Mutagenetix > Incidental Mutation

Incidental Mutation 'R6945:Or2g25'

540862

Institutional Source

Beutler Lab

Gene Symbol

Or2g25

Ensembl Gene

ENSMUSG00000095286

Gene Name

olfactory receptor family 2 subfamily G member 25

Synonyms

MOR256-33, GA_x6K02T2PSCP-2119438-2118485, Olfr117

MMRRC Submission

045059-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R6945 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

37970269-37971222 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 37970405 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 273 (I273T)

Ref Sequence

ENSEMBL: ENSMUSP00000150204 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073636] [ENSMUST00000213638] [ENSMUST00000215414]

AlphaFold

L7N1Z1

Predicted Effect

possibly damaging
Transcript: ENSMUST00000073636
AA Change: I273T

PolyPhen 2 Score 0.952 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000073320
Gene: ENSMUSG00000095286
AA Change: I273T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	307	2.7e-44	PFAM
Pfam:7TM_GPCR_Srsx	34	222	2e-5	PFAM
Pfam:7tm_1	40	289	2.9e-23	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213638
AA Change: I273T

PolyPhen 2 Score 0.952 (Sensitivity: 0.79; Specificity: 0.95)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215414
AA Change: I273T

PolyPhen 2 Score 0.952 (Sensitivity: 0.79; Specificity: 0.95)

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.5%

Validation Efficiency

96% (51/53)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc5	T	A	16: 20,218,759 (GRCm39)	T208S	probably benign	Het
Acaa2	A	G	18: 74,926,380 (GRCm39)	E112G	probably benign	Het
Adgrb1	A	G	15: 74,421,873 (GRCm39)	N881S	probably damaging	Het
Adgre1	A	G	17: 57,717,844 (GRCm39)	E314G	probably benign	Het
Adgre1	A	G	17: 57,727,399 (GRCm39)	E443G	probably benign	Het
Akp3	A	G	1: 87,053,353 (GRCm39)	Y102C	probably damaging	Het
Birc6	A	G	17: 74,886,526 (GRCm39)	N618S	probably benign	Het
Bpifc	A	G	10: 85,815,078 (GRCm39)	V296A	probably benign	Het
Cacna2d3	G	A	14: 28,691,275 (GRCm39)		probably benign	Het
Cacnb4	T	C	2: 52,364,966 (GRCm39)	N99S	probably damaging	Het
Cd38	A	G	5: 44,065,348 (GRCm39)	Y283C	probably damaging	Het
Celf4	T	A	18: 25,629,293 (GRCm39)	Q411L	probably damaging	Het
Cemip	A	T	7: 83,647,755 (GRCm39)	H108Q	probably damaging	Het
Col23a1	A	G	11: 51,452,720 (GRCm39)	E225G	unknown	Het
Dnah11	T	C	12: 118,024,045 (GRCm39)	E1902G	probably damaging	Het
Dst	A	G	1: 34,229,571 (GRCm39)	D2063G	probably damaging	Het
Fsip2	A	T	2: 82,823,184 (GRCm39)	I6306L	probably benign	Het
Furin	A	G	7: 80,040,838 (GRCm39)	S667P	possibly damaging	Het
Glmp	T	A	3: 88,233,139 (GRCm39)	S92R	probably benign	Het
Gm11563	C	G	11: 99,549,298 (GRCm39)	C152S	unknown	Het
Gm3486	A	G	14: 41,206,518 (GRCm39)	V185A	probably benign	Het
Gvin2	G	A	7: 105,551,187 (GRCm39)	Q622*	probably null	Het
Hyal1	C	A	9: 107,456,369 (GRCm39)	A102E	probably damaging	Het
Invs	T	C	4: 48,421,785 (GRCm39)	C806R	probably benign	Het
L1td1	T	C	4: 98,621,933 (GRCm39)	V165A	probably benign	Het
Lama1	A	G	17: 68,120,861 (GRCm39)	T2666A		Het
Lrit1	G	C	14: 36,782,052 (GRCm39)	V242L	probably damaging	Het
Lrrc8a	A	G	2: 30,146,239 (GRCm39)	Y351C	probably damaging	Het
Myh7b	T	C	2: 155,464,152 (GRCm39)	F551S	possibly damaging	Het
Myo19	A	G	11: 84,788,386 (GRCm39)	T333A	probably benign	Het
Nrp2	A	T	1: 62,799,947 (GRCm39)	N387I	probably damaging	Het
Oas2	T	C	5: 120,874,204 (GRCm39)	D543G	probably benign	Het
Or5al6	A	G	2: 85,976,428 (GRCm39)	S217P	probably damaging	Het
Pak5	A	G	2: 135,942,859 (GRCm39)	V427A	probably benign	Het
Pfas	G	A	11: 68,891,356 (GRCm39)	A247V	probably benign	Het
Pramel52-ps	C	T	5: 94,531,490 (GRCm39)	H125Y	possibly damaging	Het
Psat1	T	A	19: 15,894,545 (GRCm39)	T115S	probably benign	Het
Psme4	T	A	11: 30,787,437 (GRCm39)	D1077E	probably benign	Het
Rabgap1l	A	G	1: 160,509,752 (GRCm39)	S442P	probably benign	Het
Ralgapa1	A	G	12: 55,822,976 (GRCm39)	M280T	possibly damaging	Het
Rb1cc1	A	T	1: 6,331,256 (GRCm39)	E394D	probably damaging	Het
Seh1l	T	C	18: 67,922,460 (GRCm39)	V271A	probably benign	Het
Sf3b1	A	T	1: 55,036,315 (GRCm39)	N919K	probably benign	Het
Sftpd	A	G	14: 40,896,449 (GRCm39)	S245P	possibly damaging	Het
Slc22a15	T	C	3: 101,831,430 (GRCm39)	E2G	probably damaging	Het
Spta1	A	G	1: 174,036,891 (GRCm39)	D1134G	possibly damaging	Het
Syna	A	G	5: 134,587,815 (GRCm39)	V378A	probably damaging	Het
Tchp	A	G	5: 114,847,411 (GRCm39)	K77E	possibly damaging	Het
Tescl	T	C	7: 24,032,956 (GRCm39)	N123S	probably benign	Het
Trio	C	T	15: 27,824,176 (GRCm39)	R1443Q	probably damaging	Het
Trrap	A	T	5: 144,727,665 (GRCm39)	Y462F	possibly damaging	Het
Vmn1r78	A	G	7: 11,886,832 (GRCm39)	T148A	probably benign	Het
Vmn2r111	T	C	17: 22,778,032 (GRCm39)	N549S	possibly damaging	Het
Zfp1007	A	C	5: 109,824,711 (GRCm39)	N246K	probably benign	Het

Other mutations in Or2g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01862:Or2g25	APN	17	37,970,368 (GRCm39)	missense	probably damaging	1.00
IGL01949:Or2g25	APN	17	37,970,357 (GRCm39)	missense	probably damaging	1.00
IGL02085:Or2g25	APN	17	37,970,579 (GRCm39)	missense	probably benign	0.11
IGL02481:Or2g25	APN	17	37,970,363 (GRCm39)	missense	probably damaging	1.00
IGL02483:Or2g25	APN	17	37,970,363 (GRCm39)	missense	probably damaging	1.00
IGL03274:Or2g25	APN	17	37,970,646 (GRCm39)	missense	probably benign	0.35
R0234:Or2g25	UTSW	17	37,970,997 (GRCm39)	missense	probably damaging	0.99
R0234:Or2g25	UTSW	17	37,970,997 (GRCm39)	missense	probably damaging	0.99
R1522:Or2g25	UTSW	17	37,970,661 (GRCm39)	missense	probably damaging	1.00
R1712:Or2g25	UTSW	17	37,970,799 (GRCm39)	missense	probably benign	0.42
R1750:Or2g25	UTSW	17	37,970,564 (GRCm39)	missense	probably damaging	1.00
R1865:Or2g25	UTSW	17	37,970,754 (GRCm39)	missense	possibly damaging	0.78
R2371:Or2g25	UTSW	17	37,971,044 (GRCm39)	missense	probably damaging	1.00
R2382:Or2g25	UTSW	17	37,970,822 (GRCm39)	missense	probably benign	0.00
R3798:Or2g25	UTSW	17	37,970,997 (GRCm39)	missense	probably damaging	0.99
R4831:Or2g25	UTSW	17	37,970,969 (GRCm39)	missense	probably benign	0.03
R5087:Or2g25	UTSW	17	37,970,612 (GRCm39)	missense	probably damaging	0.97
R5365:Or2g25	UTSW	17	37,970,586 (GRCm39)	missense	probably damaging	1.00
R5812:Or2g25	UTSW	17	37,970,630 (GRCm39)	missense	probably damaging	1.00
R5822:Or2g25	UTSW	17	37,971,122 (GRCm39)	missense	probably damaging	1.00
R6405:Or2g25	UTSW	17	37,971,014 (GRCm39)	missense	possibly damaging	0.58
R7121:Or2g25	UTSW	17	37,970,699 (GRCm39)	missense	probably damaging	0.98
R7312:Or2g25	UTSW	17	37,970,403 (GRCm39)	missense	possibly damaging	0.78
R7502:Or2g25	UTSW	17	37,971,122 (GRCm39)	missense	probably damaging	1.00
R8116:Or2g25	UTSW	17	37,970,631 (GRCm39)	missense	probably damaging	1.00
R8425:Or2g25	UTSW	17	37,970,975 (GRCm39)	missense	probably damaging	1.00
R8960:Or2g25	UTSW	17	37,970,760 (GRCm39)	missense	probably benign	0.02
R9168:Or2g25	UTSW	17	37,971,047 (GRCm39)	missense	probably damaging	1.00
RF017:Or2g25	UTSW	17	37,970,672 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACACCTTCATTATTGTGAGGACAG -3'
(R):5'- GTGCAGCTCTTCATGGCTTC -3'

Sequencing Primer
(F):5'- GAGAGAACTATGTCTCTACCTCG -3'
(R):5'- ACACTCATCTTGGTATCTTATGGG -3'

Posted On

2018-11-28