Mutagenetix > Incidental Mutation

Incidental Mutation 'R6990:Vmn2r2'

543185

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r2

Ensembl Gene

ENSMUSG00000043897

Gene Name

vomeronasal 2, receptor 2

Synonyms

MMRRC Submission

045096-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.093) question?

Stock #

R6990 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

64022699-64049349 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 64024608 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 658 (I658L)

Ref Sequence

ENSEMBL: ENSMUSP00000135110 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077958] [ENSMUST00000177151]

AlphaFold

L7N2E9

Predicted Effect

probably benign
Transcript: ENSMUST00000077958
AA Change: I574L

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000077109
Gene: ENSMUSG00000043897
AA Change: I574L

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	1	416	4.5e-80	PFAM
Pfam:NCD3G	458	511	8.3e-18	PFAM
Pfam:7tm_3	542	779	1.3e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000177151
AA Change: I658L

PolyPhen 2 Score 0.023 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000135110
Gene: ENSMUSG00000043897
AA Change: I658L

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	83	500	1.6e-84	PFAM
Pfam:NCD3G	542	595	2.6e-17	PFAM
Pfam:7tm_3	628	862	1.9e-47	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610008E11Rik	T	G	10: 78,902,925 (GRCm39)	T464P	probably damaging	Het
Abca16	A	G	7: 120,126,950 (GRCm39)	I1214V	probably benign	Het
Arap2	A	G	5: 62,833,860 (GRCm39)	F869S	probably damaging	Het
Astn2	G	T	4: 65,910,540 (GRCm39)	H431N	possibly damaging	Het
Bap1	A	G	14: 30,977,608 (GRCm39)	T308A	probably benign	Het
Bcr	G	A	10: 74,966,868 (GRCm39)	E492K	possibly damaging	Het
Bltp3b	A	G	10: 89,641,979 (GRCm39)	D1050G	probably benign	Het
Camta1	A	G	4: 151,229,501 (GRCm39)	F444L	probably damaging	Het
Chd7	A	T	4: 8,844,525 (GRCm39)	T1545S	probably benign	Het
Dnmt1	A	T	9: 20,827,110 (GRCm39)	Y877*	probably null	Het
Fam13b	A	G	18: 34,630,500 (GRCm39)	V86A	possibly damaging	Het
Gm11562	T	A	11: 99,510,817 (GRCm39)	R128W	unknown	Het
Krt86	A	G	15: 101,371,714 (GRCm39)	I95V	probably benign	Het
Mroh2b	A	G	15: 4,942,284 (GRCm39)	T349A	possibly damaging	Het
Ms4a7	A	T	19: 11,310,605 (GRCm39)	L38Q	probably damaging	Het
Myo1h	T	C	5: 114,468,221 (GRCm39)	S339P	probably damaging	Het
Nf2	T	C	11: 4,749,944 (GRCm39)	I46V	probably benign	Het
Nr1h4	T	C	10: 89,290,792 (GRCm39)	D416G	probably benign	Het
Oga	G	A	19: 45,755,915 (GRCm39)	A576V	probably benign	Het
Or11l3	T	C	11: 58,516,284 (GRCm39)	E196G	probably damaging	Het
Or4p18	A	G	2: 88,232,639 (GRCm39)	M213T	probably benign	Het
Pde1c	C	G	6: 56,419,020 (GRCm39)	E87Q	possibly damaging	Het
Peg10	T	TCCA	6: 4,756,451 (GRCm39)		probably benign	Het
Pga5	A	T	19: 10,646,779 (GRCm39)	D317E	probably benign	Het
Pira2	T	A	7: 3,844,067 (GRCm39)	K568N	probably damaging	Het
Ppp2cb	A	G	8: 34,109,161 (GRCm39)	D290G	probably benign	Het
Sall4	T	C	2: 168,596,990 (GRCm39)	K617E	probably damaging	Het
Slc7a14	G	A	3: 31,277,728 (GRCm39)	P626S	possibly damaging	Het
Speer4b	A	T	5: 27,702,076 (GRCm39)	L228*	probably null	Het
Spns2	T	C	11: 72,380,447 (GRCm39)	T59A	probably benign	Het
Tbc1d5	A	T	17: 51,275,260 (GRCm39)	N78K	probably benign	Het
Tmem63a	T	C	1: 180,788,686 (GRCm39)	V341A	probably benign	Het
Trpt1	A	G	19: 6,975,683 (GRCm39)	T146A	probably benign	Het
Tyrp1	T	C	4: 80,753,674 (GRCm39)	C122R	probably damaging	Het
Uchl1	A	G	5: 66,839,818 (GRCm39)	E120G	possibly damaging	Het
Ucn3	C	T	13: 3,991,295 (GRCm39)	R119Q	possibly damaging	Het
Vmn2r96	T	A	17: 18,804,082 (GRCm39)	V444E	probably benign	Het
Wdr54	A	C	6: 83,132,629 (GRCm39)		probably null	Het
Xcr1	A	G	9: 123,685,300 (GRCm39)	L154P	probably benign	Het
Zbtb26	T	C	2: 37,326,557 (GRCm39)	K160E	probably benign	Het
Zfp157	T	G	5: 138,454,772 (GRCm39)	Y323*	probably null	Het
Zfp850	A	T	7: 27,689,801 (GRCm39)	F136I	probably benign	Het

Other mutations in Vmn2r2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00326:Vmn2r2	APN	3	64,041,319 (GRCm39)	splice site	probably benign
IGL00980:Vmn2r2	APN	3	64,024,601 (GRCm39)	missense	probably benign	0.11
IGL01389:Vmn2r2	APN	3	64,024,430 (GRCm39)	missense	probably damaging	0.97
IGL01804:Vmn2r2	APN	3	64,041,677 (GRCm39)	missense	possibly damaging	0.91
IGL02750:Vmn2r2	APN	3	64,024,823 (GRCm39)	missense	probably damaging	1.00
IGL02829:Vmn2r2	APN	3	64,026,172 (GRCm39)	splice site	probably benign
IGL03036:Vmn2r2	APN	3	64,024,321 (GRCm39)	missense	probably benign	0.01
IGL03174:Vmn2r2	APN	3	64,024,544 (GRCm39)	nonsense	probably null
PIT4151001:Vmn2r2	UTSW	3	64,024,334 (GRCm39)	missense	possibly damaging	0.67
R0029:Vmn2r2	UTSW	3	64,024,365 (GRCm39)	missense	probably benign	0.00
R0310:Vmn2r2	UTSW	3	64,042,039 (GRCm39)	missense	probably damaging	1.00
R0357:Vmn2r2	UTSW	3	64,041,320 (GRCm39)	splice site	probably null
R0637:Vmn2r2	UTSW	3	64,033,999 (GRCm39)	missense	probably benign	0.00
R1626:Vmn2r2	UTSW	3	64,041,921 (GRCm39)	missense	possibly damaging	0.61
R1662:Vmn2r2	UTSW	3	64,024,551 (GRCm39)	missense	probably benign	0.00
R1710:Vmn2r2	UTSW	3	64,024,820 (GRCm39)	missense	probably benign	0.19
R1797:Vmn2r2	UTSW	3	64,042,128 (GRCm39)	missense	probably benign	0.00
R1862:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R1971:Vmn2r2	UTSW	3	64,034,121 (GRCm39)	missense	probably damaging	1.00
R2051:Vmn2r2	UTSW	3	64,024,766 (GRCm39)	missense	possibly damaging	0.77
R2099:Vmn2r2	UTSW	3	64,024,474 (GRCm39)	missense	probably damaging	1.00
R2275:Vmn2r2	UTSW	3	64,023,930 (GRCm39)	missense	probably benign	0.00
R3078:Vmn2r2	UTSW	3	64,042,053 (GRCm39)	missense	probably benign	0.22
R3418:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3419:Vmn2r2	UTSW	3	64,024,320 (GRCm39)	missense	probably benign	0.00
R3959:Vmn2r2	UTSW	3	64,047,947 (GRCm39)	missense	probably benign
R4230:Vmn2r2	UTSW	3	64,041,912 (GRCm39)	missense	probably benign	0.00
R4258:Vmn2r2	UTSW	3	64,042,118 (GRCm39)	missense	probably damaging	1.00
R4810:Vmn2r2	UTSW	3	64,044,883 (GRCm39)	missense	probably damaging	1.00
R4822:Vmn2r2	UTSW	3	64,041,960 (GRCm39)	missense	probably damaging	0.99
R4919:Vmn2r2	UTSW	3	64,024,578 (GRCm39)	missense	possibly damaging	0.56
R4925:Vmn2r2	UTSW	3	64,044,892 (GRCm39)	start codon destroyed	probably null	0.03
R4954:Vmn2r2	UTSW	3	64,047,905 (GRCm39)	missense	probably benign	0.00
R5071:Vmn2r2	UTSW	3	64,024,321 (GRCm39)	missense	probably benign	0.01
R5315:Vmn2r2	UTSW	3	64,024,377 (GRCm39)	missense	probably benign	0.37
R5450:Vmn2r2	UTSW	3	64,034,011 (GRCm39)	missense	probably benign
R5577:Vmn2r2	UTSW	3	64,024,416 (GRCm39)	missense	probably benign	0.00
R5595:Vmn2r2	UTSW	3	64,034,036 (GRCm39)	missense	possibly damaging	0.74
R5727:Vmn2r2	UTSW	3	64,024,608 (GRCm39)	missense	probably benign	0.41
R5810:Vmn2r2	UTSW	3	64,024,815 (GRCm39)	missense	probably damaging	1.00
R5919:Vmn2r2	UTSW	3	64,044,723 (GRCm39)	missense	probably benign	0.39
R6052:Vmn2r2	UTSW	3	64,024,782 (GRCm39)	missense	possibly damaging	0.56
R6084:Vmn2r2	UTSW	3	64,024,467 (GRCm39)	missense	probably benign	0.01
R6299:Vmn2r2	UTSW	3	64,024,074 (GRCm39)	nonsense	probably null
R6762:Vmn2r2	UTSW	3	64,041,870 (GRCm39)	missense	probably damaging	1.00
R6858:Vmn2r2	UTSW	3	64,044,915 (GRCm39)	missense	probably damaging	1.00
R6889:Vmn2r2	UTSW	3	64,024,688 (GRCm39)	missense	probably damaging	0.99
R7195:Vmn2r2	UTSW	3	64,023,900 (GRCm39)	missense	probably benign	0.01
R7269:Vmn2r2	UTSW	3	64,033,998 (GRCm39)	missense	probably benign	0.32
R7699:Vmn2r2	UTSW	3	64,024,536 (GRCm39)	missense	possibly damaging	0.69
R7717:Vmn2r2	UTSW	3	64,042,019 (GRCm39)	missense	possibly damaging	0.95
R7798:Vmn2r2	UTSW	3	64,041,518 (GRCm39)	missense	possibly damaging	0.60
R7914:Vmn2r2	UTSW	3	64,041,526 (GRCm39)	missense	probably benign	0.20
R7974:Vmn2r2	UTSW	3	64,024,808 (GRCm39)	missense	probably damaging	0.99
R8394:Vmn2r2	UTSW	3	64,044,858 (GRCm39)	missense	probably damaging	1.00
R8478:Vmn2r2	UTSW	3	64,024,257 (GRCm39)	missense	possibly damaging	0.87
R8731:Vmn2r2	UTSW	3	64,024,404 (GRCm39)	missense	probably benign	0.16
R9035:Vmn2r2	UTSW	3	64,024,172 (GRCm39)	missense	probably damaging	1.00
R9182:Vmn2r2	UTSW	3	64,044,802 (GRCm39)	missense	probably damaging	1.00
R9225:Vmn2r2	UTSW	3	64,034,021 (GRCm39)	missense	probably benign	0.00
R9266:Vmn2r2	UTSW	3	64,024,057 (GRCm39)	missense	probably damaging	0.98
R9427:Vmn2r2	UTSW	3	64,041,945 (GRCm39)	missense	probably damaging	1.00
R9659:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
R9666:Vmn2r2	UTSW	3	64,023,870 (GRCm39)	missense	probably benign	0.08
R9771:Vmn2r2	UTSW	3	64,042,079 (GRCm39)	missense	possibly damaging	0.88
R9788:Vmn2r2	UTSW	3	64,041,942 (GRCm39)	missense	possibly damaging	0.79
X0024:Vmn2r2	UTSW	3	64,044,707 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- GCTCCAACACCAAGTATGCTG -3'
(R):5'- AAGTGTGTGCCAAAGCTTGTG -3'

Sequencing Primer
(F):5'- CGCAGACCAGTACAATTAGTTTTCG -3'
(R):5'- GCCAAAGCTTGTGGAATTCC -3'

Posted On

2018-11-28