Incidental Mutation 'R7003:Shc3'
ID 544687
Institutional Source Beutler Lab
Gene Symbol Shc3
Ensembl Gene ENSMUSG00000021448
Gene Name src homology 2 domain-containing transforming protein C3
Synonyms ShcC, Rai, N-Shc
MMRRC Submission 045108-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.073) question?
Stock # R7003 (G1)
Quality Score 225.009
Status Not validated
Chromosome 13
Chromosomal Location 51585077-51723523 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 51620588 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Phenylalanine at position 146 (Y146F)
Ref Sequence ENSEMBL: ENSMUSP00000152080 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000021898] [ENSMUST00000223543]
AlphaFold Q61120
Predicted Effect probably benign
Transcript: ENSMUST00000021898
AA Change: Y146F

PolyPhen 2 Score 0.031 (Sensitivity: 0.95; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000021898
Gene: ENSMUSG00000021448
AA Change: Y146F

DomainStartEndE-ValueType
PTB 30 194 5.36e-41 SMART
SH2 377 456 6.38e-26 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000223543
AA Change: Y146F

PolyPhen 2 Score 0.143 (Sensitivity: 0.92; Specificity: 0.86)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype PHENOTYPE: Mice homozygous for disruptions in this gene display a normal phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 57 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Actbl2 T A 13: 111,392,490 (GRCm39) I275N probably damaging Het
Actr3b A C 5: 26,003,461 (GRCm39) Y21S probably damaging Het
Adam6b C T 12: 113,453,662 (GRCm39) Q160* probably null Het
Adgrv1 C T 13: 81,670,223 (GRCm39) probably null Het
Akr1c6 A G 13: 4,504,514 (GRCm39) N300D probably benign Het
Alox8 T C 11: 69,082,416 (GRCm39) D170G possibly damaging Het
Amhr2 A G 15: 102,354,768 (GRCm39) N40S probably benign Het
Ap2a2 A G 7: 141,209,109 (GRCm39) N767S probably benign Het
Armc3 T C 2: 19,274,839 (GRCm39) I358T probably damaging Het
Atg2b A G 12: 105,620,508 (GRCm39) S732P probably benign Het
Atp12a A G 14: 56,610,837 (GRCm39) Y327C possibly damaging Het
Bcr T C 10: 74,897,393 (GRCm39) V179A probably benign Het
Cep104 T C 4: 154,078,018 (GRCm39) L642P probably benign Het
Clspn T A 4: 126,486,513 (GRCm39) S1302R possibly damaging Het
Cmip T C 8: 118,111,727 (GRCm39) F153L probably benign Het
Cplane1 T G 15: 8,258,246 (GRCm39) L2164R probably damaging Het
D630039A03Rik T C 4: 57,910,521 (GRCm39) D97G probably damaging Het
Dok7 A T 5: 35,236,899 (GRCm39) T396S probably benign Het
Dsel C T 1: 111,788,025 (GRCm39) V837I probably benign Het
Etl4 C T 2: 20,810,695 (GRCm39) T926I probably benign Het
Gpatch2l G A 12: 86,290,958 (GRCm39) R47H probably damaging Het
Gpr155 A G 2: 73,173,961 (GRCm39) I816T probably damaging Het
Hpn C T 7: 30,810,367 (GRCm39) probably benign Het
Inpp5e A G 2: 26,287,877 (GRCm39) S640P probably benign Het
Irs3 C T 5: 137,643,539 (GRCm39) V82I probably benign Het
Kif16b A C 2: 142,600,749 (GRCm39) D461E possibly damaging Het
Krba1 A G 6: 48,390,014 (GRCm39) T592A possibly damaging Het
Lgsn T A 1: 31,243,024 (GRCm39) S369T possibly damaging Het
Lrrc4b C T 7: 44,094,580 (GRCm39) P83S probably damaging Het
Mplkipl1 A G 19: 61,164,319 (GRCm39) S39P possibly damaging Het
Neil3 T C 8: 54,054,001 (GRCm39) T343A possibly damaging Het
Nt5e A G 9: 88,246,805 (GRCm39) Y347C probably damaging Het
Or1j1 A G 2: 36,703,047 (GRCm39) I19T possibly damaging Het
Or2d3b A T 7: 106,514,319 (GRCm39) T305S probably benign Het
Or2f2 C T 6: 42,767,399 (GRCm39) T142I probably benign Het
Or8g26 A T 9: 39,096,239 (GRCm39) Y255F probably benign Het
P2rx5 T C 11: 73,058,800 (GRCm39) probably null Het
Phtf2 A G 5: 20,999,399 (GRCm39) V248A probably benign Het
Plekhd1 T C 12: 80,768,734 (GRCm39) C406R possibly damaging Het
Plod3 T A 5: 137,018,498 (GRCm39) N245K probably damaging Het
Polr3c G T 3: 96,630,954 (GRCm39) H155Q possibly damaging Het
Psap T A 10: 60,135,276 (GRCm39) C317S probably damaging Het
Rif1 A G 2: 51,967,001 (GRCm39) I97V probably benign Het
Rnf123 T A 9: 107,940,882 (GRCm39) probably null Het
Rnf19a G A 15: 36,254,650 (GRCm39) R303* probably null Het
Sdk1 G A 5: 142,082,489 (GRCm39) V1036I probably benign Het
Skint6 A G 4: 112,963,109 (GRCm39) Y441H probably benign Het
Slc7a12 T C 3: 14,570,580 (GRCm39) I173T probably damaging Het
Spesp1 A T 9: 62,189,302 (GRCm39) S15T possibly damaging Het
Tarm1 A T 7: 3,545,939 (GRCm39) probably null Het
Tenm3 T C 8: 48,693,479 (GRCm39) Y1817C probably damaging Het
Ttc9c A T 19: 8,795,904 (GRCm39) L45Q probably damaging Het
Ube3a C T 7: 58,926,188 (GRCm39) T322I probably damaging Het
Vac14 T A 8: 111,439,430 (GRCm39) V669E probably damaging Het
Vmn1r225 A G 17: 20,723,416 (GRCm39) M286V probably null Het
Zfp658 A G 7: 43,224,172 (GRCm39) K816E possibly damaging Het
Zfp846 T C 9: 20,499,188 (GRCm39) M1T probably null Het
Other mutations in Shc3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00555:Shc3 APN 13 51,615,379 (GRCm39) missense probably damaging 0.98
IGL00914:Shc3 APN 13 51,634,263 (GRCm39) splice site probably benign
IGL01417:Shc3 APN 13 51,585,200 (GRCm39) missense probably benign 0.10
IGL01536:Shc3 APN 13 51,670,595 (GRCm39) missense probably damaging 1.00
Massless UTSW 13 51,597,009 (GRCm39) missense possibly damaging 0.92
Singularity UTSW 13 51,596,856 (GRCm39) splice site probably null
R0499:Shc3 UTSW 13 51,634,264 (GRCm39) splice site probably benign
R0941:Shc3 UTSW 13 51,634,242 (GRCm39) missense probably benign
R1652:Shc3 UTSW 13 51,626,875 (GRCm39) missense probably damaging 0.99
R1739:Shc3 UTSW 13 51,636,952 (GRCm39) missense probably damaging 0.97
R1750:Shc3 UTSW 13 51,603,328 (GRCm39) missense probably damaging 1.00
R1817:Shc3 UTSW 13 51,626,888 (GRCm39) missense possibly damaging 0.93
R1848:Shc3 UTSW 13 51,615,424 (GRCm39) missense probably damaging 1.00
R1991:Shc3 UTSW 13 51,596,872 (GRCm39) missense probably benign 0.01
R2103:Shc3 UTSW 13 51,596,872 (GRCm39) missense probably benign 0.01
R4426:Shc3 UTSW 13 51,634,130 (GRCm39) splice site probably null
R4434:Shc3 UTSW 13 51,603,302 (GRCm39) missense probably benign 0.00
R4823:Shc3 UTSW 13 51,605,606 (GRCm39) missense probably benign
R4933:Shc3 UTSW 13 51,596,805 (GRCm39) missense probably benign 0.03
R4998:Shc3 UTSW 13 51,596,856 (GRCm39) splice site probably null
R5153:Shc3 UTSW 13 51,615,413 (GRCm39) missense probably damaging 1.00
R5200:Shc3 UTSW 13 51,670,601 (GRCm39) missense probably damaging 1.00
R5659:Shc3 UTSW 13 51,670,630 (GRCm39) missense probably damaging 1.00
R6035:Shc3 UTSW 13 51,615,468 (GRCm39) missense probably damaging 1.00
R6035:Shc3 UTSW 13 51,615,468 (GRCm39) missense probably damaging 1.00
R6346:Shc3 UTSW 13 51,605,651 (GRCm39) missense possibly damaging 0.93
R6434:Shc3 UTSW 13 51,603,326 (GRCm39) missense probably damaging 1.00
R6457:Shc3 UTSW 13 51,636,915 (GRCm39) splice site probably null
R6580:Shc3 UTSW 13 51,596,809 (GRCm39) missense probably benign
R6597:Shc3 UTSW 13 51,597,009 (GRCm39) missense possibly damaging 0.92
R6906:Shc3 UTSW 13 51,620,595 (GRCm39) missense probably damaging 0.97
R7104:Shc3 UTSW 13 51,585,241 (GRCm39) missense possibly damaging 0.89
R7420:Shc3 UTSW 13 51,585,271 (GRCm39) missense probably benign 0.02
R7476:Shc3 UTSW 13 51,602,042 (GRCm39) missense probably benign
R8312:Shc3 UTSW 13 51,596,754 (GRCm39) missense probably damaging 1.00
R8774:Shc3 UTSW 13 51,615,475 (GRCm39) missense probably damaging 1.00
R8774-TAIL:Shc3 UTSW 13 51,615,475 (GRCm39) missense probably damaging 1.00
R8850:Shc3 UTSW 13 51,634,248 (GRCm39) missense probably benign 0.04
Z1177:Shc3 UTSW 13 51,596,964 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- AGCATCCAGTAGGTGCTCTC -3'
(R):5'- ACTAGGAAAGTGCTGGTCCG -3'

Sequencing Primer
(F):5'- TGAAGGCCTCAATTAGCCTG -3'
(R):5'- CCTACCTCTTGCTGTGATAAG -3'
Posted On 2019-05-13