Mutagenetix > Incidental Mutation

Incidental Mutation 'R6939:Or5k17'

540465

Institutional Source

Beutler Lab

Gene Symbol

Or5k17

Ensembl Gene

ENSMUSG00000090951

Gene Name

olfactory receptor family 5 subfamily K member 17

Synonyms

Olfr181, MOR184-4, GA_x54KRFPKG5P-55145984-55145034

MMRRC Submission

045053-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.083) question?

Stock #

R6939 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

58745920-58749007 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 58746648 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 95 (Y95*)

Ref Sequence

ENSEMBL: ENSMUSP00000145877 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000075361] [ENSMUST00000205668] [ENSMUST00000205742] [ENSMUST00000205986] [ENSMUST00000206168]

AlphaFold

Q8VGQ7

Predicted Effect

probably null
Transcript: ENSMUST00000075361
AA Change: Y95*

SMART Domains

Protein: ENSMUSP00000074825
Gene: ENSMUSG00000090951
AA Change: Y95*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	305	5.7e-52	PFAM
Pfam:7TM_GPCR_Srsx	35	308	6.1e-6	PFAM
Pfam:7tm_1	41	308	3.3e-19	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000205668
AA Change: Y95*

Predicted Effect

probably null
Transcript: ENSMUST00000205742
AA Change: Y95*

Predicted Effect

probably null
Transcript: ENSMUST00000205986
AA Change: Y95*

Predicted Effect

probably benign
Transcript: ENSMUST00000206168

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.9%

Validation Efficiency

99% (66/67)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

All alleles(1) : Gene trapped(1)

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A1bg	G	A	15: 60,792,244 (GRCm39)	P128L	probably damaging	Het
Acsm3	T	C	7: 119,377,678 (GRCm39)	V401A	probably damaging	Het
Arhgef11	C	A	3: 87,594,227 (GRCm39)	N93K	probably damaging	Het
C1ra	G	A	6: 124,489,760 (GRCm39)		probably null	Het
Camk2a	A	G	18: 61,091,226 (GRCm39)	E236G	probably damaging	Het
Cav2	C	A	6: 17,281,410 (GRCm39)	D17E	possibly damaging	Het
Ccdc149	T	C	5: 52,533,607 (GRCm39)	S520G	probably benign	Het
Chd4	C	A	6: 125,083,501 (GRCm39)	H674Q	probably damaging	Het
Clrn2	A	T	5: 45,611,096 (GRCm39)		probably benign	Het
Cntnap1	A	C	11: 101,077,337 (GRCm39)	I1000L	probably damaging	Het
Cyp2c67	T	C	19: 39,631,778 (GRCm39)	T140A	possibly damaging	Het
D7Ertd443e	T	C	7: 133,966,208 (GRCm39)		probably null	Het
Dcdc2c	G	T	12: 28,591,496 (GRCm39)	Q131K	probably benign	Het
Dmpk	T	A	7: 18,822,149 (GRCm39)	V369E	probably damaging	Het
Dnah11	A	T	12: 118,070,297 (GRCm39)	W1503R	probably damaging	Het
Dnai7	A	G	6: 145,120,945 (GRCm39)	F625L	possibly damaging	Het
Dok2	T	C	14: 71,013,045 (GRCm39)	V71A	probably benign	Het
Dqx1	A	T	6: 83,036,446 (GRCm39)	Q150L	probably damaging	Het
Extl3	A	G	14: 65,304,189 (GRCm39)	I740T	possibly damaging	Het
Fan1	T	A	7: 64,022,234 (GRCm39)	N340Y	probably damaging	Het
Fat2	C	T	11: 55,143,300 (GRCm39)	W4183*	probably null	Het
Fkrp	C	T	7: 16,545,751 (GRCm39)	R37Q	probably benign	Het
Frem3	A	G	8: 81,341,774 (GRCm39)	K1356E	probably benign	Het
Fus	A	T	7: 127,571,741 (GRCm39)		probably benign	Het
Gabarapl2	C	A	8: 112,669,201 (GRCm39)	S53*	probably null	Het
Gjd3	A	C	11: 102,691,733 (GRCm39)	M90R	probably damaging	Het
Gm30083	C	T	14: 33,725,557 (GRCm39)		probably null	Het
Gm7356	T	A	17: 14,221,387 (GRCm39)	Y214F	probably benign	Het
Hexd	A	T	11: 121,113,164 (GRCm39)	D533V	probably benign	Het
Ift172	G	A	5: 31,414,930 (GRCm39)	A1364V	probably damaging	Het
Ighmbp2	A	T	19: 3,326,907 (GRCm39)	F186Y	probably damaging	Het
Inpp5e	C	A	2: 26,297,774 (GRCm39)		probably null	Het
Iqcb1	A	G	16: 36,660,274 (GRCm39)	T146A	possibly damaging	Het
Kcnmb2	A	G	3: 32,252,465 (GRCm39)	Y222C	probably damaging	Het
Kdm6b	A	G	11: 69,297,588 (GRCm39)	W284R	probably damaging	Het
Khdc4	T	A	3: 88,593,824 (GRCm39)	M71K	probably damaging	Het
Ksr2	T	A	5: 117,903,626 (GRCm39)	*952R	probably null	Het
Ldlrap1	C	A	4: 134,495,285 (GRCm39)		probably benign	Het
Mpdu1	G	A	11: 69,548,881 (GRCm39)		probably benign	Het
Mrgprh	A	T	17: 13,095,822 (GRCm39)	T21S	probably benign	Het
Muc16	T	A	9: 18,549,833 (GRCm39)	T5487S	probably benign	Het
Mydgf	A	G	17: 56,490,737 (GRCm39)		probably null	Het
Napa	T	C	7: 15,849,182 (GRCm39)	C241R	possibly damaging	Het
Or10ab4	C	A	7: 107,654,312 (GRCm39)	A41E	possibly damaging	Het
Or10n7-ps1	T	C	9: 39,598,187 (GRCm39)	T18A	possibly damaging	Het
Pla2g4f	A	T	2: 120,137,782 (GRCm39)	L326Q	probably damaging	Het
Pramel16	T	C	4: 143,675,366 (GRCm39)	T487A	probably benign	Het
Psme4	T	A	11: 30,787,291 (GRCm39)	Y1029N	probably damaging	Het
Ptprj	T	C	2: 90,289,858 (GRCm39)	T707A	possibly damaging	Het
Raet1e	T	A	10: 22,050,256 (GRCm39)	M13K	possibly damaging	Het
Rftn2	T	C	1: 55,233,508 (GRCm39)		probably null	Het
Rmi1	A	G	13: 58,557,169 (GRCm39)	I473V	probably benign	Het
Rps6ka4	A	G	19: 6,815,437 (GRCm39)	F186L	probably damaging	Het
Ryr1	T	A	7: 28,751,751 (GRCm39)	M3672L	possibly damaging	Het
Smarca5	A	T	8: 81,431,949 (GRCm39)	C890S	possibly damaging	Het
Spata31d1e	T	A	13: 59,889,872 (GRCm39)	K231N	possibly damaging	Het
Tdrd12	C	T	7: 35,185,024 (GRCm39)		probably null	Het
Tecta	T	C	9: 42,259,293 (GRCm39)	N1530S	probably damaging	Het
Tenm4	G	A	7: 96,202,703 (GRCm39)	R106H	probably benign	Het
Tmem94	A	G	11: 115,676,656 (GRCm39)	S54G	possibly damaging	Het
Tnk2	T	C	16: 32,482,696 (GRCm39)	V41A	probably damaging	Het
Trpm1	A	G	7: 63,918,045 (GRCm39)	T462A	probably benign	Het
Tsg101	T	C	7: 46,556,847 (GRCm39)	T61A	probably benign	Het
Usp42	T	C	5: 143,713,724 (GRCm39)	N17D	probably damaging	Het
Zfp607a	T	A	7: 27,578,473 (GRCm39)	C514*	probably null	Het
Zfp866	G	T	8: 70,218,871 (GRCm39)	Q250K	probably damaging	Het

Other mutations in Or5k17

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01845:Or5k17	APN	16	58,746,929 (GRCm39)	missense	probably benign
IGL02477:Or5k17	APN	16	58,746,126 (GRCm39)	missense	probably benign	0.07
IGL02545:Or5k17	APN	16	58,746,833 (GRCm39)	missense	possibly damaging	0.88
IGL02690:Or5k17	APN	16	58,746,214 (GRCm39)	missense	possibly damaging	0.78
IGL02718:Or5k17	APN	16	58,746,459 (GRCm39)	missense	possibly damaging	0.57
IGL02945:Or5k17	APN	16	58,746,703 (GRCm39)	missense	probably damaging	1.00
IGL03349:Or5k17	APN	16	58,746,323 (GRCm39)	missense	probably benign	0.00
B5639:Or5k17	UTSW	16	58,746,889 (GRCm39)	missense	probably benign	0.00
R0550:Or5k17	UTSW	16	58,746,748 (GRCm39)	missense	probably damaging	1.00
R0659:Or5k17	UTSW	16	58,746,772 (GRCm39)	missense	possibly damaging	0.94
R1433:Or5k17	UTSW	16	58,746,049 (GRCm39)	missense	probably benign
R1957:Or5k17	UTSW	16	58,746,530 (GRCm39)	missense	probably benign
R2155:Or5k17	UTSW	16	58,746,486 (GRCm39)	missense	probably benign	0.01
R2404:Or5k17	UTSW	16	58,745,998 (GRCm39)	missense	probably benign	0.01
R2568:Or5k17	UTSW	16	58,746,286 (GRCm39)	missense	probably benign	0.27
R4022:Or5k17	UTSW	16	58,746,483 (GRCm39)	missense	possibly damaging	0.94
R4592:Or5k17	UTSW	16	58,746,455 (GRCm39)	missense	probably benign	0.00
R4673:Or5k17	UTSW	16	58,746,053 (GRCm39)	missense	possibly damaging	0.61
R4880:Or5k17	UTSW	16	58,746,463 (GRCm39)	missense	probably damaging	0.98
R5109:Or5k17	UTSW	16	58,746,422 (GRCm39)	missense	probably benign	0.10
R5231:Or5k17	UTSW	16	58,746,077 (GRCm39)	missense	possibly damaging	0.94
R5291:Or5k17	UTSW	16	58,746,764 (GRCm39)	missense	possibly damaging	0.96
R5477:Or5k17	UTSW	16	58,746,393 (GRCm39)	missense	possibly damaging	0.61
R5524:Or5k17	UTSW	16	58,746,172 (GRCm39)	missense	probably benign	0.00
R5809:Or5k17	UTSW	16	58,746,860 (GRCm39)	missense	probably benign
R5830:Or5k17	UTSW	16	58,746,457 (GRCm39)	missense	possibly damaging	0.64
R6119:Or5k17	UTSW	16	58,746,895 (GRCm39)	missense	possibly damaging	0.94
R6217:Or5k17	UTSW	16	58,746,877 (GRCm39)	missense	probably benign	0.03
R6861:Or5k17	UTSW	16	58,746,867 (GRCm39)	missense	probably benign
R7376:Or5k17	UTSW	16	58,746,121 (GRCm39)	missense	possibly damaging	0.82
R7650:Or5k17	UTSW	16	58,746,416 (GRCm39)	nonsense	probably null
R8153:Or5k17	UTSW	16	58,746,149 (GRCm39)	missense	possibly damaging	0.47
R8947:Or5k17	UTSW	16	58,746,433 (GRCm39)	missense	probably benign
R9205:Or5k17	UTSW	16	58,746,486 (GRCm39)	missense	probably benign	0.01
R9205:Or5k17	UTSW	16	58,746,485 (GRCm39)	missense	probably benign	0.00
R9318:Or5k17	UTSW	16	58,746,271 (GRCm39)	missense	probably damaging	1.00
R9654:Or5k17	UTSW	16	58,746,752 (GRCm39)	missense	probably benign	0.00
R9678:Or5k17	UTSW	16	58,746,640 (GRCm39)	missense	probably benign	0.17

Predicted Primers

PCR Primer
(F):5'- TGCAGGTTACTGGCTATGAATG -3'
(R):5'- ATCTGAAGACACTTCTGTTCCTGC -3'

Sequencing Primer
(F):5'- GTGCCTATACTCATCTGAATGGAGAG -3'
(R):5'- CATCTATCTGGTCACCATAGTGGG -3'

Posted On

2018-11-06