Mutagenetix > Incidental Mutation

Incidental Mutation 'R6988:Usp32'

543098

Institutional Source

Beutler Lab

Gene Symbol

Usp32

Ensembl Gene

ENSMUSG00000000804

Gene Name

ubiquitin specific peptidase 32

Synonyms

6430526O11Rik, 2900074J03Rik

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6988 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

84984442-85140161 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85010143 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 1084 (M1084V)

Ref Sequence

ENSEMBL: ENSMUSP00000103710 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108075]

AlphaFold

F8VPZ3

Predicted Effect

probably benign
Transcript: ENSMUST00000000821

SMART Domains

Protein: ENSMUSP00000000821
Gene: ENSMUSG00000000804

Domain	Start	End	E-Value	Type
Pfam:UCH	32	260	4.1e-51	PFAM
Pfam:UCH_1	33	228	1.7e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000108075
AA Change: M1084V

PolyPhen 2 Score 0.348 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000103710
Gene: ENSMUSG00000000804
AA Change: M1084V

Domain	Start	End	E-Value	Type
EFh	232	260	4.66e0	SMART
EFh	268	296	5.8e-1	SMART
Blast:EFh	318	346	5e-7	BLAST
DUSP	389	588	2.32e-16	SMART
Pfam:Ubiquitin_3	628	711	2.4e-9	PFAM
Pfam:UCH	733	1564	2.4e-83	PFAM
Pfam:UCH_1	1202	1547	2.9e-12	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.2%
20x: 97.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700013G24Rik	T	G	4: 137,454,579 (GRCm38)	L15W	probably damaging	Het
4930550C14Rik	G	A	9: 53,411,756 (GRCm38)	V31I	possibly damaging	Het
4932415D10Rik	T	C	10: 82,291,899 (GRCm38)	D1759G	possibly damaging	Het
Adgre1	C	T	17: 57,408,445 (GRCm38)	S255F	probably benign	Het
Aff4	T	G	11: 53,398,237 (GRCm38)	S404R	probably damaging	Het
Akr1c19	A	T	13: 4,233,758 (GRCm38)		probably benign	Het
Ankrd31	T	A	13: 96,878,249 (GRCm38)	I1342K	probably damaging	Het
Arhgap5	T	A	12: 52,518,125 (GRCm38)	D626E	possibly damaging	Het
Arhgef1	G	A	7: 24,916,923 (GRCm38)	V332I	probably benign	Het
AY358078	A	G	14: 51,826,187 (GRCm38)	E430G	probably damaging	Het
B4gat1	T	A	19: 5,040,434 (GRCm38)	I395N	probably benign	Het
Bub1b	T	A	2: 118,636,830 (GRCm38)	I878N	probably damaging	Het
Ccdc150	C	T	1: 54,355,709 (GRCm38)	Q745*	probably null	Het
Ccl19	A	T	4: 42,754,885 (GRCm38)	I87N	probably damaging	Het
Ces2g	G	C	8: 104,963,908 (GRCm38)	G107A	probably benign	Het
Chpt1	A	G	10: 88,488,406 (GRCm38)	V180A	probably damaging	Het
Col2a1	T	C	15: 98,004,454 (GRCm38)	T14A	unknown	Het
Dnah7a	T	C	1: 53,582,625 (GRCm38)	I1114V	possibly damaging	Het
Dnah7c	T	C	1: 46,666,213 (GRCm38)	I2462T	possibly damaging	Het
Dnah8	T	C	17: 30,643,275 (GRCm38)	F208S	probably damaging	Het
Dnhd1	A	G	7: 105,714,210 (GRCm38)	E3993G	probably damaging	Het
Erv3	C	T	2: 131,855,966 (GRCm38)	D158N	possibly damaging	Het
Exoc6	T	A	19: 37,609,091 (GRCm38)	F647I	probably damaging	Het
Fbrs	G	A	7: 127,479,508 (GRCm38)		probably benign	Het
Fgfr1op2	T	C	6: 146,589,965 (GRCm38)	F109L	probably damaging	Het
Fv1	T	C	4: 147,869,271 (GRCm38)	F98S	possibly damaging	Het
Gm436	A	G	4: 144,686,325 (GRCm38)	F15S	probably benign	Het
H2-M10.1	T	C	17: 36,325,592 (GRCm38)	K107E	probably benign	Het
Hspg2	A	T	4: 137,528,890 (GRCm38)	Q1436L	probably damaging	Het
Ighv1-74	T	C	12: 115,802,763 (GRCm38)	Y79C	probably damaging	Het
Kcnj1	G	A	9: 32,396,585 (GRCm38)	V102I	probably benign	Het
Mnt	C	A	11: 74,842,809 (GRCm38)		probably benign	Het
Mrpl15	T	C	1: 4,782,660 (GRCm38)	T112A	probably benign	Het
Ncdn	T	C	4: 126,747,189 (GRCm38)	D506G	probably benign	Het
Ogdh	C	T	11: 6,313,806 (GRCm38)	R81*	probably null	Het
Olfr791	T	A	10: 129,526,673 (GRCm38)	S149T	probably benign	Het
Olfr815	G	C	10: 129,902,409 (GRCm38)	F100L	probably damaging	Het
Pole	G	T	5: 110,329,583 (GRCm38)	V1863F	probably damaging	Het
Pramel5	T	C	4: 144,274,007 (GRCm38)		probably benign	Het
Rabep1	A	G	11: 70,934,537 (GRCm38)	K636E	probably damaging	Het
Rasgrf2	T	A	13: 91,885,635 (GRCm38)	Y1151F	probably benign	Het
Rrad	A	G	8: 104,630,636 (GRCm38)	V93A	probably damaging	Het
Sesn3	A	T	9: 14,310,257 (GRCm38)	R118*	probably null	Het
Slc27a3	T	C	3: 90,386,290 (GRCm38)	N596S	probably benign	Het
Snx19	C	A	9: 30,428,935 (GRCm38)	D456E	probably damaging	Het
Supt20	T	C	3: 54,698,597 (GRCm38)	S35P	probably damaging	Het
Syde2	G	T	3: 146,019,809 (GRCm38)	R885L	probably benign	Het
Synm	G	A	7: 67,733,658 (GRCm38)	L1419F	probably damaging	Het
Tcrg-V6	G	T	13: 19,190,644 (GRCm38)	G40W	possibly damaging	Het
Tekt2	A	G	4: 126,323,443 (GRCm38)	F221L	probably benign	Het
Ticam1	C	T	17: 56,269,900 (GRCm38)	E732K	probably benign	Het
Tmem39b	A	G	4: 129,693,148 (GRCm38)	I90T	possibly damaging	Het
Trib2	A	G	12: 15,815,338 (GRCm38)	S79P	probably damaging	Het
Vmn1r181	T	C	7: 23,984,847 (GRCm38)	F246L	probably damaging	Het
Wnt16	A	G	6: 22,288,511 (GRCm38)	D2G	probably damaging	Het
Zfp462	A	G	4: 55,080,716 (GRCm38)	E1357G	probably benign	Het
Zhx3	A	G	2: 160,779,868 (GRCm38)	M793T	probably benign	Het

Other mutations in Usp32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00529:Usp32	APN	11	84,994,426 (GRCm38)	missense	probably damaging	1.00
IGL00701:Usp32	APN	11	85,059,125 (GRCm38)	splice site	probably null
IGL00848:Usp32	APN	11	85,051,181 (GRCm38)	splice site	probably benign
IGL00934:Usp32	APN	11	85,007,076 (GRCm38)	missense	probably damaging	1.00
IGL01019:Usp32	APN	11	85,039,265 (GRCm38)	missense	probably damaging	0.97
IGL01302:Usp32	APN	11	84,988,482 (GRCm38)	missense	probably benign	0.05
IGL01444:Usp32	APN	11	85,059,164 (GRCm38)	missense	probably damaging	0.97
IGL01575:Usp32	APN	11	85,022,802 (GRCm38)	missense	probably damaging	1.00
IGL01981:Usp32	APN	11	85,036,524 (GRCm38)	missense	probably benign	0.02
IGL02118:Usp32	APN	11	85,032,177 (GRCm38)	nonsense	probably null
IGL02159:Usp32	APN	11	85,005,802 (GRCm38)	splice site	probably null
IGL02227:Usp32	APN	11	84,986,481 (GRCm38)	missense	probably damaging	1.00
IGL02363:Usp32	APN	11	85,044,787 (GRCm38)	missense	probably benign	0.01
IGL02524:Usp32	APN	11	85,010,011 (GRCm38)	nonsense	probably null
IGL02613:Usp32	APN	11	85,040,070 (GRCm38)	missense	probably damaging	0.99
IGL02720:Usp32	APN	11	85,006,991 (GRCm38)	critical splice donor site	probably null
IGL02738:Usp32	APN	11	85,083,806 (GRCm38)	missense	probably damaging	1.00
IGL02929:Usp32	APN	11	84,988,372 (GRCm38)	missense	probably benign	0.01
IGL03303:Usp32	APN	11	85,022,832 (GRCm38)	missense	probably damaging	1.00
BB010:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
BB020:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
PIT4812001:Usp32	UTSW	11	85,010,074 (GRCm38)	missense	probably damaging	1.00
R0026:Usp32	UTSW	11	85,032,074 (GRCm38)	missense	possibly damaging	0.48
R0295:Usp32	UTSW	11	85,053,692 (GRCm38)	missense	probably damaging	0.98
R1320:Usp32	UTSW	11	85,017,793 (GRCm38)	missense	probably damaging	0.98
R1712:Usp32	UTSW	11	85,042,580 (GRCm38)	missense	probably benign	0.12
R1922:Usp32	UTSW	11	85,007,004 (GRCm38)	nonsense	probably null
R1973:Usp32	UTSW	11	85,103,931 (GRCm38)	missense	probably benign	0.09
R2010:Usp32	UTSW	11	85,040,004 (GRCm38)	missense	probably damaging	0.98
R2082:Usp32	UTSW	11	85,030,512 (GRCm38)	missense	probably damaging	0.99
R2355:Usp32	UTSW	11	85,005,909 (GRCm38)	missense	probably benign	0.34
R3147:Usp32	UTSW	11	85,029,087 (GRCm38)	missense	probably damaging	1.00
R3160:Usp32	UTSW	11	85,025,536 (GRCm38)	missense	probably damaging	0.97
R3162:Usp32	UTSW	11	85,025,536 (GRCm38)	missense	probably damaging	0.97
R3716:Usp32	UTSW	11	85,042,563 (GRCm38)	missense	probably damaging	1.00
R3816:Usp32	UTSW	11	84,994,384 (GRCm38)	critical splice donor site	probably null
R3870:Usp32	UTSW	11	85,007,055 (GRCm38)	nonsense	probably null
R3871:Usp32	UTSW	11	85,081,156 (GRCm38)	missense	probably null	0.81
R4041:Usp32	UTSW	11	85,017,739 (GRCm38)	missense	probably benign	0.40
R4079:Usp32	UTSW	11	85,039,229 (GRCm38)	missense	probably damaging	0.98
R4332:Usp32	UTSW	11	85,103,978 (GRCm38)	missense	possibly damaging	0.79
R4396:Usp32	UTSW	11	85,053,975 (GRCm38)	missense	probably benign
R4580:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably null
R4620:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably null
R4744:Usp32	UTSW	11	84,994,393 (GRCm38)	missense	probably damaging	1.00
R4909:Usp32	UTSW	11	85,055,772 (GRCm38)	nonsense	probably null
R5056:Usp32	UTSW	11	85,026,795 (GRCm38)	missense	probably benign	0.07
R5111:Usp32	UTSW	11	85,077,331 (GRCm38)	missense	possibly damaging	0.95
R5213:Usp32	UTSW	11	85,022,259 (GRCm38)	missense	probably damaging	1.00
R5308:Usp32	UTSW	11	85,017,718 (GRCm38)	missense	probably benign	0.12
R5381:Usp32	UTSW	11	85,059,127 (GRCm38)	critical splice donor site	probably benign
R5538:Usp32	UTSW	11	85,017,786 (GRCm38)	missense	possibly damaging	0.65
R5659:Usp32	UTSW	11	85,077,414 (GRCm38)	missense	possibly damaging	0.94
R6006:Usp32	UTSW	11	84,992,451 (GRCm38)	critical splice donor site	probably null
R6011:Usp32	UTSW	11	85,032,097 (GRCm38)	missense	possibly damaging	0.70
R6029:Usp32	UTSW	11	85,025,582 (GRCm38)	missense	probably damaging	0.99
R6074:Usp32	UTSW	11	84,994,573 (GRCm38)	missense	probably benign	0.00
R6331:Usp32	UTSW	11	84,986,576 (GRCm38)	missense	possibly damaging	0.92
R6353:Usp32	UTSW	11	85,022,281 (GRCm38)	missense	probably benign
R6714:Usp32	UTSW	11	85,026,870 (GRCm38)	missense	probably damaging	0.99
R6778:Usp32	UTSW	11	85,025,686 (GRCm38)	missense	probably benign	0.00
R6992:Usp32	UTSW	11	85,032,088 (GRCm38)	missense	probably damaging	0.99
R7182:Usp32	UTSW	11	85,040,170 (GRCm38)	missense	probably benign	0.34
R7186:Usp32	UTSW	11	85,051,234 (GRCm38)	missense	probably benign	0.45
R7198:Usp32	UTSW	11	85,022,855 (GRCm38)	frame shift	probably null
R7201:Usp32	UTSW	11	85,022,855 (GRCm38)	frame shift	probably null
R7469:Usp32	UTSW	11	84,988,553 (GRCm38)	missense	possibly damaging	0.94
R7502:Usp32	UTSW	11	85,022,898 (GRCm38)	missense	possibly damaging	0.48
R7513:Usp32	UTSW	11	85,027,112 (GRCm38)	nonsense	probably null
R7629:Usp32	UTSW	11	85,019,855 (GRCm38)	frame shift	probably null
R7703:Usp32	UTSW	11	85,077,327 (GRCm38)	missense	probably damaging	0.99
R7741:Usp32	UTSW	11	84,987,281 (GRCm38)	missense	probably damaging	0.99
R7765:Usp32	UTSW	11	84,994,408 (GRCm38)	missense	probably damaging	1.00
R7933:Usp32	UTSW	11	85,007,059 (GRCm38)	missense	probably damaging	1.00
R7973:Usp32	UTSW	11	85,022,808 (GRCm38)	missense	probably damaging	0.99
R7989:Usp32	UTSW	11	85,034,300 (GRCm38)	missense
R7998:Usp32	UTSW	11	84,994,426 (GRCm38)	missense	probably damaging	1.00
R8292:Usp32	UTSW	11	85,077,401 (GRCm38)	missense	probably damaging	0.99
R8305:Usp32	UTSW	11	85,032,185 (GRCm38)	missense	possibly damaging	0.83
R8548:Usp32	UTSW	11	85,017,827 (GRCm38)	missense	possibly damaging	0.52
R8924:Usp32	UTSW	11	85,025,544 (GRCm38)	missense	probably damaging	0.98
R9002:Usp32	UTSW	11	85,053,951 (GRCm38)	missense	probably damaging	0.96
R9145:Usp32	UTSW	11	85,022,292 (GRCm38)	missense	probably damaging	1.00
R9209:Usp32	UTSW	11	85,040,012 (GRCm38)	missense	probably damaging	0.98
R9211:Usp32	UTSW	11	85,022,733 (GRCm38)	missense	probably damaging	1.00
R9296:Usp32	UTSW	11	85,017,652 (GRCm38)	missense	probably damaging	1.00
R9310:Usp32	UTSW	11	85,051,202 (GRCm38)	missense	probably benign	0.29
R9417:Usp32	UTSW	11	84,994,543 (GRCm38)	missense	probably damaging	1.00
R9514:Usp32	UTSW	11	85,022,734 (GRCm38)	missense	probably damaging	0.99
R9652:Usp32	UTSW	11	85,030,491 (GRCm38)	missense	probably damaging	0.97
R9723:Usp32	UTSW	11	85,044,710 (GRCm38)	nonsense	probably null
R9757:Usp32	UTSW	11	85,077,329 (GRCm38)	nonsense	probably null
X0028:Usp32	UTSW	11	84,992,606 (GRCm38)	missense	probably benign	0.05
Z1177:Usp32	UTSW	11	84,988,612 (GRCm38)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AACCTACCAATCCTGTGCATG -3'
(R):5'- AAACCTTTCTGTGGGTCTCC -3'

Sequencing Primer
(F):5'- GCATGGTTGCTAGCTTCTTGAG -3'
(R):5'- TGGGTCTCCACTTTAAATGGTAG -3'

Posted On

2018-11-28