Mutagenetix > Incidental Mutation

Incidental Mutation 'R6820:Zfp735'

537575

Institutional Source

Beutler Lab

Gene Symbol

Zfp735

Ensembl Gene

ENSMUSG00000060630

Gene Name

zinc finger protein 735

Synonyms

1700012C15Rik

MMRRC Submission

044932-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R6820 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

73579604-73604624 bp(+) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to G at 73579783 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 1 (M1V)

Ref Sequence

ENSEMBL: ENSMUSP00000079269 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080407]

AlphaFold

B1ARH2

Predicted Effect

probably null
Transcript: ENSMUST00000080407
AA Change: M1V

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000079269
Gene: ENSMUSG00000060630
AA Change: M1V

Domain	Start	End	E-Value	Type
KRAB	8	68	2.2e-34	SMART
ZnF_C2H2	483	505	4.38e1	SMART
ZnF_C2H2	511	533	2.67e-1	SMART
ZnF_C2H2	539	561	1.81e1	SMART
ZnF_C2H2	567	589	1.5e-4	SMART
ZnF_C2H2	595	617	4.87e-4	SMART
ZnF_C2H2	623	645	4.24e-4	SMART
ZnF_C2H2	651	673	2.27e-4	SMART
ZnF_C2H2	679	701	7.49e-5	SMART
ZnF_C2H2	707	729	4.87e-4	SMART

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

98% (46/47)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Accs	T	A	2: 93,673,266 (GRCm39)	N141Y	probably null	Het
Chkb	A	G	15: 89,312,379 (GRCm39)	L46P	probably damaging	Het
Col9a3	G	A	2: 180,248,927 (GRCm39)	V260M	probably damaging	Het
Dna2	T	C	10: 62,800,683 (GRCm39)	I739T	possibly damaging	Het
Dnah17	T	C	11: 117,959,826 (GRCm39)	H2620R	probably damaging	Het
Dsel	A	G	1: 111,787,547 (GRCm39)	V996A	probably damaging	Het
Dst	T	C	1: 34,250,337 (GRCm39)	L1757S	probably damaging	Het
Exoc5	A	T	14: 49,286,387 (GRCm39)		probably null	Het
Fam120a	A	T	13: 49,034,468 (GRCm39)	V1048E	possibly damaging	Het
Fam50b	G	A	13: 34,931,084 (GRCm39)	E187K	possibly damaging	Het
Fbxw19	T	A	9: 109,311,079 (GRCm39)	T377S	probably benign	Het
Fbxw28	A	T	9: 109,167,493 (GRCm39)	F88Y	probably damaging	Het
Grik5	C	T	7: 24,745,780 (GRCm39)	R431Q	possibly damaging	Het
Gtsf1	T	C	15: 103,328,954 (GRCm39)	T92A	probably benign	Het
Hoxc13	A	G	15: 102,830,257 (GRCm39)	Y212C	probably damaging	Het
Itih2	T	C	2: 10,102,909 (GRCm39)	I742V	probably benign	Het
Kat7	T	C	11: 95,174,965 (GRCm39)	T351A	probably damaging	Het
Mlh3	T	C	12: 85,294,497 (GRCm39)	D1233G	probably damaging	Het
Mroh2b	A	G	15: 4,982,756 (GRCm39)	D1525G	probably damaging	Het
Nme5	T	C	18: 34,704,626 (GRCm39)	Y73C	probably damaging	Het
Nr3c2	T	C	8: 77,969,086 (GRCm39)	V957A	probably damaging	Het
Nup153	A	G	13: 46,863,459 (GRCm39)	S301P	probably benign	Het
Obscn	T	C	11: 58,942,019 (GRCm39)	D5013G	probably damaging	Het
Or4f54	C	T	2: 111,123,455 (GRCm39)	P281S	probably damaging	Het
Or5p51	A	G	7: 107,444,298 (GRCm39)	V214A	probably benign	Het
Or8g51	A	G	9: 38,608,771 (GRCm39)	V297A	possibly damaging	Het
Pak1	A	G	7: 97,535,586 (GRCm39)	N226D	probably benign	Het
Pak4	A	G	7: 28,262,461 (GRCm39)	Y384H	probably benign	Het
Pkp3	T	C	7: 140,659,757 (GRCm39)		probably null	Het
Prxl2b	A	T	4: 154,982,623 (GRCm39)	D50E	probably damaging	Het
Psd	G	T	19: 46,309,283 (GRCm39)	A558E	probably damaging	Het
Psmd14	C	A	2: 61,607,068 (GRCm39)	H172N	probably benign	Het
Pygb	G	T	2: 150,658,674 (GRCm39)	W366L	possibly damaging	Het
Rbm39	A	T	2: 156,021,146 (GRCm39)	M1K	probably null	Het
Rnf213	T	C	11: 119,339,664 (GRCm39)	I3421T	probably damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Smg6	T	C	11: 74,932,790 (GRCm39)	V88A	probably damaging	Het
Tha1	T	C	11: 117,762,504 (GRCm39)	E80G	probably benign	Het
Tie1	A	G	4: 118,341,583 (GRCm39)	V243A	probably damaging	Het
Tmem215	T	C	4: 40,473,926 (GRCm39)	M1T	probably null	Het
Tpm2	A	G	4: 43,518,443 (GRCm39)	Y221H	probably damaging	Het
Ubap1	C	T	4: 41,379,854 (GRCm39)	P356L	probably benign	Het
Wbp2nl	G	T	15: 82,197,996 (GRCm39)	A178S	possibly damaging	Het
Wdr54	A	T	6: 83,131,601 (GRCm39)	S139T	probably benign	Het
Wipi2	G	C	5: 142,615,555 (GRCm39)	Q14H	probably benign	Het
Zan	T	C	5: 137,406,106 (GRCm39)		probably benign	Het

Other mutations in Zfp735

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00492:Zfp735	APN	11	73,602,192 (GRCm39)	missense	possibly damaging	0.86
IGL00798:Zfp735	APN	11	73,602,386 (GRCm39)	missense	possibly damaging	0.72
IGL01642:Zfp735	APN	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
IGL01684:Zfp735	APN	11	73,581,191 (GRCm39)	missense	possibly damaging	0.86
IGL02096:Zfp735	APN	11	73,602,254 (GRCm39)	missense	probably benign	0.01
IGL02238:Zfp735	APN	11	73,601,319 (GRCm39)	missense	probably benign	0.00
IGL02505:Zfp735	APN	11	73,580,626 (GRCm39)	missense	probably benign	0.03
IGL02740:Zfp735	APN	11	73,601,412 (GRCm39)	missense	possibly damaging	0.53
IGL02957:Zfp735	APN	11	73,601,755 (GRCm39)	missense	probably benign	0.00
bananaquit	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
bescher	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
Galvanic	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
grassquit	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R0114:Zfp735	UTSW	11	73,601,488 (GRCm39)	missense	probably benign	0.33
R0217:Zfp735	UTSW	11	73,602,112 (GRCm39)	missense	possibly damaging	0.73
R0943:Zfp735	UTSW	11	73,602,909 (GRCm39)	missense	probably benign	0.04
R1421:Zfp735	UTSW	11	73,601,523 (GRCm39)	missense	probably benign
R1460:Zfp735	UTSW	11	73,603,159 (GRCm39)	missense	possibly damaging	0.73
R1493:Zfp735	UTSW	11	73,601,305 (GRCm39)	missense	possibly damaging	0.73
R1517:Zfp735	UTSW	11	73,601,470 (GRCm39)	missense	probably benign
R1676:Zfp735	UTSW	11	73,602,301 (GRCm39)	missense	possibly damaging	0.53
R1709:Zfp735	UTSW	11	73,602,589 (GRCm39)	missense	probably benign	0.01
R1871:Zfp735	UTSW	11	73,601,412 (GRCm39)	nonsense	probably null
R1931:Zfp735	UTSW	11	73,602,677 (GRCm39)	missense	possibly damaging	0.69
R2219:Zfp735	UTSW	11	73,601,851 (GRCm39)	missense	possibly damaging	0.53
R2227:Zfp735	UTSW	11	73,602,223 (GRCm39)	nonsense	probably null
R2227:Zfp735	UTSW	11	73,602,222 (GRCm39)	missense	possibly damaging	0.53
R3552:Zfp735	UTSW	11	73,602,067 (GRCm39)	nonsense	probably null
R3856:Zfp735	UTSW	11	73,602,282 (GRCm39)	missense	probably benign	0.01
R3925:Zfp735	UTSW	11	73,601,950 (GRCm39)	missense	probably benign	0.33
R4572:Zfp735	UTSW	11	73,580,611 (GRCm39)	missense	probably benign	0.02
R4585:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4586:Zfp735	UTSW	11	73,580,550 (GRCm39)	missense	possibly damaging	0.51
R4619:Zfp735	UTSW	11	73,602,031 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,682 (GRCm39)	missense	probably damaging	0.98
R4687:Zfp735	UTSW	11	73,602,681 (GRCm39)	missense	probably damaging	0.98
R5435:Zfp735	UTSW	11	73,602,939 (GRCm39)	missense	possibly damaging	0.72
R5489:Zfp735	UTSW	11	73,601,419 (GRCm39)	nonsense	probably null
R5516:Zfp735	UTSW	11	73,601,640 (GRCm39)	missense	probably benign
R5654:Zfp735	UTSW	11	73,602,964 (GRCm39)	missense	possibly damaging	0.71
R5990:Zfp735	UTSW	11	73,581,174 (GRCm39)	missense	possibly damaging	0.70
R6332:Zfp735	UTSW	11	73,602,504 (GRCm39)	nonsense	probably null
R6427:Zfp735	UTSW	11	73,581,140 (GRCm39)	missense	possibly damaging	0.73
R6460:Zfp735	UTSW	11	73,602,478 (GRCm39)	missense	probably benign	0.33
R6831:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6833:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6834:Zfp735	UTSW	11	73,601,434 (GRCm39)	missense	probably damaging	1.00
R6897:Zfp735	UTSW	11	73,601,880 (GRCm39)	missense	probably benign	0.08
R6941:Zfp735	UTSW	11	73,581,159 (GRCm39)	missense	probably benign	0.33
R7335:Zfp735	UTSW	11	73,602,379 (GRCm39)	missense	possibly damaging	0.47
R7366:Zfp735	UTSW	11	73,602,979 (GRCm39)	missense	possibly damaging	0.93
R7474:Zfp735	UTSW	11	73,602,002 (GRCm39)	missense	possibly damaging	0.72
R7487:Zfp735	UTSW	11	73,581,154 (GRCm39)	missense	possibly damaging	0.53
R7583:Zfp735	UTSW	11	73,601,933 (GRCm39)	missense	possibly damaging	0.86
R7866:Zfp735	UTSW	11	73,601,629 (GRCm39)	missense	probably benign	0.00
R8005:Zfp735	UTSW	11	73,603,140 (GRCm39)	nonsense	probably null
R8500:Zfp735	UTSW	11	73,601,811 (GRCm39)	missense	possibly damaging	0.53
R8551:Zfp735	UTSW	11	73,603,122 (GRCm39)	missense	probably benign	0.06
R8754:Zfp735	UTSW	11	73,603,000 (GRCm39)	missense	possibly damaging	0.85
R8769:Zfp735	UTSW	11	73,581,127 (GRCm39)	missense	possibly damaging	0.53
R8794:Zfp735	UTSW	11	73,603,029 (GRCm39)	missense	possibly damaging	0.66
R8835:Zfp735	UTSW	11	73,601,692 (GRCm39)	missense	possibly damaging	0.53
R8869:Zfp735	UTSW	11	73,602,510 (GRCm39)	missense	possibly damaging	0.53
R8969:Zfp735	UTSW	11	73,602,699 (GRCm39)	missense	possibly damaging	0.83
R9072:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9073:Zfp735	UTSW	11	73,603,060 (GRCm39)	missense	probably benign	0.21
R9193:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	possibly damaging	0.71
R9355:Zfp735	UTSW	11	73,602,362 (GRCm39)	missense	probably benign	0.01
R9414:Zfp735	UTSW	11	73,602,023 (GRCm39)	nonsense	probably null
R9456:Zfp735	UTSW	11	73,602,403 (GRCm39)	missense	possibly damaging	0.53
R9573:Zfp735	UTSW	11	73,602,936 (GRCm39)	missense	possibly damaging	0.67
R9647:Zfp735	UTSW	11	73,580,600 (GRCm39)	missense	probably damaging	0.98
R9710:Zfp735	UTSW	11	73,601,806 (GRCm39)	missense	possibly damaging	0.86
Z1176:Zfp735	UTSW	11	73,601,641 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- ATCTGTTCCCTGGAGAAGGC -3'
(R):5'- CCTTTCTGCGAAGATAAATAGGC -3'

Sequencing Primer
(F):5'- AGAAGGCTTGTTAGAGATTCGATTCC -3'
(R):5'- GTGGTTTCCTAAGTCACCAGAAAGC -3'

Posted On

2018-10-18