|Institutional Source||Beutler Lab|
|Gene Name||hyaluronoglucosaminidase 5|
|Is this an essential gene?||Probably non essential (E-score: 0.056)|
|Stock #||R6351 (G1)|
|Chromosomal Location||24857997-24891958 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||T to C at 24891709 bp|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000031689 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000031689]|
|Predicted Effect||probably null
|Meta Mutation Damage Score||0.9755|
|Coding Region Coverage||
|Validation Efficiency||99% (95/96)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
PHENOTYPE: Reproduction is normal in mice with null mutations at this marker. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Hyal5||
(F):5'- ACTGTCAGGAGAGAGCTGAC -3'
(R):5'- ACCCTGAAAGTATGACAGCCTTAC -3'
(F):5'- GCTGACATAGAAAGTGTTAGCAC -3'
(R):5'- GAAAGTATGACAGCCTTACAATCAG -3'