Mutagenetix > Incidental Mutation

Incidental Mutation 'R6884:Vmn2r73'

536840

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

044979-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.141) question?

Stock #

R6884 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

85506755-85525146 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 85507213 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 700 (T700S)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

probably benign
Transcript: ENSMUST00000077478
AA Change: T700S

PolyPhen 2 Score 0.054 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: T700S

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aamp	A	G	1: 74,323,407 (GRCm39)		probably benign	Het
Abca5	C	T	11: 110,220,043 (GRCm39)	V8M	probably damaging	Het
Ahnak2	A	T	12: 112,741,863 (GRCm39)	D736E	possibly damaging	Het
Brpf3	A	G	17: 29,050,324 (GRCm39)	D1072G	probably benign	Het
Cacna1i	C	T	15: 80,259,010 (GRCm39)	R1240C	probably damaging	Het
Ccdc40	A	T	11: 119,133,565 (GRCm39)	E568D	possibly damaging	Het
Col12a1	C	T	9: 79,547,091 (GRCm39)	G2247E	possibly damaging	Het
Dapk3	T	A	10: 81,027,588 (GRCm39)		probably null	Het
Dcaf13	T	C	15: 38,986,635 (GRCm39)	W136R	probably damaging	Het
Dip2a	C	A	10: 76,108,366 (GRCm39)		probably null	Het
Eif3c	T	C	7: 126,156,051 (GRCm39)	D473G	probably benign	Het
Erich2	G	A	2: 70,339,505 (GRCm39)	R20H	possibly damaging	Het
Gcnt7	T	C	2: 172,296,125 (GRCm39)	N233S	probably damaging	Het
Gdpd4	G	T	7: 97,621,382 (GRCm39)	L208F	probably damaging	Het
Gm10269	T	A	18: 20,815,932 (GRCm39)	Q30L	possibly damaging	Het
Gm7298	A	T	6: 121,737,480 (GRCm39)	I295F	possibly damaging	Het
Htr7	A	G	19: 35,941,779 (GRCm39)		probably null	Het
Ing1	A	G	8: 11,611,916 (GRCm39)	Y118C	probably damaging	Het
Krtap16-1	C	T	11: 99,877,284 (GRCm39)	W40*	probably null	Het
Krtap26-1	A	T	16: 88,444,467 (GRCm39)	D51E	probably damaging	Het
Lama5	G	A	2: 179,833,455 (GRCm39)	P1519L	probably damaging	Het
Lrp1	T	A	10: 127,394,986 (GRCm39)	Q2513L	probably benign	Het
Morf4l1	A	T	9: 89,976,532 (GRCm39)	N265K	probably damaging	Het
Mpp2	T	C	11: 101,952,904 (GRCm39)	D299G	probably benign	Het
Mtcl1	C	T	17: 66,745,197 (GRCm39)	G55R	probably damaging	Het
Myb	T	A	10: 21,028,431 (GRCm39)	K144I	probably damaging	Het
Myo18a	T	C	11: 77,709,875 (GRCm39)	I587T	possibly damaging	Het
Nr2c2	A	G	6: 92,135,374 (GRCm39)	S288G	probably benign	Het
Obscn	A	G	11: 58,969,128 (GRCm39)	Y2700H	probably damaging	Het
Or2z9	T	C	8: 72,854,345 (GRCm39)	V247A	probably benign	Het
Or6c3	T	A	10: 129,309,023 (GRCm39)	F154Y	probably damaging	Het
Pde6b	A	G	5: 108,536,574 (GRCm39)	D90G	probably damaging	Het
Pigo	A	G	4: 43,022,627 (GRCm39)	F315L	possibly damaging	Het
Prrc2c	TTGCTGCTGCTGCTGCTGCTGCTGCTGC	TTGCTGCTGCTGCTGCTGCTGCTGC	1: 162,536,630 (GRCm39)		probably benign	Het
Ret	T	C	6: 118,132,362 (GRCm39)	D1026G	probably damaging	Het
Rptn	A	T	3: 93,303,096 (GRCm39)	Q143L	probably benign	Het
Rubcnl	A	G	14: 75,272,910 (GRCm39)	E210G	probably benign	Het
Serpina1b	T	A	12: 103,698,712 (GRCm39)	T46S	probably benign	Het
Serpinb9e	A	T	13: 33,435,609 (GRCm39)	H14L	probably benign	Het
Shoc1	A	G	4: 59,059,652 (GRCm39)	S1003P	possibly damaging	Het
Slc25a25	A	C	2: 32,310,674 (GRCm39)	V168G	probably benign	Het
Slc35e1	T	C	8: 73,238,726 (GRCm39)	T261A	possibly damaging	Het
Spocd1	T	C	4: 129,849,197 (GRCm39)		probably benign	Het
Tmem222	A	T	4: 132,995,514 (GRCm39)	Y178N	probably damaging	Het
Top1mt	C	T	15: 75,535,893 (GRCm39)	E470K	probably benign	Het
Traj42	T	C	14: 54,413,290 (GRCm39)		probably benign	Het
Tshr	T	C	12: 91,504,876 (GRCm39)	Y13H	probably damaging	Het
Uvssa	A	G	5: 33,566,461 (GRCm39)		probably null	Het
Vmn1r28	G	A	6: 58,242,633 (GRCm39)	V159I	probably benign	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85,506,795 (GRCm39)	missense
IGL01151:Vmn2r73	APN	7	85,507,086 (GRCm39)	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85,521,455 (GRCm39)	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85,507,267 (GRCm39)	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85,506,757 (GRCm39)	makesense	probably null
IGL01818:Vmn2r73	APN	7	85,519,109 (GRCm39)	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85,521,902 (GRCm39)	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85,520,847 (GRCm39)	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85,525,007 (GRCm39)	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85,518,980 (GRCm39)	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85,507,123 (GRCm39)	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85,507,383 (GRCm39)	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85,522,084 (GRCm39)	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85,507,495 (GRCm39)	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85,519,468 (GRCm39)	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85,525,075 (GRCm39)	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R0413:Vmn2r73	UTSW	7	85,521,087 (GRCm39)	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R1523:Vmn2r73	UTSW	7	85,519,486 (GRCm39)	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85,525,120 (GRCm39)	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85,506,889 (GRCm39)	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85,507,375 (GRCm39)	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85,506,936 (GRCm39)	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85,506,975 (GRCm39)	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85,521,431 (GRCm39)	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85,520,871 (GRCm39)	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85,521,198 (GRCm39)	missense	probably benign
R3838:Vmn2r73	UTSW	7	85,507,258 (GRCm39)	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85,507,144 (GRCm39)	missense	probably benign
R4030:Vmn2r73	UTSW	7	85,521,044 (GRCm39)	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85,521,883 (GRCm39)	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85,520,768 (GRCm39)	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85,506,981 (GRCm39)	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85,519,449 (GRCm39)	missense	probably benign
R4552:Vmn2r73	UTSW	7	85,525,055 (GRCm39)	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85,520,923 (GRCm39)	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85,506,829 (GRCm39)	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85,507,155 (GRCm39)	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85,525,046 (GRCm39)	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85,518,996 (GRCm39)	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85,507,575 (GRCm39)	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85,507,299 (GRCm39)	missense	probably damaging	1.00
R5826:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R6018:Vmn2r73	UTSW	7	85,521,875 (GRCm39)	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85,507,429 (GRCm39)	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R6233:Vmn2r73	UTSW	7	85,519,099 (GRCm39)	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R6283:Vmn2r73	UTSW	7	85,521,049 (GRCm39)	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85,506,828 (GRCm39)	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85,519,544 (GRCm39)	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85,519,563 (GRCm39)	missense	probably benign	0.00
R6921:Vmn2r73	UTSW	7	85,507,446 (GRCm39)	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85,507,663 (GRCm39)	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85,522,075 (GRCm39)	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85,521,192 (GRCm39)	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85,521,147 (GRCm39)	missense	probably benign
R7811:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85,507,619 (GRCm39)	missense	probably benign
R8301:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85,507,128 (GRCm39)	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85,519,638 (GRCm39)	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85,521,902 (GRCm39)	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85,521,095 (GRCm39)	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85,507,668 (GRCm39)	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85,521,179 (GRCm39)	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85,522,149 (GRCm39)	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85,507,284 (GRCm39)	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85,521,904 (GRCm39)	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85,521,931 (GRCm39)	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85,507,123 (GRCm39)	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85,518,987 (GRCm39)	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85,519,073 (GRCm39)	missense	probably benign
R9448:Vmn2r73	UTSW	7	85,522,027 (GRCm39)	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85,506,795 (GRCm39)	missense
R9589:Vmn2r73	UTSW	7	85,519,659 (GRCm39)	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85,507,664 (GRCm39)	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85,521,176 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85,521,480 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAATGTCAAGAGCTTGGCTTC -3'
(R):5'- TCATCTGTGTCCTGCAGCAG -3'

Sequencing Primer
(F):5'- CAAGAGCTTGGCTTCATTGAATG -3'
(R):5'- TCCTGCAGCAGAGCACATTTG -3'

Posted On

2018-10-18