Mutagenetix > Incidental Mutation

Incidental Mutation 'R6889:Olfr574'

537108

Institutional Source

Beutler Lab

Gene Symbol

Olfr574

Ensembl Gene

ENSMUSG00000045824

Gene Name

olfactory receptor 574

Synonyms

GA_x6K02T2PBJ9-5659738-5660748, MOR14-9

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.144) question?

Stock #

R6889 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

102948467-102949507 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 102948768 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 91 (H91L)

Ref Sequence

ENSEMBL: ENSMUSP00000147694 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052997] [ENSMUST00000211329] [ENSMUST00000213477] [ENSMUST00000216420]

AlphaFold

F8VQ18

Predicted Effect

possibly damaging
Transcript: ENSMUST00000052997
AA Change: H101L

PolyPhen 2 Score 0.941 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000095815
Gene: ENSMUSG00000045824
AA Change: H101L

Domain	Start	End	E-Value	Type
low complexity region	14	31	N/A	INTRINSIC
Pfam:7tm_4	44	323	3.8e-96	PFAM
Pfam:7TM_GPCR_Srsx	48	195	1.2e-8	PFAM
Pfam:7tm_1	54	305	2e-17	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000211329
AA Change: H91L

PolyPhen 2 Score 0.941 (Sensitivity: 0.80; Specificity: 0.94)

Predicted Effect

probably benign
Transcript: ENSMUST00000213477

Predicted Effect

probably benign
Transcript: ENSMUST00000216420

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310002L09Rik	A	T	4: 73,943,053	D103E	probably benign	Het
Abcc3	A	T	11: 94,375,555	S70T	possibly damaging	Het
Atp6v0a1	A	G	11: 101,029,183	Y214C	possibly damaging	Het
Azi2	A	G	9: 118,049,895		probably null	Het
BC067074	C	A	13: 113,318,378	S319R	probably damaging	Het
Cacnb2	T	A	2: 14,986,015	V636E	possibly damaging	Het
Cd8a	A	C	6: 71,374,562	T169P	probably damaging	Het
Cfap44	G	A	16: 44,404,132	V68I	probably benign	Het
Eea1	G	T	10: 96,037,478	C1134F	probably benign	Het
Ehmt2	T	G	17: 34,912,772	F1192V	probably damaging	Het
Emc1	T	C	4: 139,365,350	F531L	probably damaging	Het
Eme1	G	A	11: 94,650,477	T173I	probably benign	Het
Gli2	A	T	1: 118,844,416	C520S	probably damaging	Het
Gm43302	T	C	5: 105,280,138	K186E	probably benign	Het
Gpr108	A	T	17: 57,236,990	N405K	probably damaging	Het
Hmgcl	C	A	4: 135,955,642	T135N	probably benign	Het
Hydin	G	A	8: 110,532,856	D2487N	possibly damaging	Het
Igfl3	G	T	7: 18,179,800	R25L	probably benign	Het
Igsf10	A	C	3: 59,331,933	S276A	probably benign	Het
Kctd1	A	G	18: 14,973,988	S211P	probably damaging	Het
Kctd7	A	T	5: 130,152,501	Q255L	probably benign	Het
Lrig1	A	G	6: 94,625,063	Y270H	probably benign	Het
Muc5ac	C	T	7: 141,809,744		probably benign	Het
Myh15	A	G	16: 49,153,111	N1248S	possibly damaging	Het
Nod1	A	G	6: 54,944,109	F408S	probably benign	Het
Nrp1	T	C	8: 128,493,057	F652S	probably damaging	Het
Olfr1338	G	A	4: 118,754,307	T79I	probably damaging	Het
Olfr487	A	T	7: 108,211,918	F204I	probably benign	Het
Olfr821	T	C	10: 130,034,532	M302T	probably benign	Het
Opa1	G	T	16: 29,620,868	R792L	probably benign	Het
Pcdha6	G	T	18: 36,968,343	L196F	probably damaging	Het
Pdia2	A	T	17: 26,196,970	Y347*	probably null	Het
Pdpr	G	T	8: 111,124,613		probably null	Het
Pigt	T	A	2: 164,507,331	L518Q	probably damaging	Het
Ppfibp2	A	C	7: 107,737,981	D591A	possibly damaging	Het
Prrg2	G	A	7: 45,059,989	T97M	possibly damaging	Het
Qars	A	G	9: 108,513,183	T428A	probably damaging	Het
Rai1	T	C	11: 60,185,715	F202L	probably damaging	Het
Rars	A	T	11: 35,808,486	M660K	probably damaging	Het
Rsf1	GCGGCGGCG	GCGGCGGCGTCGGCGGCG	7: 97,579,925		probably benign	Het
Slc16a6	A	C	11: 109,455,040	F382V	probably damaging	Het
Slc30a7	T	C	3: 115,954,153	T330A	probably damaging	Het
Smc1b	A	T	15: 85,067,759	L1157Q	probably damaging	Het
Snx4	G	T	16: 33,251,470	A4S	possibly damaging	Het
Sv2b	A	C	7: 75,125,767		probably null	Het
Syt9	A	T	7: 107,425,286	I129L	probably damaging	Het
Ttbk2	T	C	2: 120,773,353	E198G	probably damaging	Het
Ubr3	A	T	2: 69,944,300	D488V	possibly damaging	Het
Ush2a	T	A	1: 188,797,871	C3286S	probably damaging	Het
Vill	A	G	9: 119,065,882	D56G	possibly damaging	Het
Vmn1r41	A	T	6: 89,747,370	I298F	probably damaging	Het
Vmn2r2	A	T	3: 64,117,267	V631D	probably damaging	Het
Vmn2r32	A	G	7: 7,472,574	S437P	possibly damaging	Het
Vmn2r53	A	G	7: 12,601,142	V197A	probably benign	Het
Wasf1	A	T	10: 40,920,369	I32F	probably damaging	Het
Wasf2	G	T	4: 133,194,730	A387S	unknown	Het
Wdr92	G	A	11: 17,222,309	V133M	probably damaging	Het
Zbtb14	G	A	17: 69,387,679	C124Y	probably damaging	Het
Zfp462	G	T	4: 55,007,671	A37S	probably damaging	Het
Zfp532	A	G	18: 65,686,990	E882G	possibly damaging	Het

Other mutations in Olfr574

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01154:Olfr574	APN	7	102948839	missense	probably damaging	1.00
IGL01732:Olfr574	APN	7	102949239	missense	probably damaging	1.00
IGL02185:Olfr574	APN	7	102948514	missense	probably damaging	0.96
PIT4382001:Olfr574	UTSW	7	102949449	missense	probably benign
PIT4520001:Olfr574	UTSW	7	102948714	missense	probably damaging	1.00
R0765:Olfr574	UTSW	7	102948732	missense	probably damaging	1.00
R1616:Olfr574	UTSW	7	102948514	missense	probably damaging	0.96
R2041:Olfr574	UTSW	7	102948963	missense	probably damaging	0.98
R2079:Olfr574	UTSW	7	102949495	missense	probably benign	0.00
R2261:Olfr574	UTSW	7	102949257	missense	probably damaging	1.00
R2263:Olfr574	UTSW	7	102949257	missense	probably damaging	1.00
R2513:Olfr574	UTSW	7	102949493	missense	probably benign
R2903:Olfr574	UTSW	7	102949454	missense	probably benign	0.05
R4445:Olfr574	UTSW	7	102948798	missense	possibly damaging	0.93
R4512:Olfr574	UTSW	7	102948738	missense	probably damaging	1.00
R4513:Olfr574	UTSW	7	102948738	missense	probably damaging	1.00
R4528:Olfr574	UTSW	7	102948806	missense	probably damaging	1.00
R4904:Olfr574	UTSW	7	102949065	missense	probably damaging	1.00
R5935:Olfr574	UTSW	7	102948810	missense	probably benign	0.01
R6784:Olfr574	UTSW	7	102948515	missense	possibly damaging	0.91
R7082:Olfr574	UTSW	7	102949248	missense	probably damaging	1.00
R7125:Olfr574	UTSW	7	102949179	missense	probably damaging	0.99
R7549:Olfr574	UTSW	7	102948591	missense	possibly damaging	0.92
R7564:Olfr574	UTSW	7	102949266	missense	probably damaging	1.00
R7947:Olfr574	UTSW	7	102949071	missense	probably damaging	1.00
R8460:Olfr574	UTSW	7	102949324	nonsense	probably null
R8518:Olfr574	UTSW	7	102948597	missense	probably damaging	1.00
R8859:Olfr574	UTSW	7	102949166	missense	probably damaging	1.00
R9116:Olfr574	UTSW	7	102949320	missense	possibly damaging	0.87
R9327:Olfr574	UTSW	7	102948480	missense	probably benign	0.00
R9408:Olfr574	UTSW	7	102949236	missense	probably damaging	1.00
Z1177:Olfr574	UTSW	7	102948752	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGTCTCTACACCATTGCAC -3'
(R):5'- TGGAAGGACACACTCTTCAC -3'

Sequencing Primer
(F):5'- CCATTGCACTCTTAGGAAACAG -3'
(R):5'- TGTCTTATACAGATGATCAGTCCG -3'

Posted On

2018-10-18