.98e1	SMART
LRR_TYP	180	203	3.44e-4	SMART
LRRCT	215	264	3.51e-6	SMART
LRRNT	281	313	3e-8	SMART
LRR	307	331	6.41e1	SMART
LRR_TYP	332	355	8.22e-2	SMART
LRR_TYP	356	379	9.08e-4	SMART
LRR	380	403	2.82e0	SMART
LRR_TYP	404	427	5.42e-2	SMART
LRRCT	439	488	5.78e-7	SMART
LRRNT	512	544	1.04e-7	SMART
LRR_TYP	564	587	3.39e-3	SMART
LRR	589	611	2.08e1	SMART
LRR_TYP	612	635	1.56e-2	SMART
LRR_TYP	636	659	4.11e-2	SMART
LRRCT	671	720	2.89e-7	SMART
LRRNT	733	765	4.87e-8	SMART
LRR	783	806	1.22e1	SMART
LRR_TYP	807	830	9.73e-4	SMART
LRR_TYP	831	854	9.58e-3	SMART
LRRCT	866	915	5.6e-14	SMART
EGF	928	962	5.08e-7	SMART
EGF	967	1003	1.74e-5	SMART
EGF_CA	1005	1041	1.05e-8	SMART
EGF	1046	1081	1.21e-4	SMART
EGF_CA	1083	1119	3.64e-8	SMART
FOLN	1086	1108	8.44e0	SMART
FOLN	1127	1149	1.4e0	SMART
EGF	1127	1160	1.78e-2	SMART
LamG	1183	1319	4.43e-38	SMART
EGF	1338	1371	6.76e-3	SMART
EGF	1377	1410	3.38e-3	SMART
FOLN	1418	1440	2.25e1	SMART
EGF	1418	1451	1.28e-3	SMART
CT	1462	1531	3.15e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000166496
AA Change: M899T

PolyPhen 2 Score 0.101 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000128381
Gene: ENSMUSG00000025020
AA Change: M899T

Domain	Start	End	E-Value	Type
signal peptide	1	32	N/A	INTRINSIC
LRRNT	33	65	5.68e-9	SMART
LRR	59	83	4.58e1	SMART
LRR	84	107	4.7e0	SMART
LRR_TYP	108	131	1.95e-3	SMART
LRR	133	155	6.05e0	SMART
LRR	157	179	3.98e1	SMART
LRR_TYP	180	203	3.44e-4	SMART
LRRCT	215	264	3.51e-6	SMART
LRRNT	281	313	3e-8	SMART
LRR	307	331	6.41e1	SMART
LRR_TYP	332	355	8.22e-2	SMART
LRR_TYP	356	379	9.08e-4	SMART
LRR	380	403	2.82e0	SMART
LRR_TYP	404	427	5.42e-2	SMART
LRRCT	439	488	5.78e-7	SMART
LRRNT	512	544	1.04e-7	SMART
LRR_TYP	564	587	3.39e-3	SMART
LRR	589	611	2.08e1	SMART
LRR_TYP	612	635	1.56e-2	SMART
LRR_TYP	636	659	4.11e-2	SMART
LRRCT	671	720	2.89e-7	SMART
LRRNT	733	765	4.87e-8	SMART
LRR	783	806	1.22e1	SMART
LRR_TYP	807	830	9.73e-4	SMART
LRR_TYP	831	854	9.58e-3	SMART
LRRCT	866	915	5.6e-14	SMART
EGF	928	962	5.08e-7	SMART
EGF	967	1003	1.74e-5	SMART
EGF_CA	1005	1041	1.05e-8	SMART
EGF	1046	1081	1.21e-4	SMART
EGF_CA	1083	1119	3.64e-8	SMART
FOLN	1086	1108	8.44e0	SMART
FOLN	1127	1149	1.4e0	SMART
EGF	1127	1160	1.78e-2	SMART
LamG	1183	1319	4.43e-38	SMART
EGF	1338	1371	6.76e-3	SMART
EGF	1377	1410	3.38e-3	SMART
low complexity region	1437	1458	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000169141
AA Change: M899T

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000129034
Gene: ENSMUSG00000025020
AA Change: M899T

Domain	Start	End	E-Value	Type
signal peptide	1	32	N/A	INTRINSIC
LRRNT	33	65	5.68e-9	SMART
LRR	59	83	4.58e1	SMART
LRR	84	107	4.7e0	SMART
LRR_TYP	108	131	1.95e-3	SMART
LRR	133	155	6.05e0	SMART
LRR	157	179	3.98e1	SMART
LRR_TYP	180	203	3.44e-4	SMART
LRRCT	215	264	3.51e-6	SMART
LRRNT	281	313	3e-8	SMART
LRR	307	331	6.41e1	SMART
LRR_TYP	332	355	8.22e-2	SMART
LRR_TYP	356	379	9.08e-4	SMART
LRR	380	403	2.82e0	SMART
LRR_TYP	404	427	5.42e-2	SMART
LRRCT	439	488	5.78e-7	SMART
LRRNT	512	544	1.04e-7	SMART
LRR_TYP	564	587	3.39e-3	SMART
LRR	589	611	2.08e1	SMART
LRR_TYP	612	635	1.56e-2	SMART
LRR_TYP	636	659	4.11e-2	SMART
LRRCT	671	720	2.89e-7	SMART
LRRNT	733	765	4.87e-8	SMART
LRR	783	806	1.22e1	SMART
LRR_TYP	807	830	9.73e-4	SMART
LRR_TYP	831	854	9.58e-3	SMART
LRRCT	866	915	5.6e-14	SMART
EGF	928	962	5.08e-7	SMART
EGF	967	1003	1.74e-5	SMART
EGF_CA	1005	1041	1.05e-8	SMART
EGF	1046	1081	1.21e-4	SMART
EGF_CA	1083	1119	3.64e-8	SMART
FOLN	1086	1108	8.44e0	SMART
FOLN	1127	1149	1.4e0	SMART
EGF	1127	1160	1.78e-2	SMART
LamG	1183	1319	4.43e-38	SMART
EGF	1338	1371	6.76e-3	SMART
EGF	1377	1410	3.38e-3	SMART
FOLN	1418	1440	2.25e1	SMART
EGF	1418	1451	1.28e-3	SMART

Meta Mutation Damage Score

0.1771

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

100% (86/86)

MGI Phenotype

PHENOTYPE: Mice homozygous for a reporter allele exhibit normal interneuron numbers and morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	T	C	11: 72,080,412 (GRCm39)	E418G	probably damaging	Het
9930012K11Rik	C	A	14: 70,395,071 (GRCm39)	V28L	possibly damaging	Het
Abtb2	C	T	2: 103,539,770 (GRCm39)	R712*	probably null	Het
Acvr1c	C	T	2: 58,177,717 (GRCm39)	G171S	probably damaging	Het
Ahctf1	G	T	1: 179,580,853 (GRCm39)	A1783E	probably benign	Het
Anapc4	A	T	5: 53,006,170 (GRCm39)	Q149L	probably damaging	Het
Ankrd27	T	C	7: 35,327,952 (GRCm39)	V824A	possibly damaging	Het
Ankrd31	G	C	13: 96,968,094 (GRCm39)	C577S	probably benign	Het
Apol7c	T	C	15: 77,410,274 (GRCm39)	N224S	probably benign	Het
Arl14	A	T	3: 69,130,029 (GRCm39)	T59S	probably benign	Het
Astn1	T	A	1: 158,440,042 (GRCm39)	I870K	probably damaging	Het
B4galt1	A	T	4: 40,807,796 (GRCm39)	V335E	probably benign	Het
C2cd3	C	T	7: 100,039,448 (GRCm39)	P216S	probably benign	Het
Cchcr1	C	A	17: 35,840,015 (GRCm39)	N711K	possibly damaging	Het
Chd2	A	G	7: 73,147,558 (GRCm39)	F467L	possibly damaging	Het
Cntn6	A	T	6: 104,838,907 (GRCm39)	E987V	possibly damaging	Het
Col11a2	T	A	17: 34,272,572 (GRCm39)	L286H	probably damaging	Het
Cwc22	C	T	2: 77,759,792 (GRCm39)	R85Q	possibly damaging	Het
Cyp7a1	A	C	4: 6,272,587 (GRCm39)	F209V	probably damaging	Het
Dmtn	T	C	14: 70,852,322 (GRCm39)	T189A	possibly damaging	Het
Dnhd1	C	G	7: 105,327,473 (GRCm39)	N777K	probably benign	Het
Dusp16	A	T	6: 134,702,842 (GRCm39)	C216*	probably null	Het
Fan1	T	A	7: 64,022,234 (GRCm39)	N340Y	probably damaging	Het
Fbn1	T	C	2: 125,170,078 (GRCm39)	N1993S	probably damaging	Het
Fbxw22	A	G	9: 109,213,030 (GRCm39)	S306P	probably benign	Het
Ferd3l	T	C	12: 33,978,651 (GRCm39)	S55P	probably benign	Het
Fpr-rs3	A	G	17: 20,844,560 (GRCm39)	S194P	possibly damaging	Het
Gm19965	G	A	1: 116,748,609 (GRCm39)	D97N	probably benign	Het
Gtpbp2	A	G	17: 46,478,914 (GRCm39)		probably benign	Het
H3c7	G	T	13: 23,728,760 (GRCm39)	V36L	probably benign	Het
Hcn3	A	C	3: 89,067,152 (GRCm39)	I72S	possibly damaging	Het
Hk3	T	A	13: 55,162,278 (GRCm39)	N109Y	probably damaging	Het
Iqub	T	C	6: 24,505,737 (GRCm39)	E57G	possibly damaging	Het
Kcnk3	T	A	5: 30,779,397 (GRCm39)	M149K	possibly damaging	Het
Kcp	C	T	6: 29,505,719 (GRCm39)	G51D	probably benign	Het
Kif26a	C	A	12: 112,113,263 (GRCm39)	A53D	probably damaging	Het
Lifr	T	A	15: 7,202,418 (GRCm39)	I353K	probably benign	Het
Llph	A	T	10: 120,067,189 (GRCm39)	N102I	probably damaging	Het
Lmo3	C	A	6: 138,393,566 (GRCm39)	R18L	possibly damaging	Het
Lrrc46	T	C	11: 96,926,371 (GRCm39)	E175G	probably benign	Het
Ltk	A	T	2: 119,585,075 (GRCm39)	C128*	probably null	Het
Map1b	T	C	13: 99,571,275 (GRCm39)	E482G	probably damaging	Het
Mapk8ip2	T	C	15: 89,344,655 (GRCm39)	V740A	probably damaging	Het
Miga2	T	C	2: 30,261,175 (GRCm39)	W157R	probably benign	Het
Mlip	A	G	9: 77,009,675 (GRCm39)	*837Q	probably null	Het
Mroh2a	G	T	1: 88,182,657 (GRCm39)	R1195L	possibly damaging	Het
Myl1	T	C	1: 66,984,217 (GRCm39)		probably benign	Het
Or1l4	T	C	2: 37,092,189 (GRCm39)	L312P	possibly damaging	Het
Or2a5	T	C	6: 42,873,750 (GRCm39)	Y122H	probably benign	Het
Or51ab3	A	G	7: 103,201,075 (GRCm39)	I28V	probably benign	Het
P3h3	A	T	6: 124,834,331 (GRCm39)	V107D	probably benign	Het
Papolb	A	T	5: 142,514,651 (GRCm39)	S331T	possibly damaging	Het
Pars2	T	G	4: 106,511,700 (GRCm39)	V494G	probably benign	Het
Pcdhb3	C	A	18: 37,434,763 (GRCm39)	T243K	probably benign	Het
Pde9a	T	A	17: 31,689,698 (GRCm39)	M415K	probably damaging	Het
Ppp1r9b	G	A	11: 94,882,974 (GRCm39)	A201T	probably benign	Het
Prl	A	G	13: 27,248,942 (GRCm39)	N197S	possibly damaging	Het
Prl2c1	T	A	13: 28,035,724 (GRCm39)	M32K	probably benign	Het
Prrt4	T	C	6: 29,170,737 (GRCm39)	S572G	possibly damaging	Het
Psd	T	A	19: 46,310,858 (GRCm39)	D397V	probably damaging	Het
Psme2b	A	T	11: 48,836,307 (GRCm39)	Y213*	probably null	Het
Ptcd3	T	A	6: 71,874,094 (GRCm39)		probably null	Het
Ptprk	T	G	10: 28,210,480 (GRCm39)	F167L	probably damaging	Het
Rfx7	T	C	9: 72,524,226 (GRCm39)	V472A	probably damaging	Het
Sanbr	A	G	11: 23,575,100 (GRCm39)	L58P	probably damaging	Het
Scnn1g	T	C	7: 121,339,576 (GRCm39)	L125S	probably damaging	Het
Sec16a	T	C	2: 26,320,124 (GRCm39)	Y1438C	probably damaging	Het
Serinc3	C	T	2: 163,476,366 (GRCm39)	S155N	probably benign	Het
Slc3a2	A	T	19: 8,690,996 (GRCm39)	V78E	probably damaging	Het
Slc44a4	T	A	17: 35,140,044 (GRCm39)	L23Q	probably damaging	Het
Slco5a1	C	T	1: 12,951,420 (GRCm39)		probably benign	Het
Sorl1	A	G	9: 41,933,688 (GRCm39)	I1094T	probably benign	Het
Spag9	T	A	11: 93,972,196 (GRCm39)	L258Q	probably benign	Het
Strip1	T	C	3: 107,526,252 (GRCm39)	E488G	possibly damaging	Het
Stx18	G	A	5: 38,262,235 (GRCm39)	D30N	possibly damaging	Het
Sync	T	C	4: 129,181,583 (GRCm39)		probably null	Het
Syncrip	A	G	9: 88,358,849 (GRCm39)	V220A	probably damaging	Het
Tagln2	T	C	1: 172,333,476 (GRCm39)	I110T	probably benign	Het
Taok1	T	C	11: 77,432,627 (GRCm39)	T729A	probably benign	Het
Tgfbrap1	C	T	1: 43,106,759 (GRCm39)	V75I	possibly damaging	Het
Tnfrsf21	A	G	17: 43,327,957 (GRCm39)	T24A	probably benign	Het
Tnxb	T	A	17: 34,932,131 (GRCm39)	D2221E	probably damaging	Het
Triml1	A	G	8: 43,583,603 (GRCm39)	S333P	probably damaging	Het
Trp53bp1	C	A	2: 121,029,594 (GRCm39)	R1862L	probably damaging	Het
Tsc22d1	T	G	14: 76,655,732 (GRCm39)	I737S	possibly damaging	Het
U2af2	A	T	7: 5,082,273 (GRCm39)	K462N	possibly damaging	Het
Ubap2	A	C	4: 41,233,631 (GRCm39)	N86K	probably damaging	Het
Uso1	A	G	5: 92,343,207 (GRCm39)	K764E	probably benign	Het
Vmn1r192	T	A	13: 22,372,122 (GRCm39)	M33L	probably benign	Het

Other mutations in Slit1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00162:Slit1	APN	19	41,639,274 (GRCm39)	missense	probably damaging	1.00
IGL00515:Slit1	APN	19	41,612,940 (GRCm39)	missense	probably damaging	0.97
IGL00909:Slit1	APN	19	41,590,694 (GRCm39)	missense	possibly damaging	0.89
IGL00953:Slit1	APN	19	41,590,739 (GRCm39)	missense	probably damaging	1.00
IGL01116:Slit1	APN	19	41,594,824 (GRCm39)	missense	possibly damaging	0.93
IGL01457:Slit1	APN	19	41,599,483 (GRCm39)	missense	probably damaging	1.00
IGL01688:Slit1	APN	19	41,717,545 (GRCm39)	missense	probably damaging	1.00
IGL01720:Slit1	APN	19	41,622,653 (GRCm39)	missense	probably benign	0.01
IGL01925:Slit1	APN	19	41,596,817 (GRCm39)	missense	probably damaging	0.98
IGL02008:Slit1	APN	19	41,634,579 (GRCm39)	missense	probably damaging	0.99
IGL02312:Slit1	APN	19	41,590,119 (GRCm39)	missense	possibly damaging	0.66
IGL02398:Slit1	APN	19	41,590,676 (GRCm39)	missense	probably damaging	1.00
IGL02542:Slit1	APN	19	41,615,687 (GRCm39)	missense	probably damaging	1.00
IGL02559:Slit1	APN	19	41,709,524 (GRCm39)	missense	probably benign	0.01
IGL02609:Slit1	APN	19	41,590,743 (GRCm39)	missense	probably damaging	0.99
IGL02623:Slit1	APN	19	41,640,122 (GRCm39)	missense	probably damaging	0.98
IGL02729:Slit1	APN	19	41,591,773 (GRCm39)	missense	probably damaging	1.00
IGL03230:Slit1	APN	19	41,717,524 (GRCm39)	missense	probably damaging	1.00
IGL03387:Slit1	APN	19	41,591,881 (GRCm39)	missense	possibly damaging	0.57
PIT4576001:Slit1	UTSW	19	41,612,988 (GRCm39)	missense	possibly damaging	0.52
R0366:Slit1	UTSW	19	41,599,470 (GRCm39)	missense	probably damaging	1.00
R0432:Slit1	UTSW	19	41,731,732 (GRCm39)	missense	probably damaging	0.98
R0496:Slit1	UTSW	19	41,596,750 (GRCm39)	splice site	probably benign
R0722:Slit1	UTSW	19	41,596,874 (GRCm39)	missense	probably damaging	1.00
R1468:Slit1	UTSW	19	41,596,823 (GRCm39)	missense	probably damaging	1.00
R1468:Slit1	UTSW	19	41,596,823 (GRCm39)	missense	probably damaging	1.00
R1488:Slit1	UTSW	19	41,596,824 (GRCm39)	missense	probably damaging	1.00
R1615:Slit1	UTSW	19	41,639,110 (GRCm39)	splice site	probably benign
R1694:Slit1	UTSW	19	41,626,031 (GRCm39)	missense	possibly damaging	0.69
R1762:Slit1	UTSW	19	41,591,774 (GRCm39)	missense	probably damaging	1.00
R1842:Slit1	UTSW	19	41,709,477 (GRCm39)	critical splice donor site	probably null
R1844:Slit1	UTSW	19	41,614,012 (GRCm39)	missense	probably damaging	1.00
R1940:Slit1	UTSW	19	41,619,215 (GRCm39)	missense	probably damaging	1.00
R2087:Slit1	UTSW	19	41,625,922 (GRCm39)	missense	probably benign	0.00
R2094:Slit1	UTSW	19	41,594,819 (GRCm39)	missense	probably damaging	0.99
R2095:Slit1	UTSW	19	41,594,819 (GRCm39)	missense	probably damaging	0.99
R2104:Slit1	UTSW	19	41,590,686 (GRCm39)	missense	possibly damaging	0.69
R2305:Slit1	UTSW	19	41,599,455 (GRCm39)	missense	probably benign	0.03
R2972:Slit1	UTSW	19	41,599,455 (GRCm39)	missense	probably benign	0.03
R2973:Slit1	UTSW	19	41,599,455 (GRCm39)	missense	probably benign	0.03
R2974:Slit1	UTSW	19	41,599,455 (GRCm39)	missense	probably benign	0.03
R3159:Slit1	UTSW	19	41,592,812 (GRCm39)	missense	probably benign
R3752:Slit1	UTSW	19	41,635,406 (GRCm39)	critical splice donor site	probably null
R4095:Slit1	UTSW	19	41,596,925 (GRCm39)	intron	probably benign
R4282:Slit1	UTSW	19	41,602,856 (GRCm39)	missense	probably benign
R4417:Slit1	UTSW	19	41,602,908 (GRCm39)	missense	probably damaging	1.00
R4607:Slit1	UTSW	19	41,605,232 (GRCm39)	missense	probably benign	0.10
R4729:Slit1	UTSW	19	41,635,459 (GRCm39)	missense	probably damaging	1.00
R4756:Slit1	UTSW	19	41,637,452 (GRCm39)	missense	probably damaging	1.00
R4764:Slit1	UTSW	19	41,709,483 (GRCm39)	nonsense	probably null
R4849:Slit1	UTSW	19	41,637,983 (GRCm39)	missense	probably benign	0.17
R4874:Slit1	UTSW	19	41,717,493 (GRCm39)	critical splice donor site	probably null
R5581:Slit1	UTSW	19	41,605,102 (GRCm39)	critical splice donor site	probably null
R5699:Slit1	UTSW	19	41,613,959 (GRCm39)	critical splice donor site	probably null
R5888:Slit1	UTSW	19	41,731,735 (GRCm39)	missense	probably damaging	1.00
R5906:Slit1	UTSW	19	41,594,813 (GRCm39)	missense	probably damaging	1.00
R6176:Slit1	UTSW	19	41,626,034 (GRCm39)	missense	probably damaging	1.00
R6277:Slit1	UTSW	19	41,588,948 (GRCm39)	missense	possibly damaging	0.81
R6702:Slit1	UTSW	19	41,603,309 (GRCm39)	missense	possibly damaging	0.95
R7015:Slit1	UTSW	19	41,618,325 (GRCm39)	nonsense	probably null
R7172:Slit1	UTSW	19	41,623,105 (GRCm39)	missense	probably damaging	1.00
R7512:Slit1	UTSW	19	41,589,074 (GRCm39)	missense	probably damaging	1.00
R7568:Slit1	UTSW	19	41,590,074 (GRCm39)	missense	probably damaging	1.00
R7614:Slit1	UTSW	19	41,622,639 (GRCm39)	missense	probably damaging	1.00
R7650:Slit1	UTSW	19	41,618,363 (GRCm39)	missense	probably damaging	1.00
R7687:Slit1	UTSW	19	41,639,128 (GRCm39)	missense	probably benign	0.03
R7732:Slit1	UTSW	19	41,592,847 (GRCm39)	missense	probably benign	0.01
R7947:Slit1	UTSW	19	41,599,248 (GRCm39)	missense	probably benign
R7947:Slit1	UTSW	19	41,599,247 (GRCm39)	missense	probably damaging	1.00
R8171:Slit1	UTSW	19	41,715,512 (GRCm39)	missense	probably damaging	0.97
R8217:Slit1	UTSW	19	41,612,959 (GRCm39)	missense	possibly damaging	0.60
R8355:Slit1	UTSW	19	41,634,473 (GRCm39)	missense	probably damaging	1.00
R9025:Slit1	UTSW	19	41,612,968 (GRCm39)	missense	probably benign	0.01
R9124:Slit1	UTSW	19	41,594,951 (GRCm39)	missense	probably benign	0.02
R9288:Slit1	UTSW	19	41,613,144 (GRCm39)	intron	probably benign
R9343:Slit1	UTSW	19	41,615,737 (GRCm39)	missense	probably damaging	1.00
R9435:Slit1	UTSW	19	41,591,764 (GRCm39)	critical splice donor site	probably null
R9563:Slit1	UTSW	19	41,596,874 (GRCm39)	missense	probably damaging	1.00
R9564:Slit1	UTSW	19	41,591,861 (GRCm39)	missense	probably benign	0.16
R9595:Slit1	UTSW	19	41,637,851 (GRCm39)	missense	probably damaging	1.00
R9667:Slit1	UTSW	19	41,731,832 (GRCm39)	nonsense	probably null
X0023:Slit1	UTSW	19	41,590,079 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCCGCCTAGCTGTTTGTATG -3'
(R):5'- CCTCAGTGACATAAAAGCTTCCG -3'

Sequencing Primer
(F):5'- TGGAAGACAGCCATTCTAATCAG -3'
(R):5'- TTCCGAAACTGGAATGCCTG -3'

Posted On

2018-09-12