Mutagenetix > Incidental Mutation

Incidental Mutation 'R6920:Zfp608'

539614

Institutional Source

Beutler Lab

Gene Symbol

Zfp608

Ensembl Gene

ENSMUSG00000052713

Gene Name

zinc finger protein 608

Synonyms

4932417D18Rik

MMRRC Submission

045040-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.917) question?

Stock #

R6920 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

55021120-55125627 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 55121337 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Asparagine at position 83 (K83N)

Ref Sequence

ENSEMBL: ENSMUSP00000068192 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000064763]

AlphaFold

Q56A10

Predicted Effect

probably damaging
Transcript: ENSMUST00000064763
AA Change: K83N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000068192
Gene: ENSMUSG00000052713
AA Change: K83N

Domain	Start	End	E-Value	Type
low complexity region	51	61	N/A	INTRINSIC
low complexity region	150	184	N/A	INTRINSIC
low complexity region	214	234	N/A	INTRINSIC
low complexity region	252	266	N/A	INTRINSIC
low complexity region	309	326	N/A	INTRINSIC
low complexity region	428	445	N/A	INTRINSIC
low complexity region	459	471	N/A	INTRINSIC
ZnF_C2H2	552	577	1.86e0	SMART
low complexity region	624	636	N/A	INTRINSIC
low complexity region	706	718	N/A	INTRINSIC
low complexity region	734	773	N/A	INTRINSIC
low complexity region	807	822	N/A	INTRINSIC
low complexity region	833	846	N/A	INTRINSIC
low complexity region	934	956	N/A	INTRINSIC
coiled coil region	1028	1054	N/A	INTRINSIC
low complexity region	1182	1193	N/A	INTRINSIC
low complexity region	1255	1268	N/A	INTRINSIC
low complexity region	1439	1450	N/A	INTRINSIC
low complexity region	1458	1466	N/A	INTRINSIC
low complexity region	1486	1502	N/A	INTRINSIC

Meta Mutation Damage Score

0.0590

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 97.2%

Validation Efficiency

100% (65/65)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	T	3: 137,773,811 (GRCm39)	Y1000F	probably damaging	Het
1700018B08Rik	A	G	8: 122,262,160 (GRCm39)		probably null	Het
Aadat	T	C	8: 60,982,467 (GRCm39)	F245L	probably damaging	Het
Adcy10	T	G	1: 165,403,227 (GRCm39)	L1575W	probably damaging	Het
Anks4b	A	T	7: 119,782,231 (GRCm39)	T421S	probably damaging	Het
Anpep	A	T	7: 79,475,097 (GRCm39)	I155N	probably damaging	Het
Arnt	T	C	3: 95,397,932 (GRCm39)	F572L	probably damaging	Het
Brip1	G	A	11: 86,039,362 (GRCm39)	Q391*	probably null	Het
Brpf3	C	A	17: 29,042,970 (GRCm39)	H1004N	probably benign	Het
Cand2	T	A	6: 115,768,250 (GRCm39)	V465D	possibly damaging	Het
Card10	G	T	15: 78,686,609 (GRCm39)	Y69*	probably null	Het
Catsperd	A	T	17: 56,962,175 (GRCm39)	K450*	probably null	Het
Ccdc137	T	C	11: 120,351,009 (GRCm39)	L137P	probably damaging	Het
Cic	T	C	7: 24,990,107 (GRCm39)	S1905P	probably damaging	Het
Csmd3	C	T	15: 47,507,601 (GRCm39)	G2971S	probably damaging	Het
Dmxl2	A	G	9: 54,379,496 (GRCm39)	Y183H	probably damaging	Het
Drosha	T	C	15: 12,834,396 (GRCm39)	Y167H	unknown	Het
E330034G19Rik	A	G	14: 24,358,310 (GRCm39)	K214R	unknown	Het
Fam180a	T	G	6: 35,290,765 (GRCm39)	I73L	possibly damaging	Het
Fbxo28	G	T	1: 182,168,986 (GRCm39)	H51N	probably benign	Het
Gm12728	T	G	4: 105,647,533 (GRCm39)		probably null	Het
Gm973	T	C	1: 59,591,620 (GRCm39)	C335R	possibly damaging	Het
Gpx8	A	G	13: 113,179,770 (GRCm39)	V177A	probably damaging	Het
Hdlbp	A	G	1: 93,340,083 (GRCm39)		probably null	Het
Htt	T	G	5: 35,034,444 (GRCm39)	Y1972D	probably null	Het
Igkv6-14	T	C	6: 70,412,116 (GRCm39)	Y56C	possibly damaging	Het
Kcnma1	A	G	14: 23,576,602 (GRCm39)		probably null	Het
Klc1	T	C	12: 111,754,019 (GRCm39)	S105P	probably damaging	Het
Klhl20	T	C	1: 160,921,266 (GRCm39)	D63G	possibly damaging	Het
Lamc3	A	G	2: 31,798,701 (GRCm39)	D469G	probably damaging	Het
Lrit1	G	C	14: 36,782,052 (GRCm39)	V242L	probably damaging	Het
Mboat4	G	A	8: 34,591,865 (GRCm39)	R434H	probably benign	Het
Mttp	T	C	3: 137,821,043 (GRCm39)	K270E	possibly damaging	Het
Muc5ac	G	A	7: 141,347,035 (GRCm39)	C337Y	possibly damaging	Het
Nars1	A	G	18: 64,634,471 (GRCm39)	V484A	probably damaging	Het
Noxa1	G	T	2: 24,981,844 (GRCm39)		probably null	Het
Or14c45	A	G	7: 86,176,522 (GRCm39)	T186A	probably benign	Het
Or7g18	T	A	9: 18,786,821 (GRCm39)	L63H	probably damaging	Het
Osbpl7	G	A	11: 96,941,584 (GRCm39)	G36S	probably damaging	Het
P4htm	C	T	9: 108,460,812 (GRCm39)	G220D	probably benign	Het
Pcdhga7	A	G	18: 37,848,199 (GRCm39)	I69V	probably benign	Het
Pla2g4e	C	T	2: 120,015,795 (GRCm39)	E250K	possibly damaging	Het
Plcd4	A	G	1: 74,604,994 (GRCm39)		probably benign	Het
Ppfia3	C	A	7: 45,008,231 (GRCm39)	G213V	possibly damaging	Het
Ppp1r1a	A	G	15: 103,441,513 (GRCm39)	S67P	probably damaging	Het
Prss43	T	C	9: 110,657,680 (GRCm39)	F193S	probably benign	Het
Rfx1	A	G	8: 84,822,117 (GRCm39)	Y872C	probably damaging	Het
Rhot1	T	A	11: 80,132,921 (GRCm39)	N218K	probably benign	Het
Sall1	A	G	8: 89,757,021 (GRCm39)	F1028L	probably damaging	Het
Siglec1	G	A	2: 130,919,997 (GRCm39)	Q845*	probably null	Het
Slc38a9	C	T	13: 112,838,060 (GRCm39)	T275I	possibly damaging	Het
Slc39a4	A	T	15: 76,497,470 (GRCm39)	S481T	probably damaging	Het
Ssr1	A	T	13: 38,169,998 (GRCm39)	N191K	probably damaging	Het
Tenm4	A	T	7: 96,544,757 (GRCm39)	S2258C	probably damaging	Het
Tm7sf3	T	G	6: 146,507,645 (GRCm39)	R472S	possibly damaging	Het
Tmprss11a	G	A	5: 86,576,494 (GRCm39)	T119M	probably benign	Het
Traip	C	T	9: 107,838,240 (GRCm39)	R142*	probably null	Het
Utrn	T	C	10: 12,626,214 (GRCm39)	N100D	probably damaging	Het
Vmn1r74	T	C	7: 11,581,575 (GRCm39)	S292P	probably benign	Het
Vmn2r71	A	T	7: 85,273,108 (GRCm39)	I641F	probably damaging	Het
Vmn2r9	T	A	5: 108,996,912 (GRCm39)	Y119F	possibly damaging	Het
Vmn2r98	A	G	17: 19,285,510 (GRCm39)	N110S	probably damaging	Het
Vwce	A	G	19: 10,642,057 (GRCm39)	T928A	probably benign	Het
Zfp808	A	G	13: 62,320,982 (GRCm39)	H737R	probably benign	Het
Zswim4	T	C	8: 84,940,714 (GRCm39)	N795S	probably benign	Het

Other mutations in Zfp608

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00500:Zfp608	APN	18	55,121,405 (GRCm39)	missense	probably benign
IGL00920:Zfp608	APN	18	55,022,903 (GRCm39)	missense	probably benign	0.00
IGL01088:Zfp608	APN	18	55,031,159 (GRCm39)	missense	probably benign	0.03
IGL01447:Zfp608	APN	18	55,032,083 (GRCm39)	missense	possibly damaging	0.75
IGL01534:Zfp608	APN	18	55,032,004 (GRCm39)	missense	probably damaging	0.99
IGL01547:Zfp608	APN	18	55,027,521 (GRCm39)	critical splice donor site	probably null
IGL01933:Zfp608	APN	18	55,120,871 (GRCm39)	missense	probably benign
IGL01998:Zfp608	APN	18	55,024,890 (GRCm39)	missense	probably damaging	0.99
IGL02167:Zfp608	APN	18	55,121,296 (GRCm39)	missense	probably damaging	1.00
IGL02266:Zfp608	APN	18	55,030,653 (GRCm39)	missense	probably benign	0.08
IGL02335:Zfp608	APN	18	55,030,509 (GRCm39)	nonsense	probably null
IGL02455:Zfp608	APN	18	55,032,405 (GRCm39)	missense	probably damaging	1.00
IGL02612:Zfp608	APN	18	55,031,273 (GRCm39)	missense	probably damaging	1.00
IGL02900:Zfp608	APN	18	55,079,865 (GRCm39)	missense	probably damaging	1.00
PIT4243001:Zfp608	UTSW	18	55,031,096 (GRCm39)	missense	probably damaging	1.00
PIT4519001:Zfp608	UTSW	18	55,079,783 (GRCm39)	missense	possibly damaging	0.71
R0005:Zfp608	UTSW	18	55,028,592 (GRCm39)	missense	possibly damaging	0.88
R0010:Zfp608	UTSW	18	55,028,286 (GRCm39)	splice site	probably benign
R0010:Zfp608	UTSW	18	55,028,286 (GRCm39)	splice site	probably benign
R0064:Zfp608	UTSW	18	55,031,888 (GRCm39)	missense	probably benign
R0401:Zfp608	UTSW	18	55,032,066 (GRCm39)	missense	probably benign
R0722:Zfp608	UTSW	18	55,033,306 (GRCm39)	missense	probably damaging	1.00
R1351:Zfp608	UTSW	18	55,031,463 (GRCm39)	missense	probably benign	0.00
R1512:Zfp608	UTSW	18	55,079,738 (GRCm39)	missense	probably damaging	1.00
R1554:Zfp608	UTSW	18	55,031,126 (GRCm39)	missense	probably damaging	1.00
R1622:Zfp608	UTSW	18	55,121,366 (GRCm39)	missense	probably benign	0.07
R1669:Zfp608	UTSW	18	55,120,811 (GRCm39)	missense	probably benign	0.09
R1690:Zfp608	UTSW	18	55,120,706 (GRCm39)	missense	possibly damaging	0.62
R1721:Zfp608	UTSW	18	55,032,321 (GRCm39)	missense	probably benign
R1826:Zfp608	UTSW	18	55,031,648 (GRCm39)	missense	probably benign	0.03
R1864:Zfp608	UTSW	18	55,030,983 (GRCm39)	missense	probably benign	0.00
R1952:Zfp608	UTSW	18	55,030,851 (GRCm39)	nonsense	probably null
R2049:Zfp608	UTSW	18	55,028,637 (GRCm39)	missense	probably damaging	1.00
R2051:Zfp608	UTSW	18	55,121,386 (GRCm39)	missense	probably benign
R2168:Zfp608	UTSW	18	55,031,125 (GRCm39)	nonsense	probably null
R2218:Zfp608	UTSW	18	55,120,756 (GRCm39)	missense	probably benign	0.14
R2283:Zfp608	UTSW	18	55,121,446 (GRCm39)	missense	probably damaging	1.00
R2399:Zfp608	UTSW	18	55,030,974 (GRCm39)	missense	probably damaging	1.00
R2520:Zfp608	UTSW	18	55,121,578 (GRCm39)	missense	possibly damaging	0.92
R2961:Zfp608	UTSW	18	55,031,544 (GRCm39)	missense	possibly damaging	0.60
R4074:Zfp608	UTSW	18	55,031,180 (GRCm39)	missense	probably damaging	1.00
R4076:Zfp608	UTSW	18	55,031,180 (GRCm39)	missense	probably damaging	1.00
R4206:Zfp608	UTSW	18	55,121,267 (GRCm39)	missense	probably damaging	1.00
R4756:Zfp608	UTSW	18	55,027,544 (GRCm39)	missense	probably damaging	1.00
R4771:Zfp608	UTSW	18	55,121,372 (GRCm39)	missense	probably benign
R4820:Zfp608	UTSW	18	55,120,756 (GRCm39)	missense	probably benign	0.14
R4825:Zfp608	UTSW	18	55,031,041 (GRCm39)	missense	probably benign	0.01
R4912:Zfp608	UTSW	18	55,079,663 (GRCm39)	missense	probably damaging	1.00
R4975:Zfp608	UTSW	18	55,022,962 (GRCm39)	missense	probably damaging	1.00
R5138:Zfp608	UTSW	18	55,024,871 (GRCm39)	missense	probably damaging	1.00
R5192:Zfp608	UTSW	18	55,031,569 (GRCm39)	missense	probably damaging	1.00
R5557:Zfp608	UTSW	18	55,120,942 (GRCm39)	missense	possibly damaging	0.81
R5624:Zfp608	UTSW	18	55,031,344 (GRCm39)	missense	probably damaging	1.00
R5818:Zfp608	UTSW	18	55,028,468 (GRCm39)	missense	probably benign	0.02
R5840:Zfp608	UTSW	18	55,031,978 (GRCm39)	missense	probably damaging	1.00
R5992:Zfp608	UTSW	18	55,032,320 (GRCm39)	missense	probably benign	0.15
R6106:Zfp608	UTSW	18	55,120,944 (GRCm39)	missense	possibly damaging	0.54
R6174:Zfp608	UTSW	18	55,121,616 (GRCm39)	start gained	probably benign
R6181:Zfp608	UTSW	18	55,028,700 (GRCm39)	missense	possibly damaging	0.56
R6517:Zfp608	UTSW	18	55,032,150 (GRCm39)	missense	possibly damaging	0.94
R6567:Zfp608	UTSW	18	55,030,628 (GRCm39)	missense	probably damaging	1.00
R6668:Zfp608	UTSW	18	55,031,091 (GRCm39)	missense	probably damaging	1.00
R7061:Zfp608	UTSW	18	55,121,069 (GRCm39)	missense	probably benign
R7074:Zfp608	UTSW	18	55,030,454 (GRCm39)	missense	possibly damaging	0.69
R7087:Zfp608	UTSW	18	55,032,469 (GRCm39)	missense	probably damaging	1.00
R7391:Zfp608	UTSW	18	55,030,619 (GRCm39)	missense	possibly damaging	0.49
R7600:Zfp608	UTSW	18	55,121,092 (GRCm39)	missense	probably damaging	1.00
R7723:Zfp608	UTSW	18	55,030,673 (GRCm39)	missense	probably damaging	1.00
R8054:Zfp608	UTSW	18	55,032,618 (GRCm39)	missense	probably benign	0.28
R8236:Zfp608	UTSW	18	55,032,281 (GRCm39)	missense	probably damaging	1.00
R8260:Zfp608	UTSW	18	55,030,821 (GRCm39)	missense	possibly damaging	0.60
R8732:Zfp608	UTSW	18	55,121,072 (GRCm39)	missense	probably benign	0.06
R8781:Zfp608	UTSW	18	55,031,801 (GRCm39)	missense	probably damaging	1.00
R8851:Zfp608	UTSW	18	55,032,194 (GRCm39)	missense	possibly damaging	0.94
R8940:Zfp608	UTSW	18	55,033,301 (GRCm39)	missense	possibly damaging	0.93
R9051:Zfp608	UTSW	18	55,032,266 (GRCm39)	missense	probably damaging	1.00
R9091:Zfp608	UTSW	18	55,032,190 (GRCm39)	missense	probably damaging	1.00
R9092:Zfp608	UTSW	18	55,031,648 (GRCm39)	missense	probably benign	0.03
R9236:Zfp608	UTSW	18	55,032,354 (GRCm39)	missense	probably benign	0.39
R9270:Zfp608	UTSW	18	55,032,190 (GRCm39)	missense	probably damaging	1.00
R9283:Zfp608	UTSW	18	55,030,913 (GRCm39)	missense	possibly damaging	0.50
R9288:Zfp608	UTSW	18	55,033,341 (GRCm39)	missense	probably benign	0.39
R9463:Zfp608	UTSW	18	55,030,274 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCCAGCGCTGAATTCATG -3'
(R):5'- ATGACAGTGGCGATGATTGG -3'

Sequencing Primer
(F):5'- GCTGAATTCATGCCAGTAGC -3'
(R):5'- CAGTGGCGATGATTGGGAAATC -3'

Posted On

2018-11-06