Mutagenetix > Incidental Mutation

Incidental Mutation 'R6981:Or5g27'

542653

Institutional Source

Beutler Lab

Gene Symbol

Or5g27

Ensembl Gene

ENSMUSG00000111179

Gene Name

olfactory receptor family 5 subfamily G member 27

Synonyms

MOR175-4, GA_x6K02T2Q125-47058060-47059004, Olfr996

MMRRC Submission

045089-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.118) question?

Stock #

R6981 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85409559-85410626 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85409825 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 81 (M81V)

Ref Sequence

ENSEMBL: ENSMUSP00000076330 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077075]

AlphaFold

Q7TRA0

Predicted Effect

probably benign
Transcript: ENSMUST00000077075
AA Change: M81V

PolyPhen 2 Score 0.059 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000076330
Gene: ENSMUSG00000111179
AA Change: M81V

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.2e-52	PFAM
Pfam:7tm_1	41	290	2.7e-16	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.4%
20x: 97.7%

Validation Efficiency

99% (68/69)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930438A08Rik	G	A	11: 58,184,544 (GRCm39)		probably benign	Het
5031439G07Rik	A	C	15: 84,833,798 (GRCm39)	Y419*	probably null	Het
Abca2	T	A	2: 25,334,151 (GRCm39)	F1809L	probably damaging	Het
Ache	C	T	5: 137,289,940 (GRCm39)	T423I	probably benign	Het
Acvrl1	A	G	15: 101,036,226 (GRCm39)	T395A	probably damaging	Het
Ap3b2	A	G	7: 81,127,741 (GRCm39)	I145T	probably damaging	Het
Arhgef4	A	C	1: 34,761,533 (GRCm39)	Q263P	unknown	Het
Asgr2	C	T	11: 69,987,636 (GRCm39)	L45F	probably damaging	Het
Baiap2l1	T	A	5: 144,222,389 (GRCm39)	Y122F	possibly damaging	Het
C1ra	G	A	6: 124,494,684 (GRCm39)	E316K	probably benign	Het
Car8	A	T	4: 8,185,650 (GRCm39)		probably null	Het
Carns1	T	C	19: 4,220,081 (GRCm39)	T385A	probably benign	Het
Ccdc47	T	C	11: 106,093,563 (GRCm39)	T41A	probably benign	Het
Ccne1	A	T	7: 37,797,998 (GRCm39)		probably benign	Het
Cdh4	C	T	2: 179,439,297 (GRCm39)	T148I	probably benign	Het
Cep85	C	T	4: 133,879,572 (GRCm39)	R392Q	probably damaging	Het
Ces1h	T	C	8: 94,080,123 (GRCm39)	T464A	unknown	Het
Cfl1	T	A	19: 5,542,644 (GRCm39)	S41R	possibly damaging	Het
Crnn	T	C	3: 93,055,442 (GRCm39)	V76A	probably damaging	Het
Cspg4	A	G	9: 56,794,385 (GRCm39)	T707A	probably benign	Het
Dgkh	A	T	14: 78,865,182 (GRCm39)	C53*	probably null	Het
Dhx34	G	T	7: 15,949,255 (GRCm39)	A391E	possibly damaging	Het
Dlx1	C	A	2: 71,362,697 (GRCm39)	N201K	probably benign	Het
Dnah6	T	C	6: 72,998,161 (GRCm39)	E4087G	probably benign	Het
Dock6	T	C	9: 21,756,846 (GRCm39)	Y134C	probably damaging	Het
Duox2	A	G	2: 122,121,708 (GRCm39)	V662A	possibly damaging	Het
Dusp12	A	G	1: 170,708,530 (GRCm39)	F12L	probably damaging	Het
Eppk1	T	C	15: 75,995,237 (GRCm39)	E548G	probably benign	Het
Foxj2	A	G	6: 122,819,798 (GRCm39)	D562G	probably benign	Het
Foxj2	A	G	6: 122,805,403 (GRCm39)	I92V	probably damaging	Het
Gm17728	A	G	17: 9,640,991 (GRCm39)	R34G	probably damaging	Het
Gpc6	T	C	14: 117,861,960 (GRCm39)	I292T	probably damaging	Het
Gpr15	T	A	16: 58,538,548 (GRCm39)	K180N	probably benign	Het
Gtf2ird1	G	T	5: 134,412,776 (GRCm39)		probably benign	Het
H2ac12	A	G	13: 22,219,719 (GRCm39)	S2P	probably benign	Het
Hps3	T	A	3: 20,076,984 (GRCm39)	T393S	probably damaging	Het
Hspa1a	A	T	17: 35,189,267 (GRCm39)		probably null	Het
Hydin	A	G	8: 111,257,704 (GRCm39)	E2378G	possibly damaging	Het
Ighv1-18	A	G	12: 114,646,298 (GRCm39)	L102P	probably damaging	Het
Itga5	T	A	15: 103,258,653 (GRCm39)	N814I	probably benign	Het
Kcnb2	T	C	1: 15,780,480 (GRCm39)	S451P	probably damaging	Het
Klhl32	T	G	4: 24,709,030 (GRCm39)	I112L	probably damaging	Het
Knstrn	T	G	2: 118,664,575 (GRCm39)	I47R	possibly damaging	Het
Med23	T	A	10: 24,771,722 (GRCm39)	S581T	possibly damaging	Het
Mgat5	C	T	1: 127,318,588 (GRCm39)	T361I	probably damaging	Het
Nipal3	A	G	4: 135,206,858 (GRCm39)	V112A	probably damaging	Het
Or10a5	T	A	7: 106,635,956 (GRCm39)	V198D	possibly damaging	Het
Or2b2	A	G	13: 21,887,243 (GRCm39)	E24G	probably benign	Het
Or4k41	T	C	2: 111,279,697 (GRCm39)	F71L	probably benign	Het
Paxip1	G	A	5: 27,970,766 (GRCm39)	Q528*	probably null	Het
Proser2	T	C	2: 6,118,801 (GRCm39)	D14G	probably damaging	Het
Rp1	T	G	1: 4,415,878 (GRCm39)	I1745L	probably benign	Het
Rxfp2	G	T	5: 149,972,313 (GRCm39)		probably null	Het
Slc45a1	T	C	4: 150,723,051 (GRCm39)	S278G	possibly damaging	Het
Smurf1	A	G	5: 144,823,179 (GRCm39)	I455T	possibly damaging	Het
Speg	T	C	1: 75,407,557 (GRCm39)	L3188P	probably damaging	Het
Tcaf3	G	T	6: 42,574,059 (GRCm39)	A51D	probably damaging	Het
Tecrl	T	C	5: 83,502,768 (GRCm39)	N12S	possibly damaging	Het
Tmem17	T	A	11: 22,468,508 (GRCm39)	I149N	possibly damaging	Het
Tmem171	A	G	13: 98,828,976 (GRCm39)	V58A	possibly damaging	Het
Ttn	A	G	2: 76,691,521 (GRCm39)		probably benign	Het
Ubqln5	A	G	7: 103,777,808 (GRCm39)	S339P	probably benign	Het
Vmn1r16	T	A	6: 57,300,473 (GRCm39)	I50L	probably benign	Het
Vmn2r103	A	T	17: 20,013,739 (GRCm39)	Y177F	probably benign	Het
Zfp28	C	A	7: 6,397,692 (GRCm39)	T709K	probably damaging	Het
Zfp958	A	G	8: 4,676,170 (GRCm39)	N46S	probably benign	Het
Zyx	T	A	6: 42,327,291 (GRCm39)	V30E	unknown	Het

Other mutations in Or5g27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01550:Or5g27	APN	2	85,409,875 (GRCm39)	nonsense	probably null
IGL01972:Or5g27	APN	2	85,410,117 (GRCm39)	missense	probably damaging	1.00
IGL02102:Or5g27	APN	2	85,410,017 (GRCm39)	missense	probably damaging	0.98
IGL03159:Or5g27	APN	2	85,410,284 (GRCm39)	missense	probably damaging	0.99
R0539:Or5g27	UTSW	2	85,410,119 (GRCm39)	missense	probably damaging	0.99
R4275:Or5g27	UTSW	2	85,410,207 (GRCm39)	missense	probably benign	0.07
R4561:Or5g27	UTSW	2	85,409,964 (GRCm39)	missense	probably damaging	0.99
R4953:Or5g27	UTSW	2	85,410,069 (GRCm39)	nonsense	probably null
R5794:Or5g27	UTSW	2	85,409,685 (GRCm39)	missense	probably benign	0.03
R6061:Or5g27	UTSW	2	85,409,886 (GRCm39)	missense	possibly damaging	0.48
R6695:Or5g27	UTSW	2	85,409,793 (GRCm39)	missense	probably damaging	1.00
R7030:Or5g27	UTSW	2	85,409,746 (GRCm39)	missense	possibly damaging	0.56
R7399:Or5g27	UTSW	2	85,409,640 (GRCm39)	missense	probably benign	0.00
R8914:Or5g27	UTSW	2	85,410,056 (GRCm39)	missense	possibly damaging	0.72
R9594:Or5g27	UTSW	2	85,409,882 (GRCm39)	nonsense	probably null
R9686:Or5g27	UTSW	2	85,410,340 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATCAGACTGTCATGCCTGAG -3'
(R):5'- TGGAGACATAATAAGTGTGTACAGC -3'

Sequencing Primer
(F):5'- AGACTGTCATGCCTGAGTTTCTC -3'
(R):5'- CAAGGGCTTACATATGGCTGCATAC -3'

Posted On

2018-11-28