Mutagenetix > Incidental Mutation

Incidental Mutation 'R7008:Prss35'

544841

Institutional Source

Beutler Lab

Gene Symbol

Prss35

Ensembl Gene

ENSMUSG00000033491

Gene Name

protease, serine 35

Synonyms

6030424L22Rik

MMRRC Submission

045110-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.099) question?

Stock #

R7008 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

86743649-86758443 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 86756308 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 377 (V377A)

Ref Sequence

ENSEMBL: ENSMUSP00000137445 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036426] [ENSMUST00000179574]

AlphaFold

Q8C0F9

Predicted Effect

probably benign
Transcript: ENSMUST00000036426
AA Change: V377A

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000035271
Gene: ENSMUSG00000033491
AA Change: V377A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	68	83	N/A	INTRINSIC
Tryp_SPc	132	399	4.13e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000179574
AA Change: V377A

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000137445
Gene: ENSMUSG00000033491
AA Change: V377A

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	68	83	N/A	INTRINSIC
Tryp_SPc	132	399	4.13e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd17	T	A	5: 90,260,096	I1421F	possibly damaging	Het
Arhgef11	C	A	3: 87,729,218	T992K	possibly damaging	Het
Arid1b	T	C	17: 5,290,979	Y853H	probably damaging	Het
Bcl10	G	T	3: 145,933,299	R232L	probably benign	Het
Best2	T	A	8: 85,013,211	I76F	possibly damaging	Het
Card11	G	C	5: 140,873,393	R1133G	probably damaging	Het
Card9	T	C	2: 26,357,799	D180G	possibly damaging	Het
Ccdc162	T	A	10: 41,552,415	E119V	probably damaging	Het
Cd2bp2	T	C	7: 127,195,395	D15G	possibly damaging	Het
Cdk7	A	C	13: 100,717,621	M120R	probably damaging	Het
Cryba4	T	A	5: 112,251,782	T2S	probably benign	Het
Cyp19a1	A	T	9: 54,193,325	M26K	probably benign	Het
Dgcr2	A	G	16: 17,845,001	S157P	probably damaging	Het
Dis3l	A	G	9: 64,310,453	F782S	possibly damaging	Het
Ephb4	T	A	5: 137,361,274	S369T	probably benign	Het
Fhad1	CGG	CG	4: 141,918,291		probably null	Het
Gm8251	T	C	1: 44,059,625	D771G	probably benign	Het
Hist1h1e	T	C	13: 23,622,209	K97E	probably damaging	Het
Igf2bp2	A	T	16: 22,081,832	D118E	probably benign	Het
Iqgap3	GGAGAG	GGAG	3: 88,112,771		probably null	Het
Klhl5	T	C	5: 65,143,249	S52P	probably benign	Het
Lrrc28	A	T	7: 67,595,711		probably benign	Het
Map4k4	T	G	1: 39,988,971	D317E	probably benign	Het
Maz	A	T	7: 127,024,612	C66S	probably damaging	Het
Mbd4	T	C	6: 115,850,724	T43A	possibly damaging	Het
Milr1	T	A	11: 106,751,314	S11T	probably damaging	Het
Mthfd2l	T	A	5: 90,959,728	C150S	probably damaging	Het
Nemf	A	T	12: 69,341,621	N325K	possibly damaging	Het
Nemf	C	T	12: 69,353,793		probably null	Het
Nod2	T	A	8: 88,663,657	C197*	probably null	Het
Odf2l	A	G	3: 145,132,734	K241E	probably damaging	Het
Olfr1427	G	A	19: 12,098,850	T263I	possibly damaging	Het
Olfr1489	A	T	19: 13,633,621	N170I	probably damaging	Het
Osbpl10	T	G	9: 115,061,848	D101E	probably damaging	Het
Osgin1	T	C	8: 119,441,494	V20A	possibly damaging	Het
Pan3	T	A	5: 147,545,693	C438S	probably damaging	Het
Plaa	A	G	4: 94,569,349	*795Q	probably null	Het
Rab5c	G	A	11: 100,719,963	R40C	probably damaging	Het
Rasl12	G	A	9: 65,410,869	V172M	probably damaging	Het
Rdh16f1	A	G	10: 127,790,906	H276R	probably benign	Het
Runx2	G	A	17: 44,814,192	P80L	probably damaging	Het
Sez6l	T	C	5: 112,464,695	Y460C	probably damaging	Het
Sipa1l1	T	G	12: 82,363,112	M600R	probably damaging	Het
Slc11a2	T	C	15: 100,409,324	Y92C	probably damaging	Het
Slc4a10	A	G	2: 62,286,922	T712A	probably benign	Het
Sntb1	A	T	15: 55,792,072	Y249*	probably null	Het
Spatc1	A	G	15: 76,283,723	I127M	probably benign	Het
Synj1	A	T	16: 90,993,945	N109K	probably damaging	Het
Tdg	T	A	10: 82,648,641	M396K	possibly damaging	Het
Tmem173	C	A	18: 35,735,171	R292L	probably damaging	Het
Tmem252	T	C	19: 24,674,292	V75A	probably damaging	Het
Trim40	T	C	17: 36,883,976	Q142R	probably damaging	Het
Trip6	T	C	5: 137,312,966	T163A	probably damaging	Het
Ttn	T	C	2: 76,894,642		probably benign	Het
Wdr95	T	C	5: 149,611,540	L721P	probably benign	Het
Zfp385c	A	T	11: 100,630,687	D182E	probably damaging	Het
Zfp597	A	G	16: 3,865,767	F375S	probably benign	Het
Zfp658	T	G	7: 43,573,912	F537C	possibly damaging	Het
Zgrf1	T	C	3: 127,561,772	F216L	probably benign	Het

Other mutations in Prss35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01548:Prss35	APN	9	86755274	missense	probably benign	0.00
IGL02749:Prss35	APN	9	86756244	missense	probably damaging	1.00
R0346:Prss35	UTSW	9	86755351	missense	probably benign	0.04
R0403:Prss35	UTSW	9	86756037	missense	probably damaging	1.00
R1664:Prss35	UTSW	9	86755647	missense	probably benign	0.29
R2016:Prss35	UTSW	9	86755512	missense	probably benign	0.37
R2017:Prss35	UTSW	9	86755512	missense	probably benign	0.37
R2325:Prss35	UTSW	9	86756304	missense	probably damaging	1.00
R2429:Prss35	UTSW	9	86755345	missense	probably benign
R2965:Prss35	UTSW	9	86755582	missense	probably damaging	1.00
R2966:Prss35	UTSW	9	86755582	missense	probably damaging	1.00
R3961:Prss35	UTSW	9	86755749	missense	probably benign	0.02
R4792:Prss35	UTSW	9	86755669	missense	probably damaging	1.00
R4902:Prss35	UTSW	9	86756122	missense	probably damaging	1.00
R6169:Prss35	UTSW	9	86755438	missense	probably benign	0.00
R6446:Prss35	UTSW	9	86755653	missense	probably damaging	0.99
R6753:Prss35	UTSW	9	86756100	missense	probably damaging	1.00
R7387:Prss35	UTSW	9	86755921	missense	probably damaging	1.00
R7523:Prss35	UTSW	9	86755374	missense	probably damaging	1.00
R7587:Prss35	UTSW	9	86755374	missense	probably damaging	1.00
R7652:Prss35	UTSW	9	86755970	missense	probably benign
R8013:Prss35	UTSW	9	86755425	missense	probably damaging	1.00
R8194:Prss35	UTSW	9	86755613	missense	possibly damaging	0.94
R8871:Prss35	UTSW	9	86755191	missense	possibly damaging	0.84
R9444:Prss35	UTSW	9	86756104	missense	probably damaging	1.00
R9462:Prss35	UTSW	9	86756339	missense
R9695:Prss35	UTSW	9	86755708	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTCTGGATTTGACAACGACAG -3'
(R):5'- TGCATAGCTCATGAGTGGTC -3'

Sequencing Primer
(F):5'- CAGGGATGAACAGTTGGTGTATC -3'
(R):5'- CATAGCTCATGAGTGGTCTCTCAATG -3'

Posted On

2019-05-13