Mutagenetix > Incidental Mutation

Incidental Mutation 'R6988:Gm436'

543074

Institutional Source

Beutler Lab

Gene Symbol

Gm436

Ensembl Gene

ENSMUSG00000078505

Gene Name

predicted gene 436

Synonyms

LOC230890

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.049) question?

Stock #

R6988 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

144669937-144686368 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 144686325 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 15 (F15S)

Ref Sequence

ENSEMBL: ENSMUSP00000101373 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105747]

AlphaFold

B1AVU6

Predicted Effect

probably benign
Transcript: ENSMUST00000105747
AA Change: F15S

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000101373
Gene: ENSMUSG00000078505
AA Change: F15S

Domain	Start	End	E-Value	Type
transmembrane domain	2	24	N/A	INTRINSIC
Pfam:Abhydrolase_3	115	285	1.6e-29	PFAM
Pfam:Abhydrolase_3	292	381	9e-12	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.2%
20x: 97.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700013G24Rik	T	G	4: 137,454,579 (GRCm38)	L15W	probably damaging	Het
4930550C14Rik	G	A	9: 53,411,756 (GRCm38)	V31I	possibly damaging	Het
4932415D10Rik	T	C	10: 82,291,899 (GRCm38)	D1759G	possibly damaging	Het
Adgre1	C	T	17: 57,408,445 (GRCm38)	S255F	probably benign	Het
Aff4	T	G	11: 53,398,237 (GRCm38)	S404R	probably damaging	Het
Akr1c19	A	T	13: 4,233,758 (GRCm38)		probably benign	Het
Ankrd31	T	A	13: 96,878,249 (GRCm38)	I1342K	probably damaging	Het
Arhgap5	T	A	12: 52,518,125 (GRCm38)	D626E	possibly damaging	Het
Arhgef1	G	A	7: 24,916,923 (GRCm38)	V332I	probably benign	Het
AY358078	A	G	14: 51,826,187 (GRCm38)	E430G	probably damaging	Het
B4gat1	T	A	19: 5,040,434 (GRCm38)	I395N	probably benign	Het
Bub1b	T	A	2: 118,636,830 (GRCm38)	I878N	probably damaging	Het
Ccdc150	C	T	1: 54,355,709 (GRCm38)	Q745*	probably null	Het
Ccl19	A	T	4: 42,754,885 (GRCm38)	I87N	probably damaging	Het
Ces2g	G	C	8: 104,963,908 (GRCm38)	G107A	probably benign	Het
Chpt1	A	G	10: 88,488,406 (GRCm38)	V180A	probably damaging	Het
Col2a1	T	C	15: 98,004,454 (GRCm38)	T14A	unknown	Het
Dnah7a	T	C	1: 53,582,625 (GRCm38)	I1114V	possibly damaging	Het
Dnah7c	T	C	1: 46,666,213 (GRCm38)	I2462T	possibly damaging	Het
Dnah8	T	C	17: 30,643,275 (GRCm38)	F208S	probably damaging	Het
Dnhd1	A	G	7: 105,714,210 (GRCm38)	E3993G	probably damaging	Het
Erv3	C	T	2: 131,855,966 (GRCm38)	D158N	possibly damaging	Het
Exoc6	T	A	19: 37,609,091 (GRCm38)	F647I	probably damaging	Het
Fbrs	G	A	7: 127,479,508 (GRCm38)		probably benign	Het
Fgfr1op2	T	C	6: 146,589,965 (GRCm38)	F109L	probably damaging	Het
Fv1	T	C	4: 147,869,271 (GRCm38)	F98S	possibly damaging	Het
H2-M10.1	T	C	17: 36,325,592 (GRCm38)	K107E	probably benign	Het
Hspg2	A	T	4: 137,528,890 (GRCm38)	Q1436L	probably damaging	Het
Ighv1-74	T	C	12: 115,802,763 (GRCm38)	Y79C	probably damaging	Het
Kcnj1	G	A	9: 32,396,585 (GRCm38)	V102I	probably benign	Het
Mnt	C	A	11: 74,842,809 (GRCm38)		probably benign	Het
Mrpl15	T	C	1: 4,782,660 (GRCm38)	T112A	probably benign	Het
Ncdn	T	C	4: 126,747,189 (GRCm38)	D506G	probably benign	Het
Ogdh	C	T	11: 6,313,806 (GRCm38)	R81*	probably null	Het
Olfr791	T	A	10: 129,526,673 (GRCm38)	S149T	probably benign	Het
Olfr815	G	C	10: 129,902,409 (GRCm38)	F100L	probably damaging	Het
Pole	G	T	5: 110,329,583 (GRCm38)	V1863F	probably damaging	Het
Pramel5	T	C	4: 144,274,007 (GRCm38)		probably benign	Het
Rabep1	A	G	11: 70,934,537 (GRCm38)	K636E	probably damaging	Het
Rasgrf2	T	A	13: 91,885,635 (GRCm38)	Y1151F	probably benign	Het
Rrad	A	G	8: 104,630,636 (GRCm38)	V93A	probably damaging	Het
Sesn3	A	T	9: 14,310,257 (GRCm38)	R118*	probably null	Het
Slc27a3	T	C	3: 90,386,290 (GRCm38)	N596S	probably benign	Het
Snx19	C	A	9: 30,428,935 (GRCm38)	D456E	probably damaging	Het
Supt20	T	C	3: 54,698,597 (GRCm38)	S35P	probably damaging	Het
Syde2	G	T	3: 146,019,809 (GRCm38)	R885L	probably benign	Het
Synm	G	A	7: 67,733,658 (GRCm38)	L1419F	probably damaging	Het
Tcrg-V6	G	T	13: 19,190,644 (GRCm38)	G40W	possibly damaging	Het
Tekt2	A	G	4: 126,323,443 (GRCm38)	F221L	probably benign	Het
Ticam1	C	T	17: 56,269,900 (GRCm38)	E732K	probably benign	Het
Tmem39b	A	G	4: 129,693,148 (GRCm38)	I90T	possibly damaging	Het
Trib2	A	G	12: 15,815,338 (GRCm38)	S79P	probably damaging	Het
Usp32	T	C	11: 85,010,143 (GRCm38)	M1084V	probably benign	Het
Vmn1r181	T	C	7: 23,984,847 (GRCm38)	F246L	probably damaging	Het
Wnt16	A	G	6: 22,288,511 (GRCm38)	D2G	probably damaging	Het
Zfp462	A	G	4: 55,080,716 (GRCm38)	E1357G	probably benign	Het
Zhx3	A	G	2: 160,779,868 (GRCm38)	M793T	probably benign	Het

Other mutations in Gm436

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01149:Gm436	APN	4	144,673,779 (GRCm38)	missense	probably benign	0.12
IGL01369:Gm436	APN	4	144,674,645 (GRCm38)	missense	possibly damaging	0.50
IGL01503:Gm436	APN	4	144,674,567 (GRCm38)	missense	probably damaging	0.99
IGL01505:Gm436	APN	4	144,674,618 (GRCm38)	missense	probably damaging	1.00
IGL01954:Gm436	APN	4	144,670,171 (GRCm38)	missense	probably damaging	1.00
IGL02447:Gm436	APN	4	144,674,699 (GRCm38)	missense	probably benign	0.02
IGL02804:Gm436	APN	4	144,670,439 (GRCm38)	missense	possibly damaging	0.95
R0373:Gm436	UTSW	4	144,686,220 (GRCm38)	missense	possibly damaging	0.54
R1124:Gm436	UTSW	4	144,670,275 (GRCm38)	missense	probably benign	0.00
R1598:Gm436	UTSW	4	144,670,424 (GRCm38)	missense	possibly damaging	0.60
R1734:Gm436	UTSW	4	144,670,026 (GRCm38)	missense	probably benign	0.04
R1763:Gm436	UTSW	4	144,669,959 (GRCm38)	missense	probably benign	0.24
R1968:Gm436	UTSW	4	144,670,623 (GRCm38)	missense	possibly damaging	0.83
R3055:Gm436	UTSW	4	144,674,698 (GRCm38)	missense	probably benign	0.16
R3056:Gm436	UTSW	4	144,674,698 (GRCm38)	missense	probably benign	0.16
R3930:Gm436	UTSW	4	144,670,128 (GRCm38)	missense	probably damaging	1.00
R5124:Gm436	UTSW	4	144,674,719 (GRCm38)	missense	probably damaging	1.00
R5407:Gm436	UTSW	4	144,670,325 (GRCm38)	missense	probably benign	0.01
R6302:Gm436	UTSW	4	144,670,190 (GRCm38)	nonsense	probably null
R6814:Gm436	UTSW	4	144,670,646 (GRCm38)	nonsense	probably null
R6872:Gm436	UTSW	4	144,670,646 (GRCm38)	nonsense	probably null
R7131:Gm436	UTSW	4	144,670,067 (GRCm38)	missense	probably damaging	0.98
R7895:Gm436	UTSW	4	144,670,343 (GRCm38)	missense	possibly damaging	0.95
R8108:Gm436	UTSW	4	144,670,669 (GRCm38)	missense	probably benign	0.04
R8378:Gm436	UTSW	4	144,670,599 (GRCm38)	missense	probably benign	0.24
R8676:Gm436	UTSW	4	144,670,113 (GRCm38)	missense	possibly damaging	0.94
R8817:Gm436	UTSW	4	144,673,791 (GRCm38)	missense	probably benign	0.00
R9328:Gm436	UTSW	4	144,674,686 (GRCm38)	missense	probably benign	0.02
R9640:Gm436	UTSW	4	144,686,239 (GRCm38)	missense	possibly damaging	0.66

Predicted Primers

PCR Primer
(F):5'- CCTCAGACTTCATTTTGTGGGAATC -3'
(R):5'- TTCAGACAAGAAGCACGTGTG -3'

Sequencing Primer
(F):5'- AGACTTCATTTTGTGGGAATCTAGAG -3'
(R):5'- CACGTGTGAGGCAATGAGC -3'

Posted On

2018-11-28