Mutagenetix > Incidental Mutation

Incidental Mutation 'R6953:Ogfod3'

541325

Institutional Source

Beutler Lab

Gene Symbol

Ogfod3

Ensembl Gene

ENSMUSG00000025169

Gene Name

2-oxoglutarate and iron-dependent oxygenase domain containing 3

Synonyms

1110031I02Rik

MMRRC Submission

045065-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R6953 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

121068419-121095474 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 121093824 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 62 (I62T)

Ref Sequence

ENSEMBL: ENSMUSP00000026169 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000026169] [ENSMUST00000038831] [ENSMUST00000106117] [ENSMUST00000124768]

AlphaFold

Q9D136

Predicted Effect

probably benign
Transcript: ENSMUST00000026169
AA Change: I62T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000026169
Gene: ENSMUSG00000025169
AA Change: I62T

Domain	Start	End	E-Value	Type
transmembrane domain	39	61	N/A	INTRINSIC
P4Hc	104	302	1.82e-25	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000038831

SMART Domains

Protein: ENSMUSP00000048479
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	30	223	2.9e-12	PFAM
low complexity region	528	538	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106117

SMART Domains

Protein: ENSMUSP00000101723
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	31	229	1.7e-18	PFAM
low complexity region	455	465	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124768

SMART Domains

Protein: ENSMUSP00000122008
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	22	152	8.3e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000151495

SMART Domains

Protein: ENSMUSP00000123073
Gene: ENSMUSG00000039307

Domain	Start	End	E-Value	Type
Pfam:Glyco_hydro_20	27	220	3.1e-12	PFAM
low complexity region	525	535	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.2%
20x: 97.3%

Validation Efficiency

98% (54/55)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130401M01Rik	C	A	15: 57,892,223 (GRCm39)	S128I	probably damaging	Het
Aadacl2	A	C	3: 59,932,181 (GRCm39)	H232P	possibly damaging	Het
Abcc3	A	G	11: 94,265,661 (GRCm39)	Y125H	probably benign	Het
Adgrb3	A	G	1: 25,865,592 (GRCm39)	S84P	probably damaging	Het
Adgrd1	A	T	5: 129,192,142 (GRCm39)	K71*	probably null	Het
Ap2m1	T	A	16: 20,361,468 (GRCm39)	W381R	probably damaging	Het
Ascc3	A	G	10: 50,521,762 (GRCm39)	I426V	probably benign	Het
BC024063	T	A	10: 81,943,733 (GRCm39)	D31E	possibly damaging	Het
C3ar1	G	A	6: 122,827,591 (GRCm39)	H209Y	possibly damaging	Het
Ccp110	T	C	7: 118,321,644 (GRCm39)	V433A	possibly damaging	Het
Cfap54	A	G	10: 92,830,540 (GRCm39)	S1199P	probably benign	Het
Cyp24a1	T	G	2: 170,329,866 (GRCm39)	D362A	probably benign	Het
Dapk2	C	G	9: 66,161,904 (GRCm39)	R271G	probably benign	Het
Dnmt1	G	T	9: 20,829,822 (GRCm39)	Q633K	probably benign	Het
Ercc4	T	A	16: 12,948,550 (GRCm39)	V499D	probably damaging	Het
Ier3ip1	C	G	18: 77,027,309 (GRCm39)	P46R	probably damaging	Het
Ifi211	T	C	1: 173,733,832 (GRCm39)	T110A	probably damaging	Het
Isoc1	A	G	18: 58,804,374 (GRCm39)	D134G	possibly damaging	Het
Kif26b	A	G	1: 178,701,637 (GRCm39)	D672G	possibly damaging	Het
Klhl33	T	A	14: 51,128,973 (GRCm39)	D752V	possibly damaging	Het
Lcn4	A	G	2: 26,559,367 (GRCm39)	Y133H	probably benign	Het
Mrs2	C	A	13: 25,185,771 (GRCm39)	V134L	probably benign	Het
Muc16	T	C	9: 18,551,825 (GRCm39)	T4823A	probably benign	Het
Or2m13	C	T	16: 19,226,278 (GRCm39)	V164I	probably benign	Het
Or2y10	A	G	11: 49,455,117 (GRCm39)	Y123C	probably damaging	Het
Or6c204	A	T	10: 129,022,474 (GRCm39)	M272K	probably benign	Het
Or7e175	A	T	9: 20,049,299 (GRCm39)	I296L	probably benign	Het
Papln	G	A	12: 83,828,659 (GRCm39)	W788*	probably null	Het
Pcdhac2	A	G	18: 37,277,479 (GRCm39)	Q153R	probably benign	Het
Pcdhgb2	C	T	18: 37,823,807 (GRCm39)	T266I	possibly damaging	Het
Pcdhgc5	C	A	18: 37,953,514 (GRCm39)	R263S	possibly damaging	Het
Phactr4	T	C	4: 132,104,662 (GRCm39)	T185A	possibly damaging	Het
Plcxd2	T	C	16: 45,800,882 (GRCm39)	D114G	probably damaging	Het
Polr2a	A	T	11: 69,632,537 (GRCm39)	I987N	probably damaging	Het
Prl3d3	A	G	13: 27,345,029 (GRCm39)	M134V	probably benign	Het
Pum2	T	A	12: 8,778,779 (GRCm39)		probably null	Het
Racgap1	T	C	15: 99,524,210 (GRCm39)	E399G	probably damaging	Het
Sbspon	A	T	1: 15,930,519 (GRCm39)	S156T	probably damaging	Het
Scn1a	T	C	2: 66,149,813 (GRCm39)	T927A	probably damaging	Het
Sec23ip	C	A	7: 128,354,520 (GRCm39)	S78*	probably null	Het
Serpina1d	G	A	12: 103,733,989 (GRCm39)	T105I	probably benign	Het
Slc25a21	T	A	12: 57,205,954 (GRCm39)	N26Y	probably benign	Het
Smim20	T	A	5: 53,435,258 (GRCm39)	D64E	probably damaging	Het
Spag17	T	G	3: 99,942,291 (GRCm39)	I772S	possibly damaging	Het
Tars2	T	C	3: 95,660,426 (GRCm39)	T99A	possibly damaging	Het
Tdrd1	C	T	19: 56,819,803 (GRCm39)	T101I	probably damaging	Het
Tldc2	T	G	2: 156,931,198 (GRCm39)		probably null	Het
Ttn	A	G	2: 76,601,929 (GRCm39)	Y10251H	probably damaging	Het
Ush2a	A	G	1: 187,995,342 (GRCm39)	R38G	possibly damaging	Het
Usp19	T	C	9: 108,376,130 (GRCm39)	L991P	possibly damaging	Het
Vmn2r109	G	A	17: 20,760,973 (GRCm39)	P795S	possibly damaging	Het
Vmn2r26	T	C	6: 124,016,741 (GRCm39)	Y402H	probably benign	Het
Vmn2r-ps117	T	G	17: 19,045,095 (GRCm39)	I504R	probably benign	Het
Zfp551	A	T	7: 12,150,715 (GRCm39)	C231*	probably null	Het
Zfp607a	T	A	7: 27,577,790 (GRCm39)	C287S	possibly damaging	Het
Zim1	T	A	7: 6,690,706 (GRCm39)	T40S	unknown	Het

Other mutations in Ogfod3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01954:Ogfod3	APN	11	121,093,851 (GRCm39)	missense	probably benign	0.06
IGL02398:Ogfod3	APN	11	121,093,851 (GRCm39)	missense	probably benign	0.06
IGL02458:Ogfod3	APN	11	121,091,749 (GRCm39)	missense	probably benign	0.22
IGL03411:Ogfod3	APN	11	121,068,630 (GRCm39)	makesense	probably null
PIT4403001:Ogfod3	UTSW	11	121,087,561 (GRCm39)	critical splice donor site	probably null
R0145:Ogfod3	UTSW	11	121,085,896 (GRCm39)	splice site	probably benign
R1302:Ogfod3	UTSW	11	121,074,300 (GRCm39)	missense	probably damaging	1.00
R3926:Ogfod3	UTSW	11	121,074,255 (GRCm39)	missense	probably damaging	1.00
R4823:Ogfod3	UTSW	11	121,086,027 (GRCm39)	missense	probably benign	0.42
R4825:Ogfod3	UTSW	11	121,086,027 (GRCm39)	missense	probably benign	0.42
R4909:Ogfod3	UTSW	11	121,088,318 (GRCm39)	missense	probably damaging	1.00
R6378:Ogfod3	UTSW	11	121,093,761 (GRCm39)	missense	probably benign	0.01
R7051:Ogfod3	UTSW	11	121,086,031 (GRCm39)	missense	probably damaging	0.99
R7618:Ogfod3	UTSW	11	121,093,804 (GRCm39)	missense	probably damaging	0.96
R7741:Ogfod3	UTSW	11	121,074,362 (GRCm39)	critical splice acceptor site	probably null
R8112:Ogfod3	UTSW	11	121,095,376 (GRCm39)	missense	probably damaging	1.00
R8714:Ogfod3	UTSW	11	121,087,608 (GRCm39)	missense	possibly damaging	0.79
R9769:Ogfod3	UTSW	11	121,074,357 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCTGAGTTTCATTTCTGTCACG -3'
(R):5'- TCCAGTGTGAACATCTTGTGAG -3'

Sequencing Primer
(F):5'- TCTCCTGAAGGTGAAGTTACAGGC -3'
(R):5'- AACATCTTGTGAGTTTGATTGGC -3'

Posted On

2018-11-28