Mutagenetix > Incidental Mutation

Incidental Mutation 'R7041:Or5m10'

547039

Institutional Source

Beutler Lab

Gene Symbol

Or5m10

Ensembl Gene

ENSMUSG00000050128

Gene Name

olfactory receptor family 5 subfamily M member 10

Synonyms

GA_x6K02T2Q125-47363965-47364900, MOR196-3, Olfr1023

MMRRC Submission

045140-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R7041 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85717146-85718081 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 85717965 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 274 (I274F)

Ref Sequence

ENSEMBL: ENSMUSP00000149138 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056408] [ENSMUST00000213441]

AlphaFold

A2ASU6

Predicted Effect

probably benign
Transcript: ENSMUST00000056408
AA Change: I274F

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

SMART Domains

Protein: ENSMUSP00000059849
Gene: ENSMUSG00000050128
AA Change: I274F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.6e-52	PFAM
Pfam:7TM_GPCR_Srsx	35	304	2.6e-7	PFAM
Pfam:7tm_1	41	290	2.4e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000213441
AA Change: I274F

PolyPhen 2 Score 0.008 (Sensitivity: 0.96; Specificity: 0.76)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

96% (53/55)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	G	T	7: 78,748,096 (GRCm39)	E956*	probably null	Het
Adam25	A	T	8: 41,207,121 (GRCm39)	H129L	probably benign	Het
Adgrl4	A	T	3: 151,144,959 (GRCm39)	H36L	probably benign	Het
Ago1	T	A	4: 126,357,499 (GRCm39)	I59F	possibly damaging	Het
Anapc1	A	T	2: 128,470,576 (GRCm39)	V1518E	possibly damaging	Het
Atxn1	A	G	13: 45,720,311 (GRCm39)	I528T	probably damaging	Het
B4galnt4	A	G	7: 140,650,593 (GRCm39)	H820R	probably damaging	Het
Cacna1h	T	C	17: 25,612,977 (GRCm39)	E282G	probably damaging	Het
Camk1	T	A	6: 113,316,475 (GRCm39)	M95L	probably benign	Het
Capn7	C	T	14: 31,058,642 (GRCm39)		probably benign	Het
Cav1	A	G	6: 17,339,143 (GRCm39)	E45G	possibly damaging	Het
Ccdc183	T	G	2: 25,503,682 (GRCm39)	E185A	probably benign	Het
Ccl2	T	A	11: 81,926,489 (GRCm39)	M1K	probably null	Het
Cep97	T	A	16: 55,726,117 (GRCm39)	H590L	probably benign	Het
Dsg1c	A	T	18: 20,399,201 (GRCm39)	I102F	probably damaging	Het
Fcho2	A	G	13: 98,921,334 (GRCm39)	Y184H	possibly damaging	Het
Gart	C	T	16: 91,440,031 (GRCm39)		probably benign	Het
Gask1a	A	T	9: 121,794,467 (GRCm39)	Q207L	probably damaging	Het
Golga3	G	A	5: 110,356,450 (GRCm39)		probably null	Het
Hint3	G	T	10: 30,486,380 (GRCm39)	A133E	probably damaging	Het
Hspe1	T	C	1: 55,128,376 (GRCm39)		probably null	Het
Insr	A	T	8: 3,308,418 (GRCm39)	V206E	probably benign	Het
Insrr	T	C	3: 87,722,551 (GRCm39)	S1258P	probably damaging	Het
Itga11	C	T	9: 62,659,538 (GRCm39)	T430M	probably damaging	Het
Jmjd1c	G	A	10: 67,056,388 (GRCm39)	V890I	possibly damaging	Het
Kdm4b	T	A	17: 56,703,592 (GRCm39)	S717R	probably damaging	Het
Large1	A	T	8: 73,843,092 (GRCm39)	C144S	probably damaging	Het
Lrat	G	T	3: 82,810,755 (GRCm39)	Q89K	probably benign	Het
Lrrc66	A	T	5: 73,765,899 (GRCm39)	F381L	possibly damaging	Het
Myo15a	A	G	11: 60,396,832 (GRCm39)	T2634A	probably damaging	Het
Nup205	T	G	6: 35,201,470 (GRCm39)	I1182M	possibly damaging	Het
Or2a51	T	A	6: 43,178,837 (GRCm39)	D86E	probably benign	Het
Or6c66	T	A	10: 129,461,603 (GRCm39)	E109V	probably damaging	Het
Plekha6	T	A	1: 133,200,198 (GRCm39)	V259D	possibly damaging	Het
Prdm9	C	A	17: 15,765,257 (GRCm39)	A508S	possibly damaging	Het
Prickle2	A	G	6: 92,353,286 (GRCm39)	F783L	probably benign	Het
Ptprc	T	C	1: 138,054,047 (GRCm39)	S31G	probably benign	Het
Rbak	A	T	5: 143,159,226 (GRCm39)	I609N	probably damaging	Het
Rimklb	A	T	6: 122,436,176 (GRCm39)	L134*	probably null	Het
Ripor2	A	G	13: 24,877,749 (GRCm39)	I250V	probably benign	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Spaca6	C	T	17: 18,056,358 (GRCm39)	L118F	probably benign	Het
Tmem167	G	A	13: 90,246,533 (GRCm39)	C19Y	probably benign	Het
Togaram1	C	T	12: 65,067,160 (GRCm39)	T1684I	possibly damaging	Het
Trappc8	T	C	18: 21,007,729 (GRCm39)	T129A	probably benign	Het
Ubash3a	A	G	17: 31,447,184 (GRCm39)	S347G	probably benign	Het
Unc80	T	C	1: 66,542,752 (GRCm39)	S289P	probably benign	Het
Vmn2r11	A	G	5: 109,202,816 (GRCm39)	I87T	probably damaging	Het
Vmn2r54	A	T	7: 12,363,751 (GRCm39)	F381I	probably damaging	Het
Wdsub1	A	G	2: 59,683,224 (GRCm39)	L450P	probably damaging	Het
Xylt2	A	G	11: 94,558,408 (GRCm39)		probably null	Het
Zfp429	A	T	13: 67,538,830 (GRCm39)	C205S	probably damaging	Het
Zfp60	T	C	7: 27,448,451 (GRCm39)	I373T	probably benign	Het
Zfp738	A	G	13: 67,818,420 (GRCm39)	S524P	probably damaging	Het

Other mutations in Or5m10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01536:Or5m10	APN	2	85,717,944 (GRCm39)	missense	probably damaging	1.00
IGL01622:Or5m10	APN	2	85,717,306 (GRCm39)	missense	probably benign	0.01
IGL01623:Or5m10	APN	2	85,717,306 (GRCm39)	missense	probably benign	0.01
IGL01977:Or5m10	APN	2	85,717,711 (GRCm39)	missense	probably damaging	1.00
IGL02057:Or5m10	APN	2	85,717,275 (GRCm39)	missense	probably benign	0.00
IGL02555:Or5m10	APN	2	85,717,742 (GRCm39)	missense	probably benign	0.34
IGL03133:Or5m10	APN	2	85,717,478 (GRCm39)	missense	probably damaging	1.00
IGL03180:Or5m10	APN	2	85,717,740 (GRCm39)	missense	probably benign	0.00
R0415:Or5m10	UTSW	2	85,717,782 (GRCm39)	missense	possibly damaging	0.94
R1476:Or5m10	UTSW	2	85,717,592 (GRCm39)	nonsense	probably null
R1544:Or5m10	UTSW	2	85,717,615 (GRCm39)	missense	probably damaging	1.00
R2058:Or5m10	UTSW	2	85,717,296 (GRCm39)	missense	possibly damaging	0.48
R4096:Or5m10	UTSW	2	85,717,767 (GRCm39)	missense	probably damaging	0.98
R5055:Or5m10	UTSW	2	85,717,585 (GRCm39)	missense	probably benign	0.12
R5703:Or5m10	UTSW	2	85,717,783 (GRCm39)	missense	probably benign	0.06
R6297:Or5m10	UTSW	2	85,717,159 (GRCm39)	missense	probably benign	0.35
R7070:Or5m10	UTSW	2	85,718,034 (GRCm39)	missense	probably benign	0.13
R7563:Or5m10	UTSW	2	85,717,482 (GRCm39)	missense	probably damaging	0.98
R7777:Or5m10	UTSW	2	85,717,951 (GRCm39)	missense	possibly damaging	0.83
R7913:Or5m10	UTSW	2	85,718,074 (GRCm39)	missense	probably damaging	0.96
R9060:Or5m10	UTSW	2	85,717,920 (GRCm39)	missense	probably benign	0.06
R9789:Or5m10	UTSW	2	85,717,338 (GRCm39)	missense	probably damaging	1.00
S24628:Or5m10	UTSW	2	85,717,782 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- GTAGCTGGCTTTACTCTCTCAAG -3'
(R):5'- ACCAAGTTCTTTATCCAGCACATTG -3'

Sequencing Primer
(F):5'- GGCTTTACTCTCTCAAGTTCCC -3'
(R):5'- CCAGCACATTGTATCAGAAATGATC -3'

Posted On

2019-05-13