Mutagenetix > Incidental Mutation

Incidental Mutation 'R6944:Vmn2r14'

540765

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.447) question?

Stock #

R6944 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109215502-109224622 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109216059 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 664 (T664A)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably benign
Transcript: ENSMUST00000170341
AA Change: T664A

PolyPhen 2 Score 0.220 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: T664A

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.2%

Validation Efficiency

97% (75/77)

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932415D10Rik	A	C	10: 82,296,222	I318R	probably benign	Het
Abcb1b	T	G	5: 8,813,693	V216G	probably damaging	Het
Abcb5	T	C	12: 118,911,530	R636G	probably benign	Het
Ago1	G	T	4: 126,460,422	F198L	possibly damaging	Het
Ankrd42	C	T	7: 92,619,547		probably null	Het
Anxa11	T	A	14: 25,874,752	F274I	probably damaging	Het
Ascc1	T	C	10: 60,013,653	L122P	probably damaging	Het
Bptf	T	C	11: 107,080,823	S953G	probably damaging	Het
Chml	T	A	1: 175,688,161	N65Y	probably damaging	Het
Clcc1	A	G	3: 108,670,968	K262E	probably damaging	Het
Clhc1	T	G	11: 29,569,346	D384E	probably damaging	Het
Cnot2	G	A	10: 116,537,223		probably benign	Het
Cntnap1	T	C	11: 101,182,904	V627A	probably damaging	Het
Col6a4	A	G	9: 106,072,171	V755A	probably damaging	Het
Cyp4f18	T	A	8: 71,989,894	I406F	probably benign	Het
Dclre1a	T	C	19: 56,545,019	E381G	possibly damaging	Het
Dnah1	A	G	14: 31,268,904	Y3153H	probably damaging	Het
Dnah8	C	A	17: 30,794,659	D3791E	probably benign	Het
Dnah9	T	C	11: 66,085,149	E1358G	possibly damaging	Het
Eef1g	G	A	19: 8,968,292	R30H	probably benign	Het
Egln3	A	T	12: 54,183,952	I181N	probably benign	Het
Entpd6	A	G	2: 150,763,599	T250A	probably damaging	Het
Epb41l5	C	T	1: 119,609,129	R344Q	probably damaging	Het
Fam20b	T	C	1: 156,687,521	D258G	probably benign	Het
Fbxw18	A	T	9: 109,702,587	D21E	probably damaging	Het
Fbxw21	T	C	9: 109,157,535	E92G	probably damaging	Het
Fzd6	T	A	15: 39,025,817	M110K	possibly damaging	Het
Gm4070	G	A	7: 105,901,980	Q622*	probably null	Het
Gm9268	T	C	7: 43,047,969	F817L	possibly damaging	Het
Golgb1	C	T	16: 36,912,113	P574L	probably benign	Het
Gpr153	A	G	4: 152,279,363	E80G	probably damaging	Het
Kcnt1	T	C	2: 25,877,828		probably benign	Het
Kcnt2	T	G	1: 140,584,065	V962G	probably benign	Het
Malt1	T	C	18: 65,437,920	V109A	probably benign	Het
March7	A	G	2: 60,234,243	I288V	probably benign	Het
Mief1	C	T	15: 80,249,443	R234C	probably damaging	Het
Morc3	A	G	16: 93,870,572	S613G	probably benign	Het
Myt1	A	G	2: 181,797,594	E345G	possibly damaging	Het
Napb	T	C	2: 148,706,969	T133A	probably benign	Het
Nwd1	T	C	8: 72,653,534	V16A	possibly damaging	Het
Oas1f	G	A	5: 120,848,184	E67K	probably benign	Het
Obscn	T	C	11: 59,038,930	K5153R	probably damaging	Het
Olfr1441	G	T	19: 12,423,264	M318I	probably benign	Het
Olfr154	C	T	2: 85,663,851	M194I	probably benign	Het
Olfr212	T	C	6: 116,515,830	F18L	possibly damaging	Het
Olfr317	T	A	11: 58,732,242	S308C	possibly damaging	Het
Pdcd2	T	C	17: 15,525,370	N185S	possibly damaging	Het
Pgap1	A	T	1: 54,530,161	W349R	probably damaging	Het
Prpf39	T	A	12: 65,042,680	V64E	probably benign	Het
Ptpn13	T	A	5: 103,476,991	W54R	probably null	Het
Rbms3	G	T	9: 117,110,105	P29Q	probably damaging	Het
Rnf123	A	G	9: 108,063,623	L679P	probably benign	Het
Samd4	T	A	14: 47,016,635	D84E	possibly damaging	Het
Scarf1	T	C	11: 75,522,206	V426A	probably benign	Het
Slc12a1	A	G	2: 125,160,534	N145D	probably damaging	Het
Slc2a6	A	T	2: 27,026,064	M99K	probably damaging	Het
Slc34a2	A	T	5: 53,064,883	I306L	probably benign	Het
Slx4ip	A	G	2: 137,068,275	K397E	probably damaging	Het
Smr3a	C	T	5: 88,008,090		probably benign	Het
Spef2	T	C	15: 9,592,749	E1502G	probably damaging	Het
Sri	A	T	5: 8,063,365	T119S	probably benign	Het
Synrg	T	A	11: 84,025,086	L1085H	probably damaging	Het
Taf7	A	T	18: 37,642,857	L219H	probably damaging	Het
Tmco3	T	A	8: 13,303,729	V347E	probably damaging	Het
Tmem8b	A	T	4: 43,674,465	I250F	probably damaging	Het
Tom1l2	A	G	11: 60,248,991	V239A	probably damaging	Het
Trpm1	T	A	7: 64,243,433	M1011K	probably damaging	Het
Tspan9	A	T	6: 127,965,806	Y153N	probably benign	Het
Ttll11	T	C	2: 35,752,294	H675R	probably benign	Het
Unc50	C	T	1: 37,432,662	T131M	probably damaging	Het
Vgf	T	A	5: 137,032,352	I456N	probably damaging	Het
Vmn1r223	A	G	13: 23,249,313	I26V	unknown	Het
Vmn1r39	T	C	6: 66,805,221	M1V	probably null	Het
Vmn2r111	T	C	17: 22,559,051	N549S	possibly damaging	Het
Vmn2r96	T	A	17: 18,597,629	F489L	probably benign	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109216314	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109221419	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109224577	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109220409	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109224483	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109220067	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109220588	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109220016	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109221439	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109220188	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109221426	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109216394	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109216107	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109220484	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109219836	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109219044	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109218896	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109216360	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109224574	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109216251	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109220329	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109220329	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109215996	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109221417	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109219972	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109219047	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109221243	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109218832	splice site	probably null
R2417:Vmn2r14	UTSW	5	109224463	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109215910	missense	probably damaging	1.00
R3120:Vmn2r14	UTSW	5	109224565	missense	probably null	0.00
R3729:Vmn2r14	UTSW	5	109216229	missense	probably damaging	1.00
R3762:Vmn2r14	UTSW	5	109220167	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109216064	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109216064	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109216283	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109216283	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109216411	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109216411	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109221504	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109216110	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109221518	splice site	probably null
R4896:Vmn2r14	UTSW	5	109220380	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109220380	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109216095	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109217576	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109221288	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109220395	missense	probably benign	0.44
R5701:Vmn2r14	UTSW	5	109219950	missense	probably damaging	1.00
R5726:Vmn2r14	UTSW	5	109217620	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109215858	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109221356	missense	probably benign
R5985:Vmn2r14	UTSW	5	109220216	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109221417	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109221267	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109216230	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109216274	missense	probably benign	0.06
R7608:Vmn2r14	UTSW	5	109221410	missense	probably benign	0.03
R7740:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109220220	missense	probably benign	0.01
R7804:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R7872:Vmn2r14	UTSW	5	109221353	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109215996	missense	probably benign	0.00
R8006:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R8007:Vmn2r14	UTSW	5	109220458	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109220554	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109221476	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109221474	missense	probably benign	0.30
R8968:Vmn2r14	UTSW	5	109217667	missense	probably benign	0.01
R9008:Vmn2r14	UTSW	5	109220027	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109220188	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109220036	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109219917	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109216221	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109221246	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109221422	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109220310	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109220562	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109220096	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109216175	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109221260	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109219875	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- GCAATGACTGAGCCTTTGTTG -3'
(R):5'- GCCCTCACAGTTCTAGTACTAGTC -3'

Sequencing Primer
(F):5'- TGTATCTCTGTCAATAAAGGGAGG -3'
(R):5'- CACAGTTCTAGTACTAGTCACTTTTG -3'

Posted On

2018-11-28