Mutagenetix > Incidental Mutation

Incidental Mutation 'R6963:Gm5150'

543314

Institutional Source

Beutler Lab

Gene Symbol

Gm5150

Ensembl Gene

ENSMUSG00000078780

Gene Name

predicted gene 5150

Synonyms

MMRRC Submission

045073-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R6963 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

16001035-16060535 bp(-) (GRCm39)

Type of Mutation

start gained

DNA Base Change (assembly)

A to G at 16060555 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000141397 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108347] [ENSMUST00000194367]

AlphaFold

Q1AN92

Predicted Effect

probably benign
Transcript: ENSMUST00000108347

SMART Domains

Protein: ENSMUSP00000103984
Gene: ENSMUSG00000078780

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
IG	37	143	8.78e-9	SMART
IG	156	262	7.52e-8	SMART
transmembrane domain	272	294	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000194367

SMART Domains

Protein: ENSMUSP00000141397
Gene: ENSMUSG00000078780

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
IG	37	143	8.78e-9	SMART
IG	156	262	7.52e-8	SMART
transmembrane domain	272	294	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.5%

Validation Efficiency

100% (41/41)

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aasdh	G	A	5: 77,044,303 (GRCm39)	H196Y	probably damaging	Het
Abi3	G	A	11: 95,723,567 (GRCm39)		probably benign	Het
Adgrb2	CG	C	4: 129,908,155 (GRCm39)		probably null	Het
Asgr1	T	C	11: 69,946,794 (GRCm39)		probably null	Het
Atp2c2	C	T	8: 120,457,006 (GRCm39)	R203*	probably null	Het
Brms1	T	A	19: 5,096,681 (GRCm39)	I121N	probably damaging	Het
Ccdc149	G	A	5: 52,596,439 (GRCm39)	R58W	probably damaging	Het
D630003M21Rik	T	C	2: 158,042,228 (GRCm39)	E906G	probably benign	Het
Enpp5	G	A	17: 44,396,155 (GRCm39)	G356S	probably damaging	Het
Fry	A	G	5: 150,381,309 (GRCm39)	T444A	probably benign	Het
Ggn	A	G	7: 28,871,007 (GRCm39)	E142G	probably damaging	Het
Gm57858	A	G	3: 36,104,811 (GRCm39)	Y17H	probably benign	Het
Gp2	A	G	7: 119,052,120 (GRCm39)	V198A	probably benign	Het
Gstm3	A	G	3: 107,874,940 (GRCm39)	V104A	probably benign	Het
Idua	A	G	5: 108,827,641 (GRCm39)	K152E	possibly damaging	Het
Igsf21	A	G	4: 139,755,041 (GRCm39)	S443P	probably benign	Het
Kdm5d	C	A	Y: 937,975 (GRCm39)	Q925K	probably benign	Het
Ly6k	G	C	15: 74,670,431 (GRCm39)	P37R	probably damaging	Het
Mcm9	A	G	10: 53,424,713 (GRCm39)	S626P	probably damaging	Het
Mcoln2	A	G	3: 145,877,790 (GRCm39)	K137R	probably damaging	Het
Mctp2	T	C	7: 71,877,804 (GRCm39)	N298S	probably damaging	Het
Myo10	T	C	15: 25,734,149 (GRCm39)	I379T	probably benign	Het
Myo15b	G	T	11: 115,781,540 (GRCm39)		probably null	Het
Nrg1	A	G	8: 32,407,690 (GRCm39)	F181S	probably benign	Het
Or5b24	C	T	19: 12,913,002 (GRCm39)	A300V	probably damaging	Het
Pals2	T	C	6: 50,140,635 (GRCm39)		probably null	Het
Pde9a	G	T	17: 31,662,861 (GRCm39)	V97L	probably benign	Het
Rfc5	T	A	5: 117,525,931 (GRCm39)		probably null	Het
Rnf145	T	C	11: 44,455,104 (GRCm39)	S662P	probably benign	Het
Rsf1	G	A	7: 97,229,117 (GRCm39)		probably benign	Het
Scfd2	A	G	5: 74,642,870 (GRCm39)	V359A	probably damaging	Het
Skp2	T	C	15: 9,139,515 (GRCm39)		probably null	Het
St3gal1	A	G	15: 66,983,195 (GRCm39)	V187A	possibly damaging	Het
Tekt2	T	C	4: 126,218,110 (GRCm39)	E134G	probably damaging	Het
Ttll4	T	A	1: 74,720,975 (GRCm39)	I547K	probably damaging	Het
Vmn1r4	T	C	6: 56,933,769 (GRCm39)	I91T	probably damaging	Het
Vmn2r93	T	C	17: 18,536,849 (GRCm39)	S511P	probably damaging	Het
Vps50	T	C	6: 3,592,577 (GRCm39)		probably null	Het
Zeb2	T	G	2: 44,878,811 (GRCm39)	E1141A	probably damaging	Het
Zfp1002	A	G	2: 150,097,265 (GRCm39)	C55R	probably damaging	Het
Zfp326	T	A	5: 106,059,359 (GRCm39)	Y373*	probably null	Het

Other mutations in Gm5150

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02188:Gm5150	APN	3	16,017,826 (GRCm39)	missense	possibly damaging	0.95
IGL02485:Gm5150	APN	3	16,044,916 (GRCm39)	missense	probably damaging	1.00
IGL02507:Gm5150	APN	3	16,017,485 (GRCm39)	missense	probably damaging	0.97
R1760:Gm5150	UTSW	3	16,060,468 (GRCm39)	missense	probably benign	0.00
R2081:Gm5150	UTSW	3	16,045,109 (GRCm39)	missense	probably benign	0.29
R3080:Gm5150	UTSW	3	16,045,085 (GRCm39)	missense	possibly damaging	0.93
R3149:Gm5150	UTSW	3	16,060,479 (GRCm39)	missense	probably damaging	0.99
R5329:Gm5150	UTSW	3	16,017,588 (GRCm39)	missense	probably benign	0.35
R5627:Gm5150	UTSW	3	16,017,564 (GRCm39)	missense	probably damaging	1.00
R6052:Gm5150	UTSW	3	16,044,917 (GRCm39)	missense	probably damaging	1.00
R6455:Gm5150	UTSW	3	16,044,815 (GRCm39)	missense	probably damaging	1.00
R7688:Gm5150	UTSW	3	16,017,747 (GRCm39)	missense	probably benign	0.07
R7872:Gm5150	UTSW	3	16,060,485 (GRCm39)	start codon destroyed	probably null	0.37
R8368:Gm5150	UTSW	3	16,044,902 (GRCm39)	missense	probably damaging	1.00
R9165:Gm5150	UTSW	3	16,045,060 (GRCm39)	missense	probably damaging	1.00
R9629:Gm5150	UTSW	3	16,044,829 (GRCm39)	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- CTCAGTGTCAGGGAGATGATTCTG -3'
(R):5'- ACTTGAGACAACATGGCACCAG -3'

Sequencing Primer
(F):5'- GTTGTTGGGGCATGCTCACC -3'
(R):5'- ATAGGAACTCCTTCCCAGC -3'

Posted On

2018-11-28