Incidental Mutation 'R6910:Try5'
ID 538975
Institutional Source Beutler Lab
Gene Symbol Try5
Ensembl Gene ENSMUSG00000036938
Gene Name trypsin 5
Synonyms 1810049H19Rik, Tc
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.070) question?
Stock # R6910 (G1)
Quality Score 225.009
Status Validated
Chromosome 6
Chromosomal Location 41311233-41316841 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to C at 41311799 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 54 (D54G)
Ref Sequence ENSEMBL: ENSMUSP00000133640 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000064324] [ENSMUST00000173916]
AlphaFold Q9QUK9
Predicted Effect possibly damaging
Transcript: ENSMUST00000064324
AA Change: D162G

PolyPhen 2 Score 0.623 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000064498
Gene: ENSMUSG00000036938
AA Change: D162G

DomainStartEndE-ValueType
low complexity region 4 16 N/A INTRINSIC
Tryp_SPc 23 239 1.47e-104 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000173916
AA Change: D54G

PolyPhen 2 Score 0.623 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000133640
Gene: ENSMUSG00000036938
AA Change: D54G

DomainStartEndE-ValueType
Tryp_SPc 1 131 1.21e-27 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.4%
  • 20x: 98.2%
Validation Efficiency 100% (37/37)
Allele List at MGI
Other mutations in this stock
Total: 36 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
0610010F05Rik A G 11: 23,620,447 V151A probably damaging Het
Cfap54 A G 10: 92,836,512 S2899P probably benign Het
Chil5 A G 3: 106,019,661 W82R probably damaging Het
Dennd3 A G 15: 73,555,116 T781A probably benign Het
Epha2 A G 4: 141,321,513 D597G probably damaging Het
Gcn1l1 A G 5: 115,606,538 T1598A probably benign Het
Glp2r A G 11: 67,730,671 F162S probably benign Het
Gm10130 A T 2: 150,324,067 Q56L probably benign Het
Gm17655 T A 5: 110,047,173 R248* probably null Het
Gm9268 T C 7: 43,024,051 F178L probably benign Het
Gnptab G A 10: 88,431,396 G450S probably damaging Het
Gpatch2l G A 12: 86,244,184 R47H probably damaging Het
Hapln2 A G 3: 88,023,828 Y127H probably damaging Het
Hdac4 G T 1: 91,982,153 T463K probably damaging Het
Ift80 A C 3: 68,927,735 S458A probably benign Het
Lama1 A G 17: 67,791,464 D1846G possibly damaging Het
Map3k8 A G 18: 4,340,801 I171T probably benign Het
Micu1 A G 10: 59,740,667 E115G probably damaging Het
Mrpl39 A G 16: 84,735,192 V9A unknown Het
Ncoa7 T G 10: 30,694,121 I281L possibly damaging Het
Nms A G 1: 38,941,895 E54G probably benign Het
Nrip1 G A 16: 76,294,417 A84V probably damaging Het
Olfr1331 T A 4: 118,869,138 M119K probably damaging Het
Olfr743 G A 14: 50,533,873 V154M probably benign Het
Pcdhga10 T A 18: 37,748,232 S349T probably damaging Het
R3hcc1 G A 14: 69,697,575 P454L probably damaging Het
Rsrc1 C T 3: 66,994,649 P44L unknown Het
Ryr3 T C 2: 112,958,175 D170G probably damaging Het
Scp2 C A 4: 108,105,086 G81C probably damaging Het
Sez6 A G 11: 77,953,869 T173A possibly damaging Het
Syne1 T C 10: 5,048,887 H8142R probably benign Het
Tcof1 G C 18: 60,829,051 A702G possibly damaging Het
Tnfrsf11a A G 1: 105,844,546 T520A probably damaging Het
Tpm1 A G 9: 67,031,974 S170P probably damaging Het
Zan T C 5: 137,419,080 E3041G unknown Het
Zfp616 A T 11: 74,085,002 H699L probably damaging Het
Other mutations in Try5
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01837:Try5 APN 6 41313424 missense probably benign 0.31
IGL01885:Try5 APN 6 41311738 missense possibly damaging 0.93
IGL02652:Try5 APN 6 41311408 missense probably benign 0.01
R1955:Try5 UTSW 6 41311769 missense probably benign 0.17
R2014:Try5 UTSW 6 41314651 splice site probably null
R2015:Try5 UTSW 6 41314651 splice site probably null
R2848:Try5 UTSW 6 41313476 missense probably benign 0.01
R4227:Try5 UTSW 6 41313467 missense possibly damaging 0.65
R4685:Try5 UTSW 6 41311299 missense possibly damaging 0.59
R4816:Try5 UTSW 6 41313415 missense probably benign 0.18
R5230:Try5 UTSW 6 41312378 missense probably benign 0.19
R5658:Try5 UTSW 6 41312427 missense probably damaging 1.00
R6518:Try5 UTSW 6 41314679 missense probably benign
R6913:Try5 UTSW 6 41311332 missense probably damaging 1.00
R7219:Try5 UTSW 6 41311703 missense probably damaging 1.00
R7242:Try5 UTSW 6 41313454 missense probably benign 0.09
R7444:Try5 UTSW 6 41311365 missense probably benign 0.00
R7575:Try5 UTSW 6 41311814 missense probably benign 0.05
R7585:Try5 UTSW 6 41311814 missense probably benign 0.14
R8011:Try5 UTSW 6 41313487 missense probably benign 0.12
R8739:Try5 UTSW 6 41311703 missense probably damaging 1.00
R8991:Try5 UTSW 6 41312361 missense probably benign 0.18
R9397:Try5 UTSW 6 41312380 missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- TGGGCATGTTAACCCCAATCC -3'
(R):5'- TTTGCAGACATGTAAACAGAGC -3'

Sequencing Primer
(F):5'- GGCATGTTAACCCCAATCCTAGGAG -3'
(R):5'- CTGTTATCACTTCTAGGAAGGACATC -3'
Posted On 2018-11-06