Mutagenetix > Incidental Mutation

Incidental Mutation 'R7003:Gpr155'

544646

Institutional Source

Beutler Lab

Gene Symbol

Gpr155

Ensembl Gene

ENSMUSG00000041762

Gene Name

G protein-coupled receptor 155

Synonyms

PGR22, 1110017O10Rik, DEPDC3, F730029F15Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7003 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

73341506-73386572 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 73343617 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 816 (I816T)

Ref Sequence

ENSEMBL: ENSMUSP00000107674 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000076463] [ENSMUST00000112043] [ENSMUST00000112044]

AlphaFold

A2AWR3

Predicted Effect

probably damaging
Transcript: ENSMUST00000076463
AA Change: I816T

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000075788
Gene: ENSMUSG00000041762
AA Change: I816T

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000112043
AA Change: I816T

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000107674
Gene: ENSMUSG00000041762
AA Change: I816T

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
transmembrane domain	209	231	N/A	INTRINSIC
transmembrane domain	241	260	N/A	INTRINSIC
transmembrane domain	272	291	N/A	INTRINSIC
transmembrane domain	311	330	N/A	INTRINSIC
transmembrane domain	343	365	N/A	INTRINSIC
transmembrane domain	380	402	N/A	INTRINSIC
transmembrane domain	415	432	N/A	INTRINSIC
transmembrane domain	442	464	N/A	INTRINSIC
transmembrane domain	477	499	N/A	INTRINSIC
transmembrane domain	519	538	N/A	INTRINSIC
transmembrane domain	657	679	N/A	INTRINSIC
transmembrane domain	689	711	N/A	INTRINSIC
DEP	759	833	8.28e-12	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000112044
AA Change: I788T

PolyPhen 2 Score 0.954 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000107675
Gene: ENSMUSG00000041762
AA Change: I788T

Domain	Start	End	E-Value	Type
Pfam:Mem_trans	40	205	3.3e-15	PFAM
Pfam:Mem_trans	187	360	8.3e-11	PFAM
transmembrane domain	382	404	N/A	INTRINSIC
transmembrane domain	414	436	N/A	INTRINSIC
transmembrane domain	449	471	N/A	INTRINSIC
transmembrane domain	491	510	N/A	INTRINSIC
transmembrane domain	629	651	N/A	INTRINSIC
transmembrane domain	661	683	N/A	INTRINSIC
DEP	731	805	8.28e-12	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131430

SMART Domains

Protein: ENSMUSP00000114294
Gene: ENSMUSG00000041762

Domain	Start	End	E-Value	Type
transmembrane domain	98	120	N/A	INTRINSIC
transmembrane domain	130	152	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2410089E03Rik	T	G	15: 8,228,762	L2164R	probably damaging	Het
Actbl2	T	A	13: 111,255,956	I275N	probably damaging	Het
Actr3b	A	C	5: 25,798,463	Y21S	probably damaging	Het
Adam6b	C	T	12: 113,490,042	Q160*	probably null	Het
Adgrv1	C	T	13: 81,522,104		probably null	Het
Akr1c6	A	G	13: 4,454,515	N300D	probably benign	Het
Alox8	T	C	11: 69,191,590	D170G	possibly damaging	Het
Amhr2	A	G	15: 102,446,333	N40S	probably benign	Het
Ap2a2	A	G	7: 141,629,196	N767S	probably benign	Het
Armc3	T	C	2: 19,270,028	I358T	probably damaging	Het
Atg2b	A	G	12: 105,654,249	S732P	probably benign	Het
Atp12a	A	G	14: 56,373,380	Y327C	possibly damaging	Het
Bcr	T	C	10: 75,061,561	V179A	probably benign	Het
Cep104	T	C	4: 153,993,561	L642P	probably benign	Het
Clspn	T	A	4: 126,592,720	S1302R	possibly damaging	Het
Cmip	T	C	8: 117,384,988	F153L	probably benign	Het
D630039A03Rik	T	C	4: 57,910,521	D97G	probably damaging	Het
Dok7	A	T	5: 35,079,555	T396S	probably benign	Het
Dsel	C	T	1: 111,860,295	V837I	probably benign	Het
Etl4	C	T	2: 20,805,884	T926I	probably benign	Het
Gm7102	A	G	19: 61,175,881	S39P	possibly damaging	Het
Gpatch2l	G	A	12: 86,244,184	R47H	probably damaging	Het
Hpn	C	T	7: 31,110,942		probably benign	Het
Inpp5e	A	G	2: 26,397,865	S640P	probably benign	Het
Irs3	C	T	5: 137,645,277	V82I	probably benign	Het
Kif16b	A	C	2: 142,758,829	D461E	possibly damaging	Het
Krba1	A	G	6: 48,413,080	T592A	possibly damaging	Het
Lgsn	T	A	1: 31,203,943	S369T	possibly damaging	Het
Lrrc4b	C	T	7: 44,445,156	P83S	probably damaging	Het
Neil3	T	C	8: 53,600,966	T343A	possibly damaging	Het
Nt5e	A	G	9: 88,364,752	Y347C	probably damaging	Het
Olfr1532-ps1	A	T	7: 106,915,112	T305S	probably benign	Het
Olfr3	A	G	2: 36,813,035	I19T	possibly damaging	Het
Olfr452	C	T	6: 42,790,465	T142I	probably benign	Het
Olfr943	A	T	9: 39,184,943	Y255F	probably benign	Het
P2rx5	T	C	11: 73,167,974		probably null	Het
Phtf2	A	G	5: 20,794,401	V248A	probably benign	Het
Plekhd1	T	C	12: 80,721,960	C406R	possibly damaging	Het
Plod3	T	A	5: 136,989,644	N245K	probably damaging	Het
Polr3c	G	T	3: 96,723,638	H155Q	possibly damaging	Het
Psap	T	A	10: 60,299,497	C317S	probably damaging	Het
Rif1	A	G	2: 52,076,989	I97V	probably benign	Het
Rnf123	T	A	9: 108,063,683		probably null	Het
Rnf19a	G	A	15: 36,254,504	R303*	probably null	Het
Sdk1	G	A	5: 142,096,734	V1036I	probably benign	Het
Shc3	T	A	13: 51,466,552	Y146F	probably benign	Het
Skint6	A	G	4: 113,105,912	Y441H	probably benign	Het
Slc7a12	T	C	3: 14,505,520	I173T	probably damaging	Het
Spesp1	A	T	9: 62,282,020	S15T	possibly damaging	Het
Tarm1	A	T	7: 3,497,423		probably null	Het
Tenm3	T	C	8: 48,240,444	Y1817C	probably damaging	Het
Ttc9c	A	T	19: 8,818,540	L45Q	probably damaging	Het
Ube3a	C	T	7: 59,276,440	T322I	probably damaging	Het
Vac14	T	A	8: 110,712,798	V669E	probably damaging	Het
Vmn1r225	A	G	17: 20,503,154	M286V	probably null	Het
Zfp658	A	G	7: 43,574,748	K816E	possibly damaging	Het
Zfp846	T	C	9: 20,587,892	M1T	probably null	Het

Other mutations in Gpr155

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00731:Gpr155	APN	2	73362613	missense	probably damaging	1.00
IGL01432:Gpr155	APN	2	73351885	missense	possibly damaging	0.51
IGL01528:Gpr155	APN	2	73362423	critical splice donor site	probably null
IGL01718:Gpr155	APN	2	73382232	missense	probably benign
IGL01733:Gpr155	APN	2	73353612	splice site	probably null
IGL03342:Gpr155	APN	2	73349678	missense	probably damaging	1.00
PIT1430001:Gpr155	UTSW	2	73370138	missense	probably benign	0.08
PIT4810001:Gpr155	UTSW	2	73348263	missense	probably benign	0.08
R0226:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R0399:Gpr155	UTSW	2	73370002	missense	possibly damaging	0.91
R0445:Gpr155	UTSW	2	73370144	splice site	probably benign
R1570:Gpr155	UTSW	2	73370038	missense	possibly damaging	0.87
R1598:Gpr155	UTSW	2	73370090	missense	probably damaging	0.98
R1647:Gpr155	UTSW	2	73364164	splice site	probably null
R1648:Gpr155	UTSW	2	73364164	splice site	probably null
R1756:Gpr155	UTSW	2	73367577	missense	probably benign	0.00
R1760:Gpr155	UTSW	2	73381935	missense	probably damaging	1.00
R2033:Gpr155	UTSW	2	73348182	missense	probably benign	0.18
R2044:Gpr155	UTSW	2	73373633	missense	probably damaging	1.00
R2145:Gpr155	UTSW	2	73356658	missense	probably benign	0.01
R2172:Gpr155	UTSW	2	73382127	missense	probably benign	0.00
R2274:Gpr155	UTSW	2	73348135	critical splice donor site	probably null
R3878:Gpr155	UTSW	2	73368392	nonsense	probably null
R3924:Gpr155	UTSW	2	73370076	missense	probably damaging	1.00
R4910:Gpr155	UTSW	2	73367538	nonsense	probably null
R4950:Gpr155	UTSW	2	73382185	missense	probably benign
R5337:Gpr155	UTSW	2	73348248	missense	probably benign	0.32
R5830:Gpr155	UTSW	2	73370089	missense	possibly damaging	0.93
R5887:Gpr155	UTSW	2	73343718	nonsense	probably null
R5929:Gpr155	UTSW	2	73373667	nonsense	probably null
R6293:Gpr155	UTSW	2	73373997	missense	possibly damaging	0.47
R6553:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R6585:Gpr155	UTSW	2	73349645	missense	probably damaging	1.00
R7353:Gpr155	UTSW	2	73367491	nonsense	probably null
R7506:Gpr155	UTSW	2	73368339	missense	probably damaging	0.97
R7631:Gpr155	UTSW	2	73382947	intron	probably benign
R7753:Gpr155	UTSW	2	73382206	missense	probably benign	0.27
R7810:Gpr155	UTSW	2	73381952	missense	probably damaging	0.99
R7813:Gpr155	UTSW	2	73381985	nonsense	probably null
R7815:Gpr155	UTSW	2	73362560	missense	probably benign
R7873:Gpr155	UTSW	2	73343590	missense	possibly damaging	0.51
R8506:Gpr155	UTSW	2	73343462	missense	probably damaging	0.99
R8680:Gpr155	UTSW	2	73343695	missense	probably damaging	0.99
R8856:Gpr155	UTSW	2	73373649	missense	probably benign	0.27
R8872:Gpr155	UTSW	2	73367592	missense	probably benign	0.02
R9116:Gpr155	UTSW	2	73373765	missense	possibly damaging	0.78
R9683:Gpr155	UTSW	2	73362436	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GGAGGCTAGAGGGTATGATTCC -3'
(R):5'- TGTCATGCAGTGCTGATGCC -3'

Sequencing Primer
(F):5'- ATGATTCCTGTGGGGCTCTCTC -3'
(R):5'- CATGCAGTGCTGATGCCAGAATTAC -3'

Posted On

2019-05-13