Mutagenetix > Incidental Mutation

Incidental Mutation 'R6920:Gpx8'

539601

Institutional Source

Beutler Lab

Gene Symbol

Gpx8

Ensembl Gene

ENSMUSG00000021760

Gene Name

glutathione peroxidase 8 (putative)

Synonyms

2310016C16Rik

MMRRC Submission

045040-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.469) question?

Stock #

R6920 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

113042753-113046410 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 113043236 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 177 (V177A)

Ref Sequence

ENSEMBL: ENSMUSP00000022282 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000022282] [ENSMUST00000109244] [ENSMUST00000180543] [ENSMUST00000181117] [ENSMUST00000181568] [ENSMUST00000231962]

AlphaFold

Q9D7B7

Predicted Effect

probably damaging
Transcript: ENSMUST00000022282
AA Change: V177A

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000022282
Gene: ENSMUSG00000021760
AA Change: V177A

Domain	Start	End	E-Value	Type
Pfam:AhpC-TSA	44	143	1.3e-8	PFAM
Pfam:GSHPx	47	155	5.3e-36	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000109244

SMART Domains

Protein: ENSMUSP00000104867
Gene: ENSMUSG00000078926

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	391	432	3.55e1	SMART
WD40	473	517	2.01e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000180543

Predicted Effect

probably benign
Transcript: ENSMUST00000181117

SMART Domains

Protein: ENSMUSP00000137915
Gene: ENSMUSG00000078926

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	391	432	3.55e1	SMART
WD40	473	513	1.78e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181568

SMART Domains

Protein: ENSMUSP00000137849
Gene: ENSMUSG00000078926

Domain	Start	End	E-Value	Type
low complexity region	85	97	N/A	INTRINSIC
WD40	218	257	6.6e1	SMART
WD40	266	301	5.75e-1	SMART
WD40	304	341	9.24e-1	SMART
WD40	345	383	9.02e-7	SMART
WD40	431	475	2.01e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000181741

Predicted Effect

unknown
Transcript: ENSMUST00000231962
AA Change: S90P

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 97.2%

Validation Efficiency

100% (65/65)

MGI Phenotype

PHENOTYPE: Male homozygous mice exhibit chronic active dermatitis, with epodermal hyperplasia and hyperkeratosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	T	3: 138,068,050 (GRCm38)	Y1000F	probably damaging	Het
1700018B08Rik	A	G	8: 121,535,421 (GRCm38)		probably null	Het
Aadat	T	C	8: 60,529,433 (GRCm38)	F245L	probably damaging	Het
Adcy10	T	G	1: 165,575,658 (GRCm38)	L1575W	probably damaging	Het
Anks4b	A	T	7: 120,183,008 (GRCm38)	T421S	probably damaging	Het
Anpep	A	T	7: 79,825,349 (GRCm38)	I155N	probably damaging	Het
Arnt	T	C	3: 95,490,621 (GRCm38)	F572L	probably damaging	Het
Brip1	G	A	11: 86,148,536 (GRCm38)	Q391*	probably null	Het
Brpf3	C	A	17: 28,823,996 (GRCm38)	H1004N	probably benign	Het
Cand2	T	A	6: 115,791,289 (GRCm38)	V465D	possibly damaging	Het
Card10	G	T	15: 78,802,409 (GRCm38)	Y69*	probably null	Het
Catsperd	A	T	17: 56,655,175 (GRCm38)	K450*	probably null	Het
Ccdc137	T	C	11: 120,460,183 (GRCm38)	L137P	probably damaging	Het
Cic	T	C	7: 25,290,682 (GRCm38)	S1905P	probably damaging	Het
Csmd3	C	T	15: 47,644,205 (GRCm38)	G2971S	probably damaging	Het
Dmxl2	A	G	9: 54,472,212 (GRCm38)	Y183H	probably damaging	Het
Drosha	T	C	15: 12,834,310 (GRCm38)	Y167H	unknown	Het
E330034G19Rik	A	G	14: 24,308,242 (GRCm38)	K214R	unknown	Het
Fam180a	T	G	6: 35,313,830 (GRCm38)	I73L	possibly damaging	Het
Fbxo28	G	T	1: 182,341,421 (GRCm38)	H51N	probably benign	Het
Gm12728	T	G	4: 105,790,336 (GRCm38)		probably null	Het
Gm973	T	C	1: 59,552,461 (GRCm38)	C335R	possibly damaging	Het
Hdlbp	A	G	1: 93,412,361 (GRCm38)		probably null	Het
Htt	T	G	5: 34,877,100 (GRCm38)	Y1972D	probably null	Het
Igkv6-14	T	C	6: 70,435,132 (GRCm38)	Y56C	possibly damaging	Het
Kcnma1	A	G	14: 23,526,534 (GRCm38)		probably null	Het
Klc1	T	C	12: 111,787,585 (GRCm38)	S105P	probably damaging	Het
Klhl20	T	C	1: 161,093,696 (GRCm38)	D63G	possibly damaging	Het
Lamc3	A	G	2: 31,908,689 (GRCm38)	D469G	probably damaging	Het
Lrit1	G	C	14: 37,060,095 (GRCm38)	V242L	probably damaging	Het
Mboat4	G	A	8: 34,124,711 (GRCm38)	R434H	probably benign	Het
Mttp	T	C	3: 138,115,282 (GRCm38)	K270E	possibly damaging	Het
Muc5ac	G	A	7: 141,793,298 (GRCm38)	C337Y	possibly damaging	Het
Nars1	A	G	18: 64,501,400 (GRCm38)	V484A	probably damaging	Het
Noxa1	G	T	2: 25,091,832 (GRCm38)		probably null	Het
Or14c45	A	G	7: 86,527,314 (GRCm38)	T186A	probably benign	Het
Or7g18	T	A	9: 18,875,525 (GRCm38)	L63H	probably damaging	Het
Osbpl7	G	A	11: 97,050,758 (GRCm38)	G36S	probably damaging	Het
P4htm	C	T	9: 108,583,613 (GRCm38)	G220D	probably benign	Het
Pcdhga7	A	G	18: 37,715,146 (GRCm38)	I69V	probably benign	Het
Pla2g4e	C	T	2: 120,185,314 (GRCm38)	E250K	possibly damaging	Het
Plcd4	A	G	1: 74,565,835 (GRCm38)		probably benign	Het
Ppfia3	C	A	7: 45,358,807 (GRCm38)	G213V	possibly damaging	Het
Ppp1r1a	A	G	15: 103,533,086 (GRCm38)	S67P	probably damaging	Het
Prss43	T	C	9: 110,828,612 (GRCm38)	F193S	probably benign	Het
Rfx1	A	G	8: 84,095,488 (GRCm38)	Y872C	probably damaging	Het
Rhot1	T	A	11: 80,242,095 (GRCm38)	N218K	probably benign	Het
Sall1	A	G	8: 89,030,393 (GRCm38)	F1028L	probably damaging	Het
Siglec1	G	A	2: 131,078,077 (GRCm38)	Q845*	probably null	Het
Slc38a9	C	T	13: 112,701,526 (GRCm38)	T275I	possibly damaging	Het
Slc39a4	A	T	15: 76,613,270 (GRCm38)	S481T	probably damaging	Het
Ssr1	A	T	13: 37,986,022 (GRCm38)	N191K	probably damaging	Het
Tenm4	A	T	7: 96,895,550 (GRCm38)	S2258C	probably damaging	Het
Tm7sf3	T	G	6: 146,606,147 (GRCm38)	R472S	possibly damaging	Het
Tmprss11a	G	A	5: 86,428,635 (GRCm38)	T119M	probably benign	Het
Traip	C	T	9: 107,961,041 (GRCm38)	R142*	probably null	Het
Utrn	T	C	10: 12,750,470 (GRCm38)	N100D	probably damaging	Het
Vmn1r74	T	C	7: 11,847,648 (GRCm38)	S292P	probably benign	Het
Vmn2r71	A	T	7: 85,623,900 (GRCm38)	I641F	probably damaging	Het
Vmn2r9	T	A	5: 108,849,046 (GRCm38)	Y119F	possibly damaging	Het
Vmn2r98	A	G	17: 19,065,248 (GRCm38)	N110S	probably damaging	Het
Vwce	A	G	19: 10,664,693 (GRCm38)	T928A	probably benign	Het
Zfp608	T	A	18: 54,988,265 (GRCm38)	K83N	probably damaging	Het
Zfp808	A	G	13: 62,173,168 (GRCm38)	H737R	probably benign	Het
Zswim4	T	C	8: 84,214,085 (GRCm38)	N795S	probably benign	Het

Other mutations in Gpx8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00703:Gpx8	APN	13	113,045,513 (GRCm38)	missense	possibly damaging	0.61
IGL01110:Gpx8	APN	13	113,045,684 (GRCm38)	missense	probably benign	0.06
IGL02190:Gpx8	APN	13	113,043,309 (GRCm38)	splice site	probably benign
IGL03085:Gpx8	APN	13	113,043,261 (GRCm38)	missense	probably damaging	1.00
IGL03369:Gpx8	APN	13	113,043,162 (GRCm38)	missense	probably damaging	0.97
R0597:Gpx8	UTSW	13	113,045,501 (GRCm38)	missense	possibly damaging	0.72
R1494:Gpx8	UTSW	13	113,045,615 (GRCm38)	missense	possibly damaging	0.95
R1906:Gpx8	UTSW	13	113,045,576 (GRCm38)	missense	probably damaging	1.00
R1941:Gpx8	UTSW	13	113,046,275 (GRCm38)	missense	probably damaging	1.00
R2174:Gpx8	UTSW	13	113,045,606 (GRCm38)	missense	probably benign	0.08
R4414:Gpx8	UTSW	13	113,043,148 (GRCm38)	missense	possibly damaging	0.69
R4860:Gpx8	UTSW	13	113,045,508 (GRCm38)	nonsense	probably null
R4860:Gpx8	UTSW	13	113,045,508 (GRCm38)	nonsense	probably null
R7689:Gpx8	UTSW	13	113,043,177 (GRCm38)	missense	probably benign	0.04
R7904:Gpx8	UTSW	13	113,045,501 (GRCm38)	missense	probably benign	0.14
R8849:Gpx8	UTSW	13	113,043,170 (GRCm38)	missense	probably benign	0.28
R9569:Gpx8	UTSW	13	113,045,591 (GRCm38)	missense	probably damaging	1.00
X0062:Gpx8	UTSW	13	113,043,175 (GRCm38)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- GGGACAGCCATTACATCTGAG -3'
(R):5'- ATTAAAGCTCTGACCTCTGCTC -3'

Sequencing Primer
(F):5'- CATCTGAGTAGTATAGTAAGATGGCC -3'
(R):5'- GACCTCTGCTCTCTGTGGTG -3'

Posted On

2018-11-06