Mutagenetix > Incidental Mutation

Incidental Mutation 'R6925:Fat4'

541060

Institutional Source

Beutler Lab

Gene Symbol

Fat4

Ensembl Gene

ENSMUSG00000046743

Gene Name

FAT atypical cadherin 4

Synonyms

6030410K14Rik

MMRRC Submission

045043-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6925 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38886940-39011985 bp(+) (GRCm38)

Type of Mutation

critical splice donor site (1 bp from exon)

DNA Base Change (assembly)

G to T at 38996204 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000061836 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061260]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000061260

SMART Domains

Protein: ENSMUSP00000061836
Gene: ENSMUSG00000046743

Domain	Start	End	E-Value	Type
low complexity region	2	21	N/A	INTRINSIC
CA	60	133	4.09e-7	SMART
CA	157	248	4.51e-18	SMART
CA	272	351	7.66e-30	SMART
CA	380	473	2.55e-17	SMART
CA	497	580	8.27e-26	SMART
CA	605	687	6.46e-28	SMART
CA	711	791	1e-24	SMART
CA	815	891	3.78e-20	SMART
CA	915	994	8.6e-24	SMART
CA	1018	1098	7.09e-25	SMART
CA	1122	1208	6.78e-22	SMART
CA	1232	1313	2.63e-28	SMART
CA	1337	1418	7.25e-31	SMART
CA	1442	1527	4.58e-19	SMART
CA	1550	1629	4.52e-9	SMART
CA	1651	1738	1.3e-9	SMART
CA	1762	1839	2.01e-24	SMART
CA	1863	1942	3.11e-21	SMART
CA	1966	2049	5.85e-26	SMART
CA	2072	2152	1.88e-29	SMART
CA	2176	2257	3.06e-29	SMART
CA	2282	2362	2.61e-23	SMART
CA	2386	2466	2.99e-32	SMART
CA	2490	2568	9.92e-6	SMART
CA	2588	2669	6.58e-20	SMART
CA	2692	2773	7.25e-31	SMART
CA	2796	2872	1.69e-22	SMART
CA	2896	2983	3.16e-22	SMART
CA	3007	3089	1.01e-15	SMART
CA	3113	3194	1.25e-25	SMART
CA	3218	3298	7e-15	SMART
CA	3322	3405	3.96e-14	SMART
CA	3428	3510	3.41e-27	SMART
CA	3532	3614	5.64e-19	SMART
EGF	3807	3862	1.78e-2	SMART
EGF_CA	3864	3900	2.36e-16	SMART
EGF_CA	3902	3938	7.99e-14	SMART
EGF	3943	3976	1.24e-1	SMART
LamG	3996	4144	4.08e-19	SMART
EGF	4167	4200	5.88e-3	SMART
LamG	4244	4375	1.76e-23	SMART
EGF	4430	4464	1.41e-5	SMART
low complexity region	4514	4526	N/A	INTRINSIC
low complexity region	4533	4550	N/A	INTRINSIC
low complexity region	4840	4849	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 98.0%

Validation Efficiency

100% (91/91)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the protocadherin family. This gene may play a role in regulating planar cell polarity (PCP). Studies in mice suggest that loss of PCP signaling may cause cystic kidney disease, and mutations in this gene have been associated with Van Maldergem Syndrome 2. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Mar 2014]
PHENOTYPE: Homozygous inactivation of this gene leads to neonatal lethality, reduced birth body size, curly tails, kyphosis, small lungs, renal cysts, and defects in sternum and vertebrae morphology, neural tube width, cochlear elongation, stereocilia orientation, kidney development, and intestinal elongation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 90 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5430419D17Rik	A	G	7: 131,222,707 (GRCm38)	Q177R	possibly damaging	Het
Acat1	A	T	9: 53,592,029 (GRCm38)	V170E	probably benign	Het
Atp2b2	T	A	6: 113,760,720 (GRCm38)	I902F	probably damaging	Het
Cacna1d	A	G	14: 30,051,637 (GRCm38)	V1697A	probably benign	Het
Ccdc14	T	A	16: 34,690,749 (GRCm38)	F31Y	probably benign	Het
Ccdc17	T	A	4: 116,598,210 (GRCm38)	V250E	probably damaging	Het
Cct7	A	G	6: 85,459,182 (GRCm38)	N25S	probably damaging	Het
Cep19	G	C	16: 32,103,942 (GRCm38)	G9R	probably damaging	Het
Ces5a	A	T	8: 93,523,057 (GRCm38)		probably null	Het
Chd1l	T	G	3: 97,582,826 (GRCm38)	E471A	probably damaging	Het
Chd6	A	G	2: 161,013,127 (GRCm38)	I787T	probably damaging	Het
Cntnap5c	A	C	17: 58,395,266 (GRCm38)	T1194P	probably benign	Het
Col6a3	T	A	1: 90,816,002 (GRCm38)	M208L	probably benign	Het
Coro7	A	T	16: 4,628,674 (GRCm38)		probably null	Het
Csrnp2	T	C	15: 100,481,958 (GRCm38)	K484R	probably benign	Het
Cyp2c39	T	A	19: 39,513,195 (GRCm38)	V64E	probably damaging	Het
Ddr2	T	C	1: 169,998,132 (GRCm38)	K300E	probably benign	Het
Ddx56	T	C	11: 6,263,980 (GRCm38)	E393G	probably damaging	Het
Diaph1	A	C	18: 37,853,679 (GRCm38)	Y1084*	probably null	Het
Disp1	A	T	1: 183,086,478 (GRCm38)	F1459L	probably benign	Het
Dnajc8	G	A	4: 132,544,113 (GRCm38)	A80T	probably damaging	Het
Elfn1	T	A	5: 139,971,685 (GRCm38)	M148K	probably benign	Het
Emcn	T	A	3: 137,419,002 (GRCm38)	I154N	probably damaging	Het
Enah	T	A	1: 181,905,899 (GRCm38)		probably null	Het
Enah	C	G	1: 181,905,898 (GRCm38)		probably null	Het
Ep300	C	A	15: 81,649,981 (GRCm38)	Q2080K	probably benign	Het
Epha2	T	A	4: 141,308,757 (GRCm38)	M168K	probably benign	Het
Ercc6	T	A	14: 32,562,608 (GRCm38)	I776N	probably damaging	Het
Fan1	T	A	7: 64,372,486 (GRCm38)	N340Y	probably damaging	Het
G3bp1	A	G	11: 55,497,960 (GRCm38)	R333G	possibly damaging	Het
Gm3604	A	G	13: 62,369,390 (GRCm38)	F385L	probably benign	Het
Gm5800	T	C	14: 51,713,700 (GRCm38)	I147M	possibly damaging	Het
Hipk1	A	G	3: 103,778,245 (GRCm38)	F18S	unknown	Het
Ighv3-8	A	T	12: 114,322,330 (GRCm38)	C131S	probably benign	Het
Il23r	T	G	6: 67,423,493 (GRCm38)	T618P	probably damaging	Het
Il31ra	G	A	13: 112,527,529 (GRCm38)	T538I	possibly damaging	Het
Kcns2	A	G	15: 34,839,913 (GRCm38)	D474G	unknown	Het
Klc4	T	C	17: 46,636,229 (GRCm38)	T407A	possibly damaging	Het
Klra5	C	T	6: 129,911,457 (GRCm38)	S2N	probably benign	Het
Krt10	T	C	11: 99,388,851 (GRCm38)	Y161C	probably damaging	Het
Kxd1	A	T	8: 70,523,278 (GRCm38)	M1K	probably null	Het
Lgals1	A	C	15: 78,928,040 (GRCm38)	D27A	possibly damaging	Het
Lgr5	T	C	10: 115,466,346 (GRCm38)	S308G	probably benign	Het
Lrp1	G	A	10: 127,556,988 (GRCm38)	S2736L	probably benign	Het
Lrpap1	G	T	5: 35,102,536 (GRCm38)	H73N	probably benign	Het
Mcpt1	C	A	14: 56,019,065 (GRCm38)	T86K	probably damaging	Het
Megf6	A	T	4: 154,254,587 (GRCm38)	D527V	probably damaging	Het
Mical3	A	T	6: 120,959,390 (GRCm38)	S1392T	probably benign	Het
Mmp2	A	T	8: 92,839,382 (GRCm38)	D437V	probably damaging	Het
Mob4	T	A	1: 55,152,722 (GRCm38)	Y166*	probably null	Het
Mrap2	G	A	9: 87,182,474 (GRCm38)	M89I	possibly damaging	Het
Mug1	G	A	6: 121,881,787 (GRCm38)	A1155T	probably damaging	Het
Myh2	G	T	11: 67,193,218 (GRCm38)	A1556S	probably benign	Het
Nfatc3	A	G	8: 106,119,322 (GRCm38)	D1028G	probably benign	Het
Ngef	T	G	1: 87,503,263 (GRCm38)		probably null	Het
Nlrp1a	A	T	11: 71,092,513 (GRCm38)	L1209Q	probably null	Het
Npr2	C	T	4: 43,647,553 (GRCm38)	R819C	probably damaging	Het
Nsd2	A	T	5: 33,879,110 (GRCm38)	D646V	probably damaging	Het
Nsun7	A	G	5: 66,277,072 (GRCm38)	H252R	probably damaging	Het
Olfr267	A	T	4: 58,785,647 (GRCm38)	I25N	possibly damaging	Het
Olfr447	T	A	6: 42,911,857 (GRCm38)	C111*	probably null	Het
Olfr642	C	T	7: 104,049,740 (GRCm38)	V205I	probably benign	Het
Olfr951	G	T	9: 39,393,861 (GRCm38)	Q20H	probably benign	Het
Olfr951	A	T	9: 39,393,860 (GRCm38)	Q20L	probably benign	Het
Pcdhga2	A	T	18: 37,670,585 (GRCm38)	D494V	probably damaging	Het
Pcsk2	A	G	2: 143,813,747 (GRCm38)	E617G	probably damaging	Het
Pdc	T	C	1: 150,333,180 (GRCm38)	I138T	probably damaging	Het
Phc2	T	A	4: 128,748,134 (GRCm38)	S750T	probably damaging	Het
Pkd2l1	T	A	19: 44,191,508 (GRCm38)	N88Y	possibly damaging	Het
Plcl1	C	T	1: 55,406,598 (GRCm38)	R71*	probably null	Het
Prag1	C	A	8: 36,103,894 (GRCm38)	L544M	probably damaging	Het
Prkg2	A	G	5: 98,966,510 (GRCm38)		probably null	Het
Psd2	G	A	18: 35,979,711 (GRCm38)	S153N	probably damaging	Het
Rab11fip1	A	T	8: 27,152,972 (GRCm38)	S600T	probably damaging	Het
Rbms1	T	G	2: 60,762,304 (GRCm38)	T222P	probably benign	Het
Slfn8	A	T	11: 83,013,417 (GRCm38)	Y382*	probably null	Het
Socs4	T	A	14: 47,289,738 (GRCm38)	C43*	probably null	Het
Sorl1	T	A	9: 42,033,626 (GRCm38)	T868S	probably damaging	Het
St6gal1	A	G	16: 23,356,213 (GRCm38)	Y267C	probably damaging	Het
Syne1	A	G	10: 5,126,682 (GRCm38)	V6879A	probably benign	Het
Syne2	C	A	12: 75,854,132 (GRCm38)	H22N	possibly damaging	Het
Tmeff2	T	A	1: 50,928,021 (GRCm38)	L25Q	probably damaging	Het
Tmprss11g	G	T	5: 86,487,426 (GRCm38)	D396E	probably benign	Het
Tmprss11g	T	A	5: 86,487,436 (GRCm38)	H393L	probably benign	Het
Vmn2r23	A	G	6: 123,704,553 (GRCm38)	E140G	probably damaging	Het
Wapl	T	C	14: 34,677,363 (GRCm38)	S130P	probably benign	Het
Zeb2	T	A	2: 44,994,529 (GRCm38)	K982M	probably damaging	Het
Zfhx3	C	G	8: 108,956,821 (GRCm38)	H3631D	unknown	Het
Zfp719	T	C	7: 43,590,706 (GRCm38)	S573P	probably damaging	Het
Zfp979	A	T	4: 147,613,542 (GRCm38)	C237S	possibly damaging	Het

Other mutations in Fat4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Fat4	APN	3	38,982,249 (GRCm38)	missense	probably damaging	1.00
IGL00509:Fat4	APN	3	38,889,039 (GRCm38)	missense	probably damaging	1.00
IGL00698:Fat4	APN	3	38,981,145 (GRCm38)	missense	probably benign	0.17
IGL00934:Fat4	APN	3	38,890,673 (GRCm38)	missense	probably damaging	1.00
IGL01063:Fat4	APN	3	38,890,579 (GRCm38)	missense	possibly damaging	0.80
IGL01123:Fat4	APN	3	38,957,269 (GRCm38)	missense	probably benign	0.00
IGL01313:Fat4	APN	3	39,007,201 (GRCm38)	missense	possibly damaging	0.53
IGL01328:Fat4	APN	3	38,980,658 (GRCm38)	missense	probably damaging	1.00
IGL01328:Fat4	APN	3	38,889,991 (GRCm38)	missense	probably damaging	1.00
IGL01374:Fat4	APN	3	38,887,498 (GRCm38)	missense	probably damaging	1.00
IGL01412:Fat4	APN	3	38,891,181 (GRCm38)	missense	probably benign	0.09
IGL01472:Fat4	APN	3	38,888,070 (GRCm38)	missense	probably damaging	1.00
IGL01514:Fat4	APN	3	38,949,534 (GRCm38)	missense	possibly damaging	0.89
IGL01548:Fat4	APN	3	39,009,257 (GRCm38)	missense	probably damaging	1.00
IGL01548:Fat4	APN	3	38,887,758 (GRCm38)	missense	probably damaging	0.99
IGL01576:Fat4	APN	3	38,888,947 (GRCm38)	missense	probably damaging	1.00
IGL01591:Fat4	APN	3	39,010,375 (GRCm38)	nonsense	probably null
IGL01626:Fat4	APN	3	38,951,032 (GRCm38)	missense	probably damaging	1.00
IGL01746:Fat4	APN	3	38,991,731 (GRCm38)	nonsense	probably null
IGL01800:Fat4	APN	3	38,981,729 (GRCm38)	missense	probably damaging	0.99
IGL01815:Fat4	APN	3	38,888,773 (GRCm38)	missense	probably damaging	1.00
IGL01863:Fat4	APN	3	38,970,619 (GRCm38)	splice site	probably benign
IGL01917:Fat4	APN	3	38,889,730 (GRCm38)	missense	possibly damaging	0.89
IGL01936:Fat4	APN	3	38,979,774 (GRCm38)	missense	probably benign	0.10
IGL02060:Fat4	APN	3	39,010,271 (GRCm38)	missense	probably damaging	1.00
IGL02103:Fat4	APN	3	38,889,199 (GRCm38)	missense	probably damaging	0.97
IGL02119:Fat4	APN	3	38,982,939 (GRCm38)	missense	probably benign	0.10
IGL02124:Fat4	APN	3	38,888,404 (GRCm38)	missense	probably damaging	1.00
IGL02164:Fat4	APN	3	38,996,205 (GRCm38)	critical splice donor site	probably null
IGL02182:Fat4	APN	3	38,890,546 (GRCm38)	missense	probably damaging	1.00
IGL02207:Fat4	APN	3	38,951,263 (GRCm38)	missense	probably benign	0.16
IGL02210:Fat4	APN	3	38,891,853 (GRCm38)	missense	probably benign	0.01
IGL02257:Fat4	APN	3	39,001,139 (GRCm38)	missense	probably benign	0.09
IGL02271:Fat4	APN	3	38,979,919 (GRCm38)	missense	probably benign	0.18
IGL02305:Fat4	APN	3	39,009,988 (GRCm38)	missense	probably damaging	1.00
IGL02314:Fat4	APN	3	38,887,630 (GRCm38)	missense	probably damaging	1.00
IGL02455:Fat4	APN	3	38,951,131 (GRCm38)	missense	possibly damaging	0.48
IGL02468:Fat4	APN	3	38,983,046 (GRCm38)	missense	probably benign
IGL02478:Fat4	APN	3	38,888,215 (GRCm38)	missense	probably damaging	1.00
IGL02480:Fat4	APN	3	39,010,430 (GRCm38)	missense	probably damaging	1.00
IGL02487:Fat4	APN	3	38,887,245 (GRCm38)	missense	probably damaging	1.00
IGL02632:Fat4	APN	3	39,002,764 (GRCm38)	missense	probably benign	0.04
IGL02665:Fat4	APN	3	39,002,836 (GRCm38)	missense	probably benign	0.08
IGL02674:Fat4	APN	3	38,983,337 (GRCm38)	missense	probably benign	0.35
IGL02692:Fat4	APN	3	38,951,086 (GRCm38)	missense	probably damaging	1.00
IGL02710:Fat4	APN	3	38,890,595 (GRCm38)	missense	probably damaging	1.00
IGL02803:Fat4	APN	3	38,889,295 (GRCm38)	missense	probably damaging	1.00
IGL02834:Fat4	APN	3	38,956,744 (GRCm38)	missense	probably damaging	1.00
IGL02891:Fat4	APN	3	38,951,273 (GRCm38)	missense	probably damaging	1.00
IGL02982:Fat4	APN	3	38,890,843 (GRCm38)	missense	probably damaging	1.00
IGL02993:Fat4	APN	3	38,957,155 (GRCm38)	missense	probably damaging	1.00
IGL02996:Fat4	APN	3	38,958,525 (GRCm38)	missense	probably damaging	1.00
IGL03029:Fat4	APN	3	38,982,591 (GRCm38)	missense	possibly damaging	0.46
IGL03124:Fat4	APN	3	38,981,552 (GRCm38)	missense	possibly damaging	0.61
IGL03144:Fat4	APN	3	38,956,859 (GRCm38)	missense	possibly damaging	0.68
IGL03149:Fat4	APN	3	38,991,685 (GRCm38)	missense	probably damaging	1.00
IGL03169:Fat4	APN	3	38,957,398 (GRCm38)	missense	probably benign	0.02
IGL03190:Fat4	APN	3	38,981,241 (GRCm38)	missense	probably damaging	1.00
IGL03272:Fat4	APN	3	39,009,703 (GRCm38)	missense	probably benign
IGL03371:Fat4	APN	3	38,983,187 (GRCm38)	missense	possibly damaging	0.65
IGL03372:Fat4	APN	3	38,889,134 (GRCm38)	missense	possibly damaging	0.88
IGL03388:Fat4	APN	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
IGL03394:Fat4	APN	3	38,892,019 (GRCm38)	missense	probably damaging	0.99
IGL03394:Fat4	APN	3	39,009,364 (GRCm38)	missense	probably damaging	1.00
IGL03405:Fat4	APN	3	38,958,450 (GRCm38)	missense	probably benign	0.02
IGL03410:Fat4	APN	3	38,891,176 (GRCm38)	missense	probably damaging	1.00
Asahi	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
Expulsion	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
heineken	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
schlitz	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
PIT4696001:Fat4	UTSW	3	38,982,357 (GRCm38)	missense	probably damaging	0.98
PIT4696001:Fat4	UTSW	3	38,889,004 (GRCm38)	missense	probably benign	0.04
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0015:Fat4	UTSW	3	38,982,503 (GRCm38)	missense	probably damaging	1.00
R0078:Fat4	UTSW	3	38,888,931 (GRCm38)	missense	probably benign	0.35
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0100:Fat4	UTSW	3	38,980,248 (GRCm38)	missense	probably damaging	1.00
R0201:Fat4	UTSW	3	38,891,596 (GRCm38)	missense	probably damaging	0.99
R0280:Fat4	UTSW	3	38,890,816 (GRCm38)	missense	probably benign
R0357:Fat4	UTSW	3	38,891,227 (GRCm38)	missense	probably damaging	1.00
R0409:Fat4	UTSW	3	38,977,413 (GRCm38)	missense	probably damaging	1.00
R0498:Fat4	UTSW	3	38,980,637 (GRCm38)	missense	probably benign	0.00
R0502:Fat4	UTSW	3	39,002,924 (GRCm38)	missense	probably damaging	0.98
R0506:Fat4	UTSW	3	38,888,314 (GRCm38)	missense	probably benign	0.00
R0532:Fat4	UTSW	3	38,981,721 (GRCm38)	missense	probably benign	0.02
R0616:Fat4	UTSW	3	38,942,870 (GRCm38)	missense	probably damaging	1.00
R0630:Fat4	UTSW	3	39,000,172 (GRCm38)	missense	probably damaging	1.00
R0678:Fat4	UTSW	3	38,889,694 (GRCm38)	missense	probably damaging	1.00
R0685:Fat4	UTSW	3	39,001,178 (GRCm38)	missense	probably benign
R0729:Fat4	UTSW	3	39,000,295 (GRCm38)	splice site	probably benign
R0748:Fat4	UTSW	3	38,887,828 (GRCm38)	missense	possibly damaging	0.67
R0811:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0812:Fat4	UTSW	3	38,957,474 (GRCm38)	missense	probably damaging	1.00
R0830:Fat4	UTSW	3	38,999,109 (GRCm38)	missense	probably benign	0.26
R0841:Fat4	UTSW	3	38,995,998 (GRCm38)	missense	probably damaging	0.99
R0884:Fat4	UTSW	3	38,982,858 (GRCm38)	missense	possibly damaging	0.89
R1056:Fat4	UTSW	3	38,891,392 (GRCm38)	missense	probably damaging	1.00
R1066:Fat4	UTSW	3	38,957,227 (GRCm38)	missense	probably damaging	1.00
R1078:Fat4	UTSW	3	38,983,086 (GRCm38)	missense	probably benign	0.10
R1084:Fat4	UTSW	3	38,979,825 (GRCm38)	missense	possibly damaging	0.88
R1118:Fat4	UTSW	3	38,982,942 (GRCm38)	missense	possibly damaging	0.88
R1213:Fat4	UTSW	3	38,890,371 (GRCm38)	missense	probably benign	0.01
R1418:Fat4	UTSW	3	38,890,813 (GRCm38)	missense	probably damaging	1.00
R1475:Fat4	UTSW	3	38,888,323 (GRCm38)	missense	probably damaging	1.00
R1487:Fat4	UTSW	3	38,995,917 (GRCm38)	missense	possibly damaging	0.77
R1511:Fat4	UTSW	3	38,983,076 (GRCm38)	missense	probably damaging	0.97
R1534:Fat4	UTSW	3	38,890,089 (GRCm38)	missense	probably damaging	1.00
R1558:Fat4	UTSW	3	38,888,986 (GRCm38)	missense	probably damaging	1.00
R1586:Fat4	UTSW	3	38,888,860 (GRCm38)	missense	probably damaging	1.00
R1592:Fat4	UTSW	3	39,007,177 (GRCm38)	missense	probably damaging	0.99
R1655:Fat4	UTSW	3	38,957,318 (GRCm38)	missense	probably damaging	0.97
R1662:Fat4	UTSW	3	38,980,779 (GRCm38)	missense	probably damaging	1.00
R1710:Fat4	UTSW	3	38,951,155 (GRCm38)	missense	probably damaging	1.00
R1731:Fat4	UTSW	3	38,891,310 (GRCm38)	missense	probably damaging	1.00
R1761:Fat4	UTSW	3	38,887,489 (GRCm38)	missense	possibly damaging	0.61
R1770:Fat4	UTSW	3	39,010,268 (GRCm38)	missense	probably damaging	1.00
R1828:Fat4	UTSW	3	38,983,458 (GRCm38)	missense	probably damaging	1.00
R1835:Fat4	UTSW	3	38,983,571 (GRCm38)	missense	probably benign	0.00
R1846:Fat4	UTSW	3	38,982,383 (GRCm38)	missense	probably benign	0.00
R1861:Fat4	UTSW	3	39,010,484 (GRCm38)	missense	probably benign	0.09
R1871:Fat4	UTSW	3	38,981,072 (GRCm38)	missense	possibly damaging	0.63
R1981:Fat4	UTSW	3	38,991,664 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,996,090 (GRCm38)	missense	probably damaging	1.00
R1988:Fat4	UTSW	3	38,887,115 (GRCm38)	missense	probably benign
R2056:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2058:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2059:Fat4	UTSW	3	38,891,170 (GRCm38)	missense	possibly damaging	0.88
R2070:Fat4	UTSW	3	39,010,655 (GRCm38)	missense	probably benign	0.00
R2078:Fat4	UTSW	3	38,889,673 (GRCm38)	missense	probably damaging	1.00
R2114:Fat4	UTSW	3	38,981,484 (GRCm38)	missense	probably benign	0.01
R2135:Fat4	UTSW	3	38,980,733 (GRCm38)	missense	probably damaging	0.98
R2152:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2153:Fat4	UTSW	3	38,983,395 (GRCm38)	missense	probably damaging	1.00
R2154:Fat4	UTSW	3	38,887,539 (GRCm38)	missense	probably damaging	1.00
R2196:Fat4	UTSW	3	38,981,417 (GRCm38)	missense	probably benign	0.23
R2211:Fat4	UTSW	3	38,891,527 (GRCm38)	missense	possibly damaging	0.77
R2219:Fat4	UTSW	3	39,010,215 (GRCm38)	missense	probably damaging	1.00
R2247:Fat4	UTSW	3	38,892,049 (GRCm38)	missense	probably damaging	1.00
R2263:Fat4	UTSW	3	38,888,989 (GRCm38)	missense	possibly damaging	0.93
R2264:Fat4	UTSW	3	38,890,422 (GRCm38)	missense	probably benign	0.25
R2274:Fat4	UTSW	3	38,995,899 (GRCm38)	missense	possibly damaging	0.47
R2337:Fat4	UTSW	3	38,980,011 (GRCm38)	missense	probably damaging	1.00
R2343:Fat4	UTSW	3	38,957,105 (GRCm38)	missense	probably damaging	0.97
R2365:Fat4	UTSW	3	38,980,419 (GRCm38)	missense	probably benign
R2412:Fat4	UTSW	3	38,957,072 (GRCm38)	missense	probably benign	0.05
R2883:Fat4	UTSW	3	38,980,804 (GRCm38)	missense	probably damaging	1.00
R2942:Fat4	UTSW	3	38,982,336 (GRCm38)	missense	probably damaging	1.00
R2989:Fat4	UTSW	3	39,007,153 (GRCm38)	missense	probably benign
R3103:Fat4	UTSW	3	38,891,940 (GRCm38)	missense	probably benign	0.03
R3158:Fat4	UTSW	3	38,890,791 (GRCm38)	missense	possibly damaging	0.87
R3800:Fat4	UTSW	3	38,981,274 (GRCm38)	missense	possibly damaging	0.48
R3808:Fat4	UTSW	3	38,982,438 (GRCm38)	missense	possibly damaging	0.52
R3848:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3850:Fat4	UTSW	3	39,007,261 (GRCm38)	missense	probably benign	0.10
R3957:Fat4	UTSW	3	38,982,346 (GRCm38)	missense	probably benign
R4065:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign	0.13
R4078:Fat4	UTSW	3	38,980,020 (GRCm38)	missense	probably damaging	1.00
R4096:Fat4	UTSW	3	38,887,875 (GRCm38)	missense	possibly damaging	0.46
R4161:Fat4	UTSW	3	38,942,809 (GRCm38)	missense	possibly damaging	0.95
R4273:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R4285:Fat4	UTSW	3	38,889,171 (GRCm38)	missense	probably benign	0.00
R4288:Fat4	UTSW	3	38,891,763 (GRCm38)	missense	probably damaging	1.00
R4407:Fat4	UTSW	3	38,958,540 (GRCm38)	missense	probably benign	0.05
R4528:Fat4	UTSW	3	38,891,294 (GRCm38)	missense	probably benign	0.01
R4547:Fat4	UTSW	3	38,951,283 (GRCm38)	missense	probably damaging	1.00
R4681:Fat4	UTSW	3	38,887,342 (GRCm38)	missense	probably damaging	1.00
R4826:Fat4	UTSW	3	38,982,957 (GRCm38)	missense	probably damaging	1.00
R4855:Fat4	UTSW	3	38,888,317 (GRCm38)	missense	probably benign
R4871:Fat4	UTSW	3	38,891,605 (GRCm38)	missense	probably damaging	1.00
R4897:Fat4	UTSW	3	38,980,632 (GRCm38)	missense	probably damaging	1.00
R4928:Fat4	UTSW	3	39,010,465 (GRCm38)	missense	probably damaging	1.00
R4932:Fat4	UTSW	3	39,007,203 (GRCm38)	missense	probably benign	0.00
R4941:Fat4	UTSW	3	38,957,452 (GRCm38)	missense	probably damaging	1.00
R4943:Fat4	UTSW	3	38,980,173 (GRCm38)	missense	probably benign	0.19
R4959:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R4973:Fat4	UTSW	3	38,983,046 (GRCm38)	missense	probably benign	0.00
R5098:Fat4	UTSW	3	38,888,289 (GRCm38)	missense	probably benign	0.34
R5163:Fat4	UTSW	3	38,980,797 (GRCm38)	missense	probably damaging	1.00
R5213:Fat4	UTSW	3	38,980,191 (GRCm38)	missense	possibly damaging	0.56
R5328:Fat4	UTSW	3	38,956,868 (GRCm38)	missense	probably damaging	1.00
R5337:Fat4	UTSW	3	39,010,378 (GRCm38)	missense	probably benign	0.44
R5337:Fat4	UTSW	3	38,891,627 (GRCm38)	missense	probably damaging	1.00
R5363:Fat4	UTSW	3	38,888,005 (GRCm38)	missense	probably damaging	1.00
R5380:Fat4	UTSW	3	38,888,864 (GRCm38)	missense	probably damaging	1.00
R5384:Fat4	UTSW	3	38,995,946 (GRCm38)	missense	possibly damaging	0.87
R5422:Fat4	UTSW	3	38,887,245 (GRCm38)	missense	possibly damaging	0.92
R5436:Fat4	UTSW	3	38,891,346 (GRCm38)	missense	probably benign	0.00
R5443:Fat4	UTSW	3	39,010,370 (GRCm38)	missense	probably damaging	1.00
R5501:Fat4	UTSW	3	38,887,215 (GRCm38)	missense	probably benign	0.09
R5571:Fat4	UTSW	3	39,010,274 (GRCm38)	missense	probably damaging	1.00
R5625:Fat4	UTSW	3	38,888,934 (GRCm38)	missense	possibly damaging	0.78
R5652:Fat4	UTSW	3	39,002,968 (GRCm38)	missense	probably damaging	0.99
R5725:Fat4	UTSW	3	38,889,625 (GRCm38)	missense	probably damaging	1.00
R5735:Fat4	UTSW	3	38,949,576 (GRCm38)	missense	probably damaging	1.00
R5739:Fat4	UTSW	3	38,983,134 (GRCm38)	missense	probably benign	0.01
R5766:Fat4	UTSW	3	38,889,468 (GRCm38)	missense	probably damaging	1.00
R5780:Fat4	UTSW	3	38,980,955 (GRCm38)	missense	probably damaging	0.96
R5811:Fat4	UTSW	3	38,891,787 (GRCm38)	missense	probably damaging	1.00
R5829:Fat4	UTSW	3	39,007,305 (GRCm38)	missense	probably damaging	1.00
R5879:Fat4	UTSW	3	38,887,336 (GRCm38)	missense	probably benign
R5933:Fat4	UTSW	3	38,951,375 (GRCm38)	critical splice donor site	probably null
R5938:Fat4	UTSW	3	38,951,239 (GRCm38)	missense	probably damaging	1.00
R5940:Fat4	UTSW	3	38,889,649 (GRCm38)	missense	probably benign	0.00
R5945:Fat4	UTSW	3	38,983,206 (GRCm38)	missense	probably benign	0.19
R5963:Fat4	UTSW	3	39,010,547 (GRCm38)	missense	probably damaging	1.00
R6077:Fat4	UTSW	3	39,002,802 (GRCm38)	missense	probably damaging	1.00
R6158:Fat4	UTSW	3	38,983,262 (GRCm38)	missense	possibly damaging	0.95
R6246:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	1.00
R6253:Fat4	UTSW	3	38,951,356 (GRCm38)	missense	probably damaging	0.99
R6259:Fat4	UTSW	3	39,007,246 (GRCm38)	missense	probably benign	0.18
R6295:Fat4	UTSW	3	39,007,080 (GRCm38)	splice site	probably null
R6387:Fat4	UTSW	3	38,983,785 (GRCm38)	missense	probably damaging	1.00
R6390:Fat4	UTSW	3	38,980,380 (GRCm38)	missense	probably damaging	1.00
R6456:Fat4	UTSW	3	38,983,979 (GRCm38)	missense	possibly damaging	0.90
R6493:Fat4	UTSW	3	38,890,887 (GRCm38)	missense	probably damaging	1.00
R6500:Fat4	UTSW	3	38,981,269 (GRCm38)	nonsense	probably null
R6503:Fat4	UTSW	3	38,982,257 (GRCm38)	missense	probably benign	0.00
R6519:Fat4	UTSW	3	39,002,871 (GRCm38)	missense	probably benign
R6566:Fat4	UTSW	3	38,957,126 (GRCm38)	missense	possibly damaging	0.78
R6576:Fat4	UTSW	3	38,979,690 (GRCm38)	missense	probably benign
R6590:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6658:Fat4	UTSW	3	38,942,928 (GRCm38)	missense	probably benign	0.01
R6662:Fat4	UTSW	3	38,956,821 (GRCm38)	missense	possibly damaging	0.95
R6690:Fat4	UTSW	3	38,983,539 (GRCm38)	missense	probably damaging	1.00
R6807:Fat4	UTSW	3	38,982,440 (GRCm38)	missense	probably benign	0.18
R6823:Fat4	UTSW	3	38,983,939 (GRCm38)	missense	probably benign	0.05
R6824:Fat4	UTSW	3	38,957,525 (GRCm38)	missense	probably benign	0.00
R6830:Fat4	UTSW	3	38,981,817 (GRCm38)	missense	probably benign	0.00
R6948:Fat4	UTSW	3	39,009,446 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,995,971 (GRCm38)	missense	probably damaging	1.00
R6970:Fat4	UTSW	3	38,981,775 (GRCm38)	missense	probably damaging	1.00
R7017:Fat4	UTSW	3	38,891,543 (GRCm38)	missense	probably benign
R7030:Fat4	UTSW	3	38,981,958 (GRCm38)	missense	probably damaging	1.00
R7044:Fat4	UTSW	3	39,010,811 (GRCm38)	missense	probably benign	0.02
R7044:Fat4	UTSW	3	39,010,810 (GRCm38)	missense	probably benign
R7045:Fat4	UTSW	3	38,888,601 (GRCm38)	missense	probably benign	0.01
R7094:Fat4	UTSW	3	38,889,874 (GRCm38)	missense	probably damaging	1.00
R7111:Fat4	UTSW	3	39,010,533 (GRCm38)	missense	probably damaging	1.00
R7130:Fat4	UTSW	3	38,980,787 (GRCm38)	missense	probably damaging	0.99
R7168:Fat4	UTSW	3	38,980,659 (GRCm38)	missense	probably damaging	1.00
R7192:Fat4	UTSW	3	38,980,464 (GRCm38)	missense	probably benign	0.04
R7194:Fat4	UTSW	3	38,983,895 (GRCm38)	missense	probably damaging	1.00
R7194:Fat4	UTSW	3	38,888,884 (GRCm38)	missense	probably damaging	1.00
R7199:Fat4	UTSW	3	38,977,362 (GRCm38)	missense	probably damaging	0.98
R7213:Fat4	UTSW	3	38,999,087 (GRCm38)	missense	possibly damaging	0.63
R7216:Fat4	UTSW	3	38,891,043 (GRCm38)	missense	probably damaging	1.00
R7225:Fat4	UTSW	3	38,980,176 (GRCm38)	missense	possibly damaging	0.50
R7238:Fat4	UTSW	3	38,890,413 (GRCm38)	missense	probably benign	0.31
R7239:Fat4	UTSW	3	38,983,840 (GRCm38)	missense	possibly damaging	0.85
R7283:Fat4	UTSW	3	38,889,693 (GRCm38)	missense	probably damaging	1.00
R7296:Fat4	UTSW	3	38,889,145 (GRCm38)	nonsense	probably null
R7372:Fat4	UTSW	3	38,890,209 (GRCm38)	missense	probably damaging	1.00
R7400:Fat4	UTSW	3	38,887,924 (GRCm38)	missense	probably damaging	1.00
R7419:Fat4	UTSW	3	39,000,236 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	39,009,644 (GRCm38)	missense	probably damaging	1.00
R7430:Fat4	UTSW	3	38,887,450 (GRCm38)	missense	probably damaging	0.97
R7431:Fat4	UTSW	3	39,009,157 (GRCm38)	missense	possibly damaging	0.80
R7486:Fat4	UTSW	3	38,957,427 (GRCm38)	nonsense	probably null
R7501:Fat4	UTSW	3	38,958,448 (GRCm38)	nonsense	probably null
R7533:Fat4	UTSW	3	39,007,257 (GRCm38)	missense	probably benign	0.43
R7542:Fat4	UTSW	3	38,981,621 (GRCm38)	missense	possibly damaging	0.64
R7542:Fat4	UTSW	3	38,981,355 (GRCm38)	missense	possibly damaging	0.56
R7548:Fat4	UTSW	3	38,981,114 (GRCm38)	missense	probably benign	0.13
R7567:Fat4	UTSW	3	38,889,336 (GRCm38)	missense	probably damaging	1.00
R7644:Fat4	UTSW	3	39,010,241 (GRCm38)	missense	possibly damaging	0.64
R7660:Fat4	UTSW	3	38,981,160 (GRCm38)	missense	probably benign
R7665:Fat4	UTSW	3	38,889,178 (GRCm38)	missense	probably benign	0.00
R7676:Fat4	UTSW	3	38,891,697 (GRCm38)	missense	probably damaging	0.98
R7832:Fat4	UTSW	3	39,001,204 (GRCm38)	missense	probably benign	0.00
R7848:Fat4	UTSW	3	38,887,851 (GRCm38)	missense	probably benign
R7883:Fat4	UTSW	3	38,981,819 (GRCm38)	missense	probably damaging	1.00
R7892:Fat4	UTSW	3	38,949,439 (GRCm38)	critical splice acceptor site	probably null
R7904:Fat4	UTSW	3	38,887,541 (GRCm38)	missense	probably damaging	1.00
R7952:Fat4	UTSW	3	38,891,721 (GRCm38)	missense	probably damaging	0.98
R8015:Fat4	UTSW	3	38,981,916 (GRCm38)	missense	possibly damaging	0.79
R8040:Fat4	UTSW	3	38,981,666 (GRCm38)	missense	probably damaging	1.00
R8142:Fat4	UTSW	3	38,891,203 (GRCm38)	missense	probably damaging	1.00
R8151:Fat4	UTSW	3	38,892,054 (GRCm38)	missense	probably damaging	0.99
R8163:Fat4	UTSW	3	38,979,732 (GRCm38)	missense	possibly damaging	0.88
R8317:Fat4	UTSW	3	38,958,510 (GRCm38)	missense	possibly damaging	0.80
R8413:Fat4	UTSW	3	39,008,979 (GRCm38)	critical splice acceptor site	probably null
R8447:Fat4	UTSW	3	38,979,675 (GRCm38)	missense	possibly damaging	0.88
R8458:Fat4	UTSW	3	38,981,553 (GRCm38)	missense	probably benign	0.25
R8509:Fat4	UTSW	3	38,981,903 (GRCm38)	missense	probably benign
R8543:Fat4	UTSW	3	38,977,494 (GRCm38)	missense	probably damaging	1.00
R8679:Fat4	UTSW	3	39,010,693 (GRCm38)	missense	probably damaging	1.00
R8726:Fat4	UTSW	3	39,010,498 (GRCm38)	missense	probably damaging	1.00
R8743:Fat4	UTSW	3	38,888,443 (GRCm38)	missense	probably benign	0.16
R8751:Fat4	UTSW	3	38,891,853 (GRCm38)	missense	probably benign	0.01
R8779:Fat4	UTSW	3	38,979,749 (GRCm38)	missense	probably damaging	1.00
R8797:Fat4	UTSW	3	38,999,129 (GRCm38)	missense	probably benign	0.01
R8860:Fat4	UTSW	3	38,892,120 (GRCm38)	missense	probably benign	0.26
R8955:Fat4	UTSW	3	38,983,629 (GRCm38)	missense	probably benign	0.01
R9053:Fat4	UTSW	3	38,887,175 (GRCm38)	nonsense	probably null
R9071:Fat4	UTSW	3	38,983,449 (GRCm38)	missense	probably benign	0.29
R9088:Fat4	UTSW	3	39,007,299 (GRCm38)	missense	probably benign	0.02
R9100:Fat4	UTSW	3	39,010,654 (GRCm38)	missense
R9180:Fat4	UTSW	3	38,888,407 (GRCm38)	missense	possibly damaging	0.78
R9184:Fat4	UTSW	3	38,982,443 (GRCm38)	missense	probably damaging	0.99
R9201:Fat4	UTSW	3	38,890,930 (GRCm38)	missense	probably damaging	1.00
R9206:Fat4	UTSW	3	39,009,241 (GRCm38)	missense	probably damaging	0.99
R9268:Fat4	UTSW	3	38,888,247 (GRCm38)	missense	probably damaging	1.00
R9278:Fat4	UTSW	3	38,891,022 (GRCm38)	missense	probably benign	0.44
R9287:Fat4	UTSW	3	38,891,632 (GRCm38)	missense	probably damaging	0.98
R9355:Fat4	UTSW	3	38,981,898 (GRCm38)	missense	probably damaging	1.00
R9437:Fat4	UTSW	3	38,891,268 (GRCm38)	missense	probably benign	0.00
R9455:Fat4	UTSW	3	38,891,263 (GRCm38)	missense
R9456:Fat4	UTSW	3	38,888,422 (GRCm38)	missense	possibly damaging	0.50
R9476:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9510:Fat4	UTSW	3	38,983,737 (GRCm38)	missense	probably benign	0.04
R9511:Fat4	UTSW	3	38,980,653 (GRCm38)	missense	probably damaging	0.98
R9540:Fat4	UTSW	3	39,009,197 (GRCm38)	missense	probably benign
R9568:Fat4	UTSW	3	38,892,007 (GRCm38)	missense	probably damaging	1.00
R9646:Fat4	UTSW	3	38,981,664 (GRCm38)	missense	probably damaging	1.00
R9683:Fat4	UTSW	3	38,889,183 (GRCm38)	missense	possibly damaging	0.52
R9711:Fat4	UTSW	3	39,001,225 (GRCm38)	missense	probably benign	0.00
X0017:Fat4	UTSW	3	39,009,106 (GRCm38)	missense	probably benign	0.00
X0019:Fat4	UTSW	3	38,981,040 (GRCm38)	missense	probably damaging	1.00
X0020:Fat4	UTSW	3	39,000,151 (GRCm38)	missense	probably damaging	1.00
X0024:Fat4	UTSW	3	38,942,902 (GRCm38)	missense	probably benign	0.43
X0064:Fat4	UTSW	3	38,970,752 (GRCm38)	missense	probably damaging	1.00
Z1088:Fat4	UTSW	3	38,958,492 (GRCm38)	missense	probably benign	0.00
Z1088:Fat4	UTSW	3	38,887,050 (GRCm38)	missense	possibly damaging	0.88
Z1176:Fat4	UTSW	3	38,983,815 (GRCm38)	missense	probably damaging	1.00
Z1176:Fat4	UTSW	3	38,983,359 (GRCm38)	missense	probably benign	0.00
Z1177:Fat4	UTSW	3	38,890,347 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,888,584 (GRCm38)	missense	probably damaging	1.00
Z1177:Fat4	UTSW	3	38,981,838 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTTTGCCACCATCAAAAGC -3'
(R):5'- GACGGCTGAGAAAGAATCGTTC -3'

Sequencing Primer
(F):5'- TTGCCACCATCAAAAGCCATGC -3'
(R):5'- CGGCTGAGAAAGAATCGTTCAATATG -3'

Posted On

2018-11-28