Incidental Mutation 'R7010:Zfp958'
ID544963
Institutional Source Beutler Lab
Gene Symbol Zfp958
Ensembl Gene ENSMUSG00000058748
Gene Namezinc finger protein 958
SynonymsBC003267
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.099) question?
Stock #R7010 (G1)
Quality Score225.009
Status Not validated
Chromosome8
Chromosomal Location4613167-4630231 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 4628377 bp
ZygosityHeterozygous
Amino Acid Change Isoleucine to Threonine at position 134 (I134T)
Ref Sequence ENSEMBL: ENSMUSP00000143842 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000073201] [ENSMUST00000202692]
Predicted Effect probably benign
Transcript: ENSMUST00000073201
AA Change: I133T

PolyPhen 2 Score 0.134 (Sensitivity: 0.92; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000072937
Gene: ENSMUSG00000058748
AA Change: I133T

DomainStartEndE-ValueType
KRAB 3 65 1.96e-17 SMART
ZnF_C2H2 102 124 2.67e-1 SMART
ZnF_C2H2 130 152 8.47e-4 SMART
ZnF_C2H2 158 180 5.5e-3 SMART
ZnF_C2H2 186 208 1.38e-3 SMART
ZnF_C2H2 214 236 9.96e-1 SMART
ZnF_C2H2 242 264 2.15e-5 SMART
ZnF_C2H2 270 292 3.16e-3 SMART
ZnF_C2H2 298 320 1.69e-3 SMART
ZnF_C2H2 326 348 5.14e-3 SMART
ZnF_C2H2 354 376 2.91e-2 SMART
ZnF_C2H2 382 404 2.27e-4 SMART
ZnF_C2H2 410 432 4.79e-3 SMART
ZnF_C2H2 438 460 6.88e-4 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000202692
AA Change: I134T

PolyPhen 2 Score 0.134 (Sensitivity: 0.92; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000143842
Gene: ENSMUSG00000058748
AA Change: I134T

DomainStartEndE-ValueType
KRAB 4 66 1.96e-17 SMART
ZnF_C2H2 103 125 2.67e-1 SMART
ZnF_C2H2 131 153 8.47e-4 SMART
ZnF_C2H2 159 181 5.5e-3 SMART
ZnF_C2H2 187 209 1.38e-3 SMART
ZnF_C2H2 215 237 9.96e-1 SMART
ZnF_C2H2 243 265 2.15e-5 SMART
ZnF_C2H2 271 293 3.16e-3 SMART
ZnF_C2H2 299 321 1.69e-3 SMART
ZnF_C2H2 327 349 5.14e-3 SMART
ZnF_C2H2 355 377 2.91e-2 SMART
ZnF_C2H2 383 405 2.27e-4 SMART
ZnF_C2H2 411 433 4.79e-3 SMART
ZnF_C2H2 439 461 6.88e-4 SMART
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.8%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 40 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
2210010C04Rik A C 6: 41,032,313 S196A probably benign Het
AL592187.3 A T 15: 77,602,597 Y58F probably benign Het
Ano10 T C 9: 122,253,124 T494A probably damaging Het
Asah2 A T 19: 32,054,554 F72I probably benign Het
Atat1 T C 17: 35,908,630 D114G probably damaging Het
Atp6v1e2 C T 17: 86,944,345 M208I probably benign Het
Bicd1 A G 6: 149,494,615 Y161C probably damaging Het
Camk2g T C 14: 20,741,444 S410G probably benign Het
Car2 C T 3: 14,900,053 P249L possibly damaging Het
Cdh23 T G 10: 60,530,991 I237L probably benign Het
Dlat T A 9: 50,657,974 K176N probably damaging Het
Dnajc12 T A 10: 63,397,280 C67S probably benign Het
Fat1 G T 8: 44,953,349 E1046* probably null Het
Gmip G A 8: 69,811,400 A137T probably damaging Het
Gpatch2l G A 12: 86,244,184 R47H probably damaging Het
Grk6 A G 13: 55,450,300 I62V possibly damaging Het
Hook1 G T 4: 96,014,811 L512F probably damaging Het
Ighe T C 12: 113,273,141 T36A Het
Il17rc A G 6: 113,479,288 N338S possibly damaging Het
Itgb6 T C 2: 60,649,978 Y338C probably damaging Het
Kcnd2 A G 6: 21,216,708 Y137C probably damaging Het
L3mbtl3 T A 10: 26,282,861 probably null Het
Lcn3 T C 2: 25,766,056 F41S probably damaging Het
Map3k8 T C 18: 4,334,060 H344R probably damaging Het
Marf1 A T 16: 14,137,001 I884N probably damaging Het
Nalcn G A 14: 123,293,465 T1387I probably damaging Het
Nrros T C 16: 32,143,580 T540A probably damaging Het
Olfr487 A T 7: 108,212,142 I129N probably damaging Het
Pank2 T C 2: 131,280,373 Y273H probably benign Het
Pgrmc2 A G 3: 41,082,633 V121A probably damaging Het
Phldb2 C T 16: 45,751,505 V1175M probably damaging Het
Ranbp2 A G 10: 58,454,571 probably null Het
Rsrc1 C T 3: 66,994,649 P44L unknown Het
Syt7 A G 19: 10,417,990 T55A probably benign Het
Tfcp2l1 T C 1: 118,653,727 S137P probably damaging Het
Tom1 T A 8: 75,051,975 V140D probably damaging Het
Ttc23l T G 15: 10,515,138 I385L probably damaging Het
Vmn1r122 A G 7: 21,133,971 V53A probably damaging Het
Vmn2r1 C T 3: 64,104,725 T669I probably benign Het
Vmn2r86 A G 10: 130,455,857 L13P probably benign Het
Other mutations in Zfp958
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01950:Zfp958 APN 8 4628917 missense probably damaging 1.00
IGL02185:Zfp958 APN 8 4628990 nonsense probably null
IGL02716:Zfp958 APN 8 4625967 critical splice donor site probably null
R1118:Zfp958 UTSW 8 4626169 missense possibly damaging 0.50
R1119:Zfp958 UTSW 8 4626169 missense possibly damaging 0.50
R1478:Zfp958 UTSW 8 4629190 missense probably damaging 1.00
R1816:Zfp958 UTSW 8 4629147 missense possibly damaging 0.89
R1837:Zfp958 UTSW 8 4628590 missense probably damaging 1.00
R1838:Zfp958 UTSW 8 4628590 missense probably damaging 1.00
R3763:Zfp958 UTSW 8 4626226 critical splice donor site probably null
R5257:Zfp958 UTSW 8 4628456 missense probably benign 0.28
R5258:Zfp958 UTSW 8 4628456 missense probably benign 0.28
R5304:Zfp958 UTSW 8 4626196 missense possibly damaging 0.73
R5512:Zfp958 UTSW 8 4625838 critical splice acceptor site probably null
R5907:Zfp958 UTSW 8 4629072 missense probably benign 0.01
R6604:Zfp958 UTSW 8 4628245 missense probably damaging 1.00
R6688:Zfp958 UTSW 8 4628940 missense possibly damaging 0.91
R6727:Zfp958 UTSW 8 4628247 missense probably benign 0.08
R6981:Zfp958 UTSW 8 4626170 missense probably benign 0.00
R7140:Zfp958 UTSW 8 4628481 missense probably benign 0.05
R8096:Zfp958 UTSW 8 4613273 start gained probably benign
R8491:Zfp958 UTSW 8 4626215 missense probably damaging 0.99
R8673:Zfp958 UTSW 8 4628268 missense possibly damaging 0.54
R8847:Zfp958 UTSW 8 4628434 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGCAACCCATATCAAAGGGATACAT -3'
(R):5'- TCATAAGGTTTCTCTCCAGTGTG -3'

Sequencing Primer
(F):5'- CTTTGCAGACCTGAAAGAAGTC -3'
(R):5'- GTGTTGTGAAAAGGCCTTATCAC -3'
Posted On2019-05-13