Incidental Mutation 'R6938:Secisbp2l'
ID 540367
Institutional Source Beutler Lab
Gene Symbol Secisbp2l
Ensembl Gene ENSMUSG00000035093
Gene Name SECIS binding protein 2-like
Synonyms 3110001I20Rik
MMRRC Submission 045052-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.769) question?
Stock # R6938 (G1)
Quality Score 225.009
Status Not validated
Chromosome 2
Chromosomal Location 125578906-125624790 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to T at 125592272 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Proline to Threonine at position 650 (P650T)
Ref Sequence ENSEMBL: ENSMUSP00000055772 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000053699]
AlphaFold no structure available at present
Predicted Effect probably damaging
Transcript: ENSMUST00000053699
AA Change: P650T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000055772
Gene: ENSMUSG00000035093
AA Change: P650T

DomainStartEndE-ValueType
low complexity region 441 459 N/A INTRINSIC
low complexity region 555 568 N/A INTRINSIC
Pfam:Ribosomal_L7Ae 700 802 7.6e-24 PFAM
low complexity region 821 831 N/A INTRINSIC
low complexity region 970 978 N/A INTRINSIC
low complexity region 985 996 N/A INTRINSIC
Predicted Effect
SMART Domains Protein: ENSMUSP00000121529
Gene: ENSMUSG00000035093
AA Change: P275T

DomainStartEndE-ValueType
low complexity region 67 85 N/A INTRINSIC
low complexity region 181 194 N/A INTRINSIC
Pfam:Ribosomal_L7Ae 326 427 3.5e-24 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.2%
  • 20x: 97.3%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acot7 T C 4: 152,302,351 (GRCm39) probably null Het
Acp6 T C 3: 97,082,949 (GRCm39) M320T probably benign Het
Adam22 A T 5: 8,196,499 (GRCm39) Y259N probably benign Het
Akap9 C A 5: 4,096,628 (GRCm39) A2501D possibly damaging Het
Angpt2 G T 8: 18,748,105 (GRCm39) S385* probably null Het
Asxl2 G C 12: 3,526,149 (GRCm39) R255T probably damaging Het
Btg3 T C 16: 78,157,216 (GRCm39) I244V probably benign Het
Casr T C 16: 36,316,283 (GRCm39) T596A probably damaging Het
Ccl1 A T 11: 82,067,684 (GRCm39) V82D probably damaging Het
Col18a1 T A 10: 76,948,333 (GRCm39) probably benign Het
Crybg1 T C 10: 43,873,379 (GRCm39) D1243G probably benign Het
Dnaaf9 A C 2: 130,617,673 (GRCm39) H297Q probably benign Het
Drc3 A T 11: 60,284,949 (GRCm39) probably null Het
Enpep T C 3: 129,092,599 (GRCm39) D528G probably damaging Het
Fan1 T A 7: 64,022,234 (GRCm39) N340Y probably damaging Het
Gbp4 T C 5: 105,282,943 (GRCm39) D109G probably damaging Het
Gstz1 A G 12: 87,193,943 (GRCm39) probably null Het
Kctd10 C A 5: 114,508,191 (GRCm39) E106* probably null Het
Kctd20 G A 17: 29,180,555 (GRCm39) G110S probably benign Het
Klk1b26 A T 7: 43,665,718 (GRCm39) T177S probably benign Het
Kyat3 T A 3: 142,431,183 (GRCm39) V118E probably damaging Het
Maml3 T C 3: 52,011,159 (GRCm39) K136E probably damaging Het
Mex3d T C 10: 80,218,074 (GRCm39) N381S possibly damaging Het
Mmrn2 A T 14: 34,120,671 (GRCm39) T514S probably benign Het
Nlrp1b G A 11: 71,109,042 (GRCm39) A153V probably damaging Het
Or13e8 C T 4: 43,696,286 (GRCm39) V296M probably damaging Het
Pcolce T C 5: 137,603,878 (GRCm39) T365A probably benign Het
Plbd1 T C 6: 136,593,985 (GRCm39) I377V probably benign Het
Plk2 T A 13: 110,533,214 (GRCm39) Y192* probably null Het
Rev3l T A 10: 39,738,706 (GRCm39) V2820D probably damaging Het
Slc9a5 T C 8: 106,080,064 (GRCm39) L69P probably damaging Het
Sp9 T C 2: 73,103,616 (GRCm39) C57R probably damaging Het
Tbl3 A T 17: 24,924,187 (GRCm39) V190D possibly damaging Het
Timm8b A G 9: 50,516,294 (GRCm39) D49G possibly damaging Het
Tmem231 T C 8: 112,660,144 (GRCm39) S53G probably damaging Het
Topbp1 T A 9: 103,205,753 (GRCm39) V797D probably damaging Het
Trip11 A T 12: 101,803,886 (GRCm39) D1665E probably damaging Het
Txk C A 5: 72,856,492 (GRCm39) R433L probably damaging Het
Vmn1r19 T C 6: 57,381,992 (GRCm39) S182P possibly damaging Het
Vps13b T G 15: 35,423,344 (GRCm39) I221M probably damaging Het
Xpo7 T C 14: 70,903,464 (GRCm39) N1082D probably benign Het
Other mutations in Secisbp2l
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00421:Secisbp2l APN 2 125,585,776 (GRCm39) missense probably damaging 0.99
IGL00644:Secisbp2l APN 2 125,585,764 (GRCm39) missense probably damaging 1.00
IGL01093:Secisbp2l APN 2 125,582,245 (GRCm39) missense probably benign
IGL01621:Secisbp2l APN 2 125,615,131 (GRCm39) missense probably benign
IGL01955:Secisbp2l APN 2 125,585,732 (GRCm39) critical splice donor site probably null
IGL02036:Secisbp2l APN 2 125,600,127 (GRCm39) missense probably benign
IGL02045:Secisbp2l APN 2 125,617,498 (GRCm39) missense possibly damaging 0.82
IGL02182:Secisbp2l APN 2 125,589,497 (GRCm39) missense probably damaging 1.00
IGL02408:Secisbp2l APN 2 125,582,789 (GRCm39) nonsense probably null
IGL02455:Secisbp2l APN 2 125,615,398 (GRCm39) missense possibly damaging 0.89
IGL02953:Secisbp2l APN 2 125,602,194 (GRCm39) missense probably benign 0.36
Rift UTSW 2 125,610,113 (GRCm39) missense probably damaging 1.00
Seismic UTSW 2 125,587,829 (GRCm39) missense probably damaging 1.00
R0097:Secisbp2l UTSW 2 125,613,376 (GRCm39) missense probably damaging 0.96
R0097:Secisbp2l UTSW 2 125,613,376 (GRCm39) missense probably damaging 0.96
R1415:Secisbp2l UTSW 2 125,582,285 (GRCm39) missense probably benign 0.00
R1626:Secisbp2l UTSW 2 125,617,606 (GRCm39) missense probably damaging 0.99
R1926:Secisbp2l UTSW 2 125,582,597 (GRCm39) missense probably damaging 0.99
R1940:Secisbp2l UTSW 2 125,582,259 (GRCm39) missense probably damaging 1.00
R1970:Secisbp2l UTSW 2 125,589,430 (GRCm39) missense probably damaging 1.00
R2100:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2240:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2252:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2253:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2472:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2474:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2475:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2990:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R2993:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3113:Secisbp2l UTSW 2 125,592,206 (GRCm39) missense probably damaging 1.00
R3696:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3749:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3750:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3800:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3810:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3812:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3815:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3816:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3817:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R3880:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4077:Secisbp2l UTSW 2 125,593,785 (GRCm39) splice site probably benign
R4096:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4097:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4164:Secisbp2l UTSW 2 125,593,803 (GRCm39) intron probably benign
R4332:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4418:Secisbp2l UTSW 2 125,594,835 (GRCm39) missense probably benign 0.00
R4598:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4600:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4602:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4603:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4678:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4679:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4684:Secisbp2l UTSW 2 125,587,862 (GRCm39) missense probably damaging 1.00
R4741:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4749:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R4934:Secisbp2l UTSW 2 125,582,409 (GRCm39) missense probably damaging 0.99
R5245:Secisbp2l UTSW 2 125,589,511 (GRCm39) missense probably damaging 1.00
R5521:Secisbp2l UTSW 2 125,594,897 (GRCm39) missense possibly damaging 0.94
R5547:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R5630:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R5631:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R5632:Secisbp2l UTSW 2 125,582,657 (GRCm39) missense possibly damaging 0.66
R6039:Secisbp2l UTSW 2 125,615,136 (GRCm39) missense probably benign 0.28
R6039:Secisbp2l UTSW 2 125,615,136 (GRCm39) missense probably benign 0.28
R6378:Secisbp2l UTSW 2 125,610,245 (GRCm39) missense possibly damaging 0.78
R6616:Secisbp2l UTSW 2 125,610,146 (GRCm39) missense probably damaging 0.96
R7287:Secisbp2l UTSW 2 125,582,289 (GRCm39) missense probably benign
R7373:Secisbp2l UTSW 2 125,599,191 (GRCm39) missense probably damaging 0.99
R7403:Secisbp2l UTSW 2 125,602,199 (GRCm39) missense possibly damaging 0.73
R7484:Secisbp2l UTSW 2 125,613,452 (GRCm39) nonsense probably null
R7504:Secisbp2l UTSW 2 125,600,091 (GRCm39) missense probably benign 0.30
R7762:Secisbp2l UTSW 2 125,610,113 (GRCm39) missense probably damaging 1.00
R7769:Secisbp2l UTSW 2 125,613,465 (GRCm39) critical splice acceptor site probably benign
R8018:Secisbp2l UTSW 2 125,587,829 (GRCm39) missense probably damaging 1.00
R8487:Secisbp2l UTSW 2 125,617,502 (GRCm39) nonsense probably null
R8784:Secisbp2l UTSW 2 125,602,263 (GRCm39) nonsense probably null
R8810:Secisbp2l UTSW 2 125,617,596 (GRCm39) missense possibly damaging 0.82
R8872:Secisbp2l UTSW 2 125,594,892 (GRCm39) missense probably benign
R9111:Secisbp2l UTSW 2 125,602,206 (GRCm39) missense probably benign
R9154:Secisbp2l UTSW 2 125,617,623 (GRCm39) missense probably damaging 1.00
R9155:Secisbp2l UTSW 2 125,617,623 (GRCm39) missense probably damaging 1.00
R9589:Secisbp2l UTSW 2 125,589,430 (GRCm39) missense probably damaging 1.00
R9589:Secisbp2l UTSW 2 125,589,425 (GRCm39) missense probably benign 0.03
R9592:Secisbp2l UTSW 2 125,582,561 (GRCm39) missense probably damaging 1.00
R9602:Secisbp2l UTSW 2 125,609,356 (GRCm39) missense probably benign 0.19
R9620:Secisbp2l UTSW 2 125,589,394 (GRCm39) missense probably damaging 0.99
Predicted Primers PCR Primer
(F):5'- TGCTTTAAATAGACCCACGCCAG -3'
(R):5'- CTGGAGAATTAGGACACAGCC -3'

Sequencing Primer
(F):5'- CCAGCCATGAAGCAAGCGG -3'
(R):5'- TTAGGACACAGCCTGAAAAATTC -3'
Posted On 2018-11-06