Mutagenetix > Incidental Mutation

Incidental Mutation 'R6711:Or8g22'

543576

Institutional Source

Beutler Lab

Gene Symbol

Or8g22

Ensembl Gene

ENSMUSG00000095194

Gene Name

olfactory receptor family 8 subfamily G member 22, pseudogene 1

Synonyms

Olfr936, MOR171-37, EG628171, GA_x6K02T2PVTD-32743332-32742397

MMRRC Submission

044829-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R6711 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38957910-38958845 bp(-) (GRCm39)

Type of Mutation

makesense

DNA Base Change (assembly)

A to G at 38958162 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Stop codon to Glutamine at position 229 (*229Q)

Ref Sequence

ENSEMBL: ENSMUSP00000148891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000178303] [ENSMUST00000216912]

AlphaFold

J3KMV2

Predicted Effect

silent
Transcript: ENSMUST00000178303

SMART Domains

Protein: ENSMUSP00000137209
Gene: ENSMUSG00000095194

Domain	Start	End	E-Value	Type
Pfam:7tm_1	1	246	6e-21	PFAM
Pfam:7tm_4	1	264	6.9e-49	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000216912
AA Change: *229Q

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.1%
20x: 94.5%

Validation Efficiency

100% (47/47)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Feb 2010]

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akr1c21	A	G	13: 4,627,374 (GRCm39)	D156G	probably damaging	Het
Ankrd35	C	T	3: 96,590,784 (GRCm39)	Q357*	probably null	Het
Ano2	G	A	6: 125,752,795 (GRCm39)	A191T	probably damaging	Het
Cacna2d1	C	T	5: 16,505,039 (GRCm39)	T331I	probably damaging	Het
Ccdc81	T	G	7: 89,537,006 (GRCm39)	E214A	probably damaging	Het
Cdh4	A	G	2: 179,532,724 (GRCm39)	T729A	probably damaging	Het
Ceacam2	A	G	7: 25,238,295 (GRCm39)	L43P	probably benign	Het
Ces2h	A	T	8: 105,744,715 (GRCm39)	R364S	probably benign	Het
Dcpp3	AGGCCATGCTGGCC	AGGCC	17: 24,136,572 (GRCm39)		probably benign	Het
Entrep1	T	C	19: 23,955,463 (GRCm39)	N385S	probably benign	Het
Epb42	T	A	2: 120,854,589 (GRCm39)		probably benign	Het
Fcgbp	G	A	7: 27,789,098 (GRCm39)	V555M	probably damaging	Het
Ganc	A	T	2: 120,281,320 (GRCm39)	H723L	possibly damaging	Het
Gfra2	A	G	14: 71,203,715 (GRCm39)	D31G	probably damaging	Het
Glrb	A	G	3: 80,752,281 (GRCm39)	I443T	probably benign	Het
Hcfc1	A	T	X: 72,993,671 (GRCm39)	C1165S	probably damaging	Homo
Hoxd10	A	G	2: 74,524,507 (GRCm39)	Y273C	probably damaging	Het
Iba57	T	C	11: 59,049,369 (GRCm39)	T267A	probably damaging	Het
Impg2	C	A	16: 56,085,449 (GRCm39)	P943H	probably damaging	Het
Kidins220	A	G	12: 25,048,750 (GRCm39)	T145A	probably damaging	Het
Lmntd1	T	C	6: 145,489,228 (GRCm39)	Y11C	probably benign	Het
Lyst	A	T	13: 13,809,820 (GRCm39)	T497S	possibly damaging	Het
Man1a	G	A	10: 53,809,588 (GRCm39)	H406Y	probably benign	Het
Mme	A	T	3: 63,249,339 (GRCm39)	K289N	possibly damaging	Het
Mrps21	G	A	3: 95,777,895 (GRCm39)		probably benign	Het
Mtor	A	G	4: 148,536,824 (GRCm39)	N33D	possibly damaging	Het
Ncoa1	C	A	12: 4,372,904 (GRCm39)	A166S	probably benign	Het
Ndrg2	A	T	14: 52,147,782 (GRCm39)	F112I	possibly damaging	Het
Neb	A	C	2: 52,113,076 (GRCm39)	F36C	probably benign	Het
Neb	A	G	2: 52,146,299 (GRCm39)	Y2893H	probably damaging	Het
Pcdh15	A	T	10: 74,478,219 (GRCm39)	E231D	possibly damaging	Het
Pih1d2	T	A	9: 50,529,310 (GRCm39)	M1K	probably null	Het
Pla2g4e	T	C	2: 120,001,751 (GRCm39)	N633D	probably benign	Het
Rph3al	C	A	11: 75,799,810 (GRCm39)	G50*	probably null	Het
Rtcb	A	T	10: 85,774,963 (GRCm39)	N477K	possibly damaging	Het
Scn10a	A	G	9: 119,438,979 (GRCm39)	F1630S	probably damaging	Het
Srfbp1	T	C	18: 52,621,373 (GRCm39)	S145P	probably damaging	Het
St7	A	G	6: 17,848,069 (GRCm39)	E211G	possibly damaging	Het
Thbs2	T	C	17: 14,910,527 (GRCm39)	D24G	probably benign	Het
Tlr6	C	A	5: 65,111,835 (GRCm39)	M357I	probably damaging	Het
Tmem168	T	C	6: 13,603,120 (GRCm39)	Y82C	probably damaging	Het
Tnrc18	A	T	5: 142,773,545 (GRCm39)	L245Q	unknown	Het
Vmn1r8	T	C	6: 57,013,444 (GRCm39)	L165P	probably damaging	Het
Vps13b	A	G	15: 35,887,395 (GRCm39)	Y3268C	probably damaging	Het
Zdhhc7	A	G	8: 120,810,066 (GRCm39)	I218T	probably benign	Het
Zfp213	A	G	17: 23,778,485 (GRCm39)	F209S	probably benign	Het

Other mutations in Or8g22

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01928:Or8g22	APN	9	38,958,709 (GRCm39)	missense	unknown
R0086:Or8g22	UTSW	9	38,958,191 (GRCm39)	missense	probably benign	0.00
R0561:Or8g22	UTSW	9	38,958,669 (GRCm39)	missense	probably damaging	0.99
R0650:Or8g22	UTSW	9	38,957,996 (GRCm39)	missense	probably benign	0.01
R1221:Or8g22	UTSW	9	38,958,483 (GRCm39)	missense	probably damaging	1.00
R1384:Or8g22	UTSW	9	38,958,200 (GRCm39)	missense	possibly damaging	0.70
R1680:Or8g22	UTSW	9	38,958,296 (GRCm39)	missense	probably benign	0.43
R1733:Or8g22	UTSW	9	38,958,678 (GRCm39)	missense	unknown
R3767:Or8g22	UTSW	9	38,958,707 (GRCm39)	missense	unknown
R4786:Or8g22	UTSW	9	38,958,783 (GRCm39)	nonsense	probably null
R4944:Or8g22	UTSW	9	38,958,158 (GRCm39)	missense	probably damaging	1.00
R5186:Or8g22	UTSW	9	38,958,265 (GRCm39)	nonsense	probably null
R5403:Or8g22	UTSW	9	38,957,999 (GRCm39)	missense	probably damaging	1.00
R6037:Or8g22	UTSW	9	38,958,403 (GRCm39)	missense	probably damaging	1.00
R6037:Or8g22	UTSW	9	38,958,403 (GRCm39)	missense	probably damaging	1.00
R6156:Or8g22	UTSW	9	38,958,671 (GRCm39)	missense	possibly damaging	0.90
R6217:Or8g22	UTSW	9	38,958,039 (GRCm39)	makesense	probably null
R6919:Or8g22	UTSW	9	38,958,827 (GRCm39)	utr 5 prime	probably benign
R7022:Or8g22	UTSW	9	38,958,379 (GRCm39)	nonsense	probably null
R7275:Or8g22	UTSW	9	38,958,815 (GRCm39)	utr 5 prime	probably benign
R7290:Or8g22	UTSW	9	38,958,694 (GRCm39)	missense	unknown
R7644:Or8g22	UTSW	9	38,958,638 (GRCm39)	missense	probably damaging	1.00
R8906:Or8g22	UTSW	9	38,958,077 (GRCm39)	missense	possibly damaging	0.87
R9099:Or8g22	UTSW	9	38,958,026 (GRCm39)	missense	probably benign	0.00
Z1176:Or8g22	UTSW	9	38,958,215 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GTTCCTCAGACTGTAGATTAGGG -3'
(R):5'- GCATCAGCTCATGTAGGGTG -3'

Sequencing Primer
(F):5'- TTAGGGGGTTGAGCATAGGCAC -3'
(R):5'- TCTGCAGATTTGATGTCATTAACC -3'

Posted On

2019-03-12