Mutagenetix > Incidental Mutation

Incidental Mutation 'R6837:Vmn2r114'

537918

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r114

Ensembl Gene

ENSMUSG00000091945

Gene Name

vomeronasal 2, receptor 114

Synonyms

EG666002

MMRRC Submission

044945-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.142) question?

Stock #

R6837 (G1)

Quality Score

108.008

Status

Not validated

Chromosome

Chromosomal Location

23509908-23531287 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23529176 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 309 (M309V)

Ref Sequence

ENSEMBL: ENSMUSP00000127505 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168033]

AlphaFold

E9Q281

Predicted Effect

probably benign
Transcript: ENSMUST00000168033
AA Change: M309V

PolyPhen 2 Score 0.097 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000127505
Gene: ENSMUSG00000091945
AA Change: M309V

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	470	1.5e-24	PFAM
Pfam:NCD3G	511	564	1.5e-18	PFAM
Pfam:7tm_3	597	832	1.4e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

98% (41/42)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akap6	G	A	12: 53,188,045 (GRCm39)	E1820K	probably damaging	Het
Anxa8	A	T	14: 33,814,511 (GRCm39)	I116F	probably damaging	Het
Arid4a	A	G	12: 71,122,289 (GRCm39)	D890G	probably benign	Het
Aup1	C	T	6: 83,034,279 (GRCm39)	T396I	possibly damaging	Het
C2cd3	G	C	7: 100,097,953 (GRCm39)	E594Q	probably damaging	Het
Colgalt2	C	T	1: 152,382,579 (GRCm39)	P477L	probably damaging	Het
Dennd3	G	A	15: 73,429,542 (GRCm39)	D20N	probably damaging	Het
Dpysl3	A	G	18: 43,570,947 (GRCm39)	I109T	probably benign	Het
Fas	A	T	19: 34,284,564 (GRCm39)	T24S	probably damaging	Het
Fras1	A	T	5: 96,874,832 (GRCm39)	I2332F	probably damaging	Het
Gm10267	T	C	18: 44,291,375 (GRCm39)	H32R	probably benign	Het
Gm57859	A	G	11: 113,579,439 (GRCm39)	H278R	possibly damaging	Het
Hcfc2	A	T	10: 82,575,030 (GRCm39)	I230F	probably damaging	Het
Hdac9	T	C	12: 34,337,463 (GRCm39)	T673A	probably benign	Het
Herc2	T	G	7: 55,839,589 (GRCm39)	N3366K	possibly damaging	Het
Hhla1	T	C	15: 65,820,334 (GRCm39)	N139D	probably damaging	Het
Hip1r	A	G	5: 124,136,928 (GRCm39)	E632G	possibly damaging	Het
Kcnc2	A	G	10: 112,294,407 (GRCm39)	D98G	probably damaging	Het
Maip1	T	A	1: 57,454,891 (GRCm39)	*292K	probably null	Het
Map2	T	C	1: 66,453,731 (GRCm39)	F874L	probably damaging	Het
Myof	A	T	19: 37,911,404 (GRCm39)		probably null	Het
Notch2	A	G	3: 97,978,170 (GRCm39)		probably null	Het
Npy5r	T	A	8: 67,134,392 (GRCm39)	M134L	probably benign	Het
Ntsr2	T	A	12: 16,709,710 (GRCm39)	M225K	probably benign	Het
Nup153	G	A	13: 46,847,527 (GRCm39)	T634I	probably damaging	Het
Or51f1d	T	A	7: 102,700,929 (GRCm39)	Y141*	probably null	Het
Pcsk5	T	C	19: 17,416,448 (GRCm39)	S1667G	probably benign	Het
Pex13	A	G	11: 23,599,527 (GRCm39)	I328T	possibly damaging	Het
Pkd2	T	G	5: 104,624,909 (GRCm39)	L235W	probably damaging	Het
Prune2	A	T	19: 17,156,292 (GRCm39)	D66V	probably damaging	Het
Rasal3	T	C	17: 32,622,044 (GRCm39)	N105S	probably benign	Het
Sla	A	T	15: 66,658,939 (GRCm39)	I144N	probably damaging	Het
Slc5a4b	A	T	10: 75,898,220 (GRCm39)	I498N	possibly damaging	Het
Snx14	C	T	9: 88,262,276 (GRCm39)	E872K	probably benign	Het
Stx16	G	A	2: 173,935,795 (GRCm39)	R242H	probably benign	Het
Tg	T	C	15: 66,567,984 (GRCm39)	F1296S	probably damaging	Het
Tmem161b	C	A	13: 84,370,537 (GRCm39)		probably benign	Het
Tmf1	G	A	6: 97,153,542 (GRCm39)	T177M	possibly damaging	Het
Tuba4a	T	C	1: 75,194,038 (GRCm39)	Q14R	probably damaging	Het
Vps13c	C	T	9: 67,817,504 (GRCm39)	P1059S	probably benign	Het
Yars1	T	A	4: 129,103,544 (GRCm39)	S298T	possibly damaging	Het

Other mutations in Vmn2r114

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00157:Vmn2r114	APN	17	23,510,639 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,510,212 (GRCm39)	missense	probably damaging	1.00
IGL00990:Vmn2r114	APN	17	23,509,957 (GRCm39)	missense	probably benign	0.23
IGL00990:Vmn2r114	APN	17	23,509,939 (GRCm39)	missense	probably benign
IGL01838:Vmn2r114	APN	17	23,515,956 (GRCm39)	missense	probably benign	0.44
IGL01990:Vmn2r114	APN	17	23,529,355 (GRCm39)	missense	probably benign	0.22
IGL01994:Vmn2r114	APN	17	23,529,451 (GRCm39)	missense	probably damaging	1.00
IGL02153:Vmn2r114	APN	17	23,510,782 (GRCm39)	missense	probably benign	0.01
IGL02453:Vmn2r114	APN	17	23,530,108 (GRCm39)	missense	probably benign	0.00
IGL02621:Vmn2r114	APN	17	23,529,494 (GRCm39)	missense	probably damaging	0.98
IGL02938:Vmn2r114	APN	17	23,510,263 (GRCm39)	missense	probably benign	0.10
IGL03130:Vmn2r114	APN	17	23,515,970 (GRCm39)	splice site	probably benign
IGL03325:Vmn2r114	APN	17	23,510,652 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r114	UTSW	17	23,510,619 (GRCm39)	missense	probably damaging	1.00
R0109:Vmn2r114	UTSW	17	23,529,549 (GRCm39)	nonsense	probably null
R0164:Vmn2r114	UTSW	17	23,528,800 (GRCm39)	critical splice donor site	probably null
R0310:Vmn2r114	UTSW	17	23,509,917 (GRCm39)	missense	probably benign	0.23
R0583:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R0677:Vmn2r114	UTSW	17	23,529,568 (GRCm39)	missense	probably damaging	1.00
R1127:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1147:Vmn2r114	UTSW	17	23,530,037 (GRCm39)	missense	probably benign	0.00
R1157:Vmn2r114	UTSW	17	23,529,314 (GRCm39)	missense	possibly damaging	0.60
R1323:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1347:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1435:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R1437:Vmn2r114	UTSW	17	23,510,185 (GRCm39)	missense	probably damaging	1.00
R1585:Vmn2r114	UTSW	17	23,510,675 (GRCm39)	missense	probably damaging	0.98
R1641:Vmn2r114	UTSW	17	23,515,962 (GRCm39)	missense	probably benign	0.00
R1748:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably benign	0.17
R1954:Vmn2r114	UTSW	17	23,530,086 (GRCm39)	missense	probably benign	0.32
R2081:Vmn2r114	UTSW	17	23,510,083 (GRCm39)	missense	possibly damaging	0.91
R2103:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2113:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2134:Vmn2r114	UTSW	17	23,510,737 (GRCm39)	missense	probably damaging	1.00
R2149:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R2424:Vmn2r114	UTSW	17	23,515,842 (GRCm39)	missense	possibly damaging	0.90
R2847:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2848:Vmn2r114	UTSW	17	23,509,948 (GRCm39)	missense	probably benign	0.00
R2893:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3017:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3018:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3019:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3020:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R3021:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4628:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R4668:Vmn2r114	UTSW	17	23,529,447 (GRCm39)	missense	possibly damaging	0.83
R4840:Vmn2r114	UTSW	17	23,510,353 (GRCm39)	missense	probably damaging	0.97
R4841:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4842:Vmn2r114	UTSW	17	23,529,336 (GRCm39)	missense	probably benign	0.04
R4856:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4886:Vmn2r114	UTSW	17	23,527,008 (GRCm39)	missense	probably benign	0.11
R4992:Vmn2r114	UTSW	17	23,510,765 (GRCm39)	missense	probably benign	0.03
R5182:Vmn2r114	UTSW	17	23,510,632 (GRCm39)	missense	probably damaging	0.96
R5223:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5405:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5449:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5615:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null
R5834:Vmn2r114	UTSW	17	23,529,599 (GRCm39)	missense	possibly damaging	0.90
R6150:Vmn2r114	UTSW	17	23,510,269 (GRCm39)	missense	probably benign	0.03
R6277:Vmn2r114	UTSW	17	23,509,954 (GRCm39)	missense	possibly damaging	0.93
R6403:Vmn2r114	UTSW	17	23,528,939 (GRCm39)	missense	probably damaging	0.99
R6589:Vmn2r114	UTSW	17	23,510,642 (GRCm39)	missense	probably damaging	1.00
R6613:Vmn2r114	UTSW	17	23,529,220 (GRCm39)	missense	possibly damaging	0.82
R6747:Vmn2r114	UTSW	17	23,528,850 (GRCm39)	missense	probably benign	0.00
R6911:Vmn2r114	UTSW	17	23,510,104 (GRCm39)	missense	probably damaging	0.98
R6950:Vmn2r114	UTSW	17	23,529,137 (GRCm39)	missense	probably benign	0.03
R7276:Vmn2r114	UTSW	17	23,509,934 (GRCm39)	missense	probably damaging	0.97
R7482:Vmn2r114	UTSW	17	23,510,468 (GRCm39)	missense	probably damaging	1.00
R7514:Vmn2r114	UTSW	17	23,527,035 (GRCm39)	missense	probably null	0.96
R7523:Vmn2r114	UTSW	17	23,529,611 (GRCm39)	missense	probably benign	0.01
R7563:Vmn2r114	UTSW	17	23,510,000 (GRCm39)	missense	probably benign	0.01
R7585:Vmn2r114	UTSW	17	23,510,239 (GRCm39)	missense	probably damaging	1.00
R7593:Vmn2r114	UTSW	17	23,510,817 (GRCm39)	nonsense	probably null
R7611:Vmn2r114	UTSW	17	23,515,944 (GRCm39)	missense	probably damaging	0.97
R7641:Vmn2r114	UTSW	17	23,527,177 (GRCm39)	missense	possibly damaging	0.53
R7651:Vmn2r114	UTSW	17	23,509,986 (GRCm39)	nonsense	probably null
R7970:Vmn2r114	UTSW	17	23,530,186 (GRCm39)	missense	probably benign	0.00
R8737:Vmn2r114	UTSW	17	23,529,142 (GRCm39)	missense	probably benign	0.36
R8802:Vmn2r114	UTSW	17	23,528,836 (GRCm39)	missense	possibly damaging	0.65
R8847:Vmn2r114	UTSW	17	23,528,986 (GRCm39)	missense	probably damaging	1.00
R8991:Vmn2r114	UTSW	17	23,529,286 (GRCm39)	missense	probably damaging	1.00
R9138:Vmn2r114	UTSW	17	23,510,578 (GRCm39)	missense	probably damaging	1.00
R9173:Vmn2r114	UTSW	17	23,510,527 (GRCm39)	missense	probably damaging	0.99
R9175:Vmn2r114	UTSW	17	23,527,212 (GRCm39)	missense	probably damaging	1.00
R9657:Vmn2r114	UTSW	17	23,510,690 (GRCm39)	missense	probably damaging	1.00
R9670:Vmn2r114	UTSW	17	23,531,098 (GRCm39)	missense
X0065:Vmn2r114	UTSW	17	23,529,931 (GRCm39)	missense	probably benign	0.34
Z1088:Vmn2r114	UTSW	17	23,509,906 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- CATTTAGAGGCTGAGACCTCAC -3'
(R):5'- AAAATGCAGTCTGCTTTGCC -3'

Sequencing Primer
(F):5'- GCTGAGACCTCACAGTTAAAGTGC -3'
(R):5'- GCCTTTGTCAACATGATTCCAATGAG -3'

Posted On

2018-10-18