Mutagenetix > Incidental Mutation

Incidental Mutation 'R7013:Or4f15'

545111

Institutional Source

Beutler Lab

Gene Symbol

Or4f15

Ensembl Gene

ENSMUSG00000109528

Gene Name

olfactory receptor family 4 subfamily F member 15

Synonyms

MOR245-5, GA_x6K02T2Q125-73031456-73030518, Olfr1309

MMRRC Submission

045114-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.878) question?

Stock #

R7013 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

111813479-111814441 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 111814308 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Valine at position 37 (A37V)

Ref Sequence

ENSEMBL: ENSMUSP00000150871 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000207885] [ENSMUST00000214537] [ENSMUST00000214935] [ENSMUST00000215045] [ENSMUST00000216948] [ENSMUST00000217452]

AlphaFold

Q8VF83

Predicted Effect

probably benign
Transcript: ENSMUST00000207885
AA Change: A45V

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000214537
AA Change: A37V

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000214935
AA Change: A37V

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000215045
AA Change: A37V

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000216948
AA Change: A37V

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

Predicted Effect

probably benign
Transcript: ENSMUST00000217452
AA Change: A37V

PolyPhen 2 Score 0.307 (Sensitivity: 0.90; Specificity: 0.89)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (47/47)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	A	G	6: 121,618,345 (GRCm39)	I213V	probably null	Het
Abcc4	A	T	14: 118,763,755 (GRCm39)	C952S	probably benign	Het
Adhfe1	T	C	1: 9,620,816 (GRCm39)		probably benign	Het
Apob	T	A	12: 8,060,080 (GRCm39)	L2854*	probably null	Het
Arhgap5	T	A	12: 52,565,109 (GRCm39)	N693K	probably benign	Het
Arid5b	A	G	10: 67,933,649 (GRCm39)	V508A	probably damaging	Het
Atl1	A	G	12: 70,000,214 (GRCm39)	E288G	probably damaging	Het
Bank1	A	G	3: 135,806,270 (GRCm39)	S455P	possibly damaging	Het
Ces1c	A	T	8: 93,857,392 (GRCm39)	L63Q	probably damaging	Het
Crmp1	A	T	5: 37,426,036 (GRCm39)		probably null	Het
Csnka2ip	T	C	16: 64,298,780 (GRCm39)	D528G	unknown	Het
Dact2	T	C	17: 14,423,796 (GRCm39)	T66A	probably benign	Het
Dkk2	T	C	3: 131,880,760 (GRCm39)	L135P	probably damaging	Het
Drc3	A	G	11: 60,278,129 (GRCm39)	N381S	probably benign	Het
Dsg4	T	C	18: 20,591,578 (GRCm39)	V439A	possibly damaging	Het
Dysf	C	T	6: 84,114,340 (GRCm39)	P1240S	probably damaging	Het
Esyt1	A	T	10: 128,361,520 (GRCm39)	V58E	probably damaging	Het
Exo1	G	T	1: 175,721,338 (GRCm39)	A326S	probably damaging	Het
Fam20b	A	G	1: 156,518,135 (GRCm39)	S220P	probably damaging	Het
Fmo6	G	A	1: 162,745,817 (GRCm39)	T402I	probably benign	Het
Galnt10	A	T	11: 57,656,410 (GRCm39)	D198V	probably benign	Het
Gsap	A	T	5: 21,483,108 (GRCm39)	E604D	probably benign	Het
Il20rb	A	G	9: 100,343,481 (GRCm39)	Y258H	probably benign	Het
Impg1	A	T	9: 80,285,776 (GRCm39)	S409R	probably damaging	Het
Jak3	G	T	8: 72,131,425 (GRCm39)	V97F	possibly damaging	Het
Lbhd1	T	C	19: 8,861,523 (GRCm39)	S52P	probably damaging	Het
Lman2l	A	G	1: 36,482,599 (GRCm39)		probably benign	Het
Lnpep	C	A	17: 17,788,625 (GRCm39)	M493I	probably benign	Het
Mpp4	T	C	1: 59,188,774 (GRCm39)	D132G	probably damaging	Het
Nlrp1a	G	A	11: 71,014,378 (GRCm39)	R291W	probably benign	Het
Or1e23	G	T	11: 73,407,247 (GRCm39)	Y259*	probably null	Het
Or4a79	T	A	2: 89,551,730 (GRCm39)	I242F	probably benign	Het
Or4b13	C	T	2: 90,082,441 (GRCm39)	R297Q	possibly damaging	Het
Or4n4b	T	A	14: 50,536,656 (GRCm39)	I37F	probably damaging	Het
Orc5	A	G	5: 22,738,787 (GRCm39)	V158A	probably benign	Het
Pate8	C	A	9: 36,493,854 (GRCm39)	W26C	unknown	Het
Pcdhga3	A	G	18: 37,808,674 (GRCm39)	N376D	probably damaging	Het
Ptprr	T	A	10: 116,072,659 (GRCm39)	I207N	probably damaging	Het
Recql5	A	G	11: 115,785,402 (GRCm39)	V698A	probably benign	Het
Rnf212	A	T	5: 108,877,826 (GRCm39)	M222K	probably benign	Het
Rsph6a	A	G	7: 18,788,820 (GRCm39)	S51G	probably benign	Het
Smap2	T	C	4: 120,839,365 (GRCm39)	K121E	probably damaging	Het
Syt11	A	T	3: 88,655,296 (GRCm39)	V335D	possibly damaging	Het
Terb1	A	T	8: 105,215,222 (GRCm39)	C251*	probably null	Het
Tmprss11d	C	A	5: 86,474,432 (GRCm39)	R37L	probably damaging	Het
Ttbk2	T	C	2: 120,576,265 (GRCm39)	N904S	possibly damaging	Het
Vmn1r119	T	A	7: 20,745,714 (GRCm39)	I223F	probably damaging	Het
Vmn1r66	T	C	7: 10,008,683 (GRCm39)	R117G	possibly damaging	Het
Zfp106	T	C	2: 120,362,113 (GRCm39)	D1025G	probably damaging	Het

Other mutations in Or4f15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01160:Or4f15	APN	2	111,814,278 (GRCm39)	missense	probably damaging	1.00
IGL02479:Or4f15	APN	2	111,813,730 (GRCm39)	missense	probably benign	0.35
R0234:Or4f15	UTSW	2	111,813,645 (GRCm39)	missense	probably damaging	0.99
R0234:Or4f15	UTSW	2	111,813,645 (GRCm39)	missense	probably damaging	0.99
R1777:Or4f15	UTSW	2	111,814,042 (GRCm39)	missense	possibly damaging	0.64
R1994:Or4f15	UTSW	2	111,814,429 (GRCm39)	missense	probably benign
R3892:Or4f15	UTSW	2	111,813,486 (GRCm39)	missense	probably benign	0.03
R3946:Or4f15	UTSW	2	111,813,642 (GRCm39)	missense	possibly damaging	0.68
R4541:Or4f15	UTSW	2	111,813,981 (GRCm39)	missense	probably benign	0.01
R5150:Or4f15	UTSW	2	111,814,366 (GRCm39)	missense	probably benign	0.11
R5275:Or4f15	UTSW	2	111,814,174 (GRCm39)	missense	probably damaging	1.00
R5293:Or4f15	UTSW	2	111,813,611 (GRCm39)	missense	probably damaging	0.99
R6080:Or4f15	UTSW	2	111,814,050 (GRCm39)	missense	probably damaging	1.00
R6258:Or4f15	UTSW	2	111,814,396 (GRCm39)	missense	probably benign	0.05
R6260:Or4f15	UTSW	2	111,814,396 (GRCm39)	missense	probably benign	0.05
R6291:Or4f15	UTSW	2	111,813,969 (GRCm39)	missense	probably benign	0.00
R6442:Or4f15	UTSW	2	111,813,874 (GRCm39)	missense	probably damaging	0.99
R7326:Or4f15	UTSW	2	111,813,672 (GRCm39)	nonsense	probably null
R7483:Or4f15	UTSW	2	111,814,124 (GRCm39)	missense	probably damaging	0.98
R8078:Or4f15	UTSW	2	111,813,615 (GRCm39)	missense	probably damaging	1.00
R9055:Or4f15	UTSW	2	111,814,049 (GRCm39)	nonsense	probably null
R9800:Or4f15	UTSW	2	111,814,194 (GRCm39)	missense	possibly damaging	0.91
X0062:Or4f15	UTSW	2	111,813,946 (GRCm39)	missense	probably benign	0.00
Z1176:Or4f15	UTSW	2	111,814,098 (GRCm39)	missense	probably benign	0.10

Predicted Primers

PCR Primer
(F):5'- CCCCAGAAGGAGATGGTTTTG -3'
(R):5'- ATTACCTCCACAGTTGCAGG -3'

Sequencing Primer
(F):5'- GTGTTTCTTGAAAATGTCACAGATC -3'
(R):5'- GCAGGCTTTCCTTTCTAAACACTAAC -3'

Posted On

2019-05-13