Mutagenetix > Incidental Mutation

Incidental Mutation 'R7037:Zfp1005'

546747

Institutional Source

Beutler Lab

Gene Symbol

Zfp1005

Ensembl Gene

ENSMUSG00000079008

Gene Name

zinc finger protein 1005

Synonyms

Gm10749, EG640962, Gm14124

MMRRC Submission

045137-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R7037 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

150099437-150112220 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 150108376 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Phenylalanine at position 46 (V46F)

Ref Sequence

ENSEMBL: ENSMUSP00000105548 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109922]

AlphaFold

A2AU83

Predicted Effect

possibly damaging
Transcript: ENSMUST00000109922
AA Change: V46F

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000105548
Gene: ENSMUSG00000079008
AA Change: V46F

Domain	Start	End	E-Value	Type
KRAB	4	66	9.26e-19	SMART
ZnF_C2H2	103	125	7.49e-5	SMART
ZnF_C2H2	131	151	9.46e0	SMART
ZnF_C2H2	159	181	5.9e-3	SMART
ZnF_C2H2	187	209	6.67e-2	SMART
ZnF_C2H2	215	237	4.87e-4	SMART
ZnF_C2H2	243	265	2.82e0	SMART
ZnF_C2H2	271	293	2.2e2	SMART
ZnF_C2H2	299	321	1.4e-4	SMART
ZnF_C2H2	327	349	1.6e-4	SMART
ZnF_C2H2	355	377	1.18e-2	SMART
ZnF_C2H2	383	405	1.38e-3	SMART
ZnF_C2H2	411	433	9.56e1	SMART
ZnF_C2H2	439	461	6.99e-5	SMART
ZnF_C2H2	467	489	2.99e-4	SMART
ZnF_C2H2	495	517	7.78e-3	SMART
ZnF_C2H2	523	545	1.04e-3	SMART
ZnF_C2H2	551	573	1.6e-4	SMART
ZnF_C2H2	579	601	1.18e-2	SMART
ZnF_C2H2	607	629	4.54e-4	SMART
ZnF_C2H2	635	657	4.24e-4	SMART
ZnF_C2H2	663	685	1.2e-3	SMART
ZnF_C2H2	691	713	8.47e-4	SMART

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (70/70)

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsm3	A	T	7: 119,367,266 (GRCm39)	N33I	probably damaging	Het
Ahnak2	A	T	12: 112,740,712 (GRCm39)	V314D	probably damaging	Het
Arl14epl	T	G	18: 47,065,510 (GRCm39)	C92G	probably benign	Het
Atp6v1h	T	A	1: 5,220,215 (GRCm39)	M423K	possibly damaging	Het
Baiap3	G	A	17: 25,462,814 (GRCm39)	R1075C	probably benign	Het
Baz2b	C	T	2: 59,764,014 (GRCm39)		probably null	Het
Bicral	T	C	17: 47,135,560 (GRCm39)	H550R	probably benign	Het
C1rl	A	G	6: 124,485,598 (GRCm39)	Y323C	probably damaging	Het
Ccr9	T	A	9: 123,609,036 (GRCm39)	H239Q	possibly damaging	Het
Cdh16	T	A	8: 105,344,267 (GRCm39)	R91*	probably null	Het
Coro1c	A	G	5: 113,983,457 (GRCm39)	F357S	possibly damaging	Het
Cpsf4	G	A	5: 145,112,939 (GRCm39)	R141Q	possibly damaging	Het
Cryzl2	G	A	1: 157,298,318 (GRCm39)	V236I	probably damaging	Het
Cttnbp2	T	C	6: 18,435,117 (GRCm39)	E247G	probably damaging	Het
Dbr1	T	A	9: 99,458,621 (GRCm39)		probably null	Het
Dclk1	T	C	3: 55,370,469 (GRCm39)	S23P	probably damaging	Het
Dpyd	A	T	3: 118,692,938 (GRCm39)	I361F	probably benign	Het
Elac2	A	G	11: 64,874,537 (GRCm39)	E218G	probably benign	Het
Eml4	T	A	17: 83,732,756 (GRCm39)	D136E	probably benign	Het
Foxred1	A	T	9: 35,118,844 (GRCm39)	S223T	probably benign	Het
Garin5b	G	T	7: 4,761,584 (GRCm39)		probably benign	Het
Gask1a	G	T	9: 121,794,592 (GRCm39)	V249L	possibly damaging	Het
Gm11595	A	G	11: 99,663,474 (GRCm39)	C69R	unknown	Het
Gna14	A	T	19: 16,511,128 (GRCm39)	H59L		Het
H2-Ab1	T	A	17: 34,486,963 (GRCm39)	I239N	probably damaging	Het
Ints7	T	C	1: 191,351,717 (GRCm39)	S809P	probably benign	Het
Itgb4	T	A	11: 115,896,391 (GRCm39)	Y1379*	probably null	Het
Kank1	A	G	19: 25,407,705 (GRCm39)	D1233G	probably damaging	Het
Kif13a	C	T	13: 46,905,931 (GRCm39)	V671M	possibly damaging	Het
Lrrc66	A	T	5: 73,764,504 (GRCm39)	D846E	probably benign	Het
Lyst	A	G	13: 13,791,251 (GRCm39)	H38R	probably damaging	Het
Mc3r	T	C	2: 172,091,554 (GRCm39)	F259L	probably damaging	Het
Med25	A	G	7: 44,532,206 (GRCm39)	Y384H	probably damaging	Het
Met	A	T	6: 17,547,127 (GRCm39)		probably benign	Het
Mmp16	T	C	4: 18,116,148 (GRCm39)	V584A	possibly damaging	Het
Mrgprb3	A	G	7: 48,292,942 (GRCm39)	L203P	probably damaging	Het
Mus81	A	G	19: 5,536,108 (GRCm39)	L185P	probably damaging	Het
Naaa	A	G	5: 92,424,934 (GRCm39)	V75A	possibly damaging	Het
Obscn	T	A	11: 58,934,755 (GRCm39)	T5292S	probably damaging	Het
Obscn	T	C	11: 58,943,430 (GRCm39)	S4801G	probably damaging	Het
Or2ag19	T	C	7: 106,444,543 (GRCm39)	S242P	probably damaging	Het
Otof	T	C	5: 30,538,882 (GRCm39)	D1112G	probably benign	Het
Pals1	T	A	12: 78,843,973 (GRCm39)	I59N	probably damaging	Het
Pbx4	A	G	8: 70,317,525 (GRCm39)	R170G	probably damaging	Het
Pigt	CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT	CCAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGATCTGTAACCACAGGCCAGTGAGTAGGTTTGTCTCTGTCTAGTGTGGAT	2: 164,341,589 (GRCm39)		probably null	Het
Plce1	A	T	19: 38,690,461 (GRCm39)	D715V	probably damaging	Het
Pms1	T	C	1: 53,246,770 (GRCm39)	T311A	possibly damaging	Het
Popdc2	A	G	16: 38,194,629 (GRCm39)	D350G	probably damaging	Het
Prex1	A	G	2: 166,429,100 (GRCm39)	V661A	probably benign	Het
Ptbp2	A	G	3: 119,545,557 (GRCm39)	Y130H	probably damaging	Het
Rev3l	C	T	10: 39,727,971 (GRCm39)	R2707W	probably damaging	Het
Rpl37	G	A	15: 5,147,185 (GRCm39)	R75K	probably null	Het
Ryr3	T	A	2: 112,779,475 (GRCm39)	R259*	probably null	Het
Scai	A	T	2: 39,080,633 (GRCm39)	S8T	probably benign	Het
Scn4a	C	A	11: 106,211,726 (GRCm39)	L1430F	probably damaging	Het
Sema5a	A	G	15: 32,686,993 (GRCm39)	K1035R	probably damaging	Het
Siah3	A	G	14: 75,763,025 (GRCm39)	H92R	probably benign	Het
Smc4	G	A	3: 68,925,528 (GRCm39)	V342I	possibly damaging	Het
Spata31d1a	C	T	13: 59,848,138 (GRCm39)	C1330Y	possibly damaging	Het
St18	C	A	1: 6,873,260 (GRCm39)	H332N	possibly damaging	Het
Sycp1	T	A	3: 102,806,250 (GRCm39)	E480D	possibly damaging	Het
Tex14	A	T	11: 87,388,741 (GRCm39)	I323F	probably damaging	Het
Tm7sf2	A	T	19: 6,114,107 (GRCm39)		probably null	Het
Tmem241	G	T	18: 12,246,463 (GRCm39)	H62Q	probably benign	Het
Tmem54	T	A	4: 129,004,594 (GRCm39)		probably null	Het
Tomm34	A	G	2: 163,912,398 (GRCm39)	L39P	probably damaging	Het
Triml2	T	A	8: 43,646,573 (GRCm39)	V354D	probably damaging	Het
Usp19	T	C	9: 108,374,157 (GRCm39)	I738T	possibly damaging	Het
Utp25	A	C	1: 192,803,031 (GRCm39)		probably null	Het
Utrn	T	A	10: 12,702,514 (GRCm39)		probably null	Het

Other mutations in Zfp1005

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01550:Zfp1005	APN	2	150,108,363 (GRCm39)	splice site	probably benign
R0220:Zfp1005	UTSW	2	150,110,595 (GRCm39)	missense	unknown
R0396:Zfp1005	UTSW	2	150,109,973 (GRCm39)	missense	probably damaging	1.00
R0402:Zfp1005	UTSW	2	150,111,136 (GRCm39)	missense	possibly damaging	0.93
R0446:Zfp1005	UTSW	2	150,109,993 (GRCm39)	missense	possibly damaging	0.71
R0462:Zfp1005	UTSW	2	150,111,122 (GRCm39)	missense	possibly damaging	0.80
R0507:Zfp1005	UTSW	2	150,110,044 (GRCm39)	missense	possibly damaging	0.69
R0605:Zfp1005	UTSW	2	150,110,523 (GRCm39)	missense	unknown
R0838:Zfp1005	UTSW	2	150,111,220 (GRCm39)	missense	possibly damaging	0.74
R1327:Zfp1005	UTSW	2	150,108,070 (GRCm39)	missense	possibly damaging	0.71
R1405:Zfp1005	UTSW	2	150,109,620 (GRCm39)	nonsense	probably null
R1405:Zfp1005	UTSW	2	150,109,620 (GRCm39)	nonsense	probably null
R2114:Zfp1005	UTSW	2	150,109,819 (GRCm39)	missense	unknown
R2140:Zfp1005	UTSW	2	150,111,281 (GRCm39)	missense	probably benign	0.33
R3683:Zfp1005	UTSW	2	150,109,976 (GRCm39)	missense	probably benign	0.41
R3917:Zfp1005	UTSW	2	150,108,039 (GRCm39)	splice site	probably benign
R4084:Zfp1005	UTSW	2	150,108,122 (GRCm39)	missense	possibly damaging	0.85
R4499:Zfp1005	UTSW	2	150,111,362 (GRCm39)	missense	possibly damaging	0.93
R4683:Zfp1005	UTSW	2	150,108,390 (GRCm39)	missense	possibly damaging	0.53
R4762:Zfp1005	UTSW	2	150,109,549 (GRCm39)	missense	possibly damaging	0.53
R4937:Zfp1005	UTSW	2	150,110,680 (GRCm39)	missense	unknown
R5678:Zfp1005	UTSW	2	150,110,425 (GRCm39)	nonsense	probably null
R5696:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5697:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5698:Zfp1005	UTSW	2	150,111,394 (GRCm39)	missense	possibly damaging	0.52
R5769:Zfp1005	UTSW	2	150,110,198 (GRCm39)	missense	possibly damaging	0.87
R5780:Zfp1005	UTSW	2	150,108,139 (GRCm39)	missense	probably benign	0.05
R5973:Zfp1005	UTSW	2	150,109,855 (GRCm39)	missense	unknown
R6662:Zfp1005	UTSW	2	150,108,172 (GRCm39)	critical splice donor site	probably null
R6878:Zfp1005	UTSW	2	150,108,406 (GRCm39)	missense	possibly damaging	0.86
R7081:Zfp1005	UTSW	2	150,110,189 (GRCm39)	missense	possibly damaging	0.66
R7413:Zfp1005	UTSW	2	150,108,081 (GRCm39)	missense	possibly damaging	0.93
R7725:Zfp1005	UTSW	2	150,110,468 (GRCm39)	missense	unknown
R7781:Zfp1005	UTSW	2	150,109,577 (GRCm39)	missense	possibly damaging	0.53
R8197:Zfp1005	UTSW	2	150,109,577 (GRCm39)	missense	possibly damaging	0.53
R8355:Zfp1005	UTSW	2	150,109,876 (GRCm39)	missense	unknown
R8517:Zfp1005	UTSW	2	150,110,043 (GRCm39)	missense	probably benign	0.33
R8812:Zfp1005	UTSW	2	150,109,624 (GRCm39)	missense	possibly damaging	0.83
R9108:Zfp1005	UTSW	2	150,109,969 (GRCm39)	missense	possibly damaging	0.61
R9488:Zfp1005	UTSW	2	150,110,477 (GRCm39)	missense	unknown
R9499:Zfp1005	UTSW	2	150,109,856 (GRCm39)	missense	unknown
R9551:Zfp1005	UTSW	2	150,109,856 (GRCm39)	missense	unknown
R9567:Zfp1005	UTSW	2	150,109,517 (GRCm39)	missense	possibly damaging	0.53
R9646:Zfp1005	UTSW	2	150,110,104 (GRCm39)	missense	probably benign	0.43
R9709:Zfp1005	UTSW	2	150,110,305 (GRCm39)	missense	possibly damaging	0.47
R9719:Zfp1005	UTSW	2	150,111,304 (GRCm39)	missense	possibly damaging	0.74
R9779:Zfp1005	UTSW	2	150,108,064 (GRCm39)	missense	possibly damaging	0.92
X0022:Zfp1005	UTSW	2	150,109,578 (GRCm39)	missense	possibly damaging	0.53
Z1177:Zfp1005	UTSW	2	150,110,244 (GRCm39)	missense	possibly damaging	0.94
Z1177:Zfp1005	UTSW	2	150,110,237 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- TTTAGGGTAGAGTGACCTATGATGATG -3'
(R):5'- GCAATTCTTCAGCATAGCCCTATT -3'

Sequencing Primer
(F):5'- CTACAGGAATCTCGCTGCTATAGG -3'
(R):5'- ACTTAGAGGAATGGTTTGAAAACAC -3'

Posted On

2019-05-13