Mutagenetix > Incidental Mutation

Incidental Mutation 'R7014:Gstm7'

545165

Institutional Source

Beutler Lab

Gene Symbol

Gstm7

Ensembl Gene

ENSMUSG00000004035

Gene Name

glutathione S-transferase, mu 7

Synonyms

Cd203c, 0610005A07Rik

MMRRC Submission

045115-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.218) question?

Stock #

R7014 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

107833650-107839133 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 107834278 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Asparagine at position 196 (D196N)

Ref Sequence

ENSEMBL: ENSMUSP00000004137 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000004137] [ENSMUST00000106687] [ENSMUST00000106688] [ENSMUST00000124215] [ENSMUST00000133947]

AlphaFold

Q80W21

PDB Structure

Crystal structure of mouse glutathione S-transferase, mu7 (GSTM7) at 1.6 A resolution [X-RAY DIFFRACTION]

Predicted Effect

probably benign
Transcript: ENSMUST00000004137
AA Change: D196N

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000004137
Gene: ENSMUSG00000004035
AA Change: D196N

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	2.2e-23	PFAM
Pfam:GST_C_3	42	190	1.2e-9	PFAM
Pfam:GST_C	104	191	5.3e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000106687
AA Change: D159N

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000102298
Gene: ENSMUSG00000004035
AA Change: D159N

Domain	Start	End	E-Value	Type
Pfam:GST_N	3	82	6.8e-24	PFAM
Pfam:GST_N_3	11	93	1.1e-6	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000106688
AA Change: D192N

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000102299
Gene: ENSMUSG00000004035
AA Change: D192N

Domain	Start	End	E-Value	Type
Pfam:GST_N_3	4	89	8.8e-7	PFAM
Pfam:GST_N	5	78	4.2e-19	PFAM
Pfam:GST_C	100	188	5.6e-16	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000124215

SMART Domains

Protein: ENSMUSP00000118707
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
Pfam:GST_N	1	72	8.6e-20	PFAM
Pfam:GST_N_3	3	83	4e-7	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000133947

SMART Domains

Protein: ENSMUSP00000122567
Gene: ENSMUSG00000004035

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:GST_N	45	123	2.6e-19	PFAM
Pfam:GST_N_3	54	134	1.4e-6	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 80 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022A10Rik	C	T	7: 27,278,198 (GRCm39)	Q156*	probably null	Het
2700097O09Rik	A	G	12: 55,092,727 (GRCm39)	I264T	probably benign	Het
Arid3a	G	T	10: 79,786,718 (GRCm39)	M488I	possibly damaging	Het
Auts2	A	G	5: 131,494,961 (GRCm39)	F331S	probably damaging	Het
Axdnd1	T	C	1: 156,158,532 (GRCm39)		probably null	Het
Bpifb5	C	A	2: 154,066,876 (GRCm39)	S43*	probably null	Het
Carhsp1	A	G	16: 8,478,869 (GRCm39)	V128A	probably benign	Het
Ccdc141	A	G	2: 76,962,641 (GRCm39)	V101A	probably damaging	Het
Cdk6	C	G	5: 3,523,152 (GRCm39)	L191V	probably damaging	Het
Cgnl1	T	G	9: 71,632,416 (GRCm39)	K312Q	possibly damaging	Het
Col6a1	T	C	10: 76,557,277 (GRCm39)	E225G	probably damaging	Het
Copg1	T	A	6: 87,879,322 (GRCm39)	L456Q	probably damaging	Het
Ctif	T	A	18: 75,570,279 (GRCm39)	D540V	possibly damaging	Het
Cyfip1	T	C	7: 55,569,241 (GRCm39)	I917T	probably benign	Het
Cyth1	T	C	11: 118,103,477 (GRCm39)	D9G	probably benign	Het
Dnajb1	T	C	8: 84,336,884 (GRCm39)	I118T	probably damaging	Het
Ebna1bp2	T	A	4: 118,480,575 (GRCm39)	Y139*	probably null	Het
Efcab3	A	G	11: 104,584,248 (GRCm39)	T100A	probably benign	Het
Fam162a	A	T	16: 35,870,302 (GRCm39)	V59E	probably damaging	Het
Fhad1	CGG	CG	4: 141,645,602 (GRCm39)		probably null	Het
Fmo6	C	G	1: 162,753,877 (GRCm39)	R112T	probably benign	Het
Gm17087	T	A	17: 8,785,304 (GRCm39)	D133V	probably benign	Het
Gm42669	A	G	5: 107,656,142 (GRCm39)	I802V	probably benign	Het
Gp2	T	C	7: 119,050,868 (GRCm39)	N288D	probably damaging	Het
Hsph1	A	T	5: 149,553,865 (GRCm39)	V201D	probably damaging	Het
Il20ra	T	C	10: 19,588,458 (GRCm39)	L26P	unknown	Het
Itpr1	T	A	6: 108,408,459 (GRCm39)		probably null	Het
Kcns3	A	T	12: 11,141,688 (GRCm39)	I337N	probably damaging	Het
Kdm4d	A	G	9: 14,375,475 (GRCm39)	Y128H	probably damaging	Het
Kirrel2	A	G	7: 30,153,999 (GRCm39)	I200T	probably benign	Het
Klhdc10	T	A	6: 30,450,502 (GRCm39)	I294N	probably damaging	Het
Map1a	A	C	2: 121,130,720 (GRCm39)	N512T	probably damaging	Het
Marchf8	C	G	6: 116,380,504 (GRCm39)	C118W	probably damaging	Het
Marchf8	T	G	6: 116,380,505 (GRCm39)	C119G	probably damaging	Het
Mrpl38	G	T	11: 116,025,741 (GRCm39)	P195Q	probably damaging	Het
Ms4a4d	A	T	19: 11,525,947 (GRCm39)	Q27L	probably benign	Het
Muc16	T	A	9: 18,569,532 (GRCm39)	T996S	unknown	Het
Mug1	C	T	6: 121,838,084 (GRCm39)	A438V	probably benign	Het
Mup7	T	A	4: 60,069,866 (GRCm39)	I33F	probably damaging	Het
Ndufa5	C	T	6: 24,519,190 (GRCm39)		probably null	Het
Or2b7	A	G	13: 21,740,108 (GRCm39)	F28S	probably benign	Het
Or2l5	A	T	16: 19,334,206 (GRCm39)	F60Y	probably benign	Het
Or5w17	T	C	2: 87,584,215 (GRCm39)	I41V	probably benign	Het
Or5w1b	A	T	2: 87,476,320 (GRCm39)	L49Q	probably damaging	Het
Or7e177	T	A	9: 20,211,959 (GRCm39)	C154*	probably null	Het
Parp9	T	A	16: 35,780,433 (GRCm39)		probably null	Het
Pate8	C	A	9: 36,493,854 (GRCm39)	W26C	unknown	Het
Pbx1	T	A	1: 168,258,949 (GRCm39)	D42V	probably damaging	Het
Pde4dip	G	T	3: 97,622,738 (GRCm39)	N1490K	possibly damaging	Het
Pdzd2	T	C	15: 12,372,647 (GRCm39)	N2496S	probably benign	Het
Pdzd2	A	G	15: 12,373,061 (GRCm39)	M2358T	probably benign	Het
Pglyrp2	C	A	17: 32,634,904 (GRCm39)	C486F	probably damaging	Het
Pi4ka	A	G	16: 17,114,931 (GRCm39)		probably benign	Het
Pknox2	T	A	9: 36,820,963 (GRCm39)	T300S	probably damaging	Het
Plekhm3	A	G	1: 64,922,429 (GRCm39)	I582T	probably damaging	Het
Prtg	T	C	9: 72,799,267 (GRCm39)	Y766H	possibly damaging	Het
Prxl2a	T	C	14: 40,724,451 (GRCm39)	E71G	probably benign	Het
Rabgap1	A	G	2: 37,450,575 (GRCm39)	E901G	probably benign	Het
Rabgap1l	A	T	1: 160,169,642 (GRCm39)	N60K	probably damaging	Het
Rnf150	C	T	8: 83,769,292 (GRCm39)	T359I	probably benign	Het
Rps6ka2	C	T	17: 7,523,331 (GRCm39)	H236Y	probably benign	Het
Rrp1b	T	A	17: 32,268,401 (GRCm39)	L120Q	probably damaging	Het
Scn7a	C	A	2: 66,572,303 (GRCm39)	G223C	probably null	Het
Sema6a	T	A	18: 47,431,284 (GRCm39)	N138I	probably damaging	Het
Septin5	T	A	16: 18,443,659 (GRCm39)	I97F	probably damaging	Het
Shcbp1	T	A	8: 4,804,234 (GRCm39)	E225D	probably damaging	Het
Slc25a19	A	G	11: 115,511,792 (GRCm39)	C124R	probably damaging	Het
Slc37a2	C	T	9: 37,145,183 (GRCm39)	A428T	probably damaging	Het
Slc5a12	A	G	2: 110,474,709 (GRCm39)	I538V	probably benign	Het
Slco4c1	A	T	1: 96,751,506 (GRCm39)		probably null	Het
Smarcad1	T	C	6: 65,029,654 (GRCm39)	S81P	probably damaging	Het
Smyd1	T	C	6: 71,215,611 (GRCm39)	D116G	probably damaging	Het
Themis	T	C	10: 28,665,703 (GRCm39)	Y589H	probably benign	Het
Trim30d	T	C	7: 104,132,543 (GRCm39)	K248R	probably benign	Het
Tspan12	C	T	6: 21,772,918 (GRCm39)	M210I	probably benign	Het
Vmn2r11	T	C	5: 109,201,289 (GRCm39)	Y405C	probably damaging	Het
Wdfy3	A	G	5: 102,042,775 (GRCm39)		probably null	Het
Wiz	C	A	17: 32,580,840 (GRCm39)	A83S	probably damaging	Het
Zfp1002	A	T	2: 150,097,182 (GRCm39)	C82*	probably null	Het
Zmym6	T	A	4: 127,017,337 (GRCm39)	Y947*	probably null	Het

Other mutations in Gstm7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02215:Gstm7	APN	3	107,837,594 (GRCm39)	missense	possibly damaging	0.93
PIT4142001:Gstm7	UTSW	3	107,838,799 (GRCm39)	frame shift	probably null
R0095:Gstm7	UTSW	3	107,837,879 (GRCm39)	splice site	probably benign
R0961:Gstm7	UTSW	3	107,834,302 (GRCm39)	unclassified	probably benign
R1052:Gstm7	UTSW	3	107,834,266 (GRCm39)	missense	probably benign	0.05
R2121:Gstm7	UTSW	3	107,834,230 (GRCm39)	missense	probably benign	0.00
R4610:Gstm7	UTSW	3	107,834,235 (GRCm39)	missense	possibly damaging	0.65
R5966:Gstm7	UTSW	3	107,838,747 (GRCm39)	intron	probably benign
R6393:Gstm7	UTSW	3	107,838,142 (GRCm39)	critical splice donor site	probably null
R7052:Gstm7	UTSW	3	107,838,633 (GRCm39)	missense	probably damaging	0.96
R7741:Gstm7	UTSW	3	107,838,963 (GRCm39)	missense	possibly damaging	0.90
R7848:Gstm7	UTSW	3	107,835,902 (GRCm39)	splice site	probably null
R7988:Gstm7	UTSW	3	107,834,271 (GRCm39)	missense	possibly damaging	0.82
R7998:Gstm7	UTSW	3	107,837,657 (GRCm39)	missense	probably damaging	1.00
R8952:Gstm7	UTSW	3	107,838,757 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- TGAGGCTTGGGATCTGGAAC -3'
(R):5'- GTCATAATCTATAATGGGCACGATCC -3'

Sequencing Primer
(F):5'- AAGAAGGAAAAGTTGGCTGTGG -3'
(R):5'- ACGATCCCGATGCACAGG -3'

Posted On

2019-05-13