|Institutional Source||Beutler Lab|
|Gene Name||tyrosyl-tRNA synthetase|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R6837 (G1)|
|Chromosomal Location||129189760-129219607 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to A at 129209751 bp|
|Amino Acid Change||Serine to Threonine at position 298 (S298T)|
|Ref Sequence||ENSEMBL: ENSMUSP00000101669 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000106054]|
|Predicted Effect||possibly damaging
AA Change: S298T
PolyPhen 2 Score 0.771 (Sensitivity: 0.85; Specificity: 0.92)
AA Change: S298T
|Predicted Effect||probably benign
|Coding Region Coverage||
|Validation Efficiency||98% (41/42)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Yars||
(F):5'- ATCTGGGTTACGTGCAGTCC -3'
(R):5'- AGTCTCACAGCACACATTTTG -3'
(F):5'- GTTACGTGCAGTCCTAGCTCAAAAG -3'
(R):5'- TTTGTATATGAAACCAGGGCAGCC -3'