|Institutional Source||Beutler Lab|
|Gene Name||cyclin T2|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R6821 (G1)|
|Chromosomal Location||127774164-127808061 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 127803335 bp|
|Amino Acid Change||Serine to Proline at position 650 (S650P)|
|Ref Sequence||ENSEMBL: ENSMUSP00000027587 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000027587] [ENSMUST00000112570]|
|Predicted Effect||probably damaging
AA Change: S650P
PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)
AA Change: S650P
|Predicted Effect||probably benign
|Meta Mutation Damage Score||0.1028|
|Coding Region Coverage||
|Validation Efficiency||97% (63/65)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. A pseudogene of this gene is found on chromosome 1. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]
PHENOTYPE: Mice homozygous for a gene trap allele die prior to the 4-cell stage. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Ccnt2||
(F):5'- GCCACAAGTATTTGCACATGC -3'
(R):5'- TGTAGTCCATATGCTGGCTG -3'
(F):5'- ACAGCGGATGGAATGCCTC -3'
(R):5'- CATATGCTGGCTGCTTGTACTGTAC -3'