Mutagenetix > Incidental Mutation

Incidental Mutation 'R6991:Vmn2r99'

543873

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

045097-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R6991 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 19378110 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 132 (N132S)

Ref Sequence

ENSEMBL: ENSMUSP00000156067 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably benign
Transcript: ENSMUST00000176107
AA Change: N132S

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: N132S

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231989
AA Change: N132S

PolyPhen 2 Score 0.032 (Sensitivity: 0.95; Specificity: 0.82)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	T	A	7: 27,580,446 (GRCm38)	V278D	probably damaging	Het
Akap12	G	T	10: 4,357,122 (GRCm38)	E1311*	probably null	Het
Ankdd1a	T	A	9: 65,508,675 (GRCm38)	D186V	probably benign	Het
Birc6	A	G	17: 74,562,095 (GRCm38)	E346G	probably damaging	Het
Brd10	A	G	19: 29,719,108 (GRCm38)	L995S	possibly damaging	Het
Ccr7	A	C	11: 99,145,304 (GRCm38)	V264G	probably damaging	Het
Col7a1	T	C	9: 108,983,919 (GRCm38)		probably null	Het
Coq8a	T	C	1: 180,179,068 (GRCm38)	T132A	probably benign	Het
Cplane1	A	G	15: 8,252,206 (GRCm38)	E2843G	unknown	Het
Cyp2b10	G	A	7: 25,917,355 (GRCm38)	S407N	probably benign	Het
Ddi2	A	G	4: 141,685,250 (GRCm38)	V117A	probably benign	Het
Ddx42	G	A	11: 106,239,144 (GRCm38)	V421I	probably damaging	Het
Dip2c	T	A	13: 9,551,860 (GRCm38)	L285*	probably null	Het
Dip2c	C	T	13: 9,634,832 (GRCm38)	S1232F	probably damaging	Het
Dner	T	A	1: 84,476,402 (GRCm38)	R402*	probably null	Het
Dpysl3	A	T	18: 43,353,891 (GRCm38)	I317N	probably damaging	Het
Dusp23	T	A	1: 172,631,657 (GRCm38)	Y146F	probably benign	Het
Eef1a2	C	A	2: 181,148,628 (GRCm38)	V412L	possibly damaging	Het
Epha7	C	T	4: 28,821,489 (GRCm38)	T218I	probably damaging	Het
Gigyf2	T	A	1: 87,407,136 (GRCm38)	C284S	probably damaging	Het
Golm1	ACTTCTTCT	ACTTCT	13: 59,649,576 (GRCm38)		probably benign	Het
Hao2	T	A	3: 98,876,752 (GRCm38)	E327V	probably damaging	Het
Hdhd5	A	G	6: 120,510,169 (GRCm38)	V409A	probably damaging	Het
Kif9	T	C	9: 110,494,622 (GRCm38)	Y236H	probably damaging	Het
Kri1	T	C	9: 21,287,754 (GRCm38)		probably benign	Het
Lig4	A	T	8: 9,971,098 (GRCm38)	V894E	probably damaging	Het
Lrriq1	T	C	10: 103,187,458 (GRCm38)	N982S	probably damaging	Het
Mapk8	T	C	14: 33,410,884 (GRCm38)	I32V	possibly damaging	Het
Mast4	C	T	13: 102,804,647 (GRCm38)	V301I	probably damaging	Het
Mstn	T	C	1: 53,061,941 (GRCm38)	I59T	probably benign	Het
Mtrf1l	T	C	10: 5,813,384 (GRCm38)	E315G	probably damaging	Het
Notch4	G	T	17: 34,584,800 (GRCm38)	E1515*	probably null	Het
Npw	A	C	17: 24,658,055 (GRCm38)	V124G	probably benign	Het
Nxph3	A	G	11: 95,511,418 (GRCm38)	S57P	probably damaging	Het
Opn4	A	T	14: 34,593,907 (GRCm38)	L390M	probably benign	Het
Or12e10	G	A	2: 87,810,443 (GRCm38)	D208N	possibly damaging	Het
Or52e4	T	C	7: 105,056,564 (GRCm38)	I106T	probably damaging	Het
Or5b95	G	T	19: 12,680,748 (GRCm38)	L213F	probably benign	Het
Or5g23	T	C	2: 85,608,248 (GRCm38)	I221V	possibly damaging	Het
Or5p68	T	A	7: 108,346,088 (GRCm38)	N298Y	possibly damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195 (GRCm38)		probably benign	Het
Pla2g2e	G	A	4: 138,880,675 (GRCm38)	C62Y	probably damaging	Het
Plcb4	G	A	2: 135,910,194 (GRCm38)	V107M	probably damaging	Het
Pramel33	T	A	5: 93,484,374 (GRCm38)	N107I	probably damaging	Het
Prorsd1	A	C	11: 29,514,486 (GRCm38)		probably benign	Het
Prox2	A	G	12: 85,087,391 (GRCm38)	L587P	probably benign	Het
Prr22	A	G	17: 56,771,345 (GRCm38)	D166G	possibly damaging	Het
Ptprz1	G	A	6: 23,002,687 (GRCm38)	R1592Q	probably benign	Het
Rarg	C	G	15: 102,241,915 (GRCm38)	R74P	probably damaging	Het
Rcan1	A	G	16: 92,397,363 (GRCm38)	V54A	probably benign	Het
Rmdn2	A	G	17: 79,621,310 (GRCm38)		probably benign	Het
Sec16a	G	A	2: 26,430,486 (GRCm38)	R1361C	probably damaging	Het
Serpina1a	T	C	12: 103,853,833 (GRCm38)	T385A	probably benign	Het
Slc44a3	T	C	3: 121,532,165 (GRCm38)	Y12C	probably benign	Het
Smg1	C	A	7: 118,167,868 (GRCm38)		probably benign	Het
Spock3	A	G	8: 63,355,381 (GRCm38)	*437W	probably null	Het
Sugct	T	A	13: 17,554,380 (GRCm38)	Q220H	probably benign	Het
Tesk1	A	G	4: 43,447,006 (GRCm38)	T465A	probably benign	Het
Tnks	C	A	8: 34,834,493 (GRCm38)	R1274I	probably damaging	Het
Trp53bp1	C	T	2: 121,208,040 (GRCm38)	R1439H	probably damaging	Het
Vmn1r16	G	T	6: 57,322,884 (GRCm38)	S251*	probably null	Het
Vmn2r15	T	A	5: 109,293,314 (GRCm38)	Q226L	probably damaging	Het
Vmn2r67	T	G	7: 85,155,745 (GRCm38)	Y53S	possibly damaging	Het
Wdr73	T	G	7: 80,891,856 (GRCm38)	T313P	probably benign	Het
Zfp65	C	T	13: 67,708,521 (GRCm38)	R213Q	probably damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,378,854 (GRCm38)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,394,256 (GRCm38)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,382,623 (GRCm38)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,393,658 (GRCm38)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,380,115 (GRCm38)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,380,232 (GRCm38)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,378,690 (GRCm38)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,378,223 (GRCm38)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,394,343 (GRCm38)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,394,573 (GRCm38)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,379,043 (GRCm38)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,362,259 (GRCm38)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,380,060 (GRCm38)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,362,252 (GRCm38)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,377,945 (GRCm38)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,362,153 (GRCm38)	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19,378,815 (GRCm38)	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19,377,991 (GRCm38)	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19,378,629 (GRCm38)	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19,378,708 (GRCm38)	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19,394,373 (GRCm38)	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,378,570 (GRCm38)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,379,260 (GRCm38)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,393,662 (GRCm38)	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19,362,135 (GRCm38)	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19,378,606 (GRCm38)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,379,339 (GRCm38)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,379,269 (GRCm38)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,394,146 (GRCm38)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,377,948 (GRCm38)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,378,980 (GRCm38)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,382,605 (GRCm38)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,380,031 (GRCm38)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,380,034 (GRCm38)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,380,195 (GRCm38)	missense	possibly damaging	0.52
R7060:Vmn2r99	UTSW	17	19,394,564 (GRCm38)	nonsense	probably null
R7090:Vmn2r99	UTSW	17	19,393,710 (GRCm38)	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19,379,311 (GRCm38)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,379,145 (GRCm38)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,393,817 (GRCm38)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,380,040 (GRCm38)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,393,758 (GRCm38)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,394,181 (GRCm38)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,393,660 (GRCm38)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,378,126 (GRCm38)	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19,378,627 (GRCm38)	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19,362,301 (GRCm38)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,379,301 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- TCTAGTTCAACTGTTCATGGTGAAG -3'
(R):5'- CCTTCAGTTTAAAGGCACCAATAAC -3'

Sequencing Primer
(F):5'- TGTTTTTATGCCACTGAAACAGG -3'
(R):5'- AACATGAATAAAGAGAACTGACTCTC -3'

Posted On

2019-05-13