Mutagenetix > Incidental Mutation

Incidental Mutation 'R7022:Lama5'

545675

Institutional Source

Beutler Lab

Gene Symbol

Lama5

Ensembl Gene

ENSMUSG00000015647

Gene Name

laminin, alpha 5

Synonyms

MMRRC Submission

045123-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7022 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

179818166-179867652 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 179822524 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Leucine at position 2850 (V2850L)

Ref Sequence

ENSEMBL: ENSMUSP00000015791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015791] [ENSMUST00000061437]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA5 CHAIN N-TERMINAL FRAGMENT [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000015791
AA Change: V2850L

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647
AA Change: V2850L

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000061437

SMART Domains

Protein: ENSMUSP00000050076
Gene: ENSMUSG00000039041

Domain	Start	End	E-Value	Type
Pfam:Proteasom_Rpn13	29	111	3.5e-35	PFAM
low complexity region	135	161	N/A	INTRINSIC
low complexity region	173	254	N/A	INTRINSIC
Pfam:RPN13_C	268	381	7.3e-38	PFAM
low complexity region	390	403	N/A	INTRINSIC

Meta Mutation Damage Score

0.1243

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (75/75)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit disrupted basal laminae leading to exencephaly, syndactyly, placentopathy, kidney defects, abnormal lobar septation with absence of a visceral pleural membrane, and lethality in late gestation. [provided by MGI curators]

Allele List at MGI

All alleles(49) : Targeted(5) Gene trapped(44)

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	A	T	11: 109,974,326 (GRCm39)	S203T	probably damaging	Het
Adat1	A	T	8: 112,716,494 (GRCm39)	M76K	probably damaging	Het
Ankrd33	G	C	15: 101,014,780 (GRCm39)	V56L	probably benign	Het
Ascc3	T	A	10: 50,592,725 (GRCm39)	L1134I	possibly damaging	Het
C3	T	G	17: 57,524,286 (GRCm39)	D948A	probably damaging	Het
Ccdc121rt3	A	C	5: 112,503,395 (GRCm39)	M103R	probably benign	Het
Ccdc40	A	G	11: 119,122,612 (GRCm39)	E138G	possibly damaging	Het
Cd80	A	G	16: 38,306,866 (GRCm39)		probably null	Het
Cdca7	T	A	2: 72,309,873 (GRCm39)		probably null	Het
Chit1	A	G	1: 134,079,030 (GRCm39)	S447G	probably benign	Het
Cmya5	C	T	13: 93,205,786 (GRCm39)		probably null	Het
Cnnm2	A	T	19: 46,750,989 (GRCm39)	I260F	probably damaging	Het
Cnnm2	T	A	19: 46,847,379 (GRCm39)		probably null	Het
Col1a2	T	C	6: 4,534,639 (GRCm39)	L881P	unknown	Het
Crebbp	A	T	16: 3,935,187 (GRCm39)	S901T	probably damaging	Het
Dcun1d2	T	C	8: 13,321,637 (GRCm39)	Y158C	probably damaging	Het
Dnhd1	C	T	7: 105,370,005 (GRCm39)	P4477S	probably benign	Het
Donson	A	G	16: 91,478,218 (GRCm39)	Y428H	probably damaging	Het
Dsp	G	T	13: 38,375,716 (GRCm39)	W1167L	probably benign	Het
Elmo2	C	T	2: 165,136,961 (GRCm39)	V592M	probably damaging	Het
Enpp3	T	A	10: 24,702,093 (GRCm39)	E60D	probably damaging	Het
Erfl	A	T	7: 24,631,089 (GRCm39)		probably null	Het
Fam83b	TAAGA	T	9: 76,409,394 (GRCm39)		probably null	Het
Fbxo38	A	G	18: 62,669,295 (GRCm39)	C52R	probably damaging	Het
Gm10912	T	C	2: 103,897,055 (GRCm39)	W65R	probably damaging	Het
Gm43518	A	T	5: 124,074,490 (GRCm39)	M44L	probably benign	Het
Gnb1	T	A	4: 155,637,913 (GRCm39)	D212E	probably damaging	Het
Gnl1	A	G	17: 36,299,620 (GRCm39)	N597S	probably damaging	Het
Gprin1	T	G	13: 54,886,855 (GRCm39)	E473A	probably benign	Het
Ighv1-69	T	A	12: 115,586,834 (GRCm39)	M100L	probably benign	Het
Igkv14-100	T	C	6: 68,496,193 (GRCm39)	S29P	probably damaging	Het
Ints6	A	G	14: 62,951,786 (GRCm39)	V232A	probably damaging	Het
Jup	A	G	11: 100,270,379 (GRCm39)	L376P	probably damaging	Het
Kdr	A	T	5: 76,132,920 (GRCm39)	Y119*	probably null	Het
Kif1a	T	G	1: 92,993,820 (GRCm39)	T377P	probably benign	Het
Lrp2	T	C	2: 69,313,552 (GRCm39)	Y2393C	probably damaging	Het
Mfsd13a	C	T	19: 46,356,763 (GRCm39)	Q255*	probably null	Het
Msh3	A	C	13: 92,372,096 (GRCm39)	D891E	probably damaging	Het
Msl2	A	G	9: 100,957,335 (GRCm39)	N2D	possibly damaging	Het
Myo19	T	C	11: 84,791,373 (GRCm39)	L476P	probably damaging	Het
Nbeal2	G	A	9: 110,467,686 (GRCm39)	R501W	probably damaging	Het
Nfasc	C	A	1: 132,548,787 (GRCm39)	K293N	probably damaging	Het
Npbwr1	A	T	1: 5,987,319 (GRCm39)	L65Q	probably damaging	Het
Nrbp1	T	A	5: 31,401,825 (GRCm39)	D34E	probably damaging	Het
Nup205	T	A	6: 35,220,871 (GRCm39)	V1891D	probably benign	Het
Or51g2	T	A	7: 102,623,175 (GRCm39)	N8I	possibly damaging	Het
Or5g29	T	A	2: 85,420,942 (GRCm39)	D19E	probably benign	Het
Or5k1b	T	C	16: 58,581,482 (GRCm39)	N19S	probably benign	Het
Or8g22	G	T	9: 38,958,379 (GRCm39)	C156*	probably null	Het
Oscp1	G	A	4: 125,976,783 (GRCm39)		probably null	Het
Pcdhgb7	A	G	18: 37,886,086 (GRCm39)	T419A	probably damaging	Het
Pdzph1	T	C	17: 59,281,121 (GRCm39)	D387G	probably benign	Het
Phc1	A	G	6: 122,311,990 (GRCm39)	F56S	probably damaging	Het
Pik3c2g	A	G	6: 139,599,061 (GRCm39)	E59G	possibly damaging	Het
Prickle1	G	T	15: 93,398,752 (GRCm39)	T692K	possibly damaging	Het
Rbm45	T	A	2: 76,206,738 (GRCm39)	L250Q	probably damaging	Het
Ror1	T	A	4: 100,265,108 (GRCm39)	M194K	probably damaging	Het
Rrbp1	T	A	2: 143,799,722 (GRCm39)		probably null	Het
Rundc3b	T	C	5: 8,562,348 (GRCm39)	K340R	probably null	Het
Scn1a	T	A	2: 66,148,243 (GRCm39)	T1101S	probably damaging	Het
Sdk1	C	G	5: 142,080,412 (GRCm39)		probably null	Het
Skic2	A	G	17: 35,064,183 (GRCm39)	F501S	possibly damaging	Het
Slc20a1	T	A	2: 129,041,979 (GRCm39)	M114K	probably damaging	Het
Sorl1	A	C	9: 41,881,047 (GRCm39)	I2158S	probably benign	Het
Strip1	A	T	3: 107,534,111 (GRCm39)	F174L	probably benign	Het
Sucnr1	A	T	3: 59,993,699 (GRCm39)	I76L	probably benign	Het
Syt17	C	T	7: 118,007,242 (GRCm39)	V412I	probably benign	Het
Tie1	T	A	4: 118,346,850 (GRCm39)	H18L	probably benign	Het
Tpp1	T	G	7: 105,398,129 (GRCm39)	K345Q	probably damaging	Het
Trcg1	G	A	9: 57,148,852 (GRCm39)	M141I	possibly damaging	Het
Trpc4ap	T	C	2: 155,499,742 (GRCm39)	N260S	probably benign	Het
Vps41	C	A	13: 19,026,438 (GRCm39)	T512K	possibly damaging	Het
Vps52	A	T	17: 34,178,293 (GRCm39)	M147L	probably benign	Het
Ywhaq	A	G	12: 21,441,752 (GRCm39)		probably benign	Het
Zfp619	A	G	7: 39,184,387 (GRCm39)	N139S	probably benign	Het

Other mutations in Lama5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Lama5	APN	2	179,818,336 (GRCm39)	unclassified	probably benign
IGL01370:Lama5	APN	2	179,839,193 (GRCm39)	missense	possibly damaging	0.87
IGL01474:Lama5	APN	2	179,838,363 (GRCm39)	missense	probably damaging	1.00
IGL01614:Lama5	APN	2	179,822,657 (GRCm39)	missense	probably damaging	1.00
IGL01941:Lama5	APN	2	179,834,185 (GRCm39)	missense	possibly damaging	0.71
IGL01953:Lama5	APN	2	179,832,497 (GRCm39)	missense	probably damaging	0.97
IGL02093:Lama5	APN	2	179,830,380 (GRCm39)	missense	probably damaging	1.00
IGL02197:Lama5	APN	2	179,849,012 (GRCm39)	missense	possibly damaging	0.82
IGL02308:Lama5	APN	2	179,832,120 (GRCm39)	splice site	probably benign
IGL02314:Lama5	APN	2	179,836,275 (GRCm39)	splice site	probably benign
IGL02317:Lama5	APN	2	179,833,112 (GRCm39)	missense	probably damaging	1.00
IGL02354:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02361:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02557:Lama5	APN	2	179,832,725 (GRCm39)	nonsense	probably null
IGL03026:Lama5	APN	2	179,837,760 (GRCm39)	missense	probably benign	0.34
IGL03160:Lama5	APN	2	179,822,128 (GRCm39)	missense	probably damaging	1.00
IGL03238:Lama5	APN	2	179,830,367 (GRCm39)	missense	probably benign
IGL03390:Lama5	APN	2	179,849,011 (GRCm39)	missense	probably damaging	1.00
blancmange	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
cupcake	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
layercake	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
poundcake	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
Salty	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
PIT4378001:Lama5	UTSW	2	179,831,238 (GRCm39)	missense	possibly damaging	0.89
R0003:Lama5	UTSW	2	179,819,872 (GRCm39)	splice site	probably null
R0056:Lama5	UTSW	2	179,828,899 (GRCm39)	intron	probably benign
R0147:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0148:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0310:Lama5	UTSW	2	179,823,359 (GRCm39)	splice site	probably benign
R0326:Lama5	UTSW	2	179,824,219 (GRCm39)	missense	possibly damaging	0.90
R0368:Lama5	UTSW	2	179,823,023 (GRCm39)	nonsense	probably null
R0479:Lama5	UTSW	2	179,826,250 (GRCm39)	missense	probably benign	0.03
R0490:Lama5	UTSW	2	179,821,962 (GRCm39)	missense	possibly damaging	0.90
R0636:Lama5	UTSW	2	179,831,124 (GRCm39)	critical splice donor site	probably null
R0704:Lama5	UTSW	2	179,821,277 (GRCm39)	missense	possibly damaging	0.84
R0733:Lama5	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
R1017:Lama5	UTSW	2	179,837,213 (GRCm39)	missense	probably damaging	1.00
R1078:Lama5	UTSW	2	179,821,557 (GRCm39)	unclassified	probably benign
R1294:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1423:Lama5	UTSW	2	179,837,434 (GRCm39)	missense	probably damaging	1.00
R1438:Lama5	UTSW	2	179,824,593 (GRCm39)	missense	probably benign	0.01
R1447:Lama5	UTSW	2	179,827,671 (GRCm39)	missense	probably damaging	0.99
R1540:Lama5	UTSW	2	179,821,944 (GRCm39)	missense	probably benign
R1601:Lama5	UTSW	2	179,839,538 (GRCm39)	missense	probably damaging	1.00
R1624:Lama5	UTSW	2	179,848,551 (GRCm39)	missense	probably benign	0.02
R1674:Lama5	UTSW	2	179,843,780 (GRCm39)	missense	probably benign	0.00
R1687:Lama5	UTSW	2	179,835,859 (GRCm39)	missense	probably benign	0.00
R1696:Lama5	UTSW	2	179,844,279 (GRCm39)	missense	probably damaging	1.00
R1701:Lama5	UTSW	2	179,863,162 (GRCm39)	missense	probably damaging	1.00
R1778:Lama5	UTSW	2	179,837,274 (GRCm39)	splice site	probably benign
R1936:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1939:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1940:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1953:Lama5	UTSW	2	179,832,540 (GRCm39)	missense	possibly damaging	0.94
R1966:Lama5	UTSW	2	179,830,145 (GRCm39)	missense	probably damaging	1.00
R2024:Lama5	UTSW	2	179,820,923 (GRCm39)	missense	probably benign	0.00
R2079:Lama5	UTSW	2	179,867,301 (GRCm39)	missense	possibly damaging	0.68
R2115:Lama5	UTSW	2	179,828,678 (GRCm39)	missense	probably damaging	1.00
R2173:Lama5	UTSW	2	179,838,035 (GRCm39)	missense	probably benign	0.00
R2272:Lama5	UTSW	2	179,820,396 (GRCm39)	missense	possibly damaging	0.93
R2357:Lama5	UTSW	2	179,821,890 (GRCm39)	missense	probably benign	0.01
R2860:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2861:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2939:Lama5	UTSW	2	179,840,747 (GRCm39)	missense	probably damaging	1.00
R3053:Lama5	UTSW	2	179,824,860 (GRCm39)	missense	probably damaging	0.99
R3430:Lama5	UTSW	2	179,838,110 (GRCm39)	missense	probably benign	0.00
R3752:Lama5	UTSW	2	179,829,015 (GRCm39)	missense	probably damaging	1.00
R3782:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	possibly damaging	0.57
R3901:Lama5	UTSW	2	179,824,144 (GRCm39)	splice site	probably benign
R4248:Lama5	UTSW	2	179,822,220 (GRCm39)	missense	possibly damaging	0.84
R4626:Lama5	UTSW	2	179,826,253 (GRCm39)	missense	probably damaging	0.98
R4638:Lama5	UTSW	2	179,832,206 (GRCm39)	missense	possibly damaging	0.89
R4669:Lama5	UTSW	2	179,822,430 (GRCm39)	missense	probably damaging	1.00
R4673:Lama5	UTSW	2	179,841,059 (GRCm39)	missense	probably damaging	1.00
R4677:Lama5	UTSW	2	179,821,159 (GRCm39)	missense	possibly damaging	0.69
R4701:Lama5	UTSW	2	179,833,489 (GRCm39)	missense	probably damaging	1.00
R4774:Lama5	UTSW	2	179,827,734 (GRCm39)	missense	probably damaging	1.00
R4880:Lama5	UTSW	2	179,818,861 (GRCm39)	unclassified	probably benign
R4923:Lama5	UTSW	2	179,825,942 (GRCm39)	missense	probably benign	0.18
R4960:Lama5	UTSW	2	179,850,045 (GRCm39)	critical splice donor site	probably null
R4983:Lama5	UTSW	2	179,835,242 (GRCm39)	missense	probably benign	0.13
R5061:Lama5	UTSW	2	179,840,579 (GRCm39)	nonsense	probably null
R5080:Lama5	UTSW	2	179,848,993 (GRCm39)	nonsense	probably null
R5135:Lama5	UTSW	2	179,844,013 (GRCm39)	missense	possibly damaging	0.89
R5206:Lama5	UTSW	2	179,833,097 (GRCm39)	missense	probably damaging	1.00
R5296:Lama5	UTSW	2	179,835,594 (GRCm39)	missense	probably damaging	1.00
R5319:Lama5	UTSW	2	179,822,911 (GRCm39)	missense	probably damaging	1.00
R5355:Lama5	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
R5388:Lama5	UTSW	2	179,832,539 (GRCm39)	missense	possibly damaging	0.83
R5528:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	probably benign	0.21
R5536:Lama5	UTSW	2	179,831,142 (GRCm39)	missense	probably damaging	0.99
R5658:Lama5	UTSW	2	179,850,069 (GRCm39)	nonsense	probably null
R5823:Lama5	UTSW	2	179,834,285 (GRCm39)	missense	probably benign	0.04
R5885:Lama5	UTSW	2	179,843,624 (GRCm39)	missense	probably damaging	1.00
R5889:Lama5	UTSW	2	179,835,467 (GRCm39)	intron	probably benign
R5912:Lama5	UTSW	2	179,837,268 (GRCm39)	missense	probably damaging	1.00
R5955:Lama5	UTSW	2	179,839,267 (GRCm39)	missense	probably damaging	1.00
R6015:Lama5	UTSW	2	179,827,185 (GRCm39)	missense	probably benign	0.36
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
R6359:Lama5	UTSW	2	179,837,775 (GRCm39)	missense	probably benign	0.01
R6385:Lama5	UTSW	2	179,838,326 (GRCm39)	missense	probably damaging	1.00
R6406:Lama5	UTSW	2	179,839,257 (GRCm39)	nonsense	probably null
R6552:Lama5	UTSW	2	179,822,947 (GRCm39)	missense	probably damaging	0.98
R6632:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6633:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6645:Lama5	UTSW	2	179,821,463 (GRCm39)	missense	probably damaging	1.00
R6731:Lama5	UTSW	2	179,830,367 (GRCm39)	missense	probably benign	0.09
R6744:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6798:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6799:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6801:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6851:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6869:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6881:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6882:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6884:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R7204:Lama5	UTSW	2	179,843,970 (GRCm39)	missense	probably damaging	1.00
R7207:Lama5	UTSW	2	179,848,877 (GRCm39)	missense	probably damaging	0.98
R7282:Lama5	UTSW	2	179,843,588 (GRCm39)	missense	probably damaging	1.00
R7367:Lama5	UTSW	2	179,834,751 (GRCm39)	missense	probably benign	0.01
R7410:Lama5	UTSW	2	179,844,183 (GRCm39)	critical splice donor site	probably null
R7699:Lama5	UTSW	2	179,822,654 (GRCm39)	missense	probably damaging	1.00
R7849:Lama5	UTSW	2	179,843,605 (GRCm39)	missense	probably damaging	1.00
R7909:Lama5	UTSW	2	179,834,069 (GRCm39)	missense	possibly damaging	0.95
R7948:Lama5	UTSW	2	179,843,994 (GRCm39)	missense	probably damaging	1.00
R8153:Lama5	UTSW	2	179,829,724 (GRCm39)	missense	probably benign	0.37
R8317:Lama5	UTSW	2	179,848,784 (GRCm39)	missense	probably damaging	1.00
R8351:Lama5	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
R8370:Lama5	UTSW	2	179,843,280 (GRCm39)	missense	possibly damaging	0.80
R8398:Lama5	UTSW	2	179,838,827 (GRCm39)	critical splice donor site	probably null
R8401:Lama5	UTSW	2	179,840,580 (GRCm39)	missense	probably damaging	1.00
R8404:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8502:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8694:Lama5	UTSW	2	179,822,677 (GRCm39)	missense	probably damaging	0.98
R8705:Lama5	UTSW	2	179,820,354 (GRCm39)	missense	probably damaging	1.00
R8732:Lama5	UTSW	2	179,828,481 (GRCm39)	missense	probably damaging	1.00
R8755:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R8786:Lama5	UTSW	2	179,838,100 (GRCm39)	missense	probably damaging	1.00
R8926:Lama5	UTSW	2	179,835,783 (GRCm39)	missense	probably benign	0.08
R8928:Lama5	UTSW	2	179,843,832 (GRCm39)	missense	probably damaging	1.00
R8953:Lama5	UTSW	2	179,835,313 (GRCm39)	missense	probably damaging	0.99
R8958:Lama5	UTSW	2	179,835,592 (GRCm39)	missense	probably benign
R9002:Lama5	UTSW	2	179,838,311 (GRCm39)	missense	probably damaging	1.00
R9081:Lama5	UTSW	2	179,833,930 (GRCm39)	nonsense	probably null
R9165:Lama5	UTSW	2	179,821,286 (GRCm39)	missense	probably damaging	0.99
R9233:Lama5	UTSW	2	179,840,502 (GRCm39)	nonsense	probably null
R9264:Lama5	UTSW	2	179,838,271 (GRCm39)	splice site	probably benign
R9311:Lama5	UTSW	2	179,838,275 (GRCm39)	critical splice donor site	probably null
R9443:Lama5	UTSW	2	179,843,522 (GRCm39)	missense	probably benign	0.00
R9488:Lama5	UTSW	2	179,823,234 (GRCm39)	missense	possibly damaging	0.95
R9674:Lama5	UTSW	2	179,840,267 (GRCm39)	critical splice donor site	probably null
R9684:Lama5	UTSW	2	179,849,038 (GRCm39)	missense	probably damaging	1.00
R9749:Lama5	UTSW	2	179,825,433 (GRCm39)	missense	probably benign	0.00
RF020:Lama5	UTSW	2	179,837,971 (GRCm39)	missense	probably benign
X0065:Lama5	UTSW	2	179,823,524 (GRCm39)	missense	probably benign	0.26
Z1177:Lama5	UTSW	2	179,832,507 (GRCm39)	missense	possibly damaging	0.95
Z1177:Lama5	UTSW	2	179,831,212 (GRCm39)	missense	probably damaging	1.00
Z1177:Lama5	UTSW	2	179,825,423 (GRCm39)	missense	probably benign	0.03
Z1177:Lama5	UTSW	2	179,840,603 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GATTGCCCAGACACTCGAAG -3'
(R):5'- ATGGGAGTGTCTCTGCGTAATC -3'

Sequencing Primer
(F):5'- GAAGACAACCCCAAGGCTGAG -3'
(R):5'- TCTCTGCGTAATCAGAAGGTGCAC -3'

Posted On

2019-05-13