Mutagenetix > Incidental Mutation

Incidental Mutation 'R6920:Zfp608'

539614

Institutional Source

Beutler Lab

Gene Symbol

Zfp608

Ensembl Gene

ENSMUSG00000052713

Gene Name

zinc finger protein 608

Synonyms

4932417D18Rik

MMRRC Submission

045040-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.908) question?

Stock #

R6920 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

54888045-54990180 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 54988265 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Asparagine at position 83 (K83N)

Ref Sequence

ENSEMBL: ENSMUSP00000068192 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064763]

AlphaFold

Q56A10

Predicted Effect

probably damaging
Transcript: ENSMUST00000064763
AA Change: K83N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000068192
Gene: ENSMUSG00000052713
AA Change: K83N

Domain	Start	End	E-Value	Type
low complexity region	51	61	N/A	INTRINSIC
low complexity region	150	184	N/A	INTRINSIC
low complexity region	214	234	N/A	INTRINSIC
low complexity region	252	266	N/A	INTRINSIC
low complexity region	309	326	N/A	INTRINSIC
low complexity region	428	445	N/A	INTRINSIC
low complexity region	459	471	N/A	INTRINSIC
ZnF_C2H2	552	577	1.86e0	SMART
low complexity region	624	636	N/A	INTRINSIC
low complexity region	706	718	N/A	INTRINSIC
low complexity region	734	773	N/A	INTRINSIC
low complexity region	807	822	N/A	INTRINSIC
low complexity region	833	846	N/A	INTRINSIC
low complexity region	934	956	N/A	INTRINSIC
coiled coil region	1028	1054	N/A	INTRINSIC
low complexity region	1182	1193	N/A	INTRINSIC
low complexity region	1255	1268	N/A	INTRINSIC
low complexity region	1439	1450	N/A	INTRINSIC
low complexity region	1458	1466	N/A	INTRINSIC
low complexity region	1486	1502	N/A	INTRINSIC

Meta Mutation Damage Score

0.0590

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 97.2%

Validation Efficiency

100% (65/65)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	T	3: 138,068,050 (GRCm38)	Y1000F	probably damaging	Het
1700018B08Rik	A	G	8: 121,535,421 (GRCm38)		probably null	Het
Aadat	T	C	8: 60,529,433 (GRCm38)	F245L	probably damaging	Het
Adcy10	T	G	1: 165,575,658 (GRCm38)	L1575W	probably damaging	Het
Anks4b	A	T	7: 120,183,008 (GRCm38)	T421S	probably damaging	Het
Anpep	A	T	7: 79,825,349 (GRCm38)	I155N	probably damaging	Het
Arnt	T	C	3: 95,490,621 (GRCm38)	F572L	probably damaging	Het
Brip1	G	A	11: 86,148,536 (GRCm38)	Q391*	probably null	Het
Brpf3	C	A	17: 28,823,996 (GRCm38)	H1004N	probably benign	Het
Cand2	T	A	6: 115,791,289 (GRCm38)	V465D	possibly damaging	Het
Card10	G	T	15: 78,802,409 (GRCm38)	Y69*	probably null	Het
Catsperd	A	T	17: 56,655,175 (GRCm38)	K450*	probably null	Het
Ccdc137	T	C	11: 120,460,183 (GRCm38)	L137P	probably damaging	Het
Cic	T	C	7: 25,290,682 (GRCm38)	S1905P	probably damaging	Het
Csmd3	C	T	15: 47,644,205 (GRCm38)	G2971S	probably damaging	Het
Dmxl2	A	G	9: 54,472,212 (GRCm38)	Y183H	probably damaging	Het
Drosha	T	C	15: 12,834,310 (GRCm38)	Y167H	unknown	Het
E330034G19Rik	A	G	14: 24,308,242 (GRCm38)	K214R	unknown	Het
Fam180a	T	G	6: 35,313,830 (GRCm38)	I73L	possibly damaging	Het
Fbxo28	G	T	1: 182,341,421 (GRCm38)	H51N	probably benign	Het
Gm12728	T	G	4: 105,790,336 (GRCm38)		probably null	Het
Gm973	T	C	1: 59,552,461 (GRCm38)	C335R	possibly damaging	Het
Gpx8	A	G	13: 113,043,236 (GRCm38)	V177A	probably damaging	Het
Hdlbp	A	G	1: 93,412,361 (GRCm38)		probably null	Het
Htt	T	G	5: 34,877,100 (GRCm38)	Y1972D	probably null	Het
Igkv6-14	T	C	6: 70,435,132 (GRCm38)	Y56C	possibly damaging	Het
Kcnma1	A	G	14: 23,526,534 (GRCm38)		probably null	Het
Klc1	T	C	12: 111,787,585 (GRCm38)	S105P	probably damaging	Het
Klhl20	T	C	1: 161,093,696 (GRCm38)	D63G	possibly damaging	Het
Lamc3	A	G	2: 31,908,689 (GRCm38)	D469G	probably damaging	Het
Lrit1	G	C	14: 37,060,095 (GRCm38)	V242L	probably damaging	Het
Mboat4	G	A	8: 34,124,711 (GRCm38)	R434H	probably benign	Het
Mttp	T	C	3: 138,115,282 (GRCm38)	K270E	possibly damaging	Het
Muc5ac	G	A	7: 141,793,298 (GRCm38)	C337Y	possibly damaging	Het
Nars	A	G	18: 64,501,400 (GRCm38)	V484A	probably damaging	Het
Noxa1	G	T	2: 25,091,832 (GRCm38)		probably null	Het
Olfr297	A	G	7: 86,527,314 (GRCm38)	T186A	probably benign	Het
Olfr830	T	A	9: 18,875,525 (GRCm38)	L63H	probably damaging	Het
Osbpl7	G	A	11: 97,050,758 (GRCm38)	G36S	probably damaging	Het
P4htm	C	T	9: 108,583,613 (GRCm38)	G220D	probably benign	Het
Pcdhga7	A	G	18: 37,715,146 (GRCm38)	I69V	probably benign	Het
Pla2g4e	C	T	2: 120,185,314 (GRCm38)	E250K	possibly damaging	Het
Plcd4	A	G	1: 74,565,835 (GRCm38)		probably benign	Het
Ppfia3	C	A	7: 45,358,807 (GRCm38)	G213V	possibly damaging	Het
Ppp1r1a	A	G	15: 103,533,086 (GRCm38)	S67P	probably damaging	Het
Prss43	T	C	9: 110,828,612 (GRCm38)	F193S	probably benign	Het
Rfx1	A	G	8: 84,095,488 (GRCm38)	Y872C	probably damaging	Het
Rhot1	T	A	11: 80,242,095 (GRCm38)	N218K	probably benign	Het
Sall1	A	G	8: 89,030,393 (GRCm38)	F1028L	probably damaging	Het
Siglec1	G	A	2: 131,078,077 (GRCm38)	Q845*	probably null	Het
Slc38a9	C	T	13: 112,701,526 (GRCm38)	T275I	possibly damaging	Het
Slc39a4	A	T	15: 76,613,270 (GRCm38)	S481T	probably damaging	Het
Ssr1	A	T	13: 37,986,022 (GRCm38)	N191K	probably damaging	Het
Tenm4	A	T	7: 96,895,550 (GRCm38)	S2258C	probably damaging	Het
Tm7sf3	T	G	6: 146,606,147 (GRCm38)	R472S	possibly damaging	Het
Tmprss11a	G	A	5: 86,428,635 (GRCm38)	T119M	probably benign	Het
Traip	C	T	9: 107,961,041 (GRCm38)	R142*	probably null	Het
Utrn	T	C	10: 12,750,470 (GRCm38)	N100D	probably damaging	Het
Vmn1r74	T	C	7: 11,847,648 (GRCm38)	S292P	probably benign	Het
Vmn2r71	A	T	7: 85,623,900 (GRCm38)	I641F	probably damaging	Het
Vmn2r9	T	A	5: 108,849,046 (GRCm38)	Y119F	possibly damaging	Het
Vmn2r98	A	G	17: 19,065,248 (GRCm38)	N110S	probably damaging	Het
Vwce	A	G	19: 10,664,693 (GRCm38)	T928A	probably benign	Het
Zfp808	A	G	13: 62,173,168 (GRCm38)	H737R	probably benign	Het
Zswim4	T	C	8: 84,214,085 (GRCm38)	N795S	probably benign	Het

Other mutations in Zfp608

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00500:Zfp608	APN	18	54,988,333 (GRCm38)	missense	probably benign
IGL00920:Zfp608	APN	18	54,889,831 (GRCm38)	missense	probably benign	0.00
IGL01088:Zfp608	APN	18	54,898,087 (GRCm38)	missense	probably benign	0.03
IGL01447:Zfp608	APN	18	54,899,011 (GRCm38)	missense	possibly damaging	0.75
IGL01534:Zfp608	APN	18	54,898,932 (GRCm38)	missense	probably damaging	0.99
IGL01547:Zfp608	APN	18	54,894,449 (GRCm38)	critical splice donor site	probably null
IGL01933:Zfp608	APN	18	54,987,799 (GRCm38)	missense	probably benign
IGL01998:Zfp608	APN	18	54,891,818 (GRCm38)	missense	probably damaging	0.99
IGL02167:Zfp608	APN	18	54,988,224 (GRCm38)	missense	probably damaging	1.00
IGL02266:Zfp608	APN	18	54,897,581 (GRCm38)	missense	probably benign	0.08
IGL02335:Zfp608	APN	18	54,897,437 (GRCm38)	nonsense	probably null
IGL02455:Zfp608	APN	18	54,899,333 (GRCm38)	missense	probably damaging	1.00
IGL02612:Zfp608	APN	18	54,898,201 (GRCm38)	missense	probably damaging	1.00
IGL02900:Zfp608	APN	18	54,946,793 (GRCm38)	missense	probably damaging	1.00
PIT4243001:Zfp608	UTSW	18	54,898,024 (GRCm38)	missense	probably damaging	1.00
PIT4519001:Zfp608	UTSW	18	54,946,711 (GRCm38)	missense	possibly damaging	0.71
R0005:Zfp608	UTSW	18	54,895,520 (GRCm38)	missense	possibly damaging	0.88
R0010:Zfp608	UTSW	18	54,895,214 (GRCm38)	splice site	probably benign
R0010:Zfp608	UTSW	18	54,895,214 (GRCm38)	splice site	probably benign
R0064:Zfp608	UTSW	18	54,898,816 (GRCm38)	missense	probably benign
R0401:Zfp608	UTSW	18	54,898,994 (GRCm38)	missense	probably benign
R0722:Zfp608	UTSW	18	54,900,234 (GRCm38)	missense	probably damaging	1.00
R1351:Zfp608	UTSW	18	54,898,391 (GRCm38)	missense	probably benign	0.00
R1512:Zfp608	UTSW	18	54,946,666 (GRCm38)	missense	probably damaging	1.00
R1554:Zfp608	UTSW	18	54,898,054 (GRCm38)	missense	probably damaging	1.00
R1622:Zfp608	UTSW	18	54,988,294 (GRCm38)	missense	probably benign	0.07
R1669:Zfp608	UTSW	18	54,987,739 (GRCm38)	missense	probably benign	0.09
R1690:Zfp608	UTSW	18	54,987,634 (GRCm38)	missense	possibly damaging	0.62
R1721:Zfp608	UTSW	18	54,899,249 (GRCm38)	missense	probably benign
R1826:Zfp608	UTSW	18	54,898,576 (GRCm38)	missense	probably benign	0.03
R1864:Zfp608	UTSW	18	54,897,911 (GRCm38)	missense	probably benign	0.00
R1952:Zfp608	UTSW	18	54,897,779 (GRCm38)	nonsense	probably null
R2049:Zfp608	UTSW	18	54,895,565 (GRCm38)	missense	probably damaging	1.00
R2051:Zfp608	UTSW	18	54,988,314 (GRCm38)	missense	probably benign
R2168:Zfp608	UTSW	18	54,898,053 (GRCm38)	nonsense	probably null
R2218:Zfp608	UTSW	18	54,987,684 (GRCm38)	missense	probably benign	0.14
R2283:Zfp608	UTSW	18	54,988,374 (GRCm38)	missense	probably damaging	1.00
R2399:Zfp608	UTSW	18	54,897,902 (GRCm38)	missense	probably damaging	1.00
R2520:Zfp608	UTSW	18	54,988,506 (GRCm38)	missense	possibly damaging	0.92
R2961:Zfp608	UTSW	18	54,898,472 (GRCm38)	missense	possibly damaging	0.60
R4074:Zfp608	UTSW	18	54,898,108 (GRCm38)	missense	probably damaging	1.00
R4076:Zfp608	UTSW	18	54,898,108 (GRCm38)	missense	probably damaging	1.00
R4206:Zfp608	UTSW	18	54,988,195 (GRCm38)	missense	probably damaging	1.00
R4756:Zfp608	UTSW	18	54,894,472 (GRCm38)	missense	probably damaging	1.00
R4771:Zfp608	UTSW	18	54,988,300 (GRCm38)	missense	probably benign
R4820:Zfp608	UTSW	18	54,987,684 (GRCm38)	missense	probably benign	0.14
R4825:Zfp608	UTSW	18	54,897,969 (GRCm38)	missense	probably benign	0.01
R4912:Zfp608	UTSW	18	54,946,591 (GRCm38)	missense	probably damaging	1.00
R4975:Zfp608	UTSW	18	54,889,890 (GRCm38)	missense	probably damaging	1.00
R5138:Zfp608	UTSW	18	54,891,799 (GRCm38)	missense	probably damaging	1.00
R5192:Zfp608	UTSW	18	54,898,497 (GRCm38)	missense	probably damaging	1.00
R5557:Zfp608	UTSW	18	54,987,870 (GRCm38)	missense	possibly damaging	0.81
R5624:Zfp608	UTSW	18	54,898,272 (GRCm38)	missense	probably damaging	1.00
R5818:Zfp608	UTSW	18	54,895,396 (GRCm38)	missense	probably benign	0.02
R5840:Zfp608	UTSW	18	54,898,906 (GRCm38)	missense	probably damaging	1.00
R5992:Zfp608	UTSW	18	54,899,248 (GRCm38)	missense	probably benign	0.15
R6106:Zfp608	UTSW	18	54,987,872 (GRCm38)	missense	possibly damaging	0.54
R6174:Zfp608	UTSW	18	54,988,544 (GRCm38)	start gained	probably benign
R6181:Zfp608	UTSW	18	54,895,628 (GRCm38)	missense	possibly damaging	0.56
R6517:Zfp608	UTSW	18	54,899,078 (GRCm38)	missense	possibly damaging	0.94
R6567:Zfp608	UTSW	18	54,897,556 (GRCm38)	missense	probably damaging	1.00
R6668:Zfp608	UTSW	18	54,898,019 (GRCm38)	missense	probably damaging	1.00
R7061:Zfp608	UTSW	18	54,987,997 (GRCm38)	missense	probably benign
R7074:Zfp608	UTSW	18	54,897,382 (GRCm38)	missense	possibly damaging	0.69
R7087:Zfp608	UTSW	18	54,899,397 (GRCm38)	missense	probably damaging	1.00
R7391:Zfp608	UTSW	18	54,897,547 (GRCm38)	missense	possibly damaging	0.49
R7600:Zfp608	UTSW	18	54,988,020 (GRCm38)	missense	probably damaging	1.00
R7723:Zfp608	UTSW	18	54,897,601 (GRCm38)	missense	probably damaging	1.00
R8054:Zfp608	UTSW	18	54,899,546 (GRCm38)	missense	probably benign	0.28
R8236:Zfp608	UTSW	18	54,899,209 (GRCm38)	missense	probably damaging	1.00
R8260:Zfp608	UTSW	18	54,897,749 (GRCm38)	missense	possibly damaging	0.60
R8732:Zfp608	UTSW	18	54,988,000 (GRCm38)	missense	probably benign	0.06
R8781:Zfp608	UTSW	18	54,898,729 (GRCm38)	missense	probably damaging	1.00
R8851:Zfp608	UTSW	18	54,899,122 (GRCm38)	missense	possibly damaging	0.94
R8940:Zfp608	UTSW	18	54,900,229 (GRCm38)	missense	possibly damaging	0.93
R9051:Zfp608	UTSW	18	54,899,194 (GRCm38)	missense	probably damaging	1.00
R9091:Zfp608	UTSW	18	54,899,118 (GRCm38)	missense	probably damaging	1.00
R9092:Zfp608	UTSW	18	54,898,576 (GRCm38)	missense	probably benign	0.03
R9236:Zfp608	UTSW	18	54,899,282 (GRCm38)	missense	probably benign	0.39
R9270:Zfp608	UTSW	18	54,899,118 (GRCm38)	missense	probably damaging	1.00
R9283:Zfp608	UTSW	18	54,897,841 (GRCm38)	missense	possibly damaging	0.50
R9288:Zfp608	UTSW	18	54,900,269 (GRCm38)	missense	probably benign	0.39
R9463:Zfp608	UTSW	18	54,897,202 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCCAGCGCTGAATTCATG -3'
(R):5'- ATGACAGTGGCGATGATTGG -3'

Sequencing Primer
(F):5'- GCTGAATTCATGCCAGTAGC -3'
(R):5'- CAGTGGCGATGATTGGGAAATC -3'

Posted On

2018-11-06