Mutagenetix > Incidental Mutation

Incidental Mutation 'R6727:Prl3d3'

543589

Institutional Source

Beutler Lab

Gene Symbol

Prl3d3

Ensembl Gene

ENSMUSG00000062201

Gene Name

prolactin family 3, subfamily d, member 3

Synonyms

Plig, PL-Ig

MMRRC Submission

044845-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R6727 (G1)

Quality Score

213.009

Status

Validated

Chromosome

Chromosomal Location

27340777-27346576 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to A at 27341147 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000073286 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073601] [ENSMUST00000223958]

AlphaFold

A0A0M6L0J6

Predicted Effect

probably null
Transcript: ENSMUST00000073601

SMART Domains

Protein: ENSMUSP00000073286
Gene: ENSMUSG00000062201

Domain	Start	End	E-Value	Type
Pfam:Hormone_1	16	223	9.9e-72	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000223958

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 96.1%

Validation Efficiency

98% (44/45)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010109A12Rik	A	G	5: 93,354,434 (GRCm39)		probably benign	Het
4930563M21Rik	C	T	9: 55,896,760 (GRCm39)	V283I	possibly damaging	Het
Acot11	C	T	4: 106,617,327 (GRCm39)	G240R	probably damaging	Het
Allc	T	A	12: 28,607,388 (GRCm39)	H288L	probably damaging	Het
Atg16l1	T	C	1: 87,702,576 (GRCm39)	I279T	possibly damaging	Het
Atp6v1b1	A	G	6: 83,728,857 (GRCm39)		probably benign	Het
Barhl1	G	A	2: 28,805,495 (GRCm39)	P66L	probably benign	Het
Brd8dc	T	A	18: 34,713,894 (GRCm39)	M244L	probably benign	Het
Cfap58	A	T	19: 47,943,856 (GRCm39)	D352V	probably benign	Het
Cyp3a44	T	A	5: 145,731,781 (GRCm39)	K122*	probably null	Het
Dnai1	G	T	4: 41,625,308 (GRCm39)	R424L	probably benign	Het
Dync1li2	G	T	8: 105,167,167 (GRCm39)	H79Q	probably damaging	Het
Fem1b	A	G	9: 62,704,015 (GRCm39)	V415A	possibly damaging	Het
Fgb	C	T	3: 82,954,094 (GRCm39)	S48N	possibly damaging	Het
Gm5624	T	C	14: 44,799,332 (GRCm39)	D31G	possibly damaging	Het
Gzmn	T	A	14: 56,403,432 (GRCm39)	I226F	probably damaging	Het
H2-T5	A	T	17: 36,476,622 (GRCm39)	V284E	probably damaging	Het
Il31ra	T	C	13: 112,683,902 (GRCm39)	S184G	probably damaging	Het
Insrr	C	T	3: 87,720,873 (GRCm39)	R1044C	probably damaging	Het
Kcnj15	A	G	16: 95,097,193 (GRCm39)	S272G	probably damaging	Het
Kcnk16	C	T	14: 20,312,997 (GRCm39)	A106T	probably benign	Het
Kmt2b	A	G	7: 30,283,984 (GRCm39)	V876A	probably damaging	Het
Large2	G	T	2: 92,201,215 (GRCm39)		probably benign	Het
Maml2	A	T	9: 13,532,847 (GRCm39)		probably benign	Het
Me1	A	G	9: 86,464,851 (GRCm39)	L533P	possibly damaging	Het
Muc16	A	G	9: 18,477,986 (GRCm39)		probably null	Het
Nova2	C	A	7: 18,692,419 (GRCm39)	T516K	probably damaging	Het
Or1l4	T	A	2: 37,092,118 (GRCm39)	N288K	probably damaging	Het
Or56b1	T	C	7: 104,285,094 (GRCm39)	I71T	probably damaging	Het
Otogl	G	A	10: 107,612,978 (GRCm39)		silent	Het
Ppp2r1a	T	A	17: 21,176,087 (GRCm39)	V103E	probably benign	Het
Rhbdf1	G	T	11: 32,164,042 (GRCm39)	A288E	possibly damaging	Het
Rnf213	T	C	11: 119,321,147 (GRCm39)	S1202P	possibly damaging	Het
Slc25a17	A	G	15: 81,222,154 (GRCm39)	V106A	probably benign	Het
Slc4a4	T	G	5: 89,318,624 (GRCm39)	S640A	probably benign	Het
Smc4	T	A	3: 68,924,105 (GRCm39)	Y298N	probably damaging	Het
Tek	G	T	4: 94,741,732 (GRCm39)	G830*	probably null	Het
Tgfb1	A	T	7: 25,388,587 (GRCm39)		probably benign	Het
Themis	T	C	10: 28,657,903 (GRCm39)	I157T	probably damaging	Het
Trmt12	A	G	15: 58,744,514 (GRCm39)		probably benign	Het
Trrap	T	C	5: 144,793,760 (GRCm39)	W3654R	probably damaging	Het
Tspan3	C	T	9: 56,054,724 (GRCm39)	G108S	probably damaging	Het
Ugt1a10	T	A	1: 87,983,979 (GRCm39)		probably null	Het
Vps13b	A	G	15: 35,770,829 (GRCm39)	K2091E	probably benign	Het
Wdr62	A	T	7: 29,971,045 (GRCm39)	V184D	probably damaging	Het
Zfp958	C	A	8: 4,678,247 (GRCm39)	Q90K	probably benign	Het

Other mutations in Prl3d3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00674:Prl3d3	APN	13	27,343,114 (GRCm39)	critical splice donor site	probably null
IGL01124:Prl3d3	APN	13	27,343,090 (GRCm39)	missense	possibly damaging	0.91
R0627:Prl3d3	UTSW	13	27,340,830 (GRCm39)	missense	probably damaging	0.97
R2063:Prl3d3	UTSW	13	27,346,304 (GRCm39)	missense	probably benign	0.00
R4989:Prl3d3	UTSW	13	27,343,072 (GRCm39)	missense	possibly damaging	0.91
R5665:Prl3d3	UTSW	13	27,343,064 (GRCm39)	splice site	probably null
R6254:Prl3d3	UTSW	13	27,341,453 (GRCm39)	missense	possibly damaging	0.93
R6682:Prl3d3	UTSW	13	27,345,023 (GRCm39)	missense	probably benign	0.44
R6793:Prl3d3	UTSW	13	27,345,044 (GRCm39)	missense	probably benign	0.01
R6953:Prl3d3	UTSW	13	27,345,029 (GRCm39)	missense	probably benign	0.17
R6979:Prl3d3	UTSW	13	27,341,545 (GRCm39)	missense	possibly damaging	0.92
R7503:Prl3d3	UTSW	13	27,345,096 (GRCm39)	missense	probably benign	0.01
R8872:Prl3d3	UTSW	13	27,346,324 (GRCm39)	missense	possibly damaging	0.63
R9258:Prl3d3	UTSW	13	27,344,931 (GRCm39)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- GCAGACATATTCAAGATGTGTTTGG -3'
(R):5'- CTGCTGTCAGAGAAACAAACTG -3'

Sequencing Primer
(F):5'- AAGATGTGTTTGGAGTGTAGATTTAC -3'
(R):5'- ACAATAGATGGATTGTCTTAGGATGG -3'

Posted On

2019-03-18