Mutagenetix > Incidental Mutation

Incidental Mutation 'R6931:Zfp442'

539993

Institutional Source

Beutler Lab

Gene Symbol

Zfp442

Ensembl Gene

ENSMUSG00000068130

Gene Name

zinc finger protein 442

Synonyms

OTTMUSG00000015730

MMRRC Submission

045326-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R6931 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

150249061-150293406 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

C to T at 150252860 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000140098 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109916] [ENSMUST00000185796]

AlphaFold

A2AQA0

Predicted Effect

probably null
Transcript: ENSMUST00000109916

SMART Domains

Protein: ENSMUSP00000105542
Gene: ENSMUSG00000068130

Domain	Start	End	E-Value	Type
KRAB	4	66	3.27e-19	SMART
ZnF_C2H2	159	181	8.34e-3	SMART
ZnF_C2H2	211	233	9.58e-3	SMART
ZnF_C2H2	239	261	2.43e-4	SMART
ZnF_C2H2	267	289	1.38e-3	SMART
ZnF_C2H2	295	317	4.17e-3	SMART
ZnF_C2H2	323	345	3.16e-3	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	9.58e-3	SMART
ZnF_C2H2	407	429	2.09e-3	SMART
ZnF_C2H2	435	457	2.2e-2	SMART
ZnF_C2H2	463	485	1.6e-4	SMART
ZnF_C2H2	491	513	1.82e-3	SMART
ZnF_C2H2	519	541	4.47e-3	SMART
ZnF_C2H2	547	569	3.63e-3	SMART
ZnF_C2H2	575	597	4.79e-3	SMART
ZnF_C2H2	603	625	8.47e-4	SMART
ZnF_C2H2	631	654	3.11e-2	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000185796

SMART Domains

Protein: ENSMUSP00000140098
Gene: ENSMUSG00000068130

Domain	Start	End	E-Value	Type
KRAB	3	65	1.4e-21	SMART
ZnF_C2H2	158	180	3.4e-5	SMART
ZnF_C2H2	210	232	3.9e-5	SMART
ZnF_C2H2	238	260	1e-6	SMART
ZnF_C2H2	266	288	5.6e-6	SMART
ZnF_C2H2	294	316	1.8e-5	SMART
ZnF_C2H2	322	344	1.3e-5	SMART
ZnF_C2H2	350	372	6.7e-6	SMART
ZnF_C2H2	378	400	9.6e-5	SMART
ZnF_C2H2	406	428	6.9e-7	SMART
ZnF_C2H2	434	456	7.7e-6	SMART
ZnF_C2H2	462	484	1.9e-5	SMART
ZnF_C2H2	490	512	1.5e-5	SMART
ZnF_C2H2	518	540	2e-5	SMART
ZnF_C2H2	546	568	3.5e-6	SMART
ZnF_C2H2	574	597	1.3e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

99% (77/78)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310003L06Rik	T	A	5: 88,118,561 (GRCm39)	I15N	probably damaging	Het
2900092C05Rik	T	A	7: 12,246,523 (GRCm39)	S6R	unknown	Het
Abca6	T	A	11: 110,135,154 (GRCm39)	L210F	probably benign	Het
Abcc4	T	C	14: 118,765,400 (GRCm39)	Q919R	probably damaging	Het
Adcy1	A	G	11: 7,100,884 (GRCm39)	D811G	possibly damaging	Het
Akna	A	G	4: 63,305,339 (GRCm39)	S476P	probably benign	Het
Ankrd49	T	C	9: 14,694,122 (GRCm39)	N15S	probably benign	Het
Apcdd1	A	G	18: 63,066,979 (GRCm39)	D31G	probably damaging	Het
Aplp1	G	T	7: 30,142,625 (GRCm39)	R106S	probably damaging	Het
Arhgap40	T	C	2: 158,373,138 (GRCm39)	L132S	probably benign	Het
Atp8b5	G	T	4: 43,364,108 (GRCm39)		probably null	Het
Axl	T	C	7: 25,460,858 (GRCm39)	D717G	probably damaging	Het
Bub1	T	A	2: 127,643,302 (GRCm39)	D1014V	probably damaging	Het
Cacna2d4	T	A	6: 119,259,195 (GRCm39)	V603E	possibly damaging	Het
Cnot11	G	C	1: 39,579,002 (GRCm39)	C289S	probably damaging	Het
Coasy	A	G	11: 100,974,407 (GRCm39)	H191R	probably benign	Het
Cyp11a1	A	T	9: 57,932,403 (GRCm39)	N341Y	possibly damaging	Het
Cyp1a2	T	C	9: 57,589,439 (GRCm39)	N125S	probably benign	Het
Cyp2j8	A	T	4: 96,333,018 (GRCm39)		probably null	Het
Dnah9	A	T	11: 66,008,452 (GRCm39)	I791K	possibly damaging	Het
Ecm2	A	T	13: 49,682,487 (GRCm39)	Q505H	probably benign	Het
Fam135a	T	A	1: 24,124,568 (GRCm39)	M1L	probably damaging	Het
Fam171a2	A	G	11: 102,329,260 (GRCm39)	S500P	possibly damaging	Het
Fat3	A	G	9: 15,871,238 (GRCm39)	S3718P	possibly damaging	Het
Frem1	G	A	4: 82,888,914 (GRCm39)	P1085S	probably damaging	Het
Gcfc2	A	G	6: 81,919,966 (GRCm39)	I390V	probably benign	Het
Gemin4	A	G	11: 76,101,782 (GRCm39)	L993P	probably damaging	Het
Ggnbp2	T	A	11: 84,723,993 (GRCm39)	D647V	probably damaging	Het
Gpr84	A	C	15: 103,217,441 (GRCm39)	L212R	probably damaging	Het
Hnrnpu	T	C	1: 178,158,997 (GRCm39)		probably benign	Het
Hspg2	G	T	4: 137,268,031 (GRCm39)	C2116F	probably damaging	Het
Icam4	T	A	9: 20,941,747 (GRCm39)	V249E	probably damaging	Het
Itga1	T	C	13: 115,138,099 (GRCm39)	N429D	probably benign	Het
Kcns1	T	C	2: 164,006,758 (GRCm39)	T402A	probably damaging	Het
Ky	T	A	9: 102,414,826 (GRCm39)	V246E	probably damaging	Het
Marchf1	A	G	8: 66,921,144 (GRCm39)	T529A	probably benign	Het
Med18	C	G	4: 132,187,194 (GRCm39)	V102L	probably damaging	Het
Mlst8	T	C	17: 24,696,249 (GRCm39)	D160G	probably damaging	Het
Mthfd1	T	C	12: 76,350,472 (GRCm39)	I470T	probably benign	Het
Muc1	C	T	3: 89,136,466 (GRCm39)		probably benign	Het
Mup8	G	A	4: 60,220,322 (GRCm39)	L137F	probably damaging	Het
Mybpc1	A	T	10: 88,378,192 (GRCm39)	L341*	probably null	Het
Nacad	A	G	11: 6,551,877 (GRCm39)	F438S	probably benign	Het
Necap2	C	A	4: 140,805,523 (GRCm39)		probably null	Het
Nifk	T	C	1: 118,260,078 (GRCm39)	L163S	possibly damaging	Het
Npsr1	A	G	9: 24,201,293 (GRCm39)	I73V	probably benign	Het
Nup50l	T	C	6: 96,142,529 (GRCm39)	T172A	possibly damaging	Het
Oog3	C	T	4: 143,885,923 (GRCm39)	C225Y	probably benign	Het
Or2d2	A	G	7: 106,728,108 (GRCm39)	L164P	probably damaging	Het
Or4b13	A	T	2: 90,083,121 (GRCm39)	C70*	probably null	Het
Or4c100	T	A	2: 88,356,538 (GRCm39)	C204S	possibly damaging	Het
Or4p18	G	A	2: 88,232,408 (GRCm39)	T290I	probably benign	Het
Plagl2	C	A	2: 153,077,863 (GRCm39)	K39N	probably benign	Het
Plcg2	T	A	8: 118,284,058 (GRCm39)	D118E	probably benign	Het
Ppp4r3b	A	T	11: 29,161,786 (GRCm39)	K720I	possibly damaging	Het
Prmt3	A	T	7: 49,478,764 (GRCm39)	T442S	probably benign	Het
Prr14l	G	A	5: 32,988,035 (GRCm39)	H487Y	probably damaging	Het
Psmb3	G	A	11: 97,594,797 (GRCm39)	V63I	probably benign	Het
Psmc6	T	A	14: 45,581,182 (GRCm39)	I326K	possibly damaging	Het
Ptcd1	T	C	5: 145,091,885 (GRCm39)	T405A	probably benign	Het
Rbm33	G	A	5: 28,615,743 (GRCm39)	V29M	probably damaging	Het
Rsrc1	C	T	3: 66,901,982 (GRCm39)	P44L	unknown	Het
Scarb1	T	A	5: 125,361,783 (GRCm39)	I107F	probably damaging	Het
Slc39a12	A	G	2: 14,394,186 (GRCm39)	S19G	probably benign	Het
Slc44a5	T	C	3: 153,964,143 (GRCm39)	V503A	probably benign	Het
Slc9a9	T	C	9: 94,552,139 (GRCm39)	S9P	possibly damaging	Het
Snrnp35	A	C	5: 124,628,764 (GRCm39)	R192S	possibly damaging	Het
Tbx15	T	C	3: 99,259,467 (GRCm39)	L446P	probably damaging	Het
Tlnrd1	A	G	7: 83,531,805 (GRCm39)	F209L	probably benign	Het
Tmprss12	C	T	15: 100,183,149 (GRCm39)	R164C	probably damaging	Het
Tnfsf4	T	A	1: 161,244,644 (GRCm39)	F111Y	possibly damaging	Het
Trib2	A	T	12: 15,843,640 (GRCm39)	M198K	probably benign	Het
Ttll8	A	G	15: 88,798,507 (GRCm39)	S743P	possibly damaging	Het
Ush2a	A	T	1: 188,460,580 (GRCm39)	N2614Y	probably benign	Het
Usp6nl	G	A	2: 6,435,269 (GRCm39)	V343I	possibly damaging	Het
Vrtn	C	A	12: 84,697,016 (GRCm39)	Q589K	probably benign	Het
Zbed5	T	A	5: 129,932,170 (GRCm39)	Y706*	probably null	Het
Zc3h10	A	G	10: 128,380,553 (GRCm39)	V268A	probably damaging	Het
Zpld2	A	T	4: 133,920,464 (GRCm39)	M567K	probably benign	Het

Other mutations in Zfp442

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01081:Zfp442	APN	2	150,251,267 (GRCm39)	nonsense	probably null
IGL02566:Zfp442	APN	2	150,251,711 (GRCm39)	critical splice acceptor site	probably null
IGL03217:Zfp442	APN	2	150,251,714 (GRCm39)	splice site	probably benign
LCD18:Zfp442	UTSW	2	150,261,768 (GRCm39)	intron	probably benign
PIT4812001:Zfp442	UTSW	2	150,251,661 (GRCm39)	nonsense	probably null
R0219:Zfp442	UTSW	2	150,253,160 (GRCm39)	missense	probably damaging	0.99
R0521:Zfp442	UTSW	2	150,253,169 (GRCm39)	missense	possibly damaging	0.92
R1633:Zfp442	UTSW	2	150,250,260 (GRCm39)	nonsense	probably null
R1702:Zfp442	UTSW	2	150,251,100 (GRCm39)	nonsense	probably null
R1829:Zfp442	UTSW	2	150,250,983 (GRCm39)	missense	probably damaging	0.99
R1868:Zfp442	UTSW	2	150,250,100 (GRCm39)	missense	probably damaging	1.00
R1898:Zfp442	UTSW	2	150,250,582 (GRCm39)	missense	probably damaging	1.00
R2030:Zfp442	UTSW	2	150,250,042 (GRCm39)	missense	possibly damaging	0.58
R4676:Zfp442	UTSW	2	150,251,526 (GRCm39)	missense	probably damaging	1.00
R4717:Zfp442	UTSW	2	150,250,149 (GRCm39)	missense	probably damaging	1.00
R4894:Zfp442	UTSW	2	150,253,130 (GRCm39)	critical splice donor site	probably null
R4932:Zfp442	UTSW	2	150,251,635 (GRCm39)	missense	possibly damaging	0.53
R4963:Zfp442	UTSW	2	150,250,415 (GRCm39)	missense	probably damaging	1.00
R5130:Zfp442	UTSW	2	150,251,530 (GRCm39)	missense	possibly damaging	0.91
R5476:Zfp442	UTSW	2	150,250,079 (GRCm39)	missense	probably damaging	1.00
R5986:Zfp442	UTSW	2	150,249,944 (GRCm39)	nonsense	probably null
R6042:Zfp442	UTSW	2	150,250,016 (GRCm39)	missense	probably damaging	0.97
R6383:Zfp442	UTSW	2	150,293,321 (GRCm39)	critical splice donor site	probably null
R6452:Zfp442	UTSW	2	150,250,028 (GRCm39)	missense	probably damaging	1.00
R6787:Zfp442	UTSW	2	150,251,499 (GRCm39)	missense	possibly damaging	0.72
R7061:Zfp442	UTSW	2	150,249,937 (GRCm39)	missense	probably benign	0.33
R7184:Zfp442	UTSW	2	150,250,056 (GRCm39)	missense	possibly damaging	0.71
R7214:Zfp442	UTSW	2	150,251,201 (GRCm39)	missense	probably benign	0.04
R7225:Zfp442	UTSW	2	150,250,925 (GRCm39)	missense	probably benign	0.00
R7513:Zfp442	UTSW	2	150,250,676 (GRCm39)	missense	unknown
R7591:Zfp442	UTSW	2	150,250,092 (GRCm39)	nonsense	probably null
R7679:Zfp442	UTSW	2	150,252,917 (GRCm39)	nonsense	probably null
R7768:Zfp442	UTSW	2	150,250,241 (GRCm39)	missense	possibly damaging	0.53
R7801:Zfp442	UTSW	2	150,251,639 (GRCm39)	missense	probably benign	0.28
R7814:Zfp442	UTSW	2	150,251,402 (GRCm39)	missense	possibly damaging	0.92
R7848:Zfp442	UTSW	2	150,253,146 (GRCm39)	missense	possibly damaging	0.71
R8158:Zfp442	UTSW	2	150,251,096 (GRCm39)	missense	possibly damaging	0.83
R8192:Zfp442	UTSW	2	150,250,629 (GRCm39)	missense	unknown
R8528:Zfp442	UTSW	2	150,250,962 (GRCm39)	missense	probably damaging	1.00
R9110:Zfp442	UTSW	2	150,250,093 (GRCm39)	missense	probably benign	0.30
R9269:Zfp442	UTSW	2	150,251,287 (GRCm39)	missense	probably benign	0.19
R9371:Zfp442	UTSW	2	150,250,676 (GRCm39)	missense	unknown
R9401:Zfp442	UTSW	2	150,251,615 (GRCm39)	missense	possibly damaging	0.53
R9459:Zfp442	UTSW	2	150,250,668 (GRCm39)	missense	unknown
R9711:Zfp442	UTSW	2	150,250,207 (GRCm39)	missense	possibly damaging	0.93
Z1177:Zfp442	UTSW	2	150,250,399 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TTTCTTTGTCTTTGCACGTAGAG -3'
(R):5'- GTTGTGACCTATGATGATGTGCA -3'

Sequencing Primer
(F):5'- CTTTGCACGTAGAGGAATGC -3'
(R):5'- CTGGAGACCTACAGGAATCTTGC -3'

Posted On

2018-11-06