Mutagenetix > Incidental Mutation

Incidental Mutation 'R7007:Dusp10'

544751

Institutional Source

Beutler Lab

Gene Symbol

Dusp10

Ensembl Gene

ENSMUSG00000039384

Gene Name

dual specificity phosphatase 10

Synonyms

MKP5, 2610306G15Rik, MKP-5

MMRRC Submission

045109-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.719) question?

Stock #

R7007 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

183766575-183807833 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 183769414 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 127 (V127M)

Ref Sequence

ENSEMBL: ENSMUSP00000121433 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048655] [ENSMUST00000050306] [ENSMUST00000139839]

AlphaFold

Q9ESS0

Predicted Effect

probably benign
Transcript: ENSMUST00000048655
AA Change: V127M

PolyPhen 2 Score 0.225 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000045838
Gene: ENSMUSG00000039384
AA Change: V127M

Domain	Start	End	E-Value	Type
low complexity region	33	59	N/A	INTRINSIC
low complexity region	92	111	N/A	INTRINSIC
low complexity region	124	142	N/A	INTRINSIC
RHOD	159	283	1.71e-11	SMART
DSPc	322	462	1.43e-54	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000050306

SMART Domains

Protein: ENSMUSP00000055787
Gene: ENSMUSG00000044854

Domain	Start	End	E-Value	Type
SCOP:d1howa_	12	46	7e-3	SMART
low complexity region	81	115	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000139839
AA Change: V127M

PolyPhen 2 Score 0.225 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000121433
Gene: ENSMUSG00000039384
AA Change: V127M

Domain	Start	End	E-Value	Type
low complexity region	33	59	N/A	INTRINSIC
low complexity region	92	111	N/A	INTRINSIC
low complexity region	124	142	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

95% (63/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for a knock-out allele display alterations in both innate and adaptive immune responses. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930474N05Rik	C	T	14: 35,817,121 (GRCm39)	T57I	probably benign	Het
Adcy8	T	C	15: 64,576,565 (GRCm39)	N999S	possibly damaging	Het
Adgrv1	T	A	13: 81,684,483 (GRCm39)	I1073F	possibly damaging	Het
Akap3	A	G	6: 126,843,439 (GRCm39)	D686G	probably damaging	Het
Alg2	A	T	4: 47,471,881 (GRCm39)	I309N	probably benign	Het
Ankrd36	A	G	11: 5,639,168 (GRCm39)	E1360G	probably benign	Het
Aox1	C	A	1: 58,370,051 (GRCm39)	Q788K	probably damaging	Het
Apoc2	A	T	7: 19,407,282 (GRCm39)	D26E	possibly damaging	Het
Bbx	G	A	16: 50,022,851 (GRCm39)	T703I	possibly damaging	Het
C2cd4d	T	C	3: 94,271,378 (GRCm39)	Y215H	probably benign	Het
C3	C	T	17: 57,525,809 (GRCm39)	E858K	probably benign	Het
Ciita	T	C	16: 10,329,171 (GRCm39)	L482P	probably damaging	Het
Cldn9	T	C	17: 23,902,052 (GRCm39)	E191G	probably benign	Het
Cnst	T	A	1: 179,438,133 (GRCm39)	S566T	probably damaging	Het
Col5a2	A	G	1: 45,417,609 (GRCm39)	I1322T	possibly damaging	Het
Cp	G	A	3: 20,024,137 (GRCm39)	V326M	probably damaging	Het
Cyp7b1	G	A	3: 18,151,782 (GRCm39)	Q144*	probably null	Het
Dnah10	T	C	5: 124,864,490 (GRCm39)	S2232P	probably damaging	Het
Dnah17	T	C	11: 118,009,697 (GRCm39)	E625G	possibly damaging	Het
Dnah7c	T	A	1: 46,571,910 (GRCm39)	D794E	probably benign	Het
Dysf	G	C	6: 84,090,962 (GRCm39)	W1015C	probably damaging	Het
Fbxw17	T	C	13: 50,577,808 (GRCm39)	Y104H	probably damaging	Het
Gm6408	G	A	5: 146,420,647 (GRCm39)	E176K	probably damaging	Het
Gp1bb	T	A	16: 18,439,689 (GRCm39)	D135V	possibly damaging	Het
Gprin1	C	T	13: 54,886,069 (GRCm39)	C735Y	probably damaging	Het
Heatr9	T	A	11: 83,411,446 (GRCm39)	M30L	possibly damaging	Het
Hhat	G	A	1: 192,376,134 (GRCm39)	T333I	possibly damaging	Het
Htr5b	A	G	1: 121,438,223 (GRCm39)	F336S	probably damaging	Het
Ippk	T	G	13: 49,590,181 (GRCm39)		probably null	Het
Jph1	T	A	1: 17,074,410 (GRCm39)	H11L	possibly damaging	Het
Kif12	T	A	4: 63,084,717 (GRCm39)	I534L	probably benign	Het
Lemd3	A	T	10: 120,788,137 (GRCm39)	F523I	probably benign	Het
Lgsn	C	A	1: 31,229,508 (GRCm39)	H76Q	probably benign	Het
Lipm	T	A	19: 34,089,497 (GRCm39)	W152R	probably damaging	Het
Mei1	A	T	15: 81,978,200 (GRCm39)	R216W	probably damaging	Het
Mybpc1	C	T	10: 88,389,274 (GRCm39)	G379S	probably damaging	Het
Myh8	A	G	11: 67,179,142 (GRCm39)	T512A	probably benign	Het
Nf1	A	G	11: 79,337,849 (GRCm39)		probably null	Het
Npc1	T	C	18: 12,343,605 (GRCm39)	T463A	probably benign	Het
Or12e10	A	G	2: 87,640,230 (GRCm39)	N22S	probably damaging	Het
Or2y13	G	A	11: 49,415,011 (GRCm39)	V154M	probably benign	Het
Or6c7	A	T	10: 129,323,277 (GRCm39)	I133F	probably damaging	Het
Osbpl11	T	G	16: 33,047,309 (GRCm39)	I424R	possibly damaging	Het
Pnma8b	A	T	7: 16,680,181 (GRCm39)	K388N	possibly damaging	Het
Ppp1r26	A	T	2: 28,341,171 (GRCm39)	K267I	probably damaging	Het
Psmb5	A	T	14: 54,854,166 (GRCm39)	M104K	probably damaging	Het
Ptges2	T	C	2: 32,292,318 (GRCm39)	V378A	probably benign	Het
Rcan2	C	T	17: 44,147,216 (GRCm39)	S18F	probably benign	Het
Saxo5	A	T	8: 3,526,309 (GRCm39)	D154V	probably damaging	Het
Sf3b2	C	T	19: 5,324,545 (GRCm39)	R859Q	probably benign	Het
Slc7a1	G	A	5: 148,289,256 (GRCm39)			Het
Spata31d1a	T	A	13: 59,851,448 (GRCm39)	T227S	probably benign	Het
Sptbn2	T	A	19: 4,794,173 (GRCm39)	V1459E	possibly damaging	Het
Srgap2	T	C	1: 131,247,275 (GRCm39)	I586V	probably benign	Het
St6galnac1	G	A	11: 116,657,833 (GRCm39)	R356*	probably null	Het
Taf5	T	A	19: 47,059,650 (GRCm39)	F265I	probably damaging	Het
Tkfc	T	A	19: 10,573,727 (GRCm39)	I229L	probably benign	Het
Tmem132c	T	A	5: 127,436,679 (GRCm39)	L56Q	probably damaging	Het
Togaram2	C	T	17: 72,016,638 (GRCm39)	A665V	probably damaging	Het
Ttn	G	A	2: 76,537,390 (GRCm39)	T34846I	probably benign	Het
Tyr	G	A	7: 87,142,548 (GRCm39)	A4V	probably benign	Het
Ubap2	A	C	4: 41,206,221 (GRCm39)	F549L	probably damaging	Het
Usp2	T	C	9: 44,001,339 (GRCm39)	S294P	probably damaging	Het
Vrk3	A	G	7: 44,407,187 (GRCm39)	N53D	probably damaging	Het
Zfp324	T	C	7: 12,705,142 (GRCm39)	S444P	probably damaging	Het
Zfp597	T	C	16: 3,683,791 (GRCm39)	I322V	probably benign	Het

Other mutations in Dusp10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00335:Dusp10	APN	1	183,801,328 (GRCm39)	missense	probably benign	0.00
IGL01094:Dusp10	APN	1	183,769,697 (GRCm39)	splice site	probably null
IGL01380:Dusp10	APN	1	183,801,211 (GRCm39)	missense	possibly damaging	0.93
FR4449:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4548:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4737:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4976:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
LCD18:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
R0369:Dusp10	UTSW	1	183,801,253 (GRCm39)	missense	probably damaging	1.00
R0433:Dusp10	UTSW	1	183,801,393 (GRCm39)	missense	probably damaging	1.00
R0464:Dusp10	UTSW	1	183,801,273 (GRCm39)	missense	probably benign	0.01
R1112:Dusp10	UTSW	1	183,769,097 (GRCm39)	missense	probably damaging	0.98
R1474:Dusp10	UTSW	1	183,769,645 (GRCm39)	splice site	probably null
R1667:Dusp10	UTSW	1	183,769,055 (GRCm39)	missense	probably damaging	1.00
R1719:Dusp10	UTSW	1	183,769,422 (GRCm39)	missense	probably benign	0.22
R1899:Dusp10	UTSW	1	183,801,377 (GRCm39)	missense	possibly damaging	0.64
R5238:Dusp10	UTSW	1	183,769,210 (GRCm39)	missense	possibly damaging	0.94
R5277:Dusp10	UTSW	1	183,769,204 (GRCm39)	missense	possibly damaging	0.94
R5742:Dusp10	UTSW	1	183,769,853 (GRCm39)	splice site	probably null
R5948:Dusp10	UTSW	1	183,801,073 (GRCm39)	missense	probably benign
R6890:Dusp10	UTSW	1	183,801,393 (GRCm39)	missense	probably damaging	1.00
R6969:Dusp10	UTSW	1	183,801,085 (GRCm39)	missense	probably damaging	1.00
R7033:Dusp10	UTSW	1	183,769,802 (GRCm39)	missense	possibly damaging	0.94
R7436:Dusp10	UTSW	1	183,801,418 (GRCm39)	missense	probably damaging	1.00
R7447:Dusp10	UTSW	1	183,801,153 (GRCm39)	missense	probably benign
R7479:Dusp10	UTSW	1	183,769,617 (GRCm39)	missense	probably damaging	0.99
R7572:Dusp10	UTSW	1	183,806,506 (GRCm39)	missense	probably damaging	1.00
R8191:Dusp10	UTSW	1	183,769,749 (GRCm39)	missense	possibly damaging	0.89
R8201:Dusp10	UTSW	1	183,769,202 (GRCm39)	missense	possibly damaging	0.51
R9429:Dusp10	UTSW	1	183,801,091 (GRCm39)	missense	probably benign	0.01
R9466:Dusp10	UTSW	1	183,769,234 (GRCm39)	missense	probably damaging	1.00
R9593:Dusp10	UTSW	1	183,806,643 (GRCm39)	missense	probably damaging	0.99
Z1177:Dusp10	UTSW	1	183,801,189 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GTATATGCCCTCATCCAGCG -3'
(R):5'- ACAGCTCCTTGGATGTGACTC -3'

Sequencing Primer
(F):5'- TCATCCAGCGGCTCTGC -3'
(R):5'- GACTCTTGTTGTACTCCATGAAAGGC -3'

Posted On

2019-05-13