Mutagenetix > Incidental Mutation

Incidental Mutation 'R0606:Fryl'

54136

Institutional Source

Beutler Lab

Gene Symbol

Fryl

Ensembl Gene

ENSMUSG00000070733

Gene Name

FRY like transcription coactivator

Synonyms

2510002A14Rik, 2310004H21Rik, 9030227G01Rik

MMRRC Submission

038795-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.758) question?

Stock #

R0606 (G1)

Quality Score

163

Status

Validated

Chromosome

Chromosomal Location

73019987-73256619 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 73124734 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Leucine at position 174 (H174L)

Ref Sequence

ENSEMBL: ENSMUSP00000098687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094700] [ENSMUST00000101127] [ENSMUST00000152631] [ENSMUST00000153903]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000094700
AA Change: H174L

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000092289
Gene: ENSMUSG00000070733
AA Change: H174L

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	117	649	5.7e-176	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	896	1141	1.3e-5	PFAM
Pfam:MOR2-PAG1_mid	1145	1331	2e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1450	1.2e-5	PFAM
low complexity region	1476	1487	N/A	INTRINSIC
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1590	1660	1.1e-5	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	3.2e-15	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2002	2255	9.9e-78	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000101127
AA Change: H174L

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000098687
Gene: ENSMUSG00000070733
AA Change: H174L

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	116	649	3e-172	PFAM
low complexity region	873	884	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	898	1115	7.8e-6	PFAM
Pfam:MOR2-PAG1_mid	1147	1331	9.5e-19	PFAM
Pfam:MOR2-PAG1_mid	1351	1503	1.1e-5	PFAM
low complexity region	1530	1548	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1589	1664	6e-6	PFAM
Pfam:MOR2-PAG1_mid	1725	1866	1.6e-14	PFAM
low complexity region	1973	1984	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	1998	2260	4.4e-76	PFAM
low complexity region	2329	2341	N/A	INTRINSIC
low complexity region	2473	2482	N/A	INTRINSIC
low complexity region	2485	2500	N/A	INTRINSIC
low complexity region	2523	2534	N/A	INTRINSIC
coiled coil region	2625	2649	N/A	INTRINSIC
low complexity region	2827	2841	N/A	INTRINSIC
coiled coil region	2854	2893	N/A	INTRINSIC
coiled coil region	2963	2989	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148433

Predicted Effect

probably benign
Transcript: ENSMUST00000152631

SMART Domains

Protein: ENSMUSP00000115208
Gene: ENSMUSG00000070733

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	1	117	5.3e-40	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000153903

SMART Domains

Protein: ENSMUSP00000119385
Gene: ENSMUSG00000070733

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	1	199	4.4e-58	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000153923

Meta Mutation Damage Score

0.3417

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.3%
20x: 94.2%

Validation Efficiency

98% (99/101)

MGI Phenotype

PHENOTYPE: Most mice homozygous for a knock-out allele exhibit postnatal lethality and defects in kidney development; rare survivors display growth retardation, decreased body weight, and premature death associated with chronic hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 95 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410131K14Rik	T	C	5: 118,259,089 (GRCm38)	Y128H	probably damaging	Het
Actl9	T	C	17: 33,433,598 (GRCm38)	Y211H	probably damaging	Het
Actn1	A	T	12: 80,174,647 (GRCm38)		probably benign	Het
Adtrp	A	G	13: 41,767,405 (GRCm38)	F197L	probably damaging	Het
Ankrd11	G	A	8: 122,892,832 (GRCm38)	T1406I	probably benign	Het
Arhgap24	A	T	5: 102,897,220 (GRCm38)	R620W	probably damaging	Het
Atg13	A	G	2: 91,682,073 (GRCm38)	Y284H	probably benign	Het
Atrn	A	G	2: 130,906,856 (GRCm38)	E99G	possibly damaging	Het
Cage1	A	T	13: 38,016,494 (GRCm38)		probably benign	Het
Ccr3	T	A	9: 124,028,802 (GRCm38)	M58K	probably benign	Het
Cdk18	G	T	1: 132,117,617 (GRCm38)		probably benign	Het
Chst5	A	G	8: 111,890,919 (GRCm38)	V23A	probably benign	Het
Col4a3	T	C	1: 82,672,586 (GRCm38)		probably benign	Het
Col4a6	A	G	X: 141,192,223 (GRCm38)		probably benign	Het
Csmd3	T	C	15: 48,457,662 (GRCm38)	I251V	probably benign	Het
Csnk1g3	G	A	18: 53,917,028 (GRCm38)	V115M	probably damaging	Het
Cst7	A	T	2: 150,570,519 (GRCm38)	M1L	probably benign	Het
Cyp4f17	A	T	17: 32,527,843 (GRCm38)	D373V	probably damaging	Het
Dclk2	G	A	3: 86,906,004 (GRCm38)	R212W	probably damaging	Het
Dhrs7b	T	G	11: 60,830,746 (GRCm38)		probably benign	Het
Dhx58	T	A	11: 100,702,251 (GRCm38)	H210L	probably benign	Het
Dnah9	T	C	11: 65,841,333 (GRCm38)	Y4249C	probably damaging	Het
Eif5b	T	A	1: 38,048,893 (GRCm38)	L990H	probably damaging	Het
Faap24	T	C	7: 35,394,963 (GRCm38)		probably benign	Het
Gabrr1	T	C	4: 33,132,696 (GRCm38)	W15R	probably benign	Het
Gif	A	T	19: 11,752,294 (GRCm38)	I206F	possibly damaging	Het
Gm15446	T	C	5: 109,943,481 (GRCm38)	V533A	probably benign	Het
Gm6760	T	A	X: 64,151,653 (GRCm38)	K63*	probably null	Het
Gne	C	T	4: 44,042,244 (GRCm38)	E444K	possibly damaging	Het
Gpr173	T	A	X: 152,347,040 (GRCm38)	M146L	possibly damaging	Het
Hira	C	T	16: 18,935,047 (GRCm38)	S547L	probably benign	Het
Hnf1b	A	G	11: 83,863,984 (GRCm38)	H161R	probably benign	Het
Hnrnpm	T	A	17: 33,658,390 (GRCm38)	N53I	probably damaging	Het
Hs3st5	A	T	10: 36,832,588 (GRCm38)	I40F	probably benign	Het
Hydin	C	T	8: 110,549,798 (GRCm38)		probably benign	Het
Ift172	A	G	5: 31,254,313 (GRCm38)	I1607T	probably damaging	Het
Igfn1	T	C	1: 135,959,901 (GRCm38)	Q2475R	probably damaging	Het
Il6st	T	C	13: 112,504,272 (GRCm38)	S800P	possibly damaging	Het
Iqub	G	A	6: 24,501,261 (GRCm38)		probably benign	Het
Itgb1	A	T	8: 128,722,372 (GRCm38)		probably benign	Het
Kctd21	G	A	7: 97,347,601 (GRCm38)	E94K	probably benign	Het
Kir3dl2	A	G	X: 136,453,511 (GRCm38)	V233A	possibly damaging	Het
Klra2	A	T	6: 131,220,224 (GRCm38)	C271S	probably damaging	Het
Lacc1	A	T	14: 77,029,621 (GRCm38)	C401S	probably damaging	Het
Lmna	T	C	3: 88,482,578 (GRCm38)	E580G	probably damaging	Het
Matn2	A	G	15: 34,345,150 (GRCm38)	Y101C	probably damaging	Het
Mrps16	G	A	14: 20,391,389 (GRCm38)	R116*	probably null	Het
Ndrg2	G	T	14: 51,906,217 (GRCm38)	R333S	probably damaging	Het
Nf2	A	G	11: 4,782,194 (GRCm38)	I507T	possibly damaging	Het
Nktr	A	G	9: 121,749,290 (GRCm38)		probably benign	Het
Nkx3-1	G	A	14: 69,191,006 (GRCm38)		probably benign	Het
Npat	T	C	9: 53,556,481 (GRCm38)		probably null	Het
Nrxn1	T	C	17: 90,565,373 (GRCm38)	N1047S	probably damaging	Het
Nup210	A	T	6: 91,026,929 (GRCm38)	I1402N	possibly damaging	Het
Olfr1168	G	T	2: 88,185,280 (GRCm38)	M134I	possibly damaging	Het
Parp14	G	A	16: 35,856,760 (GRCm38)	A946V	probably benign	Het
Pdcl2	C	T	5: 76,312,481 (GRCm38)	S182N	probably benign	Het
Pdia3	G	A	2: 121,432,377 (GRCm38)	G275S	probably damaging	Het
Pla2g4a	A	G	1: 149,840,704 (GRCm38)	F669L	probably benign	Het
Plekha8	A	G	6: 54,629,820 (GRCm38)	K367E	probably damaging	Het
Pola1	A	G	X: 93,488,087 (GRCm38)		probably benign	Het
Ppm1d	C	T	11: 85,345,877 (GRCm38)	T494I	probably benign	Het
Pramef25	T	A	4: 143,949,883 (GRCm38)	Y217F	probably benign	Het
Prl6a1	A	T	13: 27,314,194 (GRCm38)		probably benign	Het
Ptprg	T	A	14: 12,154,131 (GRCm38)	S617R	probably benign	Het
R3hdm2	G	A	10: 127,444,444 (GRCm38)	G45D	probably damaging	Het
Rev1	T	A	1: 38,059,123 (GRCm38)	R780W	probably null	Het
Rnf139	T	A	15: 58,899,827 (GRCm38)	F567Y	probably damaging	Het
Scarf1	T	C	11: 75,514,348 (GRCm38)	V71A	probably damaging	Het
Shtn1	A	G	19: 58,999,940 (GRCm38)	S438P	probably damaging	Het
Slc30a3	T	A	5: 31,088,723 (GRCm38)	H221L	probably benign	Het
Smo	A	C	6: 29,753,604 (GRCm38)	I160L	possibly damaging	Het
Snapc5	A	T	9: 64,179,300 (GRCm38)		probably benign	Het
Snf8	G	T	11: 96,034,973 (GRCm38)		probably benign	Het
Spata31d1a	T	C	13: 59,702,431 (GRCm38)	S628G	probably benign	Het
Sphkap	A	T	1: 83,280,424 (GRCm38)	D199E	probably damaging	Het
Stxbp5l	T	C	16: 37,204,521 (GRCm38)	T572A	possibly damaging	Het
Thada	C	A	17: 84,416,303 (GRCm38)	V1108L	possibly damaging	Het
Tln1	T	C	4: 43,547,756 (GRCm38)	Q735R	probably benign	Het
Tmem29	C	T	X: 150,398,364 (GRCm38)	A144T	probably benign	Het
Trim24	G	A	6: 37,871,234 (GRCm38)	E42K	probably benign	Het
Trnt1	T	A	6: 106,777,908 (GRCm38)		probably benign	Het
Ttbk2	A	T	2: 120,773,872 (GRCm38)	M215K	probably damaging	Het
Ttc8	C	T	12: 98,943,459 (GRCm38)		probably benign	Het
Ube3c	A	G	5: 29,590,928 (GRCm38)	Y105C	probably damaging	Het
Unc13c	A	G	9: 73,530,983 (GRCm38)		probably benign	Het
Usp36	A	G	11: 118,263,028 (GRCm38)		probably benign	Het
Vmn2r102	T	C	17: 19,678,844 (GRCm38)	S483P	possibly damaging	Het
Wdr95	A	G	5: 149,588,130 (GRCm38)	T432A	probably damaging	Het
Wnk1	G	T	6: 119,926,683 (GRCm38)	P2523H	probably damaging	Het
Xpo4	A	G	14: 57,638,208 (GRCm38)		probably benign	Het
Zar1	G	T	5: 72,580,543 (GRCm38)	P71Q	probably damaging	Het
Zbtb41	T	C	1: 139,423,610 (GRCm38)	Y154H	probably benign	Het
Zer1	G	T	2: 30,104,797 (GRCm38)		probably benign	Het
Zfp454	A	G	11: 50,874,185 (GRCm38)	F140S	probably benign	Het

Other mutations in Fryl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00819:Fryl	APN	5	73,148,108 (GRCm38)	missense	possibly damaging	0.92
IGL01518:Fryl	APN	5	73,086,962 (GRCm38)	missense	possibly damaging	0.76
IGL01545:Fryl	APN	5	73,054,597 (GRCm38)	missense	probably damaging	1.00
IGL01646:Fryl	APN	5	73,022,501 (GRCm38)	critical splice donor site	probably null
IGL01938:Fryl	APN	5	73,122,364 (GRCm38)	missense	probably damaging	0.98
IGL01962:Fryl	APN	5	73,032,791 (GRCm38)	missense	possibly damaging	0.62
IGL02064:Fryl	APN	5	73,124,769 (GRCm38)	unclassified	probably benign
IGL02148:Fryl	APN	5	73,075,959 (GRCm38)	missense	probably benign	0.35
IGL02418:Fryl	APN	5	73,110,176 (GRCm38)	splice site	probably benign
IGL02431:Fryl	APN	5	73,098,308 (GRCm38)	missense	probably benign	0.02
IGL02513:Fryl	APN	5	73,065,293 (GRCm38)	missense	probably damaging	1.00
IGL02557:Fryl	APN	5	73,098,393 (GRCm38)	missense	probably damaging	1.00
IGL02625:Fryl	APN	5	73,069,877 (GRCm38)	intron	probably benign
IGL02642:Fryl	APN	5	73,095,466 (GRCm38)	missense	probably benign
IGL02657:Fryl	APN	5	73,054,860 (GRCm38)	missense	probably benign	0.01
IGL02706:Fryl	APN	5	73,093,163 (GRCm38)	missense	probably benign	0.45
IGL03022:Fryl	APN	5	73,059,383 (GRCm38)	missense	possibly damaging	0.82
IGL03144:Fryl	APN	5	73,101,455 (GRCm38)	missense	probably null	0.22
IGL03155:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03183:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03275:Fryl	APN	5	73,148,033 (GRCm38)	missense	possibly damaging	0.47
IGL03310:Fryl	APN	5	73,136,316 (GRCm38)	splice site	probably benign
IGL03341:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03343:Fryl	APN	5	73,076,695 (GRCm38)	missense	probably benign
IGL03350:Fryl	APN	5	73,133,306 (GRCm38)	missense	probably damaging	0.99
IGL03357:Fryl	APN	5	73,054,059 (GRCm38)	missense	probably damaging	1.00
IGL03374:Fryl	APN	5	73,110,281 (GRCm38)	splice site	probably benign
IGL03375:Fryl	APN	5	73,088,449 (GRCm38)	missense	possibly damaging	0.91
bedeviled	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
Besotted	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0062:Fryl	UTSW	5	73,022,278 (GRCm38)	missense	probably benign	0.02
R0308:Fryl	UTSW	5	73,041,604 (GRCm38)	splice site	probably benign
R0312:Fryl	UTSW	5	73,072,888 (GRCm38)	missense	probably damaging	1.00
R0415:Fryl	UTSW	5	73,098,414 (GRCm38)	missense	probably damaging	0.99
R0440:Fryl	UTSW	5	73,086,972 (GRCm38)	missense	possibly damaging	0.91
R0446:Fryl	UTSW	5	73,097,417 (GRCm38)	missense	possibly damaging	0.91
R0566:Fryl	UTSW	5	73,064,497 (GRCm38)	splice site	probably benign
R0567:Fryl	UTSW	5	73,065,391 (GRCm38)	missense	possibly damaging	0.50
R0619:Fryl	UTSW	5	73,068,731 (GRCm38)	missense	probably benign	0.22
R0654:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0658:Fryl	UTSW	5	73,065,359 (GRCm38)	missense	probably damaging	1.00
R0707:Fryl	UTSW	5	73,083,372 (GRCm38)	missense	probably benign	0.17
R0744:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0745:Fryl	UTSW	5	73,071,126 (GRCm38)	missense	probably damaging	0.96
R0833:Fryl	UTSW	5	73,089,081 (GRCm38)	unclassified	probably benign
R0885:Fryl	UTSW	5	73,089,196 (GRCm38)	missense	probably damaging	0.97
R0894:Fryl	UTSW	5	73,041,332 (GRCm38)	splice site	probably benign
R1076:Fryl	UTSW	5	73,124,673 (GRCm38)	unclassified	probably benign
R1241:Fryl	UTSW	5	73,110,271 (GRCm38)	missense	probably damaging	1.00
R1241:Fryl	UTSW	5	73,064,925 (GRCm38)	splice site	probably benign
R1394:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1395:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R1608:Fryl	UTSW	5	73,074,751 (GRCm38)	nonsense	probably null
R1664:Fryl	UTSW	5	73,059,435 (GRCm38)	missense	probably damaging	1.00
R1745:Fryl	UTSW	5	73,032,861 (GRCm38)	splice site	probably benign
R1937:Fryl	UTSW	5	73,133,367 (GRCm38)	missense	probably damaging	1.00
R1969:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R1993:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R1994:Fryl	UTSW	5	73,108,493 (GRCm38)	missense	probably damaging	1.00
R2029:Fryl	UTSW	5	73,022,122 (GRCm38)	nonsense	probably null
R2036:Fryl	UTSW	5	73,107,962 (GRCm38)	critical splice donor site	probably null
R2036:Fryl	UTSW	5	73,022,544 (GRCm38)	missense	probably benign
R2088:Fryl	UTSW	5	73,065,461 (GRCm38)	missense	probably benign	0.02
R2105:Fryl	UTSW	5	73,122,299 (GRCm38)	missense	probably benign
R2106:Fryl	UTSW	5	73,098,331 (GRCm38)	missense	probably damaging	1.00
R2186:Fryl	UTSW	5	73,064,975 (GRCm38)	missense	probably damaging	1.00
R2239:Fryl	UTSW	5	73,108,547 (GRCm38)	missense	probably damaging	0.99
R2256:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2257:Fryl	UTSW	5	73,072,844 (GRCm38)	missense	possibly damaging	0.47
R2280:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2281:Fryl	UTSW	5	73,041,364 (GRCm38)	missense	possibly damaging	0.47
R2911:Fryl	UTSW	5	73,050,456 (GRCm38)	missense	probably damaging	0.99
R3019:Fryl	UTSW	5	73,082,850 (GRCm38)	missense	probably benign	0.01
R3416:Fryl	UTSW	5	73,108,074 (GRCm38)	missense	possibly damaging	0.84
R3783:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3787:Fryl	UTSW	5	73,101,476 (GRCm38)	missense	probably benign
R3837:Fryl	UTSW	5	73,071,265 (GRCm38)	missense	probably benign	0.03
R3969:Fryl	UTSW	5	73,112,423 (GRCm38)	missense	probably damaging	0.97
R4387:Fryl	UTSW	5	73,086,560 (GRCm38)	missense	possibly damaging	0.91
R4502:Fryl	UTSW	5	73,088,397 (GRCm38)	missense	probably damaging	1.00
R4658:Fryl	UTSW	5	73,081,053 (GRCm38)	missense	probably damaging	1.00
R4664:Fryl	UTSW	5	73,090,679 (GRCm38)	missense	possibly damaging	0.80
R4690:Fryl	UTSW	5	73,100,293 (GRCm38)	missense	probably benign
R4700:Fryl	UTSW	5	73,065,538 (GRCm38)	missense	possibly damaging	0.88
R4709:Fryl	UTSW	5	73,080,972 (GRCm38)	missense	probably benign	0.03
R4807:Fryl	UTSW	5	73,041,362 (GRCm38)	missense	probably benign	0.00
R4912:Fryl	UTSW	5	73,068,782 (GRCm38)	frame shift	probably null
R4948:Fryl	UTSW	5	73,089,130 (GRCm38)	missense	probably benign	0.08
R4959:Fryl	UTSW	5	73,035,058 (GRCm38)	missense	probably benign	0.00
R5062:Fryl	UTSW	5	73,075,893 (GRCm38)	missense	possibly damaging	0.89
R5067:Fryl	UTSW	5	73,057,755 (GRCm38)	missense	probably benign	0.13
R5071:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5072:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5073:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5074:Fryl	UTSW	5	73,074,767 (GRCm38)	missense	probably damaging	0.99
R5139:Fryl	UTSW	5	73,090,718 (GRCm38)	missense	probably damaging	1.00
R5172:Fryl	UTSW	5	73,101,673 (GRCm38)	missense	possibly damaging	0.95
R5187:Fryl	UTSW	5	73,086,600 (GRCm38)	missense	possibly damaging	0.95
R5272:Fryl	UTSW	5	73,065,136 (GRCm38)	nonsense	probably null
R5275:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5295:Fryl	UTSW	5	73,112,791 (GRCm38)	missense	probably damaging	1.00
R5344:Fryl	UTSW	5	73,104,774 (GRCm38)	missense	probably damaging	1.00
R5355:Fryl	UTSW	5	73,073,904 (GRCm38)	missense	probably damaging	1.00
R5716:Fryl	UTSW	5	73,100,465 (GRCm38)	missense	probably benign
R5778:Fryl	UTSW	5	73,072,778 (GRCm38)	missense	probably damaging	1.00
R5810:Fryl	UTSW	5	73,090,755 (GRCm38)	missense	probably benign	0.06
R5934:Fryl	UTSW	5	73,090,717 (GRCm38)	missense	probably damaging	1.00
R5948:Fryl	UTSW	5	73,097,372 (GRCm38)	critical splice donor site	probably null
R6005:Fryl	UTSW	5	73,083,295 (GRCm38)	missense	probably damaging	1.00
R6026:Fryl	UTSW	5	73,099,997 (GRCm38)	missense	probably benign	0.04
R6045:Fryl	UTSW	5	73,118,551 (GRCm38)	missense	probably damaging	0.99
R6185:Fryl	UTSW	5	73,112,788 (GRCm38)	missense	probably benign	0.43
R6247:Fryl	UTSW	5	73,065,481 (GRCm38)	missense	probably damaging	0.98
R6294:Fryl	UTSW	5	73,191,759 (GRCm38)	intron	probably benign
R6310:Fryl	UTSW	5	73,191,761 (GRCm38)	intron	probably benign
R6429:Fryl	UTSW	5	73,090,751 (GRCm38)	missense	possibly damaging	0.84
R6568:Fryl	UTSW	5	73,059,516 (GRCm38)	missense	probably damaging	1.00
R6636:Fryl	UTSW	5	73,133,312 (GRCm38)	missense	probably benign	0.01
R6664:Fryl	UTSW	5	73,132,481 (GRCm38)	missense	probably damaging	1.00
R6732:Fryl	UTSW	5	73,054,781 (GRCm38)	missense	probably damaging	1.00
R6750:Fryl	UTSW	5	73,022,232 (GRCm38)	missense	probably damaging	1.00
R6805:Fryl	UTSW	5	73,065,094 (GRCm38)	missense	probably benign	0.03
R6823:Fryl	UTSW	5	73,065,217 (GRCm38)	missense	probably damaging	0.99
R6855:Fryl	UTSW	5	73,059,500 (GRCm38)	missense	probably damaging	1.00
R6858:Fryl	UTSW	5	73,065,032 (GRCm38)	missense	probably damaging	1.00
R6868:Fryl	UTSW	5	73,068,803 (GRCm38)	missense	probably damaging	1.00
R6898:Fryl	UTSW	5	73,022,142 (GRCm38)	missense	probably damaging	0.96
R6908:Fryl	UTSW	5	73,022,211 (GRCm38)	missense	probably damaging	1.00
R6958:Fryl	UTSW	5	73,073,929 (GRCm38)	missense	possibly damaging	0.89
R6980:Fryl	UTSW	5	73,050,430 (GRCm38)	missense	probably benign	0.06
R7036:Fryl	UTSW	5	73,055,608 (GRCm38)	missense	probably benign	0.03
R7065:Fryl	UTSW	5	73,090,756 (GRCm38)	missense	probably damaging	0.96
R7097:Fryl	UTSW	5	73,073,908 (GRCm38)	missense	probably benign	0.31
R7171:Fryl	UTSW	5	73,122,310 (GRCm38)	missense	probably damaging	0.97
R7191:Fryl	UTSW	5	73,072,912 (GRCm38)	missense	probably damaging	1.00
R7207:Fryl	UTSW	5	73,065,095 (GRCm38)	missense	probably benign
R7236:Fryl	UTSW	5	73,108,478 (GRCm38)	missense	possibly damaging	0.66
R7334:Fryl	UTSW	5	73,047,496 (GRCm38)	splice site	probably null
R7425:Fryl	UTSW	5	73,104,748 (GRCm38)	missense	probably damaging	1.00
R7452:Fryl	UTSW	5	73,023,988 (GRCm38)	missense	probably damaging	1.00
R7479:Fryl	UTSW	5	73,097,561 (GRCm38)	missense	possibly damaging	0.71
R7535:Fryl	UTSW	5	73,098,196 (GRCm38)	missense	probably benign	0.15
R7538:Fryl	UTSW	5	73,022,676 (GRCm38)	missense	probably benign	0.09
R7544:Fryl	UTSW	5	73,081,039 (GRCm38)	missense	probably benign
R7548:Fryl	UTSW	5	73,191,762 (GRCm38)	missense	unknown
R7565:Fryl	UTSW	5	73,033,720 (GRCm38)	missense	probably benign	0.18
R7572:Fryl	UTSW	5	73,088,396 (GRCm38)	missense	possibly damaging	0.91
R7582:Fryl	UTSW	5	73,022,500 (GRCm38)	critical splice donor site	probably null
R7630:Fryl	UTSW	5	73,110,245 (GRCm38)	missense	possibly damaging	0.62
R7774:Fryl	UTSW	5	73,083,384 (GRCm38)	missense	probably benign	0.12
R7777:Fryl	UTSW	5	73,071,298 (GRCm38)	missense	probably damaging	0.98
R7917:Fryl	UTSW	5	73,054,532 (GRCm38)	missense	probably damaging	1.00
R7920:Fryl	UTSW	5	73,101,807 (GRCm38)	splice site	probably null
R8110:Fryl	UTSW	5	73,133,277 (GRCm38)	missense	probably benign	0.10
R8120:Fryl	UTSW	5	73,071,184 (GRCm38)	missense	probably benign	0.01
R8143:Fryl	UTSW	5	73,050,339 (GRCm38)	missense	probably benign	0.00
R8207:Fryl	UTSW	5	73,100,500 (GRCm38)	splice site	probably null
R8263:Fryl	UTSW	5	73,081,005 (GRCm38)	missense	probably damaging	1.00
R8350:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8359:Fryl	UTSW	5	73,075,933 (GRCm38)	missense	probably benign	0.39
R8387:Fryl	UTSW	5	73,136,320 (GRCm38)	critical splice donor site	probably null
R8403:Fryl	UTSW	5	73,118,447 (GRCm38)	makesense	probably null
R8450:Fryl	UTSW	5	73,068,730 (GRCm38)	missense	probably benign
R8514:Fryl	UTSW	5	73,085,356 (GRCm38)	missense	probably benign
R8536:Fryl	UTSW	5	73,100,353 (GRCm38)	missense	probably damaging	0.99
R8703:Fryl	UTSW	5	73,090,654 (GRCm38)	missense	probably damaging	0.99
R8708:Fryl	UTSW	5	73,132,562 (GRCm38)	missense	probably benign	0.01
R8783:Fryl	UTSW	5	73,068,842 (GRCm38)	missense	probably benign	0.45
R9028:Fryl	UTSW	5	73,098,266 (GRCm38)	missense	probably benign	0.18
R9045:Fryl	UTSW	5	73,024,775 (GRCm38)	missense
R9063:Fryl	UTSW	5	73,081,003 (GRCm38)	missense	possibly damaging	0.70
R9096:Fryl	UTSW	5	73,108,577 (GRCm38)	missense	probably benign	0.01
R9244:Fryl	UTSW	5	73,191,519 (GRCm38)	intron	probably benign
R9345:Fryl	UTSW	5	73,050,411 (GRCm38)	missense	probably benign
R9381:Fryl	UTSW	5	73,083,294 (GRCm38)	missense	probably benign	0.24
R9386:Fryl	UTSW	5	73,191,809 (GRCm38)	missense	unknown
R9401:Fryl	UTSW	5	73,065,220 (GRCm38)	nonsense	probably null
R9497:Fryl	UTSW	5	73,057,791 (GRCm38)	missense
R9514:Fryl	UTSW	5	73,104,772 (GRCm38)	missense	probably damaging	1.00
R9570:Fryl	UTSW	5	73,022,155 (GRCm38)	missense	probably benign	0.02
R9654:Fryl	UTSW	5	73,118,458 (GRCm38)	missense	probably benign
R9665:Fryl	UTSW	5	73,064,956 (GRCm38)	missense	probably damaging	1.00
R9685:Fryl	UTSW	5	73,059,536 (GRCm38)	missense	probably damaging	0.99
R9798:Fryl	UTSW	5	73,035,059 (GRCm38)	missense	probably benign
Z1088:Fryl	UTSW	5	73,090,738 (GRCm38)	missense	probably damaging	1.00
Z1088:Fryl	UTSW	5	73,090,709 (GRCm38)	missense	probably damaging	0.99
Z1176:Fryl	UTSW	5	73,072,837 (GRCm38)	missense	probably benign
Z1177:Fryl	UTSW	5	73,041,595 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- GAGACTACAGTAAGCAAGGCTGCCAC -3'
(R):5'- TCTCCTACAAGTACCTGTTCAGCCAAG -3'

Sequencing Primer
(F):5'- CACCGAGCCTCATCAGTG -3'
(R):5'- tctttttcttcttctttctggttttg -3'

Posted On

2013-07-11