Mutagenetix > Incidental Mutation

Incidental Mutation 'R6888:Zfp882'

537052

Institutional Source

Beutler Lab

Gene Symbol

Zfp882

Ensembl Gene

ENSMUSG00000089857

Gene Name

zinc finger protein 882

Synonyms

ENSMUSG00000052439

MMRRC Submission

044982-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.092) question?

Stock #

R6888 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

71908608-71916354 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 71914286 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tyrosine at position 319 (C319Y)

Ref Sequence

ENSEMBL: ENSMUSP00000105629 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000110002] [ENSMUST00000125802] [ENSMUST00000126607] [ENSMUST00000131544]

AlphaFold

E9Q4R4

Predicted Effect

probably benign
Transcript: ENSMUST00000110002
AA Change: C319Y

PolyPhen 2 Score 0.011 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000105629
Gene: ENSMUSG00000089857
AA Change: C319Y

Domain	Start	End	E-Value	Type
KRAB	4	61	8.58e-14	SMART
ZnF_C2H2	84	106	1.47e-3	SMART
ZnF_C2H2	168	190	8.47e-4	SMART
ZnF_C2H2	196	218	2.27e-4	SMART
ZnF_C2H2	224	246	6.31e1	SMART
ZnF_C2H2	251	273	1.16e-1	SMART
ZnF_C2H2	279	301	1.25e-1	SMART
ZnF_C2H2	307	329	5.42e-2	SMART
ZnF_C2H2	335	357	1.47e-3	SMART
ZnF_C2H2	363	385	7.26e-3	SMART
ZnF_C2H2	391	413	1.26e-2	SMART
ZnF_C2H2	419	441	3.29e1	SMART
ZnF_C2H2	447	469	2.67e-1	SMART
ZnF_C2H2	475	497	1.04e-3	SMART
ZnF_C2H2	503	525	4.11e-2	SMART
ZnF_C2H2	531	553	4.87e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125802

SMART Domains

Protein: ENSMUSP00000121316
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	12	69	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000126607

SMART Domains

Protein: ENSMUSP00000119978
Gene: ENSMUSG00000089857

Domain	Start	End	E-Value	Type
KRAB	44	101	8.58e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131544

SMART Domains

Protein: ENSMUSP00000120213
Gene: ENSMUSG00000066880

Domain	Start	End	E-Value	Type
KRAB	4	56	1.08e-10	SMART
ZnF_C2H2	167	189	8.47e-4	SMART
ZnF_C2H2	195	217	8.34e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110051M20Rik	A	G	2: 91,421,894 (GRCm38)	Y41H	probably damaging	Het
Actg1	T	C	11: 120,347,315 (GRCm38)	Y190C	probably damaging	Het
Adgrv1	A	G	13: 81,508,669 (GRCm38)	I2902T	probably damaging	Het
AI429214	G	A	8: 36,993,833 (GRCm38)	G45D	possibly damaging	Het
Ambra1	A	G	2: 91,769,027 (GRCm38)	D164G	probably damaging	Het
Ap3b1	A	T	13: 94,408,791 (GRCm38)	Q184L	probably benign	Het
Arhgef17	T	C	7: 100,930,820 (GRCm38)	D307G	possibly damaging	Het
AY761185	A	T	8: 20,944,555 (GRCm38)	Y52*	probably null	Het
Bfsp1	A	G	2: 143,826,719 (GRCm38)	S647P	probably benign	Het
C130073F10Rik	A	G	4: 101,890,256 (GRCm38)	V192A	probably benign	Het
Cacna1g	T	A	11: 94,459,207 (GRCm38)	D604V	probably benign	Het
Ccdc170	T	C	10: 4,546,854 (GRCm38)	V458A	possibly damaging	Het
Ccdc187	G	A	2: 26,289,734 (GRCm38)	R238W	probably damaging	Het
Cdh1	A	G	8: 106,658,314 (GRCm38)	S380G	probably benign	Het
Cdk5rap3	T	C	11: 96,916,192 (GRCm38)	H4R	probably benign	Het
Cftr	G	A	6: 18,313,730 (GRCm38)		probably null	Het
Dnajc16	A	G	4: 141,776,992 (GRCm38)	V219A	probably benign	Het
Drd3	A	T	16: 43,817,139 (GRCm38)	I266F	probably benign	Het
Dut	A	G	2: 125,257,124 (GRCm38)	D177G	probably benign	Het
Esr1	A	G	10: 4,857,076 (GRCm38)	I331V	probably benign	Het
Fam129c	G	T	8: 71,603,739 (GRCm38)	R361L	probably benign	Het
Gm11110	A	G	17: 57,102,143 (GRCm38)		probably benign	Het
Grm7	G	T	6: 111,358,353 (GRCm38)	G575V	possibly damaging	Het
Heatr3	A	G	8: 88,170,884 (GRCm38)	Y531C	probably damaging	Het
Igfn1	A	G	1: 135,982,480 (GRCm38)	V122A	probably benign	Het
Igsf21	T	C	4: 140,034,743 (GRCm38)	D208G	probably benign	Het
Kntc1	T	C	5: 123,811,310 (GRCm38)	Y1915H	probably damaging	Het
Lamc1	A	T	1: 153,262,492 (GRCm38)	D205E	probably damaging	Het
Limch1	C	T	5: 67,021,926 (GRCm38)	T713I	probably benign	Het
Lrp1b	A	G	2: 41,471,126 (GRCm38)	I555T	probably benign	Het
Lrp2	A	T	2: 69,524,141 (GRCm38)	F448I	probably damaging	Het
March6	T	C	15: 31,459,233 (GRCm38)	K896E	probably benign	Het
Mpp6	T	G	6: 50,180,277 (GRCm38)		probably null	Het
Mroh4	T	C	15: 74,613,249 (GRCm38)	Y469C	possibly damaging	Het
Nos3	T	C	5: 24,383,335 (GRCm38)	V1060A	possibly damaging	Het
Nr1h4	A	G	10: 89,456,542 (GRCm38)	I406T	probably damaging	Het
Odf2l	T	C	3: 145,148,618 (GRCm38)		probably null	Het
Olfr1162	A	G	2: 88,050,264 (GRCm38)	M120T	probably damaging	Het
Olfr1387	A	T	11: 49,460,260 (GRCm38)	T194S	probably damaging	Het
Pbx2	A	G	17: 34,594,107 (GRCm38)	Y119C	possibly damaging	Het
Pdcd6ip	C	A	9: 113,671,837 (GRCm38)	A526S	probably benign	Het
Prx	G	A	7: 27,519,634 (GRCm38)	D1187N	possibly damaging	Het
Ptpro	G	A	6: 137,380,200 (GRCm38)	V230I	probably benign	Het
Rtn3	C	T	19: 7,457,249 (GRCm38)	M440I	probably benign	Het
Sar1b	T	C	11: 51,788,192 (GRCm38)	I96T	probably damaging	Het
Sds	T	C	5: 120,480,900 (GRCm38)		probably null	Het
Secisbp2	A	G	13: 51,679,941 (GRCm38)	T706A	probably benign	Het
Sorcs3	A	T	19: 48,693,824 (GRCm38)	M433L	possibly damaging	Het
Sppl2a	A	G	2: 126,904,992 (GRCm38)	V472A	probably damaging	Het
Tbc1d9	A	G	8: 83,271,588 (GRCm38)	E1258G	possibly damaging	Het
Tbxas1	C	A	6: 38,952,074 (GRCm38)		probably benign	Het
Tg	T	C	15: 66,696,246 (GRCm38)	I1333T	probably damaging	Het
Tmem108	C	T	9: 103,499,716 (GRCm38)	G178D	probably damaging	Het
Tmem174	A	G	13: 98,637,061 (GRCm38)	L87P	probably damaging	Het
Tmppe	T	A	9: 114,404,701 (GRCm38)	S23T	probably damaging	Het
Ttn	G	A	2: 76,761,103 (GRCm38)	T21074I	probably benign	Het
Tub	A	G	7: 109,029,298 (GRCm38)	M338V	probably null	Het
Vmn1r160	A	T	7: 22,872,106 (GRCm38)	R295*	probably null	Het
Vps52	T	C	17: 33,963,206 (GRCm38)	V518A	probably benign	Het
Wdr59	A	G	8: 111,451,043 (GRCm38)	V909A	probably benign	Het
Wnk1	T	C	6: 119,948,781 (GRCm38)	T1241A	probably benign	Het
Znfx1	A	T	2: 167,038,940 (GRCm38)	I308N	possibly damaging	Het

Other mutations in Zfp882

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00676:Zfp882	APN	8	71,913,827 (GRCm38)	missense	probably benign
R0244:Zfp882	UTSW	8	71,913,523 (GRCm38)	missense	possibly damaging	0.79
R0270:Zfp882	UTSW	8	71,914,615 (GRCm38)	missense	probably benign	0.05
R0636:Zfp882	UTSW	8	71,914,337 (GRCm38)	missense	probably benign	0.01
R0840:Zfp882	UTSW	8	71,914,686 (GRCm38)	nonsense	probably null
R1299:Zfp882	UTSW	8	71,913,473 (GRCm38)	missense	probably damaging	1.00
R4439:Zfp882	UTSW	8	71,913,609 (GRCm38)	missense	probably damaging	0.97
R4829:Zfp882	UTSW	8	71,914,389 (GRCm38)	missense	probably damaging	1.00
R5028:Zfp882	UTSW	8	71,914,654 (GRCm38)	missense	possibly damaging	0.70
R5296:Zfp882	UTSW	8	71,914,360 (GRCm38)	missense	probably damaging	1.00
R5882:Zfp882	UTSW	8	71,913,459 (GRCm38)	critical splice acceptor site	probably null
R5974:Zfp882	UTSW	8	71,913,155 (GRCm38)	missense	probably damaging	1.00
R6052:Zfp882	UTSW	8	71,914,505 (GRCm38)	missense	probably benign	0.01
R6383:Zfp882	UTSW	8	71,914,640 (GRCm38)	missense	probably damaging	1.00
R6987:Zfp882	UTSW	8	71,914,673 (GRCm38)	missense	probably benign	0.01
R7045:Zfp882	UTSW	8	71,913,249 (GRCm38)	critical splice donor site	probably null
R7780:Zfp882	UTSW	8	71,914,229 (GRCm38)	missense	possibly damaging	0.89
R7793:Zfp882	UTSW	8	71,913,141 (GRCm38)	missense	probably damaging	1.00
R8386:Zfp882	UTSW	8	71,914,118 (GRCm38)	missense	probably benign	0.00
R9452:Zfp882	UTSW	8	71,914,987 (GRCm38)	missense	probably damaging	1.00
R9694:Zfp882	UTSW	8	71,914,071 (GRCm38)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTTTGAGGAAGGTTGAAAGTCATG -3'
(R):5'- CCTGTGAATCTGTTCATGGTATC -3'

Sequencing Primer
(F):5'- GGGATAGCCTTCAGTGATCAC -3'
(R):5'- GAATCTGTTCATGGTATCGAAGAGC -3'

Posted On

2018-10-18