Incidental Mutation 'R6887:Adgrl4'
| ID |
536985 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Adgrl4
|
| Ensembl Gene |
ENSMUSG00000039167 |
| Gene Name |
adhesion G protein-coupled receptor L4 |
| Synonyms |
Eltd1, Etl, EGF-TM7 receptor, 1110033N21Rik |
| MMRRC Submission |
044981-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R6887 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
3 |
| Chromosomal Location |
151437887-151545086 bp(+) (GRCm38) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 151542733 bp (GRCm38)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Phenylalanine
at position 681
(I681F)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000041939
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000046977]
|
| AlphaFold |
Q923X1 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000046977
AA Change: I681F
PolyPhen 2
Score 0.458 (Sensitivity: 0.89; Specificity: 0.90)
|
| SMART Domains |
Protein: ENSMUSP00000041939
Gene: ENSMUSG00000039167
AA Change: I681F
| Domain | Start | End | E-Value | Type |
|---|
|
EGF
|
21 |
57 |
9.13e0 |
SMART |
|
EGF_CA
|
58 |
107 |
4.88e-9 |
SMART |
|
EGF_CA
|
108 |
157 |
4.88e-9 |
SMART |
|
Pfam:GAIN
|
182 |
390 |
6.8e-38 |
PFAM |
|
GPS
|
414 |
467 |
1.25e-17 |
SMART |
|
Pfam:7tm_2
|
473 |
709 |
2.5e-58 |
PFAM |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 99.9%
- 10x: 99.4%
- 20x: 98.1%
|
| Validation Efficiency |
100% (39/39) |
| MGI Phenotype |
PHENOTYPE: For a targeted mutation, no significant differences were detected between homozygous mice and controls in a high-throughput screen. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 39 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acan |
G |
A |
7: 79,092,483 (GRCm38) |
V543I |
probably damaging |
Het |
| Adcy5 |
A |
G |
16: 35,298,590 (GRCm38) |
I1104V |
possibly damaging |
Het |
| Adgrv1 |
T |
A |
13: 81,528,701 (GRCm38) |
M2004L |
probably benign |
Het |
| Anapc1 |
A |
G |
2: 128,659,768 (GRCm38) |
S785P |
possibly damaging |
Het |
| Ap3d1 |
A |
T |
10: 80,723,698 (GRCm38) |
I242N |
probably damaging |
Het |
| Arhgap5 |
T |
C |
12: 52,519,144 (GRCm38) |
L966P |
probably benign |
Het |
| Atp8a1 |
T |
C |
5: 67,738,451 (GRCm38) |
T547A |
probably benign |
Het |
| Cadps |
A |
G |
14: 12,505,811 (GRCm38) |
F753S |
probably damaging |
Het |
| Cdc20b |
T |
C |
13: 113,078,653 (GRCm38) |
S252P |
possibly damaging |
Het |
| Cep63 |
A |
G |
9: 102,625,927 (GRCm38) |
|
probably benign |
Het |
| Chrna5 |
G |
T |
9: 55,005,133 (GRCm38) |
V302L |
probably benign |
Het |
| Crtc2 |
A |
G |
3: 90,261,071 (GRCm38) |
T374A |
probably damaging |
Het |
| Dmtf1 |
T |
G |
5: 9,137,149 (GRCm38) |
D140A |
probably damaging |
Het |
| Exosc8 |
C |
T |
3: 54,733,699 (GRCm38) |
V39M |
probably damaging |
Het |
| Fam135b |
A |
C |
15: 71,463,315 (GRCm38) |
S677A |
probably damaging |
Het |
| Hif1an |
T |
C |
19: 44,563,389 (GRCm38) |
Y93H |
probably damaging |
Het |
| Hrc |
T |
C |
7: 45,335,664 (GRCm38) |
F80L |
probably benign |
Het |
| Jmjd1c |
A |
G |
10: 67,189,820 (GRCm38) |
T139A |
possibly damaging |
Het |
| Kdr |
T |
G |
5: 75,968,451 (GRCm38) |
R178S |
probably benign |
Het |
| Lrrc61 |
A |
C |
6: 48,568,432 (GRCm38) |
N63T |
probably damaging |
Het |
| Mrc1 |
C |
T |
2: 14,325,237 (GRCm38) |
A1219V |
possibly damaging |
Het |
| Neto1 |
T |
C |
18: 86,498,635 (GRCm38) |
V359A |
probably benign |
Het |
| Ngly1 |
T |
C |
14: 16,281,836 (GRCm38) |
I364T |
probably benign |
Het |
| Nisch |
C |
T |
14: 31,185,344 (GRCm38) |
|
probably benign |
Het |
| Olfr1373 |
C |
A |
11: 52,145,352 (GRCm38) |
M59I |
probably benign |
Het |
| Prrc2a |
T |
C |
17: 35,155,675 (GRCm38) |
D1333G |
probably damaging |
Het |
| Raly |
G |
T |
2: 154,861,910 (GRCm38) |
V134F |
probably damaging |
Het |
| Rbm6 |
T |
C |
9: 107,852,231 (GRCm38) |
Y406C |
probably damaging |
Het |
| Robo4 |
G |
C |
9: 37,402,067 (GRCm38) |
E6Q |
possibly damaging |
Het |
| Scnn1g |
C |
A |
7: 121,760,444 (GRCm38) |
S383R |
probably benign |
Het |
| Sgtb |
T |
C |
13: 104,111,151 (GRCm38) |
W13R |
probably benign |
Het |
| Slit3 |
T |
C |
11: 35,544,806 (GRCm38) |
|
probably null |
Het |
| Tbc1d32 |
G |
A |
10: 56,151,811 (GRCm38) |
Q732* |
probably null |
Het |
| Tek |
T |
A |
4: 94,804,944 (GRCm38) |
C247S |
probably damaging |
Het |
| Tmf1 |
A |
T |
6: 97,176,838 (GRCm38) |
D91E |
probably damaging |
Het |
| Usp39 |
A |
G |
6: 72,333,157 (GRCm38) |
L326P |
probably damaging |
Het |
| Vmn2r7 |
A |
T |
3: 64,690,827 (GRCm38) |
C770S |
probably damaging |
Het |
| Wdr31 |
C |
T |
4: 62,457,565 (GRCm38) |
G58R |
probably benign |
Het |
| Zfyve26 |
A |
G |
12: 79,266,449 (GRCm38) |
I54T |
probably damaging |
Het |
|
| Other mutations in Adgrl4 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00488:Adgrl4
|
APN |
3 |
151,542,841 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL00694:Adgrl4
|
APN |
3 |
151,439,396 (GRCm38) |
splice site |
probably benign |
|
|
IGL01143:Adgrl4
|
APN |
3 |
151,500,229 (GRCm38) |
splice site |
probably null |
|
|
IGL01359:Adgrl4
|
APN |
3 |
151,543,286 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL01947:Adgrl4
|
APN |
3 |
151,510,791 (GRCm38) |
critical splice donor site |
probably null |
|
|
IGL02149:Adgrl4
|
APN |
3 |
151,500,354 (GRCm38) |
missense |
possibly damaging |
0.95 |
|
IGL02324:Adgrl4
|
APN |
3 |
151,497,874 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02562:Adgrl4
|
APN |
3 |
151,439,312 (GRCm38) |
missense |
probably damaging |
1.00 |
|
IGL02644:Adgrl4
|
APN |
3 |
151,492,370 (GRCm38) |
missense |
probably benign |
0.00 |
|
trivial
|
UTSW |
3 |
151,517,610 (GRCm38) |
missense |
probably benign |
0.07 |
|
R0077:Adgrl4
|
UTSW |
3 |
151,517,781 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R0116:Adgrl4
|
UTSW |
3 |
151,517,610 (GRCm38) |
missense |
probably benign |
0.07 |
|
R0331:Adgrl4
|
UTSW |
3 |
151,497,940 (GRCm38) |
missense |
probably benign |
0.00 |
|
R0601:Adgrl4
|
UTSW |
3 |
151,498,429 (GRCm38) |
splice site |
probably benign |
|
|
R0613:Adgrl4
|
UTSW |
3 |
151,543,222 (GRCm38) |
splice site |
probably benign |
|
|
R1293:Adgrl4
|
UTSW |
3 |
151,507,444 (GRCm38) |
missense |
probably benign |
0.00 |
|
R1463:Adgrl4
|
UTSW |
3 |
151,510,596 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R1697:Adgrl4
|
UTSW |
3 |
151,517,611 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1731:Adgrl4
|
UTSW |
3 |
151,540,986 (GRCm38) |
missense |
possibly damaging |
0.64 |
|
R1765:Adgrl4
|
UTSW |
3 |
151,543,235 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R1782:Adgrl4
|
UTSW |
3 |
151,542,805 (GRCm38) |
nonsense |
probably null |
|
|
R1888:Adgrl4
|
UTSW |
3 |
151,439,277 (GRCm38) |
missense |
probably benign |
0.11 |
|
R1888:Adgrl4
|
UTSW |
3 |
151,439,277 (GRCm38) |
missense |
probably benign |
0.11 |
|
R1957:Adgrl4
|
UTSW |
3 |
151,510,779 (GRCm38) |
missense |
possibly damaging |
0.94 |
|
R2128:Adgrl4
|
UTSW |
3 |
151,500,201 (GRCm38) |
missense |
probably benign |
0.00 |
|
R2180:Adgrl4
|
UTSW |
3 |
151,500,142 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R2238:Adgrl4
|
UTSW |
3 |
151,500,142 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R2474:Adgrl4
|
UTSW |
3 |
151,542,724 (GRCm38) |
missense |
probably benign |
0.01 |
|
R2697:Adgrl4
|
UTSW |
3 |
151,510,623 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R3835:Adgrl4
|
UTSW |
3 |
151,510,617 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R4499:Adgrl4
|
UTSW |
3 |
151,510,785 (GRCm38) |
missense |
possibly damaging |
0.81 |
|
R4640:Adgrl4
|
UTSW |
3 |
151,500,310 (GRCm38) |
unclassified |
probably benign |
|
|
R4747:Adgrl4
|
UTSW |
3 |
151,507,440 (GRCm38) |
missense |
probably benign |
0.01 |
|
R5428:Adgrl4
|
UTSW |
3 |
151,542,686 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R5510:Adgrl4
|
UTSW |
3 |
151,497,830 (GRCm38) |
missense |
possibly damaging |
0.89 |
|
R5717:Adgrl4
|
UTSW |
3 |
151,492,334 (GRCm38) |
missense |
probably benign |
0.01 |
|
R6106:Adgrl4
|
UTSW |
3 |
151,540,985 (GRCm38) |
missense |
possibly damaging |
0.67 |
|
R6343:Adgrl4
|
UTSW |
3 |
151,517,806 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6419:Adgrl4
|
UTSW |
3 |
151,439,316 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R6468:Adgrl4
|
UTSW |
3 |
151,492,375 (GRCm38) |
missense |
probably benign |
|
|
R6636:Adgrl4
|
UTSW |
3 |
151,517,773 (GRCm38) |
nonsense |
probably null |
|
|
R6637:Adgrl4
|
UTSW |
3 |
151,517,773 (GRCm38) |
nonsense |
probably null |
|
|
R6687:Adgrl4
|
UTSW |
3 |
151,542,755 (GRCm38) |
missense |
probably benign |
0.00 |
|
R6856:Adgrl4
|
UTSW |
3 |
151,500,118 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7041:Adgrl4
|
UTSW |
3 |
151,439,322 (GRCm38) |
missense |
probably benign |
0.00 |
|
R7527:Adgrl4
|
UTSW |
3 |
151,439,250 (GRCm38) |
missense |
probably benign |
0.08 |
|
R7597:Adgrl4
|
UTSW |
3 |
151,543,258 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R7751:Adgrl4
|
UTSW |
3 |
151,492,309 (GRCm38) |
missense |
probably damaging |
0.99 |
|
R8556:Adgrl4
|
UTSW |
3 |
151,510,665 (GRCm38) |
missense |
probably damaging |
0.98 |
|
R8696:Adgrl4
|
UTSW |
3 |
151,542,707 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8698:Adgrl4
|
UTSW |
3 |
151,497,875 (GRCm38) |
missense |
probably damaging |
1.00 |
|
R8795:Adgrl4
|
UTSW |
3 |
151,510,779 (GRCm38) |
missense |
probably benign |
0.03 |
|
R9226:Adgrl4
|
UTSW |
3 |
151,492,427 (GRCm38) |
critical splice donor site |
probably null |
|
|
R9655:Adgrl4
|
UTSW |
3 |
151,542,813 (GRCm38) |
missense |
probably damaging |
0.96 |
|
R9755:Adgrl4
|
UTSW |
3 |
151,510,781 (GRCm38) |
missense |
probably benign |
0.01 |
|
R9767:Adgrl4
|
UTSW |
3 |
151,501,757 (GRCm38) |
missense |
probably benign |
0.16 |
|
R9784:Adgrl4
|
UTSW |
3 |
151,509,311 (GRCm38) |
missense |
probably damaging |
1.00 |
|
X0053:Adgrl4
|
UTSW |
3 |
151,497,833 (GRCm38) |
missense |
probably damaging |
1.00 |
|
Z1088:Adgrl4
|
UTSW |
3 |
151,500,175 (GRCm38) |
missense |
probably benign |
0.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGTTGCATCAAACCACAAGC -3'
(R):5'- GTCACACTTCAAGGCAACAG -3'
Sequencing Primer
(F):5'- CCACAAGCCAGCTATGAGC -3'
(R):5'- GTCACACTTCAAGGCAACAGTTTTTC -3'
|
| Posted On |
2018-10-18 |
Incidental Mutation