|Institutional Source||Beutler Lab|
|Gene Name||regulator of G-protein signalling 10|
|Is this an essential gene?||Probably non essential (E-score: 0.217)|
|Stock #||R6903 (G1)|
|Chromosomal Location||128373621-128418758 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to G at 128389073 bp|
|Amino Acid Change||Phenylalanine to Serine at position 97 (F97S)|
|Ref Sequence||ENSEMBL: ENSMUSP00000033133 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000033133]|
|Predicted Effect||probably damaging
AA Change: F97S
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: F97S
|Meta Mutation Damage Score||0.9211|
|Coding Region Coverage||
|Validation Efficiency||100% (46/46)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: A gene trap mutation of this gene results in impaired glucose tolerance and increased fasting glucose levels whereas a targeted knockout shows defects in osteoclast differentiation and in the skeleton. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Rgs10||
(F):5'- CTGAAGAATTCTCCCAGGCC -3'
(R):5'- GGCCTCAGAAGCTCTATTAGG -3'
(F):5'- CCAGGCCTGGGGAGAACATG -3'
(R):5'- CCTCAGAAGCTCTATTAGGTGTGG -3'