|Institutional Source||Beutler Lab|
|Gene Name||dual specificity phosphatase 10|
|Synonyms||MKP5, 2610306G15Rik, MKP-5|
|Is this an essential gene?||Possibly essential (E-score: 0.640)|
|Stock #||R6890 (G1)|
|Chromosomal Location||184013302-184075636 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to G at 184069196 bp|
|Amino Acid Change||Tyrosine to Aspartic acid at position 387 (Y387D)|
|Ref Sequence||ENSEMBL: ENSMUSP00000045838 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000048655]|
|Predicted Effect||probably damaging
AA Change: Y387D
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: Y387D
|Meta Mutation Damage Score||0.8616|
|Coding Region Coverage||
|Validation Efficiency||100% (39/39)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for a knock-out allele display alterations in both innate and adaptive immune responses. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dusp10||
(F):5'- CTTCCTGTTCCTCGGCAATGAG -3'
(R):5'- ATTATGCCCATCCAGGTAGTTCTG -3'
(F):5'- TTCCTCGGCAATGAGCAGGATG -3'
(R):5'- CATCCAGGTAGTTCTGAGACTAACAG -3'