Mutagenetix > Incidental Mutation

Incidental Mutation 'R6969:Dusp10'

543352

Institutional Source

Beutler Lab

Gene Symbol

Dusp10

Ensembl Gene

ENSMUSG00000039384

Gene Name

dual specificity phosphatase 10

Synonyms

MKP5, 2610306G15Rik, MKP-5

MMRRC Submission

045079-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.719) question?

Stock #

R6969 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

183766575-183807833 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 183801085 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 284 (L284P)

Ref Sequence

ENSEMBL: ENSMUSP00000045838 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048655]

AlphaFold

Q9ESS0

Predicted Effect

probably damaging
Transcript: ENSMUST00000048655
AA Change: L284P

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000045838
Gene: ENSMUSG00000039384
AA Change: L284P

Domain	Start	End	E-Value	Type
low complexity region	33	59	N/A	INTRINSIC
low complexity region	92	111	N/A	INTRINSIC
low complexity region	124	142	N/A	INTRINSIC
RHOD	159	283	1.71e-11	SMART
DSPc	322	462	1.43e-54	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.8%
10x: 99.0%
20x: 96.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for a knock-out allele display alterations in both innate and adaptive immune responses. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aak1	T	A	6: 86,958,317 (GRCm39)	L800H	unknown	Het
Ap2b1	T	A	11: 83,280,552 (GRCm39)	D788E	probably damaging	Het
Arfgef1	G	T	1: 10,223,904 (GRCm39)	Q1465K	probably damaging	Het
Arfgef1	T	C	1: 10,223,903 (GRCm39)	Q1465R	probably damaging	Het
Arhgap9	G	A	10: 127,162,512 (GRCm39)	E348K	probably benign	Het
B4galnt2	A	T	11: 95,782,756 (GRCm39)	F19I	probably benign	Het
Bdp1	A	T	13: 100,211,039 (GRCm39)	I551N	probably damaging	Het
Ceacam16	A	G	7: 19,586,230 (GRCm39)	*427Q	probably null	Het
Chd9	A	C	8: 91,705,542 (GRCm39)	Q260P	probably benign	Het
Col16a1	A	T	4: 129,986,880 (GRCm39)		probably benign	Het
Csmd1	A	T	8: 17,266,805 (GRCm39)	N40K	possibly damaging	Het
Depdc5	T	G	5: 33,141,204 (GRCm39)	V1368G	probably damaging	Het
Dnah7b	C	A	1: 46,397,398 (GRCm39)	P3943Q	probably damaging	Het
Dnttip2	A	G	3: 122,076,141 (GRCm39)	Q691R	probably damaging	Het
Efr3b	A	G	12: 4,018,624 (GRCm39)	V574A	probably benign	Het
Erc2	A	T	14: 27,620,553 (GRCm39)	I60F	probably damaging	Het
Exoc2	A	G	13: 31,095,161 (GRCm39)	V245A	probably benign	Het
Fasl	G	T	1: 161,609,244 (GRCm39)	F37L	probably damaging	Het
Fat3	G	A	9: 15,941,212 (GRCm39)	P1360S	probably benign	Het
Gpsm1	C	T	2: 26,230,555 (GRCm39)	P502S	probably benign	Het
Gtpbp10	C	A	5: 5,605,331 (GRCm39)	G124V	probably damaging	Het
Insm2	T	C	12: 55,646,963 (GRCm39)	C236R	probably damaging	Het
Irf2bpl	A	G	12: 86,929,468 (GRCm39)	Y402H	possibly damaging	Het
Irx6	A	G	8: 93,403,958 (GRCm39)	E175G	probably damaging	Het
Kcnh8	C	T	17: 53,184,971 (GRCm39)	R418*	probably null	Het
Kif3c	G	A	12: 3,416,114 (GRCm39)	R45Q	probably benign	Het
Larp7-ps	A	G	4: 92,079,826 (GRCm39)	I54T	probably damaging	Het
Lpin1	A	G	12: 16,630,862 (GRCm39)	F12S	probably damaging	Het
Lrba	A	T	3: 86,526,897 (GRCm39)	T156S	probably benign	Het
Lrrc19	G	T	4: 94,527,610 (GRCm39)	N200K	probably benign	Het
Lrrc7	G	A	3: 157,862,550 (GRCm39)	H1296Y	probably benign	Het
Ltn1	A	T	16: 87,212,578 (GRCm39)	F661Y	probably damaging	Het
Macf1	T	C	4: 123,351,593 (GRCm39)	Y1893C	probably benign	Het
Mmd	G	C	11: 90,148,362 (GRCm39)	A15P	probably damaging	Het
Myh2	T	C	11: 67,088,092 (GRCm39)	F1903L	probably benign	Het
Myom3	T	C	4: 135,528,371 (GRCm39)	L1072P	probably damaging	Het
Or13l2	A	T	3: 97,318,118 (GRCm39)	Y126*	probably null	Het
Or56a41	T	C	7: 104,740,463 (GRCm39)	I128V	probably benign	Het
Or5bw2	G	A	7: 6,573,320 (GRCm39)	C110Y	probably damaging	Het
Or7g35	A	T	9: 19,495,886 (GRCm39)	T18S	possibly damaging	Het
Patl2	A	T	2: 121,959,410 (GRCm39)	V18D	possibly damaging	Het
Pkn1	T	C	8: 84,410,055 (GRCm39)	S395G	probably damaging	Het
Ptprm	A	G	17: 67,219,413 (GRCm39)	I726T	possibly damaging	Het
Rab3gap2	T	C	1: 184,968,209 (GRCm39)	L187P	probably damaging	Het
Ric1	A	T	19: 29,563,182 (GRCm39)	E535V	probably damaging	Het
Ripor3	T	C	2: 167,827,657 (GRCm39)	K598R	probably benign	Het
Rnf40	A	G	7: 127,195,495 (GRCm39)	E607G	possibly damaging	Het
Rsf1	ATGGCG	ATGGCGACGGTGGCG	7: 97,229,111 (GRCm39)		probably benign	Het
Sbsn	A	G	7: 30,452,616 (GRCm39)	T544A	probably benign	Het
Scaf1	C	A	7: 44,657,253 (GRCm39)		probably benign	Het
Sec24a	T	C	11: 51,591,643 (GRCm39)	M1018V	probably benign	Het
Shmt1	C	T	11: 60,695,153 (GRCm39)	A54T	probably damaging	Het
Slc39a14	C	A	14: 70,546,275 (GRCm39)	V383F	probably damaging	Het
Slc5a2	A	G	7: 127,871,249 (GRCm39)	T346A	probably benign	Het
Slco4a1	G	A	2: 180,106,601 (GRCm39)	S261N	probably benign	Het
Smarcc1	C	G	9: 110,025,388 (GRCm39)	S688R	probably damaging	Het
Sppl2b	G	A	10: 80,700,959 (GRCm39)	A314T	probably damaging	Het
Sptb	A	T	12: 76,654,781 (GRCm39)	V1513E	probably damaging	Het
Stx17	A	T	4: 48,140,462 (GRCm39)	I56F	probably damaging	Het
Tbc1d9	A	G	8: 83,968,171 (GRCm39)	Y424C	probably damaging	Het
Tgm3	A	G	2: 129,883,949 (GRCm39)	K536E	probably benign	Het
Tti2	A	G	8: 31,644,329 (GRCm39)	I309V	possibly damaging	Het
Tymp	G	A	15: 89,258,251 (GRCm39)	S334L	probably benign	Het
Unc13b	T	C	4: 43,263,538 (GRCm39)	F1587L	possibly damaging	Het
Vgf	G	T	5: 137,060,507 (GRCm39)		probably benign	Het
Zfp59	T	C	7: 27,552,922 (GRCm39)	S125P	probably damaging	Het
Zfp641	A	T	15: 98,188,448 (GRCm39)	M144K	possibly damaging	Het
Zfp93	A	T	7: 23,974,806 (GRCm39)	K264*	probably null	Het

Other mutations in Dusp10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00335:Dusp10	APN	1	183,801,328 (GRCm39)	missense	probably benign	0.00
IGL01094:Dusp10	APN	1	183,769,697 (GRCm39)	splice site	probably null
IGL01380:Dusp10	APN	1	183,801,211 (GRCm39)	missense	possibly damaging	0.93
FR4449:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4548:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4737:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4976:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
LCD18:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
R0369:Dusp10	UTSW	1	183,801,253 (GRCm39)	missense	probably damaging	1.00
R0433:Dusp10	UTSW	1	183,801,393 (GRCm39)	missense	probably damaging	1.00
R0464:Dusp10	UTSW	1	183,801,273 (GRCm39)	missense	probably benign	0.01
R1112:Dusp10	UTSW	1	183,769,097 (GRCm39)	missense	probably damaging	0.98
R1474:Dusp10	UTSW	1	183,769,645 (GRCm39)	splice site	probably null
R1667:Dusp10	UTSW	1	183,769,055 (GRCm39)	missense	probably damaging	1.00
R1719:Dusp10	UTSW	1	183,769,422 (GRCm39)	missense	probably benign	0.22
R1899:Dusp10	UTSW	1	183,801,377 (GRCm39)	missense	possibly damaging	0.64
R5238:Dusp10	UTSW	1	183,769,210 (GRCm39)	missense	possibly damaging	0.94
R5277:Dusp10	UTSW	1	183,769,204 (GRCm39)	missense	possibly damaging	0.94
R5742:Dusp10	UTSW	1	183,769,853 (GRCm39)	splice site	probably null
R5948:Dusp10	UTSW	1	183,801,073 (GRCm39)	missense	probably benign
R6890:Dusp10	UTSW	1	183,801,393 (GRCm39)	missense	probably damaging	1.00
R7007:Dusp10	UTSW	1	183,769,414 (GRCm39)	missense	probably benign	0.22
R7033:Dusp10	UTSW	1	183,769,802 (GRCm39)	missense	possibly damaging	0.94
R7436:Dusp10	UTSW	1	183,801,418 (GRCm39)	missense	probably damaging	1.00
R7447:Dusp10	UTSW	1	183,801,153 (GRCm39)	missense	probably benign
R7479:Dusp10	UTSW	1	183,769,617 (GRCm39)	missense	probably damaging	0.99
R7572:Dusp10	UTSW	1	183,806,506 (GRCm39)	missense	probably damaging	1.00
R8191:Dusp10	UTSW	1	183,769,749 (GRCm39)	missense	possibly damaging	0.89
R8201:Dusp10	UTSW	1	183,769,202 (GRCm39)	missense	possibly damaging	0.51
R9429:Dusp10	UTSW	1	183,801,091 (GRCm39)	missense	probably benign	0.01
R9466:Dusp10	UTSW	1	183,769,234 (GRCm39)	missense	probably damaging	1.00
R9593:Dusp10	UTSW	1	183,806,643 (GRCm39)	missense	probably damaging	0.99
Z1177:Dusp10	UTSW	1	183,801,189 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- CTGGCTCTGGGTATCCAAACAG -3'
(R):5'- TCTGCATGGTGTCTAGGTCC -3'

Sequencing Primer
(F):5'- TGGGTATCCAAACAGGCTTC -3'
(R):5'- GTGTCTAGGTCCTGAGCATCC -3'

Posted On

2018-11-28