Mutagenetix > Incidental Mutation

Incidental Mutation 'R6850:Zfp35'

535621

Institutional Source

Beutler Lab

Gene Symbol

Zfp35

Ensembl Gene

ENSMUSG00000063281

Gene Name

zinc finger protein 35

Synonyms

Zfp-35

MMRRC Submission

044954-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.335) question?

Stock #

R6850 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

24122689-24138433 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 24135839 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 61 (R61H)

Ref Sequence

ENSEMBL: ENSMUSP00000074475 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074941]

AlphaFold

P15620

Predicted Effect

possibly damaging
Transcript: ENSMUST00000074941
AA Change: R61H

PolyPhen 2 Score 0.759 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000074475
Gene: ENSMUSG00000063281
AA Change: R61H

Domain	Start	End	E-Value	Type
ZnF_C2H2	78	100	1.28e-3	SMART
ZnF_C2H2	104	126	8.47e-4	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	1.84e-4	SMART
ZnF_C2H2	188	210	7.37e-4	SMART
ZnF_C2H2	216	238	4.72e-2	SMART
ZnF_C2H2	244	266	2.4e-3	SMART
ZnF_C2H2	272	294	1.04e-3	SMART
ZnF_C2H2	300	322	3.69e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	1.5e-4	SMART
ZnF_C2H2	384	406	1.6e-4	SMART
ZnF_C2H2	412	434	3.89e-3	SMART
ZnF_C2H2	440	462	1.72e-4	SMART
ZnF_C2H2	468	490	2.71e-2	SMART
ZnF_C2H2	496	518	1.6e-4	SMART
ZnF_C2H2	524	545	2.17e1	SMART
ZnF_C2H2	551	573	1.6e-4	SMART

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

98% (54/55)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out mutation exhibit increased airway responsiveness to ovalbumin and methacholine. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgb	T	C	10: 10,270,318 (GRCm39)	M927V	probably benign	Het
Agr2	A	G	12: 36,045,558 (GRCm39)	I15V	probably benign	Het
Alpk1	A	G	3: 127,523,012 (GRCm39)	I10T	possibly damaging	Het
Art5	A	G	7: 101,747,302 (GRCm39)	V159A	possibly damaging	Het
Asz1	G	A	6: 18,108,942 (GRCm39)	R32W	probably benign	Het
Atp8b1	A	C	18: 64,689,923 (GRCm39)	M603R	possibly damaging	Het
Cacna1s	G	A	1: 136,020,432 (GRCm39)	R823Q	probably benign	Het
Car9	A	G	4: 43,507,321 (GRCm39)	E3G	probably damaging	Het
Cpne3	A	T	4: 19,535,231 (GRCm39)	I267N	possibly damaging	Het
Cyp2c65	T	A	19: 39,057,535 (GRCm39)	F57I	probably benign	Het
D430041D05Rik	T	C	2: 104,031,604 (GRCm39)	K980R	probably damaging	Het
Dnhd1	G	T	7: 105,369,137 (GRCm39)	G4303W	possibly damaging	Het
Dnmt3a	A	G	12: 3,947,600 (GRCm39)	N485D	probably benign	Het
Dusp4	A	T	8: 35,283,651 (GRCm39)	K166*	probably null	Het
Ect2	T	C	3: 27,193,034 (GRCm39)	D344G	probably damaging	Het
Eif2b1	T	C	5: 124,717,069 (GRCm39)	D3G	probably benign	Het
Ermp1	A	G	19: 29,594,041 (GRCm39)	Y710H	probably damaging	Het
Gar1	T	C	3: 129,623,038 (GRCm39)	N117S	probably damaging	Het
H2-T10	A	G	17: 36,430,152 (GRCm39)	L263P	probably damaging	Het
Iqcn	A	T	8: 71,163,425 (GRCm39)	N873Y	probably damaging	Het
Itga7	A	T	10: 128,781,385 (GRCm39)	I621F	probably damaging	Het
Kcna3	A	T	3: 106,944,475 (GRCm39)	D246V	probably damaging	Het
Kctd12	C	T	14: 103,219,414 (GRCm39)	G155S	probably benign	Het
Lrtm1	T	C	14: 28,749,407 (GRCm39)	V256A	probably benign	Het
Mcf2l	T	C	8: 13,059,476 (GRCm39)	F629L	possibly damaging	Het
Mphosph9	A	G	5: 124,399,019 (GRCm39)	F999L	probably damaging	Het
Mtres1	ACTGCACCACCT	ACT	10: 43,408,721 (GRCm39)		probably benign	Het
Obscn	C	T	11: 58,958,950 (GRCm39)	V3643M	possibly damaging	Het
Obscn	C	T	11: 58,892,955 (GRCm39)	A6764T	possibly damaging	Het
Or4c102	T	A	2: 88,422,650 (GRCm39)	C167*	probably null	Het
Or8g18	T	C	9: 39,149,271 (GRCm39)	I150V	probably benign	Het
Pdhx	T	C	2: 102,871,445 (GRCm39)	H195R	probably damaging	Het
Pdilt	A	T	7: 119,086,182 (GRCm39)	V511E	probably damaging	Het
Prima1	C	T	12: 103,163,594 (GRCm39)	D126N	probably benign	Het
Prrc2c	TTGCTGCTGCTGCTGCTGCTGCTGCTGC	TTGCTGCTGCTGCTGCTGCTGCTGC	1: 162,536,630 (GRCm39)		probably benign	Het
Prune2	C	A	19: 17,099,552 (GRCm39)	D1685E	probably benign	Het
Ptgs2	A	T	1: 149,981,291 (GRCm39)	I525F	probably damaging	Het
Rab11fip2	A	T	19: 59,925,441 (GRCm39)	F259I	possibly damaging	Het
Ranbp3l	C	A	15: 9,058,808 (GRCm39)	D216E	probably damaging	Het
Scn5a	A	T	9: 119,330,815 (GRCm39)	L1240Q	possibly damaging	Het
Sohlh2	A	T	3: 55,099,707 (GRCm39)	T160S	probably benign	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Spata31h1	A	C	10: 82,128,888 (GRCm39)	M1374R	possibly damaging	Het
Tank	A	G	2: 61,480,346 (GRCm39)	E294G	probably benign	Het
Tars1	T	A	15: 11,392,885 (GRCm39)	Y187F	probably benign	Het
Tas2r144	T	A	6: 42,192,857 (GRCm39)	M199K	possibly damaging	Het
Tdrd3	T	C	14: 87,695,515 (GRCm39)		probably benign	Het
Tecta	T	A	9: 42,255,134 (GRCm39)	D1683V	probably benign	Het
Tln2	A	G	9: 67,165,817 (GRCm39)	I2098T	probably damaging	Het
Tmie	A	G	9: 110,695,980 (GRCm39)	I137T	possibly damaging	Het
Trim45	T	A	3: 100,830,541 (GRCm39)	L105*	probably null	Het
Trpv3	G	A	11: 73,182,519 (GRCm39)	G568S	probably damaging	Het
Wdr75	A	G	1: 45,853,758 (GRCm39)	T390A	probably benign	Het
Wdr97	A	G	15: 76,241,996 (GRCm39)	Y763C	probably damaging	Het

Other mutations in Zfp35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0012:Zfp35	UTSW	18	24,136,001 (GRCm39)	missense	probably benign	0.01
R1453:Zfp35	UTSW	18	24,136,557 (GRCm39)	nonsense	probably null
R1807:Zfp35	UTSW	18	24,136,986 (GRCm39)	missense	probably benign	0.28
R2317:Zfp35	UTSW	18	24,136,555 (GRCm39)	missense	probably damaging	1.00
R2863:Zfp35	UTSW	18	24,137,352 (GRCm39)	missense	probably damaging	0.99
R3080:Zfp35	UTSW	18	24,136,367 (GRCm39)	missense	probably damaging	0.99
R3756:Zfp35	UTSW	18	24,135,982 (GRCm39)	missense	possibly damaging	0.88
R4114:Zfp35	UTSW	18	24,135,766 (GRCm39)	missense	probably benign	0.08
R4222:Zfp35	UTSW	18	24,136,246 (GRCm39)	missense	possibly damaging	0.91
R4780:Zfp35	UTSW	18	24,136,326 (GRCm39)	missense	probably damaging	1.00
R5137:Zfp35	UTSW	18	24,137,194 (GRCm39)	nonsense	probably null
R5261:Zfp35	UTSW	18	24,136,778 (GRCm39)	missense	probably damaging	0.98
R5375:Zfp35	UTSW	18	24,135,973 (GRCm39)	missense	possibly damaging	0.83
R6001:Zfp35	UTSW	18	24,135,816 (GRCm39)	missense	probably benign
R6190:Zfp35	UTSW	18	24,137,118 (GRCm39)	missense	probably benign	0.00
R6774:Zfp35	UTSW	18	24,136,015 (GRCm39)	missense	possibly damaging	0.51
R6979:Zfp35	UTSW	18	24,136,927 (GRCm39)	missense	probably benign	0.32
R7029:Zfp35	UTSW	18	24,136,583 (GRCm39)	missense	probably damaging	0.98
R7079:Zfp35	UTSW	18	24,136,357 (GRCm39)	missense	possibly damaging	0.89
R7858:Zfp35	UTSW	18	24,136,897 (GRCm39)	missense	probably damaging	1.00
R8257:Zfp35	UTSW	18	24,137,288 (GRCm39)	missense	possibly damaging	0.55
R9477:Zfp35	UTSW	18	24,136,188 (GRCm39)	missense	possibly damaging	0.86
R9642:Zfp35	UTSW	18	24,137,155 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATACTCATTTGTTCTACCTGGAGC -3'
(R):5'- TTCTCTGATGCAGAACCAGGG -3'

Sequencing Primer
(F):5'- GAGCTTTCTTACAACATGGAGCGC -3'
(R):5'- AGAACCAGGGCTGAGCTCATC -3'

Posted On

2018-09-12