Mutagenetix > Incidental Mutation

Incidental Mutation 'R6971:Hmcn2'

542233

Institutional Source

Beutler Lab

Gene Symbol

Hmcn2

Ensembl Gene

ENSMUSG00000055632

Gene Name

hemicentin 2

Synonyms

MMRRC Submission

045081-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R6971 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

31314415-31460738 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 31432321 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 4133 (E4133K)

Ref Sequence

ENSEMBL: ENSMUSP00000109160 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000113532] [ENSMUST00000226996]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000113532
AA Change: E4133K

PolyPhen 2 Score 0.022 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000109160
Gene: ENSMUSG00000055632
AA Change: E4133K

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
VWA	37	211	1.21e-1	SMART
Blast:IG_like	263	340	2e-38	BLAST
IG	434	515	7.36e-2	SMART
IGc2	530	595	1.91e-9	SMART
IGc2	621	685	4.81e-15	SMART
IGc2	711	773	1.09e-13	SMART
IGc2	799	866	2.72e-14	SMART
IGc2	894	959	1.95e-15	SMART
IGc2	985	1049	5e-13	SMART
IGc2	1082	1147	1.09e-13	SMART
low complexity region	1151	1169	N/A	INTRINSIC
IGc2	1173	1232	7.07e-13	SMART
IGc2	1260	1326	4.31e-17	SMART
IGc2	1354	1428	3e-16	SMART
IGc2	1456	1522	1.82e-15	SMART
IGc2	1550	1615	2.7e-18	SMART
IGc2	1644	1708	1.3e-11	SMART
IGc2	1736	1801	6.69e-14	SMART
IG	1826	1917	2.31e0	SMART
IGc2	1932	1997	4.62e-17	SMART
IGc2	2024	2091	3.25e-12	SMART
IGc2	2117	2182	1.28e-10	SMART
IGc2	2209	2276	3.76e-8	SMART
IGc2	2305	2370	2.6e-11	SMART
IGc2	2399	2464	1.32e-12	SMART
IGc2	2492	2557	2.06e-14	SMART
IGc2	2588	2653	3.9e-15	SMART
IGc2	2686	2751	2.64e-12	SMART
IGc2	2797	2862	9.05e-11	SMART
IGc2	2892	2957	4.7e-9	SMART
IGc2	2984	3049	1.44e-13	SMART
IGc2	3079	3144	9.33e-13	SMART
IGc2	3171	3236	3.79e-13	SMART
IGc2	3264	3331	1.85e-16	SMART
IGc2	3360	3425	9.61e-15	SMART
low complexity region	3433	3445	N/A	INTRINSIC
IGc2	3453	3514	5.83e-14	SMART
IGc2	3542	3600	1.76e-8	SMART
low complexity region	3613	3627	N/A	INTRINSIC
IGc2	3628	3693	5.2e-11	SMART
IGc2	3719	3784	2.64e-12	SMART
IGc2	3810	3877	3.35e-5	SMART
IGc2	3903	3968	3.73e-12	SMART
IGc2	3994	4058	4.39e-9	SMART
IGc2	4084	4149	1.79e-14	SMART
low complexity region	4157	4169	N/A	INTRINSIC
IGc2	4175	4238	9.33e-13	SMART
IGc2	4265	4329	7.22e-19	SMART
IGc2	4355	4419	1.59e-15	SMART
Pfam:G2F	4431	4613	1.7e-56	PFAM
EGF_CA	4668	4708	5.78e-11	SMART
EGF_CA	4709	4753	9.39e-11	SMART
EGF_CA	4754	4796	7.69e-7	SMART
EGF_CA	4797	4837	2.19e-11	SMART
EGF_CA	4904	4943	6.74e-12	SMART
EGF_like	4944	4989	1.87e-5	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000226996

Meta Mutation Damage Score

0.0842

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.2%
20x: 97.1%

Validation Efficiency

96% (47/49)

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700113H08Rik	A	T	10: 87,165,041 (GRCm38)	T32S	possibly damaging	Het
2210408I21Rik	A	G	13: 77,193,187 (GRCm38)	S52G	possibly damaging	Het
Aadacl4	G	A	4: 144,622,733 (GRCm38)	V187M	probably damaging	Het
Adprhl1	T	A	8: 13,223,476 (GRCm38)	Q1094L	probably benign	Het
Amigo1	C	T	3: 108,188,136 (GRCm38)	S317L	probably benign	Het
Brca1	A	G	11: 101,534,005 (GRCm38)	F32L	probably benign	Het
C1qtnf7	T	A	5: 43,609,050 (GRCm38)		probably null	Het
Ccdc88c	A	T	12: 100,954,227 (GRCm38)	D378E	probably damaging	Het
Ccdc97	G	T	7: 25,714,959 (GRCm38)	Y123*	probably null	Het
Cdk10	T	A	8: 123,227,674 (GRCm38)	M46K	probably damaging	Het
Dsc3	A	G	18: 19,966,218 (GRCm38)		probably null	Het
Ephx3	T	A	17: 32,188,203 (GRCm38)	N254Y	possibly damaging	Het
Fnip2	G	A	3: 79,481,121 (GRCm38)	R768*	probably null	Het
Glra1	A	T	11: 55,536,499 (GRCm38)	Y3*	probably null	Het
Gltpd2	A	G	11: 70,520,464 (GRCm38)	T194A	probably damaging	Het
Hectd1	A	T	12: 51,748,743 (GRCm38)	L2301*	probably null	Het
Hmcn1	T	A	1: 150,993,051 (GRCm38)	M1L	probably benign	Het
Hps3	A	T	3: 20,011,535 (GRCm38)	L714I	probably damaging	Het
Igfbpl1	A	G	4: 45,816,333 (GRCm38)	V164A	possibly damaging	Het
Ip6k2	T	C	9: 108,797,311 (GRCm38)		probably benign	Het
Itgb8	A	T	12: 119,190,631 (GRCm38)	Y224N	probably damaging	Het
Kcnt2	T	C	1: 140,512,908 (GRCm38)	L624S	probably benign	Het
Mdga2	A	G	12: 66,550,561 (GRCm38)	Y720H	probably damaging	Het
Mier2	G	A	10: 79,542,429 (GRCm38)	H385Y	possibly damaging	Het
Msl2	T	C	9: 101,100,843 (GRCm38)	F139L	probably benign	Het
Nuggc	T	C	14: 65,608,856 (GRCm38)	V72A	probably benign	Het
Olfr826	A	T	10: 130,180,769 (GRCm38)	V37E	possibly damaging	Het
Pde2a	T	A	7: 101,510,313 (GRCm38)	Y783*	probably null	Het
Pfkfb2	A	G	1: 130,700,796 (GRCm38)	Y358H	probably damaging	Het
Pou2f1	A	G	1: 165,931,689 (GRCm38)	S23P	probably damaging	Het
Prrc2a	T	C	17: 35,159,501 (GRCm38)		probably null	Het
Prss36	T	C	7: 127,945,238 (GRCm38)	T92A	probably benign	Het
Rnft2	A	G	5: 118,194,570 (GRCm38)		probably benign	Het
Sbno2	A	G	10: 80,060,034 (GRCm38)	V971A	possibly damaging	Het
Sec63	A	G	10: 42,783,442 (GRCm38)	E42G	probably damaging	Het
Setdb2	A	T	14: 59,415,740 (GRCm38)	L371Q	probably damaging	Het
Shprh	A	T	10: 11,166,693 (GRCm38)	I807F	probably damaging	Het
Slc3a2	T	C	19: 8,709,610 (GRCm38)		probably null	Het
Srbd1	T	C	17: 86,099,290 (GRCm38)	I556V	possibly damaging	Het
Stim2	T	A	5: 54,118,299 (GRCm38)	C605*	probably null	Het
Tecrl	T	C	5: 83,354,802 (GRCm38)	T67A	possibly damaging	Het
Ttc33	C	A	15: 5,212,042 (GRCm38)	A116E	probably damaging	Het
Ttn	G	A	2: 76,942,049 (GRCm38)	T2503I	possibly damaging	Het
Ubp1	T	C	9: 113,972,763 (GRCm38)	I468T	probably damaging	Het
Urgcp	A	T	11: 5,718,115 (GRCm38)	H74Q	probably benign	Het
Vcan	A	G	13: 89,678,133 (GRCm38)	I2224T	probably damaging	Het
Vmn1r8	T	A	6: 57,036,415 (GRCm38)	N150K	probably damaging	Het
Vmn2r3	A	G	3: 64,259,247 (GRCm38)	M821T	probably damaging	Het
Zfp777	C	A	6: 48,024,691 (GRCm38)	A866S	probably damaging	Het

Other mutations in Hmcn2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00965:Hmcn2	APN	2	31,343,096 (GRCm38)	missense	probably damaging	1.00
IGL00966:Hmcn2	APN	2	31,428,994 (GRCm38)	missense	probably damaging	0.97
IGL00973:Hmcn2	APN	2	31,383,821 (GRCm38)	intron	probably benign
IGL01364:Hmcn2	APN	2	31,361,814 (GRCm38)	nonsense	probably null
IGL01486:Hmcn2	APN	2	31,336,621 (GRCm38)	missense	probably damaging	1.00
IGL01530:Hmcn2	APN	2	31,354,264 (GRCm38)	missense	possibly damaging	0.85
IGL01550:Hmcn2	APN	2	31,424,252 (GRCm38)	missense	possibly damaging	0.84
IGL01710:Hmcn2	APN	2	31,343,102 (GRCm38)	missense	probably damaging	1.00
IGL01764:Hmcn2	APN	2	31,405,630 (GRCm38)	missense	possibly damaging	0.93
IGL01924:Hmcn2	APN	2	31,398,917 (GRCm38)	missense	probably benign	0.00
IGL02003:Hmcn2	APN	2	31,428,982 (GRCm38)	missense	possibly damaging	0.90
IGL02117:Hmcn2	APN	2	31,457,173 (GRCm38)	missense	possibly damaging	0.75
IGL02205:Hmcn2	APN	2	31,400,127 (GRCm38)	missense	probably damaging	1.00
IGL02273:Hmcn2	APN	2	31,424,377 (GRCm38)	missense	probably benign	0.06
IGL02313:Hmcn2	APN	2	31,453,605 (GRCm38)	missense	possibly damaging	0.68
IGL02326:Hmcn2	APN	2	31,450,952 (GRCm38)	missense	probably damaging	0.97
IGL02486:Hmcn2	APN	2	31,420,095 (GRCm38)	missense	probably damaging	0.98
IGL02551:Hmcn2	APN	2	31,454,811 (GRCm38)	missense	possibly damaging	0.83
IGL02695:Hmcn2	APN	2	31,408,973 (GRCm38)	missense	possibly damaging	0.87
IGL02725:Hmcn2	APN	2	31,405,528 (GRCm38)	missense	probably damaging	1.00
IGL02792:Hmcn2	APN	2	31,346,590 (GRCm38)	missense	probably damaging	1.00
IGL02882:Hmcn2	APN	2	31,413,367 (GRCm38)	nonsense	probably null
IGL03003:Hmcn2	APN	2	31,433,486 (GRCm38)	missense	probably damaging	0.98
IGL03067:Hmcn2	APN	2	31,346,630 (GRCm38)	missense	probably damaging	1.00
IGL03137:Hmcn2	APN	2	31,362,230 (GRCm38)	missense	probably damaging	0.98
IGL03220:Hmcn2	APN	2	31,346,621 (GRCm38)	missense	possibly damaging	0.94
IGL03411:Hmcn2	APN	2	31,346,637 (GRCm38)	missense	possibly damaging	0.83
PIT4544001:Hmcn2	UTSW	2	31,428,250 (GRCm38)	missense	probably damaging	0.98
R0044:Hmcn2	UTSW	2	31,412,508 (GRCm38)	missense	probably damaging	0.98
R0044:Hmcn2	UTSW	2	31,412,508 (GRCm38)	missense	probably damaging	0.98
R0048:Hmcn2	UTSW	2	31,428,237 (GRCm38)	missense	possibly damaging	0.92
R0048:Hmcn2	UTSW	2	31,428,237 (GRCm38)	missense	possibly damaging	0.92
R0078:Hmcn2	UTSW	2	31,388,344 (GRCm38)	missense	probably damaging	1.00
R0090:Hmcn2	UTSW	2	31,426,198 (GRCm38)	missense	probably damaging	1.00
R0173:Hmcn2	UTSW	2	31,438,331 (GRCm38)	critical splice donor site	probably null
R0257:Hmcn2	UTSW	2	31,369,164 (GRCm38)	splice site	probably benign
R0266:Hmcn2	UTSW	2	31,445,353 (GRCm38)	splice site	probably benign
R0266:Hmcn2	UTSW	2	31,394,827 (GRCm38)	missense	probably benign	0.03
R0326:Hmcn2	UTSW	2	31,423,225 (GRCm38)	nonsense	probably null
R0366:Hmcn2	UTSW	2	31,424,206 (GRCm38)	missense	possibly damaging	0.88
R0400:Hmcn2	UTSW	2	31,400,129 (GRCm38)	missense	probably damaging	0.98
R0412:Hmcn2	UTSW	2	31,388,247 (GRCm38)	missense	probably damaging	0.98
R0436:Hmcn2	UTSW	2	31,405,612 (GRCm38)	missense	probably damaging	1.00
R0457:Hmcn2	UTSW	2	31,415,284 (GRCm38)	critical splice donor site	probably null
R0487:Hmcn2	UTSW	2	31,386,677 (GRCm38)	missense	possibly damaging	0.60
R0568:Hmcn2	UTSW	2	31,415,236 (GRCm38)	missense	probably benign	0.02
R0755:Hmcn2	UTSW	2	31,453,160 (GRCm38)	missense	probably damaging	0.99
R0811:Hmcn2	UTSW	2	31,420,371 (GRCm38)	missense	probably damaging	0.99
R0812:Hmcn2	UTSW	2	31,420,371 (GRCm38)	missense	probably damaging	0.99
R0964:Hmcn2	UTSW	2	31,391,511 (GRCm38)	missense	probably benign	0.23
R0988:Hmcn2	UTSW	2	31,335,451 (GRCm38)	missense	probably damaging	1.00
R1484:Hmcn2	UTSW	2	31,346,495 (GRCm38)	missense	probably damaging	1.00
R1509:Hmcn2	UTSW	2	31,314,479 (GRCm38)	missense	possibly damaging	0.86
R1535:Hmcn2	UTSW	2	31,420,407 (GRCm38)	missense	possibly damaging	0.91
R1574:Hmcn2	UTSW	2	31,404,887 (GRCm38)	missense	probably damaging	0.97
R1574:Hmcn2	UTSW	2	31,404,887 (GRCm38)	missense	probably damaging	0.97
R1600:Hmcn2	UTSW	2	31,430,787 (GRCm38)	missense	probably damaging	0.98
R1623:Hmcn2	UTSW	2	31,458,039 (GRCm38)	missense	possibly damaging	0.84
R1692:Hmcn2	UTSW	2	31,450,844 (GRCm38)	missense	possibly damaging	0.47
R1719:Hmcn2	UTSW	2	31,354,721 (GRCm38)	missense	probably damaging	1.00
R1747:Hmcn2	UTSW	2	31,457,985 (GRCm38)	missense	probably benign	0.00
R1756:Hmcn2	UTSW	2	31,396,120 (GRCm38)	missense	probably damaging	0.99
R1763:Hmcn2	UTSW	2	31,314,590 (GRCm38)	missense	probably damaging	1.00
R1815:Hmcn2	UTSW	2	31,393,043 (GRCm38)	missense	probably damaging	0.97
R1822:Hmcn2	UTSW	2	31,383,692 (GRCm38)	missense	probably damaging	0.99
R1858:Hmcn2	UTSW	2	31,415,283 (GRCm38)	critical splice donor site	probably null
R1895:Hmcn2	UTSW	2	31,405,635 (GRCm38)	missense	probably damaging	0.99
R1908:Hmcn2	UTSW	2	31,411,910 (GRCm38)	critical splice donor site	probably null
R1946:Hmcn2	UTSW	2	31,405,635 (GRCm38)	missense	probably damaging	0.99
R1966:Hmcn2	UTSW	2	31,389,329 (GRCm38)	missense	probably damaging	0.99
R2007:Hmcn2	UTSW	2	31,438,255 (GRCm38)	missense	possibly damaging	0.91
R2050:Hmcn2	UTSW	2	31,335,436 (GRCm38)	missense	probably damaging	1.00
R2055:Hmcn2	UTSW	2	31,378,282 (GRCm38)	missense	probably benign	0.33
R2097:Hmcn2	UTSW	2	31,380,419 (GRCm38)	missense	probably damaging	1.00
R2145:Hmcn2	UTSW	2	31,333,931 (GRCm38)	splice site	probably benign
R2155:Hmcn2	UTSW	2	31,460,349 (GRCm38)	missense	possibly damaging	0.68
R2170:Hmcn2	UTSW	2	31,380,281 (GRCm38)	missense	probably benign	0.08
R2188:Hmcn2	UTSW	2	31,419,935 (GRCm38)	missense	probably benign	0.14
R2208:Hmcn2	UTSW	2	31,380,297 (GRCm38)	missense	probably damaging	1.00
R2217:Hmcn2	UTSW	2	31,350,574 (GRCm38)	missense	probably benign	0.02
R2407:Hmcn2	UTSW	2	31,335,412 (GRCm38)	critical splice acceptor site	probably null
R2764:Hmcn2	UTSW	2	31,388,298 (GRCm38)	missense	probably damaging	0.98
R2913:Hmcn2	UTSW	2	31,460,210 (GRCm38)	missense	possibly damaging	0.68
R2986:Hmcn2	UTSW	2	31,360,998 (GRCm38)	missense	probably damaging	1.00
R3157:Hmcn2	UTSW	2	31,400,255 (GRCm38)	missense	probably damaging	0.99
R3406:Hmcn2	UTSW	2	31,433,272 (GRCm38)	splice site	probably benign
R3429:Hmcn2	UTSW	2	31,409,144 (GRCm38)	missense	possibly damaging	0.87
R3737:Hmcn2	UTSW	2	31,336,612 (GRCm38)	nonsense	probably null
R3739:Hmcn2	UTSW	2	31,336,612 (GRCm38)	nonsense	probably null
R3771:Hmcn2	UTSW	2	31,360,896 (GRCm38)	missense	probably damaging	0.99
R3772:Hmcn2	UTSW	2	31,360,896 (GRCm38)	missense	probably damaging	0.99
R3773:Hmcn2	UTSW	2	31,360,896 (GRCm38)	missense	probably damaging	0.99
R3804:Hmcn2	UTSW	2	31,352,885 (GRCm38)	splice site	probably null
R3837:Hmcn2	UTSW	2	31,413,407 (GRCm38)	missense	probably damaging	0.99
R3838:Hmcn2	UTSW	2	31,413,407 (GRCm38)	missense	probably damaging	0.99
R3846:Hmcn2	UTSW	2	31,430,350 (GRCm38)	missense	possibly damaging	0.51
R3925:Hmcn2	UTSW	2	31,453,157 (GRCm38)	missense	probably benign	0.00
R3934:Hmcn2	UTSW	2	31,380,484 (GRCm38)	critical splice donor site	probably null
R3946:Hmcn2	UTSW	2	31,382,394 (GRCm38)	missense	possibly damaging	0.91
R4035:Hmcn2	UTSW	2	31,336,612 (GRCm38)	nonsense	probably null
R4057:Hmcn2	UTSW	2	31,400,238 (GRCm38)	missense	probably damaging	1.00
R4583:Hmcn2	UTSW	2	31,413,265 (GRCm38)	missense	possibly damaging	0.84
R4623:Hmcn2	UTSW	2	31,396,710 (GRCm38)	missense	probably damaging	1.00
R4647:Hmcn2	UTSW	2	31,399,019 (GRCm38)	missense	possibly damaging	0.82
R4668:Hmcn2	UTSW	2	31,435,792 (GRCm38)	missense	probably benign	0.40
R4669:Hmcn2	UTSW	2	31,435,792 (GRCm38)	missense	probably benign	0.40
R4687:Hmcn2	UTSW	2	31,438,285 (GRCm38)	missense	probably benign	0.14
R4735:Hmcn2	UTSW	2	31,383,775 (GRCm38)	missense	probably benign	0.06
R4772:Hmcn2	UTSW	2	31,445,314 (GRCm38)	missense	probably benign	0.02
R4866:Hmcn2	UTSW	2	31,389,391 (GRCm38)	missense	possibly damaging	0.88
R4916:Hmcn2	UTSW	2	31,360,980 (GRCm38)	missense	probably damaging	0.98
R4943:Hmcn2	UTSW	2	31,335,492 (GRCm38)	missense	probably damaging	1.00
R4967:Hmcn2	UTSW	2	31,354,164 (GRCm38)	critical splice acceptor site	probably null
R4973:Hmcn2	UTSW	2	31,344,096 (GRCm38)	missense	probably benign	0.15
R4975:Hmcn2	UTSW	2	31,393,025 (GRCm38)	missense	possibly damaging	0.88
R4994:Hmcn2	UTSW	2	31,458,055 (GRCm38)	critical splice donor site	probably null
R4997:Hmcn2	UTSW	2	31,401,708 (GRCm38)	missense	probably damaging	1.00
R5045:Hmcn2	UTSW	2	31,409,081 (GRCm38)	missense	probably damaging	1.00
R5117:Hmcn2	UTSW	2	31,458,049 (GRCm38)	missense	possibly damaging	0.95
R5151:Hmcn2	UTSW	2	31,389,443 (GRCm38)	missense	probably null
R5232:Hmcn2	UTSW	2	31,457,748 (GRCm38)	missense	probably damaging	0.99
R5237:Hmcn2	UTSW	2	31,414,716 (GRCm38)	missense	probably benign	0.01
R5288:Hmcn2	UTSW	2	31,460,321 (GRCm38)	missense	probably benign	0.11
R5375:Hmcn2	UTSW	2	31,430,441 (GRCm38)	missense	possibly damaging	0.92
R5379:Hmcn2	UTSW	2	31,409,011 (GRCm38)	missense	probably damaging	0.99
R5385:Hmcn2	UTSW	2	31,460,321 (GRCm38)	missense	probably benign	0.11
R5412:Hmcn2	UTSW	2	31,346,617 (GRCm38)	missense	possibly damaging	0.77
R5426:Hmcn2	UTSW	2	31,336,544 (GRCm38)	missense	possibly damaging	0.95
R5434:Hmcn2	UTSW	2	31,420,363 (GRCm38)	missense	probably damaging	1.00
R5441:Hmcn2	UTSW	2	31,406,416 (GRCm38)	missense	possibly damaging	0.82
R5484:Hmcn2	UTSW	2	31,393,054 (GRCm38)	nonsense	probably null
R5492:Hmcn2	UTSW	2	31,420,306 (GRCm38)	missense	probably benign	0.03
R5572:Hmcn2	UTSW	2	31,414,526 (GRCm38)	critical splice acceptor site	probably null
R5572:Hmcn2	UTSW	2	31,414,525 (GRCm38)	critical splice acceptor site	probably null
R5591:Hmcn2	UTSW	2	31,344,047 (GRCm38)	missense	probably damaging	1.00
R5614:Hmcn2	UTSW	2	31,428,303 (GRCm38)	missense	probably damaging	0.99
R5634:Hmcn2	UTSW	2	31,333,881 (GRCm38)	missense	probably damaging	1.00
R5645:Hmcn2	UTSW	2	31,420,812 (GRCm38)	missense	possibly damaging	0.92
R5716:Hmcn2	UTSW	2	31,458,738 (GRCm38)	missense	possibly damaging	0.68
R5716:Hmcn2	UTSW	2	31,336,567 (GRCm38)	missense	probably damaging	1.00
R5725:Hmcn2	UTSW	2	31,383,815 (GRCm38)	critical splice donor site	probably null
R5760:Hmcn2	UTSW	2	31,414,568 (GRCm38)	missense	possibly damaging	0.91
R5774:Hmcn2	UTSW	2	31,409,135 (GRCm38)	missense	possibly damaging	0.94
R5838:Hmcn2	UTSW	2	31,457,807 (GRCm38)	missense	probably damaging	0.99
R5899:Hmcn2	UTSW	2	31,354,673 (GRCm38)	missense	possibly damaging	0.93
R5916:Hmcn2	UTSW	2	31,396,139 (GRCm38)	missense	probably damaging	1.00
R5973:Hmcn2	UTSW	2	31,420,323 (GRCm38)	missense	probably damaging	0.99
R6002:Hmcn2	UTSW	2	31,420,309 (GRCm38)	missense	probably damaging	0.99
R6018:Hmcn2	UTSW	2	31,370,792 (GRCm38)	missense	probably benign	0.13
R6063:Hmcn2	UTSW	2	31,434,713 (GRCm38)	missense	probably benign	0.06
R6161:Hmcn2	UTSW	2	31,356,254 (GRCm38)	missense	probably benign
R6166:Hmcn2	UTSW	2	31,369,262 (GRCm38)	missense	probably damaging	1.00
R6177:Hmcn2	UTSW	2	31,420,106 (GRCm38)	nonsense	probably null
R6191:Hmcn2	UTSW	2	31,458,746 (GRCm38)	missense	probably damaging	0.99
R6195:Hmcn2	UTSW	2	31,384,115 (GRCm38)	missense	probably damaging	0.96
R6273:Hmcn2	UTSW	2	31,411,834 (GRCm38)	missense	probably damaging	0.99
R6293:Hmcn2	UTSW	2	31,335,451 (GRCm38)	missense	probably damaging	1.00
R6349:Hmcn2	UTSW	2	31,388,373 (GRCm38)	missense	probably damaging	1.00
R6395:Hmcn2	UTSW	2	31,369,257 (GRCm38)	missense	probably damaging	1.00
R6448:Hmcn2	UTSW	2	31,420,820 (GRCm38)	missense	probably benign	0.02
R6450:Hmcn2	UTSW	2	31,361,800 (GRCm38)	missense	probably benign	0.11
R6479:Hmcn2	UTSW	2	31,425,468 (GRCm38)	missense	probably damaging	0.99
R6502:Hmcn2	UTSW	2	31,382,478 (GRCm38)	missense	probably damaging	0.99
R6511:Hmcn2	UTSW	2	31,356,342 (GRCm38)	missense	possibly damaging	0.79
R6537:Hmcn2	UTSW	2	31,415,268 (GRCm38)	missense	probably benign	0.00
R6880:Hmcn2	UTSW	2	31,343,056 (GRCm38)	missense	probably damaging	1.00
R6924:Hmcn2	UTSW	2	31,350,505 (GRCm38)	splice site	probably null
R7057:Hmcn2	UTSW	2	31,422,649 (GRCm38)	missense	probably damaging	0.99
R7141:Hmcn2	UTSW	2	31,360,896 (GRCm38)	missense	probably benign	0.17
R7268:Hmcn2	UTSW	2	31,457,966 (GRCm38)	missense	possibly damaging	0.48
R7307:Hmcn2	UTSW	2	31,343,081 (GRCm38)	missense	probably damaging	0.96
R7322:Hmcn2	UTSW	2	31,459,081 (GRCm38)	missense	probably damaging	0.99
R7334:Hmcn2	UTSW	2	31,453,135 (GRCm38)	missense	possibly damaging	0.82
R7334:Hmcn2	UTSW	2	31,435,794 (GRCm38)	missense	probably damaging	0.98
R7335:Hmcn2	UTSW	2	31,392,157 (GRCm38)	missense	possibly damaging	0.88
R7358:Hmcn2	UTSW	2	31,416,812 (GRCm38)	missense	probably damaging	1.00
R7359:Hmcn2	UTSW	2	31,388,383 (GRCm38)	missense	probably benign	0.13
R7488:Hmcn2	UTSW	2	31,420,830 (GRCm38)	missense	probably damaging	1.00
R7498:Hmcn2	UTSW	2	31,383,475 (GRCm38)	splice site	probably null
R7560:Hmcn2	UTSW	2	31,457,173 (GRCm38)	missense	probably benign
R7566:Hmcn2	UTSW	2	31,454,857 (GRCm38)	missense	probably damaging	0.96
R7570:Hmcn2	UTSW	2	31,423,911 (GRCm38)	missense	probably benign
R7574:Hmcn2	UTSW	2	31,455,519 (GRCm38)	missense	possibly damaging	0.68
R7599:Hmcn2	UTSW	2	31,356,286 (GRCm38)	missense	possibly damaging	0.93
R7654:Hmcn2	UTSW	2	31,346,569 (GRCm38)	missense	probably benign	0.00
R7662:Hmcn2	UTSW	2	31,382,345 (GRCm38)	missense	probably benign	0.01
R7666:Hmcn2	UTSW	2	31,380,233 (GRCm38)	missense	probably damaging	1.00
R7698:Hmcn2	UTSW	2	31,423,153 (GRCm38)	missense	probably damaging	0.98
R7722:Hmcn2	UTSW	2	31,382,500 (GRCm38)	nonsense	probably null
R7739:Hmcn2	UTSW	2	31,458,026 (GRCm38)	missense	possibly damaging	0.48
R7749:Hmcn2	UTSW	2	31,453,033 (GRCm38)	splice site	probably null
R7828:Hmcn2	UTSW	2	31,405,875 (GRCm38)	missense	possibly damaging	0.95
R7912:Hmcn2	UTSW	2	31,420,299 (GRCm38)	missense	probably benign	0.00
R7978:Hmcn2	UTSW	2	31,389,347 (GRCm38)	missense	probably benign	0.40
R8075:Hmcn2	UTSW	2	31,389,391 (GRCm38)	missense	possibly damaging	0.88
R8088:Hmcn2	UTSW	2	31,426,903 (GRCm38)	nonsense	probably null
R8101:Hmcn2	UTSW	2	31,350,070 (GRCm38)	missense	probably benign	0.08
R8124:Hmcn2	UTSW	2	31,400,124 (GRCm38)	missense	probably benign	0.01
R8145:Hmcn2	UTSW	2	31,423,105 (GRCm38)	missense	probably damaging	1.00
R8230:Hmcn2	UTSW	2	31,344,473 (GRCm38)	missense	possibly damaging	0.91
R8267:Hmcn2	UTSW	2	31,459,179 (GRCm38)	missense	probably benign
R8277:Hmcn2	UTSW	2	31,369,177 (GRCm38)	missense	probably benign	0.16
R8307:Hmcn2	UTSW	2	31,396,115 (GRCm38)	missense	probably damaging	0.99
R8353:Hmcn2	UTSW	2	31,385,341 (GRCm38)	splice site	probably null
R8415:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8416:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8437:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8438:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8440:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8442:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8497:Hmcn2	UTSW	2	31,423,345 (GRCm38)	missense	possibly damaging	0.92
R8520:Hmcn2	UTSW	2	31,354,714 (GRCm38)	missense	probably damaging	1.00
R8530:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8537:Hmcn2	UTSW	2	31,391,076 (GRCm38)	missense	probably benign	0.15
R8550:Hmcn2	UTSW	2	31,350,642 (GRCm38)	critical splice donor site	probably null
R8721:Hmcn2	UTSW	2	31,425,177 (GRCm38)	missense	probably damaging	1.00
R8795:Hmcn2	UTSW	2	31,425,381 (GRCm38)	missense	probably benign	0.01
R8802:Hmcn2	UTSW	2	31,411,276 (GRCm38)	missense	probably damaging	0.97
R8804:Hmcn2	UTSW	2	31,425,381 (GRCm38)	missense	probably benign	0.01
R8805:Hmcn2	UTSW	2	31,425,381 (GRCm38)	missense	probably benign	0.01
R8904:Hmcn2	UTSW	2	31,433,392 (GRCm38)	missense	possibly damaging	0.92
R8937:Hmcn2	UTSW	2	31,314,415 (GRCm38)	start codon destroyed	probably benign	0.01
R8947:Hmcn2	UTSW	2	31,388,208 (GRCm38)	missense	probably damaging	0.99
R8948:Hmcn2	UTSW	2	31,354,729 (GRCm38)	missense	probably damaging	1.00
R8950:Hmcn2	UTSW	2	31,354,729 (GRCm38)	missense	probably damaging	1.00
R8959:Hmcn2	UTSW	2	31,392,147 (GRCm38)	missense	probably damaging	1.00
R9025:Hmcn2	UTSW	2	31,457,955 (GRCm38)	missense	possibly damaging	0.56
R9039:Hmcn2	UTSW	2	31,354,634 (GRCm38)	missense	probably damaging	0.97
R9068:Hmcn2	UTSW	2	31,413,673 (GRCm38)	missense	probably benign	0.01
R9161:Hmcn2	UTSW	2	31,352,746 (GRCm38)	missense	probably benign	0.02
R9178:Hmcn2	UTSW	2	31,391,509 (GRCm38)	missense	possibly damaging	0.77
R9204:Hmcn2	UTSW	2	31,388,365 (GRCm38)	missense	probably damaging	0.98
R9317:Hmcn2	UTSW	2	31,460,316 (GRCm38)	missense	possibly damaging	0.91
R9341:Hmcn2	UTSW	2	31,389,347 (GRCm38)	missense	probably benign	0.40
R9343:Hmcn2	UTSW	2	31,389,347 (GRCm38)	missense	probably benign	0.40
R9355:Hmcn2	UTSW	2	31,438,290 (GRCm38)	missense	probably benign	0.18
R9371:Hmcn2	UTSW	2	31,411,905 (GRCm38)	missense	probably damaging	1.00
R9450:Hmcn2	UTSW	2	31,426,833 (GRCm38)	missense	probably damaging	1.00
R9477:Hmcn2	UTSW	2	31,396,019 (GRCm38)	critical splice acceptor site	probably null
R9483:Hmcn2	UTSW	2	31,430,363 (GRCm38)	missense
R9536:Hmcn2	UTSW	2	31,445,118 (GRCm38)	missense	possibly damaging	0.86
R9580:Hmcn2	UTSW	2	31,404,863 (GRCm38)	missense	probably benign	0.16
R9593:Hmcn2	UTSW	2	31,354,730 (GRCm38)	missense	probably damaging	0.99
R9649:Hmcn2	UTSW	2	31,402,438 (GRCm38)	missense	possibly damaging	0.95
R9706:Hmcn2	UTSW	2	31,415,267 (GRCm38)	missense	probably benign	0.00
X0066:Hmcn2	UTSW	2	31,454,811 (GRCm38)	missense	possibly damaging	0.83
X0067:Hmcn2	UTSW	2	31,405,867 (GRCm38)	missense	possibly damaging	0.82
Z1088:Hmcn2	UTSW	2	31,459,064 (GRCm38)	splice site	probably null
Z1088:Hmcn2	UTSW	2	31,381,067 (GRCm38)	missense	probably benign	0.01
Z1176:Hmcn2	UTSW	2	31,429,091 (GRCm38)	missense	probably damaging	0.97
Z1176:Hmcn2	UTSW	2	31,425,416 (GRCm38)	missense	probably damaging	1.00
Z1176:Hmcn2	UTSW	2	31,344,029 (GRCm38)	missense	possibly damaging	0.95
Z1177:Hmcn2	UTSW	2	31,426,824 (GRCm38)	missense	probably damaging	0.99
Z1177:Hmcn2	UTSW	2	31,344,506 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTAAGGGCTGAGTGCTAACCAG -3'
(R):5'- ATATCCAGTTGCCTCGGAAC -3'

Sequencing Primer
(F):5'- CTGTGATTGAGAATGGCCTCCC -3'
(R):5'- AGTTGCCTCGGAACCTTGG -3'

Posted On

2018-11-28