Mutagenetix > Incidental Mutations

Incidental Mutation 'R7036:Med15'

546724

Institutional Source

Beutler Lab

Gene Symbol

Med15

Ensembl Gene

ENSMUSG00000012114

Gene Name

mediator complex subunit 15

Synonyms

Pcqap, A230074L19Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably essential (E-score: 0.959) question?

Stock #

R7036 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

17651208-17732891 bp(-) (GRCm38)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 17698155 bp

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 1 (M1K)

Ref Sequence

ENSEMBL: ENSMUSP00000156242 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000012259] [ENSMUST00000080936] [ENSMUST00000231413] [ENSMUST00000231674] [ENSMUST00000232202] [ENSMUST00000232236] [ENSMUST00000232431] [ENSMUST00000232645]

Predicted Effect

probably benign
Transcript: ENSMUST00000012259

SMART Domains

Protein: ENSMUSP00000012259
Gene: ENSMUSG00000012114

Domain	Start	End	E-Value	Type
Pfam:Med15	17	789	N/A	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000080936

SMART Domains

Protein: ENSMUSP00000079737
Gene: ENSMUSG00000012114

Domain	Start	End	E-Value	Type
Pfam:Med15	17	749	1.2e-276	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231413

Predicted Effect

probably benign
Transcript: ENSMUST00000231674

Predicted Effect

probably benign
Transcript: ENSMUST00000232163

Predicted Effect

probably benign
Transcript: ENSMUST00000232202

Predicted Effect

probably benign
Transcript: ENSMUST00000232236

Predicted Effect

probably benign
Transcript: ENSMUST00000232431

Predicted Effect

probably null
Transcript: ENSMUST00000232645
AA Change: M1K

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017N19Rik	T	A	10: 100,609,256		probably null	Het
9030624J02Rik	T	C	7: 118,773,092	S290P	probably damaging	Het
9130011E15Rik	A	G	19: 45,965,249	I195T	probably damaging	Het
Ahnak2	A	G	12: 112,778,781		probably benign	Het
Akt2	T	C	7: 27,637,012		probably null	Het
Aldh1a7	A	T	19: 20,708,178	L336Q	possibly damaging	Het
Ank2	T	C	3: 126,946,392		probably benign	Het
Ank3	C	T	10: 69,999,379	T680M	probably damaging	Het
Apeh	T	C	9: 108,094,271	E59G	possibly damaging	Het
Arl8a	G	C	1: 135,154,468	E145Q	probably benign	Het
Armc8	C	T	9: 99,483,965		probably null	Het
Arsj	T	C	3: 126,365,000	L76P	probably damaging	Het
Atp2b1	C	T	10: 98,987,310	T244I	probably damaging	Het
Bcl6	A	T	16: 23,974,861	L112Q	probably damaging	Het
Bfsp1	T	A	2: 143,826,923	T579S	possibly damaging	Het
Btaf1	C	A	19: 37,004,469	T1633K	probably benign	Het
Cacng8	A	G	7: 3,415,303	S324G	probably benign	Het
Cd2ap	A	T	17: 42,798,599	L623Q	probably damaging	Het
Cerkl	T	C	2: 79,341,378	I379V	probably benign	Het
Cndp2	T	A	18: 84,669,945	H307L	possibly damaging	Het
Derl1	A	G	15: 57,879,047		probably null	Het
Dixdc1	T	A	9: 50,682,564	R254S	probably benign	Het
Dopey2	T	A	16: 93,777,490	D30E	probably benign	Het
Duox2	T	G	2: 122,280,453	H1513P	probably damaging	Het
Dyrk1a	G	A	16: 94,686,568	V546I	probably benign	Het
E130308A19Rik	A	C	4: 59,719,991	K508Q	probably damaging	Het
Emc8	A	T	8: 120,659,051	V108E	probably benign	Het
Epb41l1	A	G	2: 156,529,402	T720A	probably benign	Het
Fam126b	T	A	1: 58,535,537	M282L	probably benign	Het
Fam71b	G	A	11: 46,407,408	S513N		Het
Fcgr4	T	A	1: 171,020,088	M85K	probably benign	Het
Fem1b	T	A	9: 62,797,028	I317F	probably damaging	Het
Focad	A	G	4: 88,124,637	E36G	probably benign	Het
Fryl	C	T	5: 73,055,608	E2275K	probably benign	Het
Galnt11	T	C	5: 25,258,813	I361T	probably damaging	Het
Gatad2a	A	T	8: 69,917,994	N114K	probably damaging	Het
Gcnt2	CTAATG	C	13: 40,887,556		probably null	Het
Gm884	T	A	11: 103,615,812		probably benign	Het
Gramd1a	A	G	7: 31,132,756		probably null	Het
Gsn	T	A	2: 35,292,599	W187R	probably damaging	Het
Hoxc12	G	A	15: 102,938,360	G229D	probably damaging	Het
Ints14	T	C	9: 64,964,545	V55A	probably benign	Het
Itga9	T	C	9: 118,698,365	L528P	probably benign	Het
Klf1	C	T	8: 84,902,750	S68F	possibly damaging	Het
Kmt2e	A	G	5: 23,478,743	E333G	probably null	Het
Krt39	A	C	11: 99,521,236	V8G	probably benign	Het
Krt71	A	G	15: 101,738,337	I312T	probably benign	Het
Lrp1b	T	C	2: 41,112,342	D2001G	possibly damaging	Het
Lrrc71	T	C	3: 87,748,386	T27A	probably benign	Het
Lrrcc1	T	C	3: 14,563,009	V958A	possibly damaging	Het
Mrgpra3	G	T	7: 47,590,090	N29K	possibly damaging	Het
Myl1	T	C	1: 66,930,236	N79S	probably damaging	Het
Naip2	A	T	13: 100,155,021	D1136E	probably benign	Het
Nat10	T	C	2: 103,754,108	N108S	probably benign	Het
Ncald	A	T	15: 37,368,878	S178T	probably benign	Het
Ndufs2	T	C	1: 171,238,308	D256G	probably benign	Het
Nek5	T	C	8: 22,107,723	N280S	probably benign	Het
Olfr1167	T	A	2: 88,149,125	D298V	probably damaging	Het
Olfr995	A	G	2: 85,438,430	S243P	probably damaging	Het
Parp1	G	T	1: 180,598,252	K849N	possibly damaging	Het
Pcdhb4	T	A	18: 37,308,782	C382S	possibly damaging	Het
Plce1	A	G	19: 38,739,357	N1520S	probably damaging	Het
Plcg2	G	A	8: 117,596,306	R700H	probably benign	Het
Popdc2	A	T	16: 38,362,811	Y52F	probably damaging	Het
Prrx1	T	A	1: 163,248,338	M220L	probably benign	Het
Pspc1	A	C	14: 56,758,628		probably null	Het
Ptchd1	T	A	X: 155,574,712	Y499F	probably damaging	Het
Ptpn20	A	G	14: 33,614,435	*44W	probably null	Het
Qars	T	A	9: 108,514,777	V83E	probably damaging	Het
Rrh	T	C	3: 129,815,693	E55G	possibly damaging	Het
Sash1	C	A	10: 8,730,083	E848*	probably null	Het
Sds	A	T	5: 120,480,847	K125M	possibly damaging	Het
Serpinf2	A	T	11: 75,438,418		probably benign	Het
Sh2b2	G	A	5: 136,218,885	T604I	probably benign	Het
Smug1	A	G	15: 103,155,942	L184P	probably damaging	Het
Spatc1	A	G	15: 76,283,880	T180A	probably benign	Het
Ssr1	A	T	13: 37,994,025	L20Q	probably null	Het
Supt4a	A	G	11: 87,743,258	E100G	probably damaging	Het
Tbx1	G	T	16: 18,586,801	P38T	unknown	Het
Tcl1	T	C	12: 105,217,601		probably benign	Het
Teddm2	T	C	1: 153,850,574	I132V	probably benign	Het
Tmem131	C	T	1: 36,792,973	G1861E	possibly damaging	Het
Tpr	A	T	1: 150,423,607	H1186L	probably benign	Het
Trim34b	A	T	7: 104,329,536		probably benign	Het
Trpm2	T	C	10: 77,912,592	M1415V	probably benign	Het
Ttll12	A	G	15: 83,586,885	F264S	probably benign	Het
Vmn2r59	T	C	7: 42,046,220	E256G	probably benign	Het
Vmn2r93	A	T	17: 18,326,410	H848L	probably benign	Het
Wdr70	T	C	15: 7,884,374	D598G	possibly damaging	Het
Ybey	T	C	10: 76,468,363	S2G	possibly damaging	Het
Zfp346	A	T	13: 55,132,387	Q308L	probably benign	Het
Zfp872	C	T	9: 22,200,560	P445L	probably benign	Het

Other mutations in Med15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00235:Med15	APN	16	17680726	missense	probably damaging	0.96
IGL00780:Med15	APN	16	17653487	missense	probably damaging	1.00
IGL02365:Med15	APN	16	17671606	intron	probably benign
R0324:Med15	UTSW	16	17697612	missense	probably damaging	0.98
R1225:Med15	UTSW	16	17722788	missense	probably damaging	1.00
R1695:Med15	UTSW	16	17722780	missense	probably damaging	0.96
R1745:Med15	UTSW	16	17655706	unclassified	probably benign
R1801:Med15	UTSW	16	17680735	missense	possibly damaging	0.66
R1838:Med15	UTSW	16	17653562	missense	probably benign	0.11
R1901:Med15	UTSW	16	17673154	unclassified	probably benign
R2153:Med15	UTSW	16	17685451	critical splice donor site	probably null
R2974:Med15	UTSW	16	17652711	missense	probably damaging	1.00
R3808:Med15	UTSW	16	17655734	unclassified	probably benign
R3809:Med15	UTSW	16	17655734	unclassified	probably benign
R4240:Med15	UTSW	16	17655494	missense	probably damaging	1.00
R4483:Med15	UTSW	16	17671564	intron	probably benign
R4484:Med15	UTSW	16	17671564	intron	probably benign
R4577:Med15	UTSW	16	17674515	nonsense	probably null
R5652:Med15	UTSW	16	17655191	missense	probably damaging	1.00
R6244:Med15	UTSW	16	17652745	nonsense	probably null
R6701:Med15	UTSW	16	17671583	intron	probably benign
R6793:Med15	UTSW	16	17652703	unclassified	probably benign
R7038:Med15	UTSW	16	17652727	missense	possibly damaging	0.90
R7211:Med15	UTSW	16	17698113	missense	unknown
R7317:Med15	UTSW	16	17671643	missense	unknown
R7390:Med15	UTSW	16	17722762	missense	unknown
R7471:Med15	UTSW	16	17722865	missense	probably benign	0.03
R7726:Med15	UTSW	16	17655174	missense	possibly damaging	0.87
Z1177:Med15	UTSW	16	17653232	missense	possibly damaging	0.81

Predicted Primers

PCR Primer
(F):5'- GACTGTCCCAAGAGATTGCTC -3'
(R):5'- ATAGCTCTTTCCCCTGGAGTG -3'

Sequencing Primer
(F):5'- CAGCACTATTGATTAGGTCAGTG -3'
(R):5'- TTCCCCTGGAGTGTGAAAAGGATATC -3'

Posted On

2019-05-13