Incidental Mutation 'R6920:Traip'
ID539589
Institutional Source Beutler Lab
Gene Symbol Traip
Ensembl Gene ENSMUSG00000032586
Gene NameTRAF-interacting protein
SynonymsTrip
MMRRC Submission
Accession Numbers

NCBI RefSeq: NM_011634.3; MGI:1096377

Is this an essential gene? Essential (E-score: 1.000) question?
Stock #R6920 (G1)
Quality Score225.009
Status Validated
Chromosome9
Chromosomal Location107950136-107972270 bp(+) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) C to T at 107961041 bp
ZygosityHeterozygous
Amino Acid Change Arginine to Stop codon at position 142 (R142*)
Ref Sequence ENSEMBL: ENSMUSP00000141689 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000049348] [ENSMUST00000194271]
Predicted Effect probably null
Transcript: ENSMUST00000049348
AA Change: R158*
SMART Domains Protein: ENSMUSP00000040001
Gene: ENSMUSG00000032586
AA Change: R158*

DomainStartEndE-ValueType
RING 7 49 6.68e-6 SMART
coiled coil region 70 278 N/A INTRINSIC
Predicted Effect probably null
Transcript: ENSMUST00000194271
AA Change: R142*
SMART Domains Protein: ENSMUSP00000141689
Gene: ENSMUSG00000032586
AA Change: R142*

DomainStartEndE-ValueType
RING 7 49 3.2e-8 SMART
coiled coil region 54 143 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.1%
  • 20x: 97.2%
Validation Efficiency 100% (65/65)
MGI Phenotype Strain: 3764469
Lethality: E7-E9
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality at prior to E8.5, embryonic growth retardation, decreased embryonic size, decreased cell proliferation and increased apoptosis. [provided by MGI curators]
Allele List at MGI

All alleles(10) : Targeted(1) Gene trapped(9)

Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1110002E22Rik A T 3: 138,068,050 Y1000F probably damaging Het
1700018B08Rik A G 8: 121,535,421 probably null Het
Aadat T C 8: 60,529,433 F245L probably damaging Het
Adcy10 T G 1: 165,575,658 L1575W probably damaging Het
Anks4b A T 7: 120,183,008 T421S probably damaging Het
Anpep A T 7: 79,825,349 I155N probably damaging Het
Arnt T C 3: 95,490,621 F572L probably damaging Het
Brip1 G A 11: 86,148,536 Q391* probably null Het
Brpf3 C A 17: 28,823,996 H1004N probably benign Het
Cand2 T A 6: 115,791,289 V465D possibly damaging Het
Card10 G T 15: 78,802,409 Y69* probably null Het
Catsperd A T 17: 56,655,175 K450* probably null Het
Ccdc137 T C 11: 120,460,183 L137P probably damaging Het
Cic T C 7: 25,290,682 S1905P probably damaging Het
Csmd3 C T 15: 47,644,205 G2971S probably damaging Het
Dmxl2 A G 9: 54,472,212 Y183H probably damaging Het
Drosha T C 15: 12,834,310 Y167H unknown Het
E330034G19Rik A G 14: 24,308,242 K214R unknown Het
Fam180a T G 6: 35,313,830 I73L possibly damaging Het
Fbxo28 G T 1: 182,341,421 H51N probably benign Het
Gm12728 T G 4: 105,790,336 probably null Het
Gm973 T C 1: 59,552,461 C335R possibly damaging Het
Gpx8 A G 13: 113,043,236 V177A probably damaging Het
Hdlbp A G 1: 93,412,361 probably null Het
Htt T G 5: 34,877,100 Y1972D probably null Het
Igkv6-14 T C 6: 70,435,132 Y56C possibly damaging Het
Kcnma1 A G 14: 23,526,534 probably null Het
Klc1 T C 12: 111,787,585 S105P probably damaging Het
Klhl20 T C 1: 161,093,696 D63G possibly damaging Het
Lamc3 A G 2: 31,908,689 D469G probably damaging Het
Lrit1 G C 14: 37,060,095 V242L probably damaging Het
Mboat4 G A 8: 34,124,711 R434H probably benign Het
Mttp T C 3: 138,115,282 K270E possibly damaging Het
Muc5ac G A 7: 141,793,298 C337Y possibly damaging Het
Nars A G 18: 64,501,400 V484A probably damaging Het
Noxa1 G T 2: 25,091,832 probably null Het
Olfr297 A G 7: 86,527,314 T186A probably benign Het
Olfr830 T A 9: 18,875,525 L63H probably damaging Het
Osbpl7 G A 11: 97,050,758 G36S probably damaging Het
P4htm C T 9: 108,583,613 G220D probably benign Het
Pcdhga7 A G 18: 37,715,146 I69V probably benign Het
Pla2g4e C T 2: 120,185,314 E250K possibly damaging Het
Plcd4 A G 1: 74,565,835 probably benign Het
Ppfia3 C A 7: 45,358,807 G213V possibly damaging Het
Ppp1r1a A G 15: 103,533,086 S67P probably damaging Het
Prss43 T C 9: 110,828,612 F193S probably benign Het
Rfx1 A G 8: 84,095,488 Y872C probably damaging Het
Rhot1 T A 11: 80,242,095 N218K probably benign Het
Sall1 A G 8: 89,030,393 F1028L probably damaging Het
Siglec1 G A 2: 131,078,077 Q845* probably null Het
Slc38a9 C T 13: 112,701,526 T275I possibly damaging Het
Slc39a4 A T 15: 76,613,270 S481T probably damaging Het
Ssr1 A T 13: 37,986,022 N191K probably damaging Het
Tenm4 A T 7: 96,895,550 S2258C probably damaging Het
Tm7sf3 T G 6: 146,606,147 R472S possibly damaging Het
Tmprss11a G A 5: 86,428,635 T119M probably benign Het
Utrn T C 10: 12,750,470 N100D probably damaging Het
Vmn1r74 T C 7: 11,847,648 S292P probably benign Het
Vmn2r71 A T 7: 85,623,900 I641F probably damaging Het
Vmn2r9 T A 5: 108,849,046 Y119F possibly damaging Het
Vmn2r98 A G 17: 19,065,248 N110S probably damaging Het
Vwce A G 19: 10,664,693 T928A probably benign Het
Zfp608 T A 18: 54,988,265 K83N probably damaging Het
Zfp808 A G 13: 62,173,168 H737R probably benign Het
Zswim4 T C 8: 84,214,085 N795S probably benign Het
Other mutations in Traip
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00088:Traip APN 9 107970550 missense probably benign 0.15
IGL01457:Traip APN 9 107970472 missense probably benign 0.00
IGL01597:Traip APN 9 107955924 critical splice donor site probably null
IGL02197:Traip APN 9 107968737 missense possibly damaging 0.89
IGL03077:Traip APN 9 107962926 unclassified probably benign
IGL03226:Traip APN 9 107970993 missense probably damaging 0.97
P0016:Traip UTSW 9 107968656 missense possibly damaging 0.93
R1693:Traip UTSW 9 107970030 missense probably damaging 0.99
R2054:Traip UTSW 9 107962919 missense probably benign 0.08
R4396:Traip UTSW 9 107959487 missense probably benign 0.02
R4617:Traip UTSW 9 107970019 missense probably benign 0.00
R6151:Traip UTSW 9 107970619 critical splice donor site probably null
R6241:Traip UTSW 9 107968734 missense probably benign 0.33
R7177:Traip UTSW 9 107960985 missense possibly damaging 0.62
R7191:Traip UTSW 9 107970017 missense probably benign
R7504:Traip UTSW 9 107961544 missense probably benign 0.05
X0018:Traip UTSW 9 107961656 critical splice donor site probably null
Predicted Primers PCR Primer
(F):5'- GGGCTCTTGGCAGTTTATCTCC -3'
(R):5'- GGCAAGCCACCTATCCATAG -3'

Sequencing Primer
(F):5'- CCAGCACATAGGGGTTATCCTTG -3'
(R):5'- TATCCATAGGCAACAGAACAAGAAG -3'
Posted On2018-11-06